4njq - Revision history

OCA at 08:56, 20 March 2024

OCA — Wed, 20 Mar 2024 08:56:59 GMT

←Older revision		Revision as of 08:56, 20 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4njq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NJQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NJQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4njq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NJQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NJQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.702Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4njq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4njq OCA], [https://pdbe.org/4njq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4njq RCSB], [https://www.ebi.ac.uk/pdbsum/4njq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4njq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4njq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4njq OCA], [https://pdbe.org/4njq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4njq RCSB], [https://www.ebi.ac.uk/pdbsum/4njq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4njq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/APEB_PSEAE APEB_PSEAE]		[https://www.uniprot.org/uniprot/APEB_PSEAE APEB_PSEAE]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The peptidases in clan MH are known as cocatalytic zinc peptidases that have two zinc ions in the active site, but their metal preference has not been rigorously investigated. In this study, the molecular basis for metal preference is provided from the structural and biochemical analyses. Kinetic studies of Pseudomonas aeruginosa aspartyl aminopeptidase (PaAP) which belongs to peptidase family M18 in clan MH revealed that its peptidase activity is dependent on Co2+ rather than Zn2+: the kcat (s-1) values of PaAP were 0.006, 5.10 and 0.43 in no-metal, Co2+, and Zn2+conditions, respectively. Consistently, addition of low concentrations of Co2+ to PaAP previously saturated with Zn2+ greatly enhanced the enzymatic activity, suggesting that Co2+may be the physiologically relevant cocatalytic metal ion of PaAP. The crystal structures of PaAP complexes with Co2+ or Zn2+ commonly showed two metal ions in the active site coordinated with three conserved residues and a bicarbonate ion in a tetragonal geometry. However, Co2+- and Zn2+-bound structures showed no noticeable alterations relevant to differential effects of metal species, except the relative orientation of Glu-265, a general base in the active site. The characterization of mutant PaAP revealed that the first metal binding site is primarily responsible for metal preference. Similar to PaAP, Streptococcus pneumonia glutamyl aminopeptidase (SpGP), belonging to aminopeptidase family M42 in clan MH, also showed requirement for Co2+ for maximum activity. These results proposed that clan MH peptidases might be a cocatalytic cobalt peptidase rather than a zinc-dependent peptidase.
-
-	Structural and kinetic bases for the metal preference of the M18 aminopeptidase from Pseudomonas aeruginosa.,Nguyen DD, Pandian R, Kim D, Ha SC, Yoon HJ, Kim KS, Yun KH, Kim JH, Kim KK Biochem Biophys Res Commun. 2014 Apr 1. pii: S0006-291X(14)00561-0. doi:, 10.1016/j.bbrc.2014.03.109. PMID:24704201<ref>PMID:24704201</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4njq" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:31, 11 January 2023

OCA — Wed, 11 Jan 2023 08:31:23 GMT

←Older revision		Revision as of 08:31, 11 January 2023
Line 3:		Line 3:
	<StructureSection load='4njq' size='340' side='right'caption='[[4njq]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='4njq' size='340' side='right'caption='[[4njq]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4njq]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pseae Pseae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NJQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4NJQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4njq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NJQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NJQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4njr\|4njr]], [[4oid\|4oid]], [[4oiw\|4oiw]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4njq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4njq OCA], [https://pdbe.org/4njq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4njq RCSB], [https://www.ebi.ac.uk/pdbsum/4njq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4njq ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">apeB, PA3247 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4njq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4njq OCA], [~~http~~://pdbe.org/4njq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4njq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4njq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4njq ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/APEB_PSEAE APEB_PSEAE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pseae~~]]	+	[[Category: Pseudomonas aeruginosa PAO1]]
-	[[Category: Ha~~, S C~~]]	+	[[Category: Ha SC]]
-	[[Category: Kim~~, D Y~~]]	+	[[Category: Kim DY]]
-	[[Category: Kim~~, J H~~]]	+	[[Category: Kim JH]]
-	[[Category: Kim~~, K K~~]]	+	[[Category: Kim KK]]
-	[[Category: Kim~~, K S~~]]	+	[[Category: Kim KS]]
-	[[Category: Nguyen~~, D D~~]]	+	[[Category: Nguyen DD]]
-	[[Category: Pandian, R]]	+	[[Category: Pandian R]]
-	[[Category: Yun~~, K H]]~~	+	[[Category: Yun KH]]
-	~~[[Category: Aspartyl aminopeptidase]]~~	+
-	~~[[Category: Cobalt complex]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Metal binding]]~~	+
-	~~[[Category: Metallopeptidase]]~~	+
-	~~[[Category: Tetrahedral shape dodecameric structure~~]]	+

OCA at 15:58, 20 November 2019

OCA — Wed, 20 Nov 2019 15:58:31 GMT

←Older revision		Revision as of 15:58, 20 November 2019
Line 1:		Line 1:

