4nx3 - Revision history

OCA: Redirecting to 4web

OCA — Wed, 17 Dec 2014 12:31:38 GMT

Redirecting to 4web

←Older revision		Revision as of 12:31, 17 December 2014
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-	~~==Structure of the core ectodomain of the hepatitis C virus envelope glycoprotein 2==~~	+	#REDIRECT [[4web]] This PDB entry is obsolete and replaced by 4web
-	~~<StructureSection load='4nx3' size='340' side='right' caption='~~[[~~4nx3~~]]~~, [[Resolution\|resolution]] 2.40Å' scene=''>~~	+
-	~~== Structural highlights ==~~	+
-	<table><tr><td colspan='2'>[[4nx3]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Hepatitis_c_virus_subtype_2a Hepatitis c virus subtype 2a] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NX3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NX3 FirstGlance]. <br>	+
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ARF:FORMAMIDE'>ARF</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>	+
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nx3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nx3 RCSB], [http://www.ebi.ac.uk/pdbsum/4nx3 PDBsum]</span></td></tr>	+
-	~~<table>~~	+
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Hepatitis C virus (HCV) is a significant public health concern with approximately 160 million people infected worldwide. HCV infection often results in chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. No vaccine is available and current therapies are effective against some, but not all, genotypes. HCV is an enveloped virus with two surface glycoproteins (E1 and E2). E2 binds to the host cell through interactions with scavenger receptor class B type I (SR-BI) and CD81, and serves as a target for neutralizing antibodies. Little is known about the molecular mechanism that mediates cell entry ~~and membrane fusion, although E2~~ is predicted to be a class II viral fusion protein. Here we describe the structure of the E2 core domain in complex with an antigen-binding fragment (Fab) at 2.4 A resolution. The E2 core has a compact, globular domain structure, consisting mostly of beta-strands and ~~random coil with two small alpha-helices. The strands are arranged in two, perpendicular sheets (A and B), which are held together~~ by an extensive hydrophobic core and disulphide bonds. Sheet A has an IgG-like fold that is commonly found in viral and cellular proteins, whereas sheet B represents a novel fold. Solution-based studies demonstrate that the full-length E2 ectodomain has a similar globular architecture and does not undergo significant conformational or oligomeric rearrangements on exposure to low pH. Thus, the IgG-like fold is the only feature that E2 shares with class II membrane fusion proteins. These results provide unprecedented insights into HCV entry and will assist in developing an HCV vaccine and new inhibitors.	+
-		+
-	Structure of the core ectodomain of the hepatitis C virus envelope glycoprotein 2.,Khan AG, Whidby J, Miller MT, Scarborough H, Zatorski AV, Cygan A, Price AA, Yost SA, Bohannon CD, Jacob J, Grakoui A, Marcotrigiano J Nature. 2014 Feb 19. doi: 10.1038/nature13117. PMID:24553139<ref>PMID:24553139</ref>	+
-		+
-	~~From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
-	~~__TOC__~~	+
-	~~</StructureSection>~~	+
-	~~[[Category: Hepatitis c virus subtype 2a]]~~	+
-	~~[[Category: Lk3 transgenic mice]]~~	+
-	~~[[Category: Khan, A G.]]~~	+
-	~~[[Category: Marcotrigiano, J.]]~~	+
-	~~[[Category: Miller, M T.]]~~	+
-	~~[[Category: Antigen]]~~	+
-	~~[[Category: Antigen recognition]]~~	+
-	~~[[Category: E2]]~~	+
-	~~[[Category: Envelope glycoprotein 2]]~~	+
-	~~[[Category: Glycosylation]]~~	+
-	~~[[Category: Hcv]]~~	+
-	~~[[Category: Hepatitis c virus]]~~	+
-	~~[[Category: Immune system-viral protein complex]]~~	+
-	~~[[Category: Mouse fab heavy chain]]~~	+
-	~~[[Category: Mouse fab light chain]]~~	+

OCA at 07:03, 28 May 2014

OCA — Wed, 28 May 2014 07:03:53 GMT

←Older revision		Revision as of 07:03, 28 May 2014
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-	~~{{STRUCTURE_4nx3\| PDB=4nx3 \| SCENE= }}~~	+	==Structure of the core ectodomain of the hepatitis C virus envelope glycoprotein 2==
-	===Structure of the core ectodomain of the hepatitis C virus envelope glycoprotein 2===	+	<StructureSection load='4nx3' size='340' side='right' caption='[[4nx3]], [[Resolution\|resolution]] 2.40Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24553139}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4nx3]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Hepatitis_c_virus_subtype_2a Hepatitis c virus subtype 2a] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NX3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NX3 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ARF:FORMAMIDE'>ARF</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nx3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nx3 RCSB], [http://www.ebi.ac.uk/pdbsum/4nx3 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Hepatitis C virus (HCV) is a significant public health concern with approximately 160 million people infected worldwide. HCV infection often results in chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. No vaccine is available and current therapies are effective against some, but not all, genotypes. HCV is an enveloped virus with two surface glycoproteins (E1 and E2). E2 binds to the host cell through interactions with scavenger receptor class B type I (SR-BI) and CD81, and serves as a target for neutralizing antibodies. Little is known about the molecular mechanism that mediates cell entry and membrane fusion, although E2 is predicted to be a class II viral fusion protein. Here we describe the structure of the E2 core domain in complex with an antigen-binding fragment (Fab) at 2.4 A resolution. The E2 core has a compact, globular domain structure, consisting mostly of beta-strands and random coil with two small alpha-helices. The strands are arranged in two, perpendicular sheets (A and B), which are held together by an extensive hydrophobic core and disulphide bonds. Sheet A has an IgG-like fold that is commonly found in viral and cellular proteins, whereas sheet B represents a novel fold. Solution-based studies demonstrate that the full-length E2 ectodomain has a similar globular architecture and does not undergo significant conformational or oligomeric rearrangements on exposure to low pH. Thus, the IgG-like fold is the only feature that E2 shares with class II membrane fusion proteins. These results provide unprecedented insights into HCV entry and will assist in developing an HCV vaccine and new inhibitors.

