4ni6 - Revision history

OCA at 16:57, 20 September 2023

OCA — Wed, 20 Sep 2023 16:57:36 GMT

←Older revision		Revision as of 16:57, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ni6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_perfringens_B_str._ATCC_3626 Clostridium perfringens B str. ATCC 3626]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NI6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NI6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ni6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_perfringens_B_str._ATCC_3626 Clostridium perfringens B str. ATCC 3626]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NI6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NI6 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ni6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ni6 OCA], [https://pdbe.org/4ni6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ni6 RCSB], [https://www.ebi.ac.uk/pdbsum/4ni6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ni6 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.1Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ni6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ni6 OCA], [https://pdbe.org/4ni6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ni6 RCSB], [https://www.ebi.ac.uk/pdbsum/4ni6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ni6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 08:29, 11 January 2023

OCA — Wed, 11 Jan 2023 08:29:39 GMT

←Older revision		Revision as of 08:29, 11 January 2023
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	==Repeat domain 1 of Clostridium perfringens CPE0147==		==Repeat domain 1 of Clostridium perfringens CPE0147==
-	<StructureSection load='4ni6' size='340' side='right' caption='[[4ni6]], [[Resolution\|resolution]] 1.10Å' scene=''>	+	<StructureSection load='4ni6' size='340' side='right'caption='[[4ni6]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ni6]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Clostridium_perfringens_b_str~~.~~_atcc_3626~~ Clostridium perfringens b str. ~~atcc~~ 3626]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NI6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4NI6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ni6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_perfringens_B_str._ATCC_3626 Clostridium perfringens B str. ATCC 3626]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NI6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NI6 FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4mkm\|4mkm]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ni6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ni6 OCA], [https://pdbe.org/4ni6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ni6 RCSB], [https://www.ebi.ac.uk/pdbsum/4ni6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ni6 ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AC1_0147 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=451754 Clostridium perfringens B str. ATCC 3626])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ni6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ni6 OCA], [~~http~~://pdbe.org/4ni6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ni6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ni6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ni6 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B1R775_CLOPF B1R775_CLOPF]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Clostridium perfringens b str. ~~atcc~~ 3626]]	+	[[Category: Clostridium perfringens B str. ATCC 3626]]
-	[[Category: ~~Baker, E N~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Kwon, H~~]]	+	[[Category: Baker EN]]
-	[[Category: ~~Squire, C J~~]]	+	[[Category: Kwon H]]
-	[[Category: ~~Young, P G~~]]	+	[[Category: Squire CJ]]
-	[[Category: ~~Igg-like fold]]~~	+	[[Category: Young PG]]
-	~~[[Category: Internal ester crosslink]]~~	+
-	~~[[Category: Unknown function~~]]	+

OCA at 10:02, 4 August 2016

OCA — Thu, 04 Aug 2016 10:02:56 GMT

←Older revision		Revision as of 10:02, 4 August 2016
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		+
	==Repeat domain 1 of Clostridium perfringens CPE0147==		==Repeat domain 1 of Clostridium perfringens CPE0147==
	<StructureSection load='4ni6' size='340' side='right' caption='[[4ni6]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='4ni6' size='340' side='right' caption='[[4ni6]], [[Resolution\|resolution]] 1.10Å' scene=''>
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	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4mkm\|4mkm]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4mkm\|4mkm]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AC1_0147 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=451754 Clostridium perfringens B str. ATCC 3626])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AC1_0147 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=451754 Clostridium perfringens B str. ATCC 3626])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ni6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ni6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ni6 RCSB], [http://www.ebi.ac.uk/pdbsum/4ni6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ni6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ni6 OCA], [http://pdbe.org/4ni6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ni6 RCSB], [http://www.ebi.ac.uk/pdbsum/4ni6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ni6 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4ni6" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:42, 21 December 2014

OCA — Sun, 21 Dec 2014 16:42:30 GMT

←Older revision		Revision as of 16:42, 21 December 2014
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-	~~{{STRUCTURE_4ni6\| PDB~~=4ni6 \| ~~SCENE~~= }}	+	==Repeat domain 1 of Clostridium perfringens CPE0147==
-	===~~Repeat domain~~ 1 of Clostridium perfringens ~~CPE0147~~===	+	<StructureSection load='4ni6' size='340' side='right' caption='[[4ni6]], [[Resolution\|resolution]] 1.10Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24344302}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4ni6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_perfringens_b_str._atcc_3626 Clostridium perfringens b str. atcc 3626]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NI6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NI6 FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4mkm\|4mkm]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AC1_0147 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=451754 Clostridium perfringens B str. ATCC 3626])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ni6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ni6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ni6 RCSB], [http://www.ebi.ac.uk/pdbsum/4ni6 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Gram-positive bacteria are decorated by a variety of proteins that are anchored to the cell wall and project from it to mediate colonization, attachment to host cells, and pathogenesis. These proteins, and protein assemblies, such as pili, are typically long and thin yet must withstand high levels of mechanical stress and proteolytic attack. The recent discovery of intramolecular isopeptide bond cross-links, formed autocatalytically, in the pili from Streptococcus pyogenes has highlighted the role that such cross-links can play in stabilizing such structures. We have investigated a putative cell-surface adhesin from Clostridium perfringens comprising an N-terminal adhesin domain followed by 11 repeat domains. The crystal structure of a two-domain fragment shows that each domain has an IgG-like fold and contains an unprecedented ester bond joining Thr and Gln side chains. MS confirms the presence of these bonds. We show that the bonds form through an autocatalytic intramolecular reaction catalyzed by an adjacent His residue in a serine protease-like mechanism. Two buried acidic residues assist in the reaction. By mutagenesis, we show that loss of the ester bond reduces the thermal stability drastically and increases susceptibility to proteolysis. As in pilin domains, the bonds are placed at a strategic position joining the first and last strands, even though the Ig fold type differs. Bioinformatic analysis suggests that similar domains and ester bond cross-links are widespread in Gram-positive bacterial adhesins.