	==Structural and kinetic bases for the metal preference of the M18 aminopeptidase from Pseudomonas aeruginosa==		==Structural and kinetic bases for the metal preference of the M18 aminopeptidase from Pseudomonas aeruginosa==
-	<StructureSection load='4njq' size='340' side='right' caption='[[4njq]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='4njq' size='340' side='right'caption='[[4njq]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4njq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NJQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NJQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4njq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NJQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NJQ FirstGlance]. <br>
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Pseae]]		[[Category: Pseae]]
	[[Category: Ha, S C]]		[[Category: Ha, S C]]

OCA at 17:10, 2 January 2017

OCA — Mon, 02 Jan 2017 17:10:00 GMT

←Older revision		Revision as of 17:10, 2 January 2017
Line 1:		Line 1:
		+
	==Structural and kinetic bases for the metal preference of the M18 aminopeptidase from Pseudomonas aeruginosa==		==Structural and kinetic bases for the metal preference of the M18 aminopeptidase from Pseudomonas aeruginosa==
	<StructureSection load='4njq' size='340' side='right' caption='[[4njq]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='4njq' size='340' side='right' caption='[[4njq]], [[Resolution\|resolution]] 2.70Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4njr\|4njr]], [[4oid\|4oid]], [[4oiw\|4oiw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4njr\|4njr]], [[4oid\|4oid]], [[4oiw\|4oiw]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">apeB, PA3247 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">apeB, PA3247 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4njq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4njq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4njq RCSB], [http://www.ebi.ac.uk/pdbsum/4njq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4njq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4njq OCA], [http://pdbe.org/4njq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4njq RCSB], [http://www.ebi.ac.uk/pdbsum/4njq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4njq ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4njq" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:23, 20 January 2015

OCA — Tue, 20 Jan 2015 09:23:42 GMT

←Older revision		Revision as of 09:23, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4njq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NJQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NJQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4njq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NJQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NJQ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4njr\|4njr]], [[4oid\|4oid]], [[4oiw\|4oiw]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4njr\|4njr]], [[4oid\|4oid]], [[4oiw\|4oiw]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">apeB, PA3247 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">apeB, PA3247 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4njq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4njq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4njq RCSB], [http://www.ebi.ac.uk/pdbsum/4njq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4njq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4njq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4njq RCSB], [http://www.ebi.ac.uk/pdbsum/4njq PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Pseae]]		[[Category: Pseae]]
-	[[Category: Ha, S C.]]	+	[[Category: Ha, S C]]
-	[[Category: Kim, D Y.]]	+	[[Category: Kim, D Y]]
-	[[Category: Kim, J H.]]	+	[[Category: Kim, J H]]
-	[[Category: Kim, K K.]]	+	[[Category: Kim, K K]]
-	[[Category: Kim, K S.]]	+	[[Category: Kim, K S]]
-	[[Category: Nguyen, D D.]]	+	[[Category: Nguyen, D D]]
-	[[Category: Pandian, R.]]	+	[[Category: Pandian, R]]
-	[[Category: Yun, K H.]]	+	[[Category: Yun, K H]]
	[[Category: Aspartyl aminopeptidase]]		[[Category: Aspartyl aminopeptidase]]
	[[Category: Cobalt complex]]		[[Category: Cobalt complex]]

OCA at 06:23, 24 September 2014

OCA — Wed, 24 Sep 2014 06:23:33 GMT

←Older revision		Revision as of 06:23, 24 September 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4njq\| PDB=4njq \| SCENE= }}~~	+	==Structural and kinetic bases for the metal preference of the M18 aminopeptidase from Pseudomonas aeruginosa==
-	===Structural and kinetic bases for the metal preference of the M18 aminopeptidase from Pseudomonas aeruginosa===	+	<StructureSection load='4njq' size='340' side='right' caption='[[4njq]], [[Resolution\|resolution]] 2.70Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24704201}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4njq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NJQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NJQ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4njr\|4njr]], [[4oid\|4oid]], [[4oiw\|4oiw]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">apeB, PA3247 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4njq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4njq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4njq RCSB], [http://www.ebi.ac.uk/pdbsum/4njq PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The peptidases in clan MH are known as cocatalytic zinc peptidases that have two zinc ions in the active site, but their metal preference has not been rigorously investigated. In this study, the molecular basis for metal preference is provided from the structural and biochemical analyses. Kinetic studies of Pseudomonas aeruginosa aspartyl aminopeptidase (PaAP) which belongs to peptidase family M18 in clan MH revealed that its peptidase activity is dependent on Co2+ rather than Zn2+: the kcat (s-1) values of PaAP were 0.006, 5.10 and 0.43 in no-metal, Co2+, and Zn2+conditions, respectively. Consistently, addition of low concentrations of Co2+ to PaAP previously saturated with Zn2+ greatly enhanced the enzymatic activity, suggesting that Co2+may be the physiologically relevant cocatalytic metal ion of PaAP. The crystal structures of PaAP complexes with Co2+ or Zn2+ commonly showed two metal ions in the active site coordinated with three conserved residues and a bicarbonate ion in a tetragonal geometry. However, Co2+- and Zn2+-bound structures showed no noticeable alterations relevant to differential effects of metal species, except the relative orientation of Glu-265, a general base in the active site. The characterization of mutant PaAP revealed that the first metal binding site is primarily responsible for metal preference. Similar to PaAP, Streptococcus pneumonia glutamyl aminopeptidase (SpGP), belonging to aminopeptidase family M42 in clan MH, also showed requirement for Co2+ for maximum activity. These results proposed that clan MH peptidases might be a cocatalytic cobalt peptidase rather than a zinc-dependent peptidase.

