4ngg - Revision history

OCA at 13:03, 11 August 2016

OCA — Thu, 11 Aug 2016 13:03:20 GMT

←Older revision		Revision as of 13:03, 11 August 2016
Line 1:		Line 1:
		+
	==Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 13-mer siRNA having 5'-A and UU-3' ends (2.6 Angstrom resolution)==		==Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 13-mer siRNA having 5'-A and UU-3' ends (2.6 Angstrom resolution)==
	<StructureSection load='4ngg' size='340' side='right' caption='[[4ngg]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='4ngg' size='340' side='right' caption='[[4ngg]], [[Resolution\|resolution]] 2.60Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ngb\|4ngb]], [[4ngc\|4ngc]], [[4ngd\|4ngd]], [[4ngf\|4ngf]], [[4nh3\|4nh3]], [[4nh5\|4nh5]], [[4nh6\|4nh6]], [[4nha\|4nha]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ngb\|4ngb]], [[4ngc\|4ngc]], [[4ngd\|4ngd]], [[4ngf\|4ngf]], [[4nh3\|4nh3]], [[4nh5\|4nh5]], [[4nh6\|4nh6]], [[4nha\|4nha]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DICER, DICER1, HERNA, KIAA0928 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DICER, DICER1, HERNA, KIAA0928 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ngg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ngg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ngg RCSB], [http://www.ebi.ac.uk/pdbsum/4ngg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ngg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ngg OCA], [http://pdbe.org/4ngg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ngg RCSB], [http://www.ebi.ac.uk/pdbsum/4ngg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ngg ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 20:		Line 21:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4ngg" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ribonuclease\|Ribonuclease]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:02, 5 January 2015