-	~~==About this Structure==~~	+	Autocatalytically generated Thr-Gln ester bond cross-links stabilize the repetitive Ig-domain shaft of a bacterial cell surface adhesin.,Kwon H, Squire CJ, Young PG, Baker EN Proc Natl Acad Sci U S A. 2013 Dec 16. PMID:24344302<ref>PMID:24344302</ref>
-	~~[[4ni6]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Clostridium_perfringens_b_str~~.~~_atcc_3626 Clostridium perfringens b str. atcc 3626]. Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4NI6 OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024344302</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Clostridium perfringens b str. atcc 3626]]		[[Category: Clostridium perfringens b str. atcc 3626]]
-	[[Category: Baker, E N.]]	+	[[Category: Baker, E N]]
-	[[Category: Kwon, H.]]	+	[[Category: Kwon, H]]
-	[[Category: Squire, C J.]]	+	[[Category: Squire, C J]]
-	[[Category: Young, P G.]]	+	[[Category: Young, P G]]
	[[Category: Igg-like fold]]		[[Category: Igg-like fold]]
	[[Category: Internal ester crosslink]]		[[Category: Internal ester crosslink]]
	[[Category: Unknown function]]		[[Category: Unknown function]]

OCA at 10:51, 1 January 2014

OCA — Wed, 01 Jan 2014 10:51:16 GMT

←Older revision		Revision as of 10:51, 1 January 2014
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	{{STRUCTURE_4ni6\| PDB=4ni6 \| SCENE= }}		{{STRUCTURE_4ni6\| PDB=4ni6 \| SCENE= }}
	===Repeat domain 1 of Clostridium perfringens CPE0147===		===Repeat domain 1 of Clostridium perfringens CPE0147===
		+	{{ABSTRACT_PUBMED_24344302}}

	==About this Structure==		==About this Structure==
-	[[4ni6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NI6 OCA].	+	[[4ni6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_perfringens_b_str._atcc_3626 Clostridium perfringens b str. atcc 3626]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NI6 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:024344302</ref><references group="xtra"/><references/>
		+	[[Category: Clostridium perfringens b str. atcc 3626]]
	[[Category: Baker, E N.]]		[[Category: Baker, E N.]]
	[[Category: Kwon, H.]]		[[Category: Kwon, H.]]

OCA at 07:34, 4 December 2013

OCA — Wed, 04 Dec 2013 07:34:53 GMT

←Older revision		Revision as of 07:34, 4 December 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4ni6\| PDB=4ni6 \| SCENE= }}
		+	===Repeat domain 1 of Clostridium perfringens CPE0147===

-	~~The entry~~ 4ni6 is ~~ON HOLD~~	+	==About this Structure==
-		+	[[4ni6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NI6 OCA].
-	~~Authors~~: Kwon, H., Squire, C.J., Young, P.G.~~, Baker, E.N.~~	+	[[Category: Baker, E N.]]
-		+	[[Category: Kwon, H.]]
-	~~Description~~: ~~Repeat domain 1 of Clostridium perfringens CPE0147~~	+	[[Category: Squire, C J.]]
		+	[[Category: Young, P G.]]
		+	[[Category: Igg-like fold]]
		+	[[Category: Internal ester crosslink]]
		+	[[Category: Unknown function]]

OCA: Protected "4ni6" [edit=sysop:move=sysop]

OCA — Wed, 13 Nov 2013 07:50:13 GMT

Protected "4ni6" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:50, 13 November 2013

OCA: New page: '''Unreleased structure''' The entry 4ni6 is ON HOLD Authors: Kwon, H., Squire, C.J., Young, P.G., Baker, E.N. Description: Repeat domain 1 of Clostridium perfringens CPE0147

OCA — Wed, 13 Nov 2013 07:50:12 GMT

New page: '''Unreleased structure''' The entry 4ni6 is ON HOLD Authors: Kwon, H., Squire, C.J., Young, P.G., Baker, E.N. Description: Repeat domain 1 of Clostridium perfringens CPE0147

New page

'''Unreleased structure'''

The entry 4ni6 is ON HOLD

Authors: Kwon, H., Squire, C.J., Young, P.G., Baker, E.N.

Description: Repeat domain 1 of Clostridium perfringens CPE0147