4nrz - Revision history

OCA at 08:36, 18 January 2023

OCA — Wed, 18 Jan 2023 08:36:00 GMT

←Older revision		Revision as of 08:36, 18 January 2023
Line 3:		Line 3:
	<StructureSection load='4nrz' size='340' side='right'caption='[[4nrz]], [[Resolution\|resolution]] 2.42Å' scene=''>		<StructureSection load='4nrz' size='340' side='right'caption='[[4nrz]], [[Resolution\|resolution]] 2.42Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4nrz]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NRZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4NRZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4nrz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NRZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NRZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4nrx\|4nrx]], [[4nry\|4nry]]~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nrz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nrz OCA], [https://pdbe.org/4nrz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nrz RCSB], [https://www.ebi.ac.uk/pdbsum/4nrz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nrz ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4nrz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nrz OCA], [~~http~~://pdbe.org/4nrz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4nrz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4nrz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4nrz ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kwong~~, P D~~]]	+	[[Category: Kwong PD]]
-	[[Category: Ofek, G]]	+	[[Category: Ofek G]]
-	[[Category: Yang, Y]]	+	[[Category: Yang Y]]
-	~~[[Category: Hiv-1 gp41]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Immunoglobulin]]~~	+
-	~~[[Category: Membrane proximal external region]]~~	+
-	~~[[Category: Mper]]~~	+
-	~~[[Category: Neutralizing antibody~~]]	+

OCA at 05:25, 13 February 2020

OCA — Thu, 13 Feb 2020 05:25:49 GMT

←Older revision		Revision as of 05:25, 13 February 2020
Line 1:		Line 1:

	==Crystal Structure of HIV-1 Neutralizing Antibody m66.6==		==Crystal Structure of HIV-1 Neutralizing Antibody m66.6==
-	<StructureSection load='4nrz' size='340' side='right' caption='[[4nrz]], [[Resolution\|resolution]] 2.42Å' scene=''>	+	<StructureSection load='4nrz' size='340' side='right'caption='[[4nrz]], [[Resolution\|resolution]] 2.42Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4nrz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NRZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NRZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4nrz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NRZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NRZ FirstGlance]. <br>
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Kwong, P D]]		[[Category: Kwong, P D]]
	[[Category: Ofek, G]]		[[Category: Ofek, G]]

OCA at 20:14, 11 August 2016

OCA — Thu, 11 Aug 2016 20:14:53 GMT

←Older revision		Revision as of 20:14, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of HIV-1 Neutralizing Antibody m66.6==		==Crystal Structure of HIV-1 Neutralizing Antibody m66.6==
	<StructureSection load='4nrz' size='340' side='right' caption='[[4nrz]], [[Resolution\|resolution]] 2.42Å' scene=''>		<StructureSection load='4nrz' size='340' side='right' caption='[[4nrz]], [[Resolution\|resolution]] 2.42Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4nrx\|4nrx]], [[4nry\|4nry]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4nrx\|4nrx]], [[4nry\|4nry]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nrz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nrz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nrz RCSB], [http://www.ebi.ac.uk/pdbsum/4nrz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nrz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nrz OCA], [http://pdbe.org/4nrz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4nrz RCSB], [http://www.ebi.ac.uk/pdbsum/4nrz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4nrz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4nrz" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:01, 5 January 2015

OCA — Mon, 05 Jan 2015 09:01:36 GMT

←Older revision		Revision as of 09:01, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4nrz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NRZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NRZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4nrz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NRZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NRZ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4nrx\|4nrx]], [[4nry\|4nry]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4nrx\|4nrx]], [[4nry\|4nry]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nrz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nrz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nrz RCSB], [http://www.ebi.ac.uk/pdbsum/4nrz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nrz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nrz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nrz RCSB], [http://www.ebi.ac.uk/pdbsum/4nrz PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 13:
	Structural Basis for HIV-1 Neutralization by 2F5-like Antibodies m66 and m66.6.,Ofek G, Zirkle B, Yang Y, Zhu Z, McKee K, Zhang B, Chuang GY, Georgiev IS, O'Dell S, Doria-Rose N, Mascola JR, Dimitrov DS, Kwong PD J Virol. 2013 Dec 11. PMID:24335316<ref>PMID:24335316</ref>		Structural Basis for HIV-1 Neutralization by 2F5-like Antibodies m66 and m66.6.,Ofek G, Zirkle B, Yang Y, Zhu Z, McKee K, Zhang B, Chuang GY, Georgiev IS, O'Dell S, Doria-Rose N, Mascola JR, Dimitrov DS, Kwong PD J Virol. 2013 Dec 11. PMID:24335316<ref>PMID:24335316</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
-	[[Category: Kwong, P D.]]	+	[[Category: Kwong, P D]]
-	[[Category: Ofek, G.]]	+	[[Category: Ofek, G]]
-	[[Category: Yang, Y.]]	+	[[Category: Yang, Y]]
	[[Category: Hiv-1 gp41]]		[[Category: Hiv-1 gp41]]
	[[Category: Immune system]]		[[Category: Immune system]]