OCA — Mon, 05 Jan 2015 09:02:15 GMT

←Older revision		Revision as of 09:02, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ngg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NGG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NGG FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ngg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NGG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NGG FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ngb\|4ngb]], [[4ngc\|4ngc]], [[4ngd\|4ngd]], [[4ngf\|4ngf]], [[4nh3\|4nh3]], [[4nh5\|4nh5]], [[4nh6\|4nh6]], [[4nha\|4nha]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ngb\|4ngb]], [[4ngc\|4ngc]], [[4ngd\|4ngd]], [[4ngf\|4ngf]], [[4nh3\|4nh3]], [[4nh5\|4nh5]], [[4nh6\|4nh6]], [[4nha\|4nha]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DICER, DICER1, HERNA, KIAA0928 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DICER, DICER1, HERNA, KIAA0928 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ngg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ngg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ngg RCSB], [http://www.ebi.ac.uk/pdbsum/4ngg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ngg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ngg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ngg RCSB], [http://www.ebi.ac.uk/pdbsum/4ngg PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Disease ==		== Disease ==
	[[http://www.uniprot.org/uniprot/DICER_HUMAN DICER_HUMAN]] Defects in DICER1 are a cause of pleuropulmonary blastoma (PPB) [MIM:[http://omim.org/entry/601200 601200]]. PPB is a rare pediatric tumor of the lung that arises during fetal lung development and is often part of an inherited cancer syndrome. PPBs contain both epithelial and mesenchymal cells. Early in tumorigenesis, cysts form in lung airspaces, and these cysts are lined with benign-appearing epithelium. Mesenchymal cells susceptible to malignant transformation reside within the cyst walls and form a dense 'cambium' layer beneath the epithelial lining. In a subset of patients, overgrowth of the mesenchymal cells produces a sarcoma, a transition that is associated with a poorer prognosis.<ref>PMID:21882293</ref> <ref>PMID:19556464</ref> Defects in DICER1 are the cause of goiter multinodular type 1 with or without Sertoli-Leydig cell tumors (MNG1) [MIM:[http://omim.org/entry/138800 138800]]. A common disorder characterized by nodular overgrowth of the thyroid gland. Some individuals may also develop Sertoli-Leydig cell tumors, usually of the ovary.<ref>PMID:21882293</ref> <ref>PMID:21205968</ref> Note=DICER1 mutations have been found in uterine cervix embryonal rhabdomyosarcoma, primitive neuroectodermal tumor, Wilms tumor, pulmonary sequestration and juvenile intestinal polyp (PubMed:21882293). Somatic missense mutations affecting the RNase IIIb domain of DICER1 are common in non-epithelial ovarian tumors. These mutations do not abolish DICER1 function but alter it in specific cell types, a novel mechanism through which perturbation of microRNA processing may be oncogenic (PubMed:22187960).<ref>PMID:21882293</ref>		[[http://www.uniprot.org/uniprot/DICER_HUMAN DICER_HUMAN]] Defects in DICER1 are a cause of pleuropulmonary blastoma (PPB) [MIM:[http://omim.org/entry/601200 601200]]. PPB is a rare pediatric tumor of the lung that arises during fetal lung development and is often part of an inherited cancer syndrome. PPBs contain both epithelial and mesenchymal cells. Early in tumorigenesis, cysts form in lung airspaces, and these cysts are lined with benign-appearing epithelium. Mesenchymal cells susceptible to malignant transformation reside within the cyst walls and form a dense 'cambium' layer beneath the epithelial lining. In a subset of patients, overgrowth of the mesenchymal cells produces a sarcoma, a transition that is associated with a poorer prognosis.<ref>PMID:21882293</ref> <ref>PMID:19556464</ref> Defects in DICER1 are the cause of goiter multinodular type 1 with or without Sertoli-Leydig cell tumors (MNG1) [MIM:[http://omim.org/entry/138800 138800]]. A common disorder characterized by nodular overgrowth of the thyroid gland. Some individuals may also develop Sertoli-Leydig cell tumors, usually of the ovary.<ref>PMID:21882293</ref> <ref>PMID:21205968</ref> Note=DICER1 mutations have been found in uterine cervix embryonal rhabdomyosarcoma, primitive neuroectodermal tumor, Wilms tumor, pulmonary sequestration and juvenile intestinal polyp (PubMed:21882293). Somatic missense mutations affecting the RNase IIIb domain of DICER1 are common in non-epithelial ovarian tumors. These mutations do not abolish DICER1 function but alter it in specific cell types, a novel mechanism through which perturbation of microRNA processing may be oncogenic (PubMed:22187960).<ref>PMID:21882293</ref>
Line 18:		Line 18:
	A Phosphate-Binding Pocket within the Platform-PAZ-Connector Helix Cassette of Human Dicer.,Tian Y, Simanshu DK, Ma JB, Park JE, Heo I, Kim VN, Patel DJ Mol Cell. 2014 Feb 20;53(4):606-16. doi: 10.1016/j.molcel.2014.01.003. Epub 2014 , Jan 30. PMID:24486018<ref>PMID:24486018</ref>		A Phosphate-Binding Pocket within the Platform-PAZ-Connector Helix Cassette of Human Dicer.,Tian Y, Simanshu DK, Ma JB, Park JE, Heo I, Kim VN, Patel DJ Mol Cell. 2014 Feb 20;53(4):606-16. doi: 10.1016/j.molcel.2014.01.003. Epub 2014 , Jan 30. PMID:24486018<ref>PMID:24486018</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
-	[[Category: Patel, D J.]]	+	[[Category: Patel, D J]]
-	[[Category: Simanshu, D K.]]	+	[[Category: Simanshu, D K]]
-	[[Category: Tian, Y.]]	+	[[Category: Tian, Y]]
	[[Category: Connector helix]]		[[Category: Connector helix]]
	[[Category: Endoribonuclease]]		[[Category: Endoribonuclease]]