-	~~==About this Structure==~~	+	Structural and kinetic bases for the metal preference of the M18 aminopeptidase from Pseudomonas aeruginosa.,Nguyen DD, Pandian R, Kim D, Ha SC, Yoon HJ, Kim KS, Yun KH, Kim JH, Kim KK Biochem Biophys Res Commun. 2014 Apr 1. pii: S0006-291X(14)00561-0. doi:, 10.1016/j.bbrc.2014.03.109. PMID:24704201<ref>PMID:24704201</ref>
-	~~[[4njq]] is a 4 chain structure with sequence~~ from ~~[http://en~~.~~wikipedia~~.~~org/wiki/Pseae Pseae]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4NJQ OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024704201</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Pseae]]		[[Category: Pseae]]
	[[Category: Ha, S C.]]		[[Category: Ha, S C.]]

OCA at 06:56, 23 April 2014

OCA — Wed, 23 Apr 2014 06:56:35 GMT

←Older revision		Revision as of 06:56, 23 April 2014
Line 1:		Line 1:
	{{STRUCTURE_4njq\| PDB=4njq \| SCENE= }}		{{STRUCTURE_4njq\| PDB=4njq \| SCENE= }}
	===Structural and kinetic bases for the metal preference of the M18 aminopeptidase from Pseudomonas aeruginosa===		===Structural and kinetic bases for the metal preference of the M18 aminopeptidase from Pseudomonas aeruginosa===
		+	{{ABSTRACT_PUBMED_24704201}}

	==About this Structure==		==About this Structure==
-	[[4njq]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NJQ OCA].	+	[[4njq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NJQ OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:024704201</ref><references group="xtra"/><references/>
		+	[[Category: Pseae]]
	[[Category: Ha, S C.]]		[[Category: Ha, S C.]]
	[[Category: Kim, D Y.]]		[[Category: Kim, D Y.]]

OCA at 09:36, 2 April 2014

OCA — Wed, 02 Apr 2014 09:36:28 GMT

←Older revision		Revision as of 09:36, 2 April 2014
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4njq\| PDB=4njq \| SCENE= }}
		+	===Structural and kinetic bases for the metal preference of the M18 aminopeptidase from Pseudomonas aeruginosa===

-	~~The entry~~ 4njq is ~~ON HOLD~~	+	==About this Structure==
-		+	[[4njq]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NJQ OCA].
-	~~Authors~~: ~~Nguyen, D~~.D.~~, Pandian, R~~.~~, Kim, D~~.~~Y.,~~ Ha, S.C., ~~Yun, K~~.H., Kim, K.S., ~~Kim~~, ~~J.H~~.~~, Kim~~, K.K.	+	[[Category: Ha, S C.]]
-		+	[[Category: Kim, D Y.]]
-	~~Description~~: ~~Structural and kinetic bases for the metal preference of the M18~~ aminopeptidase ~~from Pseudomonas aeruginosa~~	+	[[Category: Kim, J H.]]
		+	[[Category: Kim, K K.]]
		+	[[Category: Kim, K S.]]
		+	[[Category: Nguyen, D D.]]
		+	[[Category: Pandian, R.]]
		+	[[Category: Yun, K H.]]
		+	[[Category: Aspartyl aminopeptidase]]
		+	[[Category: Cobalt complex]]
		+	[[Category: Hydrolase]]
		+	[[Category: Metal binding]]
		+	[[Category: Metallopeptidase]]
		+	[[Category: Tetrahedral shape dodecameric structure]]

OCA: Protected "4njq" [edit=sysop:move=sysop]

OCA — Wed, 20 Nov 2013 12:59:49 GMT

Protected "4njq" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:59, 20 November 2013

OCA: New page: '''Unreleased structure''' The entry 4njq is ON HOLD Authors: Nguyen, D.D., Pandian, R., Kim, D.Y., Ha, S.C., Yun, K.H., Kim, K.S., Kim, J.H., Kim, K.K. Description: Structural and kin...

OCA — Wed, 20 Nov 2013 12:59:48 GMT

New page: '''Unreleased structure''' The entry 4njq is ON HOLD Authors: Nguyen, D.D., Pandian, R., Kim, D.Y., Ha, S.C., Yun, K.H., Kim, K.S., Kim, J.H., Kim, K.K. Description: Structural and kin...

New page

'''Unreleased structure'''

The entry 4njq is ON HOLD

Authors: Nguyen, D.D., Pandian, R., Kim, D.Y., Ha, S.C., Yun, K.H., Kim, K.S., Kim, J.H., Kim, K.K.

Description: Structural and kinetic bases for the metal preference of the M18 aminopeptidase from Pseudomonas aeruginosa