OCA at 13:39, 18 May 2014

OCA — Sun, 18 May 2014 13:39:41 GMT

←Older revision		Revision as of 13:39, 18 May 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4nrz\| PDB=4nrz \| SCENE= }}~~	+	==Crystal Structure of HIV-1 Neutralizing Antibody m66.6==
-	===Crystal Structure of HIV-1 Neutralizing Antibody m66.6===	+	<StructureSection load='4nrz' size='340' side='right' caption='[[4nrz]], [[Resolution\|resolution]] 2.42Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24335316}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4nrz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NRZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NRZ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4nrx\|4nrx]], [[4nry\|4nry]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nrz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nrz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nrz RCSB], [http://www.ebi.ac.uk/pdbsum/4nrz PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Antibodies m66.6 and 2F5 are the only effective human HIV-1-neutralizing antibodies identified thus far that recognize the N-terminal region of the membrane-proximal external region (MPER) of the gp41 subunit of the HIV-1 viral spike. Although 2F5 has been extensively characterized, much less is known about antibody m66.6 or antibody m66, a closely related light-chain variant. Here, we report the crystal structure of m66 in complex with its gp41 epitope, along with unbound structures of m66 and m66.6. We employed mutational and binding analyses to decipher antibody elements critical for recognition of their gp41 epitopes, and determined the molecular basis that underlies their neutralization of HIV-1. When bound by m66, the N-terminal region of the gp41 MPER adopts a conformation comprising a helix followed by an extended loop. Comparison of gp41-bound m66 with unbound m66.6 identified three light chain residues of m66.6 that were confirmed through mutagenesis to underlie the superior breadth and potency of m66.6-mediated virus neutralization. Recognition of gp41 by m66 also revealed similarities to antibody 2F5 both in the conformation of crucial epitope residues as well as in the angles of antibody approach. Aromatic residues at the tip of the m66.6-heavy chain third complementarity-determining region, as in the case of 2F5, were determined to be critical for virus neutralization in a manner that correlated with antibody recognition of the MPER in a lipid context. Antibodies m66, m66.6, and 2F5 thus utilize similar mechanistic elements to recognize a common gp41-MPER epitope and neutralize HIV-1.

-	~~==About this Structure==~~	+	Structural Basis for HIV-1 Neutralization by 2F5-like Antibodies m66 and m66.6.,Ofek G, Zirkle B, Yang Y, Zhu Z, McKee K, Zhang B, Chuang GY, Georgiev IS, O'Dell S, Doria-Rose N, Mascola JR, Dimitrov DS, Kwong PD J Virol. 2013 Dec 11. PMID:24335316<ref>PMID:24335316</ref>
-	~~[[4nrz]] is a 4 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4NRZ OCA].~~	+

-	==~~Reference~~==	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024335316</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Human]]
	[[Category: Kwong, P D.]]		[[Category: Kwong, P D.]]
	[[Category: Ofek, G.]]		[[Category: Ofek, G.]]

OCA at 11:15, 5 February 2014

OCA — Wed, 05 Feb 2014 11:15:09 GMT

←Older revision		Revision as of 11:15, 5 February 2014
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4nrz\| PDB=4nrz \| SCENE= }}
		+	===Crystal Structure of HIV-1 Neutralizing Antibody m66.6===
		+	{{ABSTRACT_PUBMED_24335316}}

-	~~The entry~~ 4nrz is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
		+	[[4nrz]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NRZ OCA].

-	~~Authors~~: Ofek, G., Yang, Y.~~, Kwong, P.D.~~	+	==Reference==
-		+	<ref group="xtra">PMID:024335316</ref><references group="xtra"/><references/>
-	~~Description~~: ~~Crystal Structure of HIV~~-1 Neutralizing ~~Antibody m66.6~~	+	[[Category: Kwong, P D.]]
		+	[[Category: Ofek, G.]]
		+	[[Category: Yang, Y.]]
		+	[[Category: Hiv-1 gp41]]
		+	[[Category: Immune system]]
		+	[[Category: Immunoglobulin]]
		+	[[Category: Membrane proximal external region]]
		+	[[Category: Mper]]
		+	[[Category: Neutralizing antibody]]

OCA at 09:17, 8 January 2014

OCA — Wed, 08 Jan 2014 09:17:11 GMT

←Older revision		Revision as of 09:17, 8 January 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4nrz is ON HOLD	+	The entry 4nrz is ON HOLD until Paper Publication

	Authors: Ofek, G., Yang, Y., Kwong, P.D.		Authors: Ofek, G., Yang, Y., Kwong, P.D.

	Description: Crystal Structure of HIV-1 Neutralizing Antibody m66.6		Description: Crystal Structure of HIV-1 Neutralizing Antibody m66.6

OCA: Protected "4nrz" [edit=sysop:move=sysop]

OCA — Wed, 04 Dec 2013 06:54:16 GMT

Protected "4nrz" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:54, 4 December 2013

OCA: New page: '''Unreleased structure''' The entry 4nrz is ON HOLD Authors: Ofek, G., Yang, Y., Kwong, P.D. Description: Crystal Structure of HIV-1 Neutralizing Antibody m66.6

OCA — Wed, 04 Dec 2013 06:54:15 GMT

New page: '''Unreleased structure''' The entry 4nrz is ON HOLD Authors: Ofek, G., Yang, Y., Kwong, P.D. Description: Crystal Structure of HIV-1 Neutralizing Antibody m66.6

New page

'''Unreleased structure'''

The entry 4nrz is ON HOLD

Authors: Ofek, G., Yang, Y., Kwong, P.D.

Description: Crystal Structure of HIV-1 Neutralizing Antibody m66.6