OCA at 12:26, 18 May 2014

OCA — Sun, 18 May 2014 12:26:18 GMT

←Older revision		Revision as of 12:26, 18 May 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4ngg\| PDB=4ngg \| SCENE= }}~~	+	==Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 13-mer siRNA having 5'-A and UU-3' ends (2.6 Angstrom resolution)==
-	===Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 13-mer siRNA having 5'-A and UU-3' ends (2.6 Angstrom resolution)===	+	<StructureSection load='4ngg' size='340' side='right' caption='[[4ngg]], [[Resolution\|resolution]] 2.60Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24486018}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4ngg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NGG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NGG FirstGlance]. <br>
-	==Disease==	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ngb\|4ngb]], [[4ngc\|4ngc]], [[4ngd\|4ngd]], [[4ngf\|4ngf]], [[4nh3\|4nh3]], [[4nh5\|4nh5]], [[4nh6\|4nh6]], [[4nha\|4nha]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DICER, DICER1, HERNA, KIAA0928 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ngg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ngg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ngg RCSB], [http://www.ebi.ac.uk/pdbsum/4ngg PDBsum]</span></td></tr>
		+	<table>
		+	== Disease ==
	[[http://www.uniprot.org/uniprot/DICER_HUMAN DICER_HUMAN]] Defects in DICER1 are a cause of pleuropulmonary blastoma (PPB) [MIM:[http://omim.org/entry/601200 601200]]. PPB is a rare pediatric tumor of the lung that arises during fetal lung development and is often part of an inherited cancer syndrome. PPBs contain both epithelial and mesenchymal cells. Early in tumorigenesis, cysts form in lung airspaces, and these cysts are lined with benign-appearing epithelium. Mesenchymal cells susceptible to malignant transformation reside within the cyst walls and form a dense 'cambium' layer beneath the epithelial lining. In a subset of patients, overgrowth of the mesenchymal cells produces a sarcoma, a transition that is associated with a poorer prognosis.<ref>PMID:21882293</ref> <ref>PMID:19556464</ref> Defects in DICER1 are the cause of goiter multinodular type 1 with or without Sertoli-Leydig cell tumors (MNG1) [MIM:[http://omim.org/entry/138800 138800]]. A common disorder characterized by nodular overgrowth of the thyroid gland. Some individuals may also develop Sertoli-Leydig cell tumors, usually of the ovary.<ref>PMID:21882293</ref> <ref>PMID:21205968</ref> Note=DICER1 mutations have been found in uterine cervix embryonal rhabdomyosarcoma, primitive neuroectodermal tumor, Wilms tumor, pulmonary sequestration and juvenile intestinal polyp (PubMed:21882293). Somatic missense mutations affecting the RNase IIIb domain of DICER1 are common in non-epithelial ovarian tumors. These mutations do not abolish DICER1 function but alter it in specific cell types, a novel mechanism through which perturbation of microRNA processing may be oncogenic (PubMed:22187960).<ref>PMID:21882293</ref>		[[http://www.uniprot.org/uniprot/DICER_HUMAN DICER_HUMAN]] Defects in DICER1 are a cause of pleuropulmonary blastoma (PPB) [MIM:[http://omim.org/entry/601200 601200]]. PPB is a rare pediatric tumor of the lung that arises during fetal lung development and is often part of an inherited cancer syndrome. PPBs contain both epithelial and mesenchymal cells. Early in tumorigenesis, cysts form in lung airspaces, and these cysts are lined with benign-appearing epithelium. Mesenchymal cells susceptible to malignant transformation reside within the cyst walls and form a dense 'cambium' layer beneath the epithelial lining. In a subset of patients, overgrowth of the mesenchymal cells produces a sarcoma, a transition that is associated with a poorer prognosis.<ref>PMID:21882293</ref> <ref>PMID:19556464</ref> Defects in DICER1 are the cause of goiter multinodular type 1 with or without Sertoli-Leydig cell tumors (MNG1) [MIM:[http://omim.org/entry/138800 138800]]. A common disorder characterized by nodular overgrowth of the thyroid gland. Some individuals may also develop Sertoli-Leydig cell tumors, usually of the ovary.<ref>PMID:21882293</ref> <ref>PMID:21205968</ref> Note=DICER1 mutations have been found in uterine cervix embryonal rhabdomyosarcoma, primitive neuroectodermal tumor, Wilms tumor, pulmonary sequestration and juvenile intestinal polyp (PubMed:21882293). Somatic missense mutations affecting the RNase IIIb domain of DICER1 are common in non-epithelial ovarian tumors. These mutations do not abolish DICER1 function but alter it in specific cell types, a novel mechanism through which perturbation of microRNA processing may be oncogenic (PubMed:22187960).<ref>PMID:21882293</ref>
-		+	== Function ==
-	==Function==	+
	[[http://www.uniprot.org/uniprot/DICER_HUMAN DICER_HUMAN]] Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.<ref>PMID:15242644</ref> <ref>PMID:16271387</ref> <ref>PMID:16289642</ref> <ref>PMID:16142218</ref> <ref>PMID:16357216</ref> <ref>PMID:15973356</ref> <ref>PMID:16424907</ref> <ref>PMID:17452327</ref> <ref>PMID:18178619</ref> <ref>PMID:19219043</ref>		[[http://www.uniprot.org/uniprot/DICER_HUMAN DICER_HUMAN]] Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.<ref>PMID:15242644</ref> <ref>PMID:16271387</ref> <ref>PMID:16289642</ref> <ref>PMID:16142218</ref> <ref>PMID:16357216</ref> <ref>PMID:15973356</ref> <ref>PMID:16424907</ref> <ref>PMID:17452327</ref> <ref>PMID:18178619</ref> <ref>PMID:19219043</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We have solved two families of crystal structures of the human Dicer "platform-PAZ-connector helix" cassette in complex with small interfering RNAs (siRNAs). The structures possess two adjacently positioned pockets: a 2 nt 3'-overhang-binding pocket within the PAZ domain (3' pocket) and a phosphate-binding pocket within the platform domain (phosphate pocket). One family of complexes contains a knob-like alpha-helical protrusion, designated "hDicer-specific helix," that separates the two pockets and orients the bound siRNA away from the surface of Dicer, which could be indicative of a product release/transfer state. In the second complex, the helical protrusion is melted/disordered and the bound siRNA is aligned toward the surface of Dicer, suggestive of a cleavage-competent state. These structures allow us to propose that the transition from the cleavage-competent to the postulated product release/transfer state may involve release of the 5'-phosphate from the phosphate pocket while retaining the 3' overhang in the 3' pocket.