-	~~==About this~~ Structure==	+	Structure of the core ectodomain of the hepatitis C virus envelope glycoprotein 2.,Khan AG, Whidby J, Miller MT, Scarborough H, Zatorski AV, Cygan A, Price AA, Yost SA, Bohannon CD, Jacob J, Grakoui A, Marcotrigiano J Nature. 2014 Feb 19. doi: 10.1038/nature13117. PMID:24553139<ref>PMID:24553139</ref>
-	~~[[4nx3]] is a 3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Hepatitis_c_virus_subtype_2a Hepatitis c virus subtype 2a] and [http~~:~~//en~~.~~wikipedia.org~~/~~wiki/Lk3_transgenic_mice Lk3 transgenic mice]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4NX3 OCA].~~	+

-	==~~Reference~~==	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024553139</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Hepatitis c virus subtype 2a]]		[[Category: Hepatitis c virus subtype 2a]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]

OCA at 06:19, 23 April 2014

OCA — Wed, 23 Apr 2014 06:19:55 GMT

←Older revision		Revision as of 06:19, 23 April 2014
Line 4:		Line 4:

	==About this Structure==		==About this Structure==
-	[[4nx3]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NX3 OCA].	+	[[4nx3]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Hepatitis_c_virus_subtype_2a Hepatitis c virus subtype 2a] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NX3 OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:024553139</ref><references group="xtra"/><references/>		<ref group="xtra">PMID:024553139</ref><references group="xtra"/><references/>
		+	[[Category: Hepatitis c virus subtype 2a]]
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Khan, A G.]]		[[Category: Khan, A G.]]
	[[Category: Marcotrigiano, J.]]		[[Category: Marcotrigiano, J.]]

OCA at 08:54, 19 March 2014

OCA — Wed, 19 Mar 2014 08:54:05 GMT

←Older revision		Revision as of 08:54, 19 March 2014
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	{{STRUCTURE_4nx3\| PDB=4nx3 \| SCENE= }}		{{STRUCTURE_4nx3\| PDB=4nx3 \| SCENE= }}
	===Structure of the core ectodomain of the hepatitis C virus envelope glycoprotein 2===		===Structure of the core ectodomain of the hepatitis C virus envelope glycoprotein 2===
		+	{{ABSTRACT_PUBMED_24553139}}

	==About this Structure==		==About this Structure==
	[[4nx3]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NX3 OCA].		[[4nx3]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NX3 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:024553139</ref><references group="xtra"/><references/>
	[[Category: Khan, A G.]]		[[Category: Khan, A G.]]
	[[Category: Marcotrigiano, J.]]		[[Category: Marcotrigiano, J.]]

OCA at 18:27, 3 March 2014

OCA — Mon, 03 Mar 2014 18:27:18 GMT

←Older revision		Revision as of 18:27, 3 March 2014
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4nx3\| PDB=4nx3 \| SCENE= }}
		+	===Structure of the core ectodomain of the hepatitis C virus envelope glycoprotein 2===

-	~~The entry~~ 4nx3 is ~~ON HOLD~~	+	==About this Structure==
-		+	[[4nx3]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NX3 OCA].
-	~~Authors~~: Khan, A.G., Miller, M.T.~~, Marcotrigiano, J.~~	+	[[Category: Khan, A G.]]
-		+	[[Category: Marcotrigiano, J.]]
-	~~Description~~: ~~Mouse Fab bound to~~ virus protein	+	[[Category: Miller, M T.]]
		+	[[Category: Antigen]]
		+	[[Category: Antigen recognition]]
		+	[[Category: E2]]
		+	[[Category: Envelope glycoprotein 2]]
		+	[[Category: Glycosylation]]
		+	[[Category: Hcv]]
		+	[[Category: Hepatitis c virus]]
		+	[[Category: Immune system-viral protein complex]]
		+	[[Category: Mouse fab heavy chain]]
		+	[[Category: Mouse fab light chain]]

OCA at 07:38, 15 January 2014

OCA — Wed, 15 Jan 2014 07:38:29 GMT

←Older revision		Revision as of 07:38, 15 January 2014
Line 3:		Line 3:
	The entry 4nx3 is ON HOLD		The entry 4nx3 is ON HOLD

-	Authors: Khan, A. G., Miller, M. T., Marcotrigiano, J.	+	Authors: Khan, A.G., Miller, M.T., Marcotrigiano, J.

	Description: Mouse Fab bound to virus protein		Description: Mouse Fab bound to virus protein

OCA: Protected "4nx3" [edit=sysop:move=sysop]

OCA — Wed, 18 Dec 2013 09:25:07 GMT

Protected "4nx3" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:25, 18 December 2013

OCA: New page: '''Unreleased structure''' The entry 4nx3 is ON HOLD Authors: Khan, A. G., Miller, M. T., Marcotrigiano, J. Description: Mouse Fab bound to virus protein

OCA — Wed, 18 Dec 2013 09:25:06 GMT

New page: '''Unreleased structure''' The entry 4nx3 is ON HOLD Authors: Khan, A. G., Miller, M. T., Marcotrigiano, J. Description: Mouse Fab bound to virus protein

New page

'''Unreleased structure'''

The entry 4nx3 is ON HOLD

Authors: Khan, A. G., Miller, M. T., Marcotrigiano, J.

Description: Mouse Fab bound to virus protein