4nk5 - Revision history

OCA at 16:58, 20 September 2023

OCA — Wed, 20 Sep 2023 16:58:22 GMT

←Older revision		Revision as of 16:58, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4nk5]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Liberibacter_asiaticus Candidatus Liberibacter asiaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NK5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NK5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4nk5]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Liberibacter_asiaticus Candidatus Liberibacter asiaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NK5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NK5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nk5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nk5 OCA], [https://pdbe.org/4nk5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nk5 RCSB], [https://www.ebi.ac.uk/pdbsum/4nk5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nk5 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nk5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nk5 OCA], [https://pdbe.org/4nk5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nk5 RCSB], [https://www.ebi.ac.uk/pdbsum/4nk5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nk5 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:31, 11 January 2023

OCA — Wed, 11 Jan 2023 08:31:49 GMT

←Older revision		Revision as of 08:31, 11 January 2023
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	==Crystal structure of FabI-NAD complex from Candidatus Liberibacter asiaticus==		==Crystal structure of FabI-NAD complex from Candidatus Liberibacter asiaticus==
-	<StructureSection load='4nk5' size='340' side='right' caption='[[4nk5]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='4nk5' size='340' side='right'caption='[[4nk5]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4nk5]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"candidatus_liberobacter_asiaticum"_jagoueix_et_al._1994 "candidatus liberobacter asiaticum" jagoueix et al. 1994~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NK5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4NK5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4nk5]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Liberibacter_asiaticus Candidatus Liberibacter asiaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NK5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NK5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4nk4\|4nk4]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nk5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nk5 OCA], [https://pdbe.org/4nk5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nk5 RCSB], [https://www.ebi.ac.uk/pdbsum/4nk5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nk5 ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WSI_01645 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34021 "Candidatus Liberobacter asiaticum" Jagoueix et al. 1994])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Enoyl-[acyl-carrier-protein]_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.9 1.3.1.9] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4nk5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nk5 OCA], [~~http~~://pdbe.org/4nk5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4nk5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4nk5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4nk5 ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	==See Also==		==See Also==
-	*[[Enoyl-Acyl-Carrier Protein Reductase\|Enoyl-Acyl-Carrier Protein Reductase]]	+	*[[Enoyl-Acyl-Carrier Protein Reductase 3D structures\|Enoyl-Acyl-Carrier Protein Reductase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Candidatus ~~liberobacter asiaticum jagoueix et al. 1994~~]]	+	[[Category: Candidatus Liberibacter asiaticus]]
-	[[Category: ~~Dong, Y H~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Gao, Z Q~~]]	+	[[Category: Dong YH]]
-	[[Category: ~~Jiang, L~~]]	+	[[Category: Gao ZQ]]
-	[[Category: ~~Enoyl-acp reductase i]]~~	+	[[Category: Jiang L]]
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 10:20, 11 August 2016

OCA — Thu, 11 Aug 2016 10:20:04 GMT

←Older revision		Revision as of 10:20, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of FabI-NAD complex from Candidatus Liberibacter asiaticus==		==Crystal structure of FabI-NAD complex from Candidatus Liberibacter asiaticus==
	<StructureSection load='4nk5' size='340' side='right' caption='[[4nk5]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='4nk5' size='340' side='right' caption='[[4nk5]], [[Resolution\|resolution]] 2.70Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WSI_01645 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34021 "Candidatus Liberobacter asiaticum" Jagoueix et al. 1994])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WSI_01645 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34021 "Candidatus Liberobacter asiaticum" Jagoueix et al. 1994])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Enoyl-[acyl-carrier-protein]_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.9 1.3.1.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Enoyl-[acyl-carrier-protein]_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.9 1.3.1.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nk5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nk5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nk5 RCSB], [http://www.ebi.ac.uk/pdbsum/4nk5 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nk5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nk5 OCA], [http://pdbe.org/4nk5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4nk5 RCSB], [http://www.ebi.ac.uk/pdbsum/4nk5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4nk5 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4nk5" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 09:35, 5 January 2015