-	~~==About this Structure==~~	+	A Phosphate-Binding Pocket within the Platform-PAZ-Connector Helix Cassette of Human Dicer.,Tian Y, Simanshu DK, Ma JB, Park JE, Heo I, Kim VN, Patel DJ Mol Cell. 2014 Feb 20;53(4):606-16. doi: 10.1016/j.molcel.2014.01.003. Epub 2014 , Jan 30. PMID:24486018<ref>PMID:24486018</ref>
-	~~[[4ngg]] is a 2 chain structure~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.ac.~~il/oca-bin/ocashort?id=4NGG OCA]~~.	+

-	==~~Reference~~==	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024486018</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Human]]
	[[Category: Patel, D J.]]		[[Category: Patel, D J.]]
	[[Category: Simanshu, D K.]]		[[Category: Simanshu, D K.]]

OCA at 13:31, 12 March 2014

OCA — Wed, 12 Mar 2014 13:31:57 GMT

←Older revision		Revision as of 13:31, 12 March 2014
Line 1:		Line 1:
-	''~~'Unreleased structure'''~~	+	{{STRUCTURE_4ngg\| PDB=4ngg \| SCENE= }}
		+	===Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 13-mer siRNA having 5'-A and UU-3' ends (2.6 Angstrom resolution)===
		+	{{ABSTRACT_PUBMED_24486018}}

-	~~The~~ entry ~~4ngg~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Disease==
		+	[[http://www.uniprot.org/uniprot/DICER_HUMAN DICER_HUMAN]] Defects in DICER1 are a cause of pleuropulmonary blastoma (PPB) [MIM:[http://omim.org/entry/601200 601200]]. PPB is a rare pediatric tumor of the lung that arises during fetal lung development and is often part of an inherited cancer syndrome. PPBs contain both epithelial and mesenchymal cells. Early in tumorigenesis, cysts form in lung airspaces, and these cysts are lined with benign-appearing epithelium. Mesenchymal cells susceptible to malignant transformation reside within the cyst walls and form a dense 'cambium' layer beneath the epithelial lining. In a subset of patients, overgrowth of the mesenchymal cells produces a sarcoma, a transition that is associated with a poorer prognosis.<ref>PMID:21882293</ref> <ref>PMID:19556464</ref> Defects in DICER1 are the cause of goiter multinodular type 1 with or without Sertoli-Leydig cell tumors (MNG1) [MIM:[http://omim.org/entry/138800 138800]]. A common disorder characterized by nodular overgrowth of the thyroid gland. Some individuals may also develop Sertoli-Leydig cell tumors, usually of the ovary.<ref>PMID:21882293</ref> <ref>PMID:21205968</ref> Note=DICER1 mutations have been found in uterine cervix embryonal rhabdomyosarcoma, primitive neuroectodermal tumor, Wilms tumor, pulmonary sequestration and juvenile intestinal polyp (PubMed:21882293). Somatic missense mutations affecting the RNase IIIb domain of DICER1 are common in non-epithelial ovarian tumors. These mutations do not abolish DICER1 function but alter it in specific cell types, a novel mechanism through which perturbation of microRNA processing may be oncogenic (PubMed:22187960).<ref>PMID:21882293</ref>

-	~~Authors~~: ~~Simanshu, D~~.K., ~~Tian~~, Y., ~~Patel, D~~.J.	+	==Function==
		+	[[http://www.uniprot.org/uniprot/DICER_HUMAN DICER_HUMAN]] Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.<ref>PMID:15242644</ref> <ref>PMID:16271387</ref> <ref>PMID:16289642</ref> <ref>PMID:16142218</ref> <ref>PMID:16357216</ref> <ref>PMID:15973356</ref> <ref>PMID:16424907</ref> <ref>PMID:17452327</ref> <ref>PMID:18178619</ref> <ref>PMID:19219043</ref>

-	~~Description:~~ Structure ~~of human Dicer Platform-PAZ~~-Connector ~~Helix cassette in~~ complex ~~with 13~~-~~mer siRNA having 5'-A and UU-3' ends (2.6 Angstrom resolution)~~	+	==About this Structure==
		+	[[4ngg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NGG OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:024486018</ref><references group="xtra"/><references/>
		+	[[Category: Patel, D J.]]
		+	[[Category: Simanshu, D K.]]
		+	[[Category: Tian, Y.]]
		+	[[Category: Connector helix]]
		+	[[Category: Endoribonuclease]]
		+	[[Category: Hydrolase-rna complex]]
		+	[[Category: Paz domain]]
		+	[[Category: Platform domain]]
		+	[[Category: Pre-mirna]]
		+	[[Category: Rnase iii domain]]
		+	[[Category: Sirna]]

OCA at 09:23, 25 December 2013

OCA — Wed, 25 Dec 2013 09:23:28 GMT

←Older revision		Revision as of 09:23, 25 December 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4ngg is ON HOLD	+	The entry 4ngg is ON HOLD until Paper Publication

	Authors: Simanshu, D.K., Tian, Y., Patel, D.J.		Authors: Simanshu, D.K., Tian, Y., Patel, D.J.

	Description: Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 13-mer siRNA having 5'-A and UU-3' ends (2.6 Angstrom resolution)		Description: Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 13-mer siRNA having 5'-A and UU-3' ends (2.6 Angstrom resolution)

OCA: Protected "4ngg" [edit=sysop:move=sysop]

OCA — Wed, 13 Nov 2013 07:47:57 GMT

Protected "4ngg" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:47, 13 November 2013

OCA: New page: '''Unreleased structure''' The entry 4ngg is ON HOLD Authors: Simanshu, D.K., Tian, Y., Patel, D.J. Description: Structure of human Dicer Platform-PAZ-Connector Helix cassette in compl...

OCA — Wed, 13 Nov 2013 07:47:56 GMT

New page: '''Unreleased structure''' The entry 4ngg is ON HOLD Authors: Simanshu, D.K., Tian, Y., Patel, D.J. Description: Structure of human Dicer Platform-PAZ-Connector Helix cassette in compl...

New page

'''Unreleased structure'''

The entry 4ngg is ON HOLD

Authors: Simanshu, D.K., Tian, Y., Patel, D.J.

Description: Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 13-mer siRNA having 5'-A and UU-3' ends (2.6 Angstrom resolution)