OCA — Mon, 05 Jan 2015 09:35:06 GMT

←Older revision		Revision as of 09:35, 5 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_4nk5\| PDB=4nk5 \| SCENE= }}~~	+	==Crystal structure of FabI-NAD complex from Candidatus Liberibacter asiaticus==
-	===Crystal structure of FabI-NAD complex from Candidatus Liberibacter asiaticus===	+	<StructureSection load='4nk5' size='340' side='right' caption='[[4nk5]], [[Resolution\|resolution]] 2.70Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24407918}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4nk5]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"candidatus_liberobacter_asiaticum"_jagoueix_et_al._1994 "candidatus liberobacter asiaticum" jagoueix et al. 1994]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NK5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NK5 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4nk4\|4nk4]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WSI_01645 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34021 "Candidatus Liberobacter asiaticum" Jagoueix et al. 1994])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Enoyl-[acyl-carrier-protein]_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.9 1.3.1.9] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nk5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nk5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nk5 RCSB], [http://www.ebi.ac.uk/pdbsum/4nk5 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Huanglongbing (HLB) is a destructive citrus disease. The leading cause of HLB is Candidatus Liberibacter asiaticus. Fatty acid biosynthesis(FAS-II)is essential for bacterial viability and has been validated as a target for the discovery of novel antibacterials agents. Enoyl-acyl carrier protein reductase (also called ENR or FabI and a product of the fabI gene) is an enzyme required in a critical step of bacterial fatty acid biosynthesis and has attracted attention as a target of novel antimicrobial agents. We determined the crystal structures of FabI from Ca. L. asiaticus in its apo-form as well as in complex with b-nicotinamide adenine dinucleotide (NAD) at 1.7 and 2.7 A resolutions, respectively, to facilitate the design and screening of small molecule inhibitors of FabI. The monomeric ClFabI is highly similar to other known FabI structures as expected, however, Unlike the typical tetramer, ClFabI exists as hexamer in crystal whereas as dimer in solution, on the other hand, the substrate binding loop which always disordered in apo form, FabI structures is ordered in apo ClFabI. Interestingly, the structure of ClFabI undergoes remarkable conformational change in the substrate-binding loop in the presence of NAD. We conclude that the signature sequence motif of FabI can be considered as Gly-(Xaa)5 -Ser-(Xaa)n-Val-Tyr-(Xaa)6 -Lys-(Xaa)n-Thr instead of Tyr-(Xaa)6 -Lys. We have further identified isoniazid as a competitive inhibitor with NADH.

-	~~==About this Structure==~~	+	Crystal structures and kinetic properties of enoyl-acyl carrier protein reductase I from Candidatus Liberibacter asiaticus.,Jiang L, Gao Z, Li Y, Wang S, Dong Y Protein Sci. 2014 Jan 9. doi: 10.1002/pro.2418. PMID:24407918<ref>PMID:24407918</ref>
-	~~[[4nk5]] is a 6 chain structure. Full crystallographic information is available~~ from ~~[http~~:/~~/oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin~~/~~ocashort?id=4NK5 OCA]~~.	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Enoyl-Acyl-Carrier Protein Reductase\|Enoyl-Acyl-Carrier Protein Reductase]]		*[[Enoyl-Acyl-Carrier Protein Reductase\|Enoyl-Acyl-Carrier Protein Reductase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:024407918</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
-	[[Category: Dong, Y H.]]	+	</StructureSection>
-	[[Category: Gao, Z Q.]]	+	[[Category: Candidatus liberobacter asiaticum jagoueix et al. 1994]]
-	[[Category: Jiang, L.]]	+	[[Category: Dong, Y H]]
		+	[[Category: Gao, Z Q]]
		+	[[Category: Jiang, L]]
	[[Category: Enoyl-acp reductase i]]		[[Category: Enoyl-acp reductase i]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 04:36, 9 April 2014

OCA — Wed, 09 Apr 2014 04:36:22 GMT

←Older revision		Revision as of 04:36, 9 April 2014
Line 5:		Line 5:
	==About this Structure==		==About this Structure==
	[[4nk5]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NK5 OCA].		[[4nk5]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NK5 OCA].
		+
		+	==See Also==
		+	*[[Enoyl-Acyl-Carrier Protein Reductase\|Enoyl-Acyl-Carrier Protein Reductase]]

	==Reference==		==Reference==

OCA at 05:01, 13 February 2014

OCA — Thu, 13 Feb 2014 05:01:31 GMT

←Older revision		Revision as of 05:01, 13 February 2014
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4nk5\| PDB=4nk5 \| SCENE= }}
		+	===Crystal structure of FabI-NAD complex from Candidatus Liberibacter asiaticus===
		+	{{ABSTRACT_PUBMED_24407918}}

-	~~The entry~~ 4nk5 is ~~ON HOLD~~	+	==About this Structure==
		+	[[4nk5]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NK5 OCA].

-	~~Authors~~: ~~Jiang~~, L., Gao, Z.Q., ~~Dong, Y~~.H.	+	==Reference==
-		+	<ref group="xtra">PMID:024407918</ref><references group="xtra"/><references/>
-	~~Description~~: ~~Crystal structure of FabI~~-~~NAD complex from Candidatus Liberibacter asiaticus~~	+	[[Category: Dong, Y H.]]
		+	[[Category: Gao, Z Q.]]
		+	[[Category: Jiang, L.]]
		+	[[Category: Enoyl-acp reductase i]]
		+	[[Category: Oxidoreductase]]

OCA: Protected "4nk5" [edit=sysop:move=sysop]

OCA — Wed, 20 Nov 2013 13:00:21 GMT

Protected "4nk5" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:00, 20 November 2013

OCA: New page: '''Unreleased structure''' The entry 4nk5 is ON HOLD Authors: Jiang, L., Gao, Z.Q., Dong, Y.H. Description: Crystal structure of FabI-NAD complex from Candidatus Liberibacter asiaticus

OCA — Wed, 20 Nov 2013 13:00:20 GMT

New page: '''Unreleased structure''' The entry 4nk5 is ON HOLD Authors: Jiang, L., Gao, Z.Q., Dong, Y.H. Description: Crystal structure of FabI-NAD complex from Candidatus Liberibacter asiaticus

New page

'''Unreleased structure'''

The entry 4nk5 is ON HOLD

Authors: Jiang, L., Gao, Z.Q., Dong, Y.H.

Description: Crystal structure of FabI-NAD complex from Candidatus Liberibacter asiaticus