4o1r - Revision history

OCA at 17:05, 20 September 2023

OCA — Wed, 20 Sep 2023 17:05:57 GMT

←Older revision		Revision as of 17:05, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4o1r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_punctiforme_PCC_73102 Nostoc punctiforme PCC 73102]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O1R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O1R FirstGlance]. <br>		<table><tr><td colspan='2'>[[4o1r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_punctiforme_PCC_73102 Nostoc punctiforme PCC 73102]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O1R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O1R FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o1r OCA], [https://pdbe.org/4o1r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o1r RCSB], [https://www.ebi.ac.uk/pdbsum/4o1r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o1r ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o1r OCA], [https://pdbe.org/4o1r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o1r RCSB], [https://www.ebi.ac.uk/pdbsum/4o1r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o1r ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:52, 18 January 2023

OCA — Wed, 18 Jan 2023 08:52:09 GMT

←Older revision		Revision as of 08:52, 18 January 2023
Line 3:		Line 3:
	<StructureSection load='4o1r' size='340' side='right'caption='[[4o1r]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='4o1r' size='340' side='right'caption='[[4o1r]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4o1r]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Nosp7 Nosp7~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O1R OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4O1R FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4o1r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_punctiforme_PCC_73102 Nostoc punctiforme PCC 73102]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O1R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O1R FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Npun_F2831 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63737 NOSP7])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o1r OCA], [https://pdbe.org/4o1r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o1r RCSB], [https://www.ebi.ac.uk/pdbsum/4o1r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o1r ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4o1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o1r OCA], [~~http~~://pdbe.org/4o1r PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4o1r RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4o1r PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4o1r ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B2IVH9_NOSP7 B2IVH9_NOSP7]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Nosp7~~]]	+	[[Category: Nostoc punctiforme PCC 73102]]
-	[[Category: Aranko~~, A S~~]]	+	[[Category: Aranko AS]]
-	[[Category: Iwai, H]]	+	[[Category: Iwai H]]
-	[[Category: Kajander, T]]	+	[[Category: Kajander T]]
-	[[Category: Oeemig~~, J S~~]]	+	[[Category: Oeemig JS]]
-	[[Category: Wlodawer, A]]	+	[[Category: Wlodawer A]]
-	[[Category: Zhou, D]]	+	[[Category: Zhou D]]
-	~~[[Category: Splicing~~]]	+

OCA at 12:12, 17 July 2019

OCA — Wed, 17 Jul 2019 12:12:05 GMT

←Older revision		Revision as of 12:12, 17 July 2019
Line 1:		Line 1:

	==Crystal structure of NpuDnaB intein==		==Crystal structure of NpuDnaB intein==
-	<StructureSection load='4o1r' size='340' side='right' caption='[[4o1r]], [[Resolution\|resolution]] 1.40Å' scene=''>	+	<StructureSection load='4o1r' size='340' side='right'caption='[[4o1r]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4o1r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nosp7 Nosp7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O1R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O1R FirstGlance]. <br>		<table><tr><td colspan='2'>[[4o1r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nosp7 Nosp7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O1R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O1R FirstGlance]. <br>
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Nosp7]]		[[Category: Nosp7]]
	[[Category: Aranko, A S]]		[[Category: Aranko, A S]]

OCA at 14:43, 11 August 2016

OCA — Thu, 11 Aug 2016 14:43:56 GMT

←Older revision		Revision as of 14:43, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of NpuDnaB intein==		==Crystal structure of NpuDnaB intein==
	<StructureSection load='4o1r' size='340' side='right' caption='[[4o1r]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='4o1r' size='340' side='right' caption='[[4o1r]], [[Resolution\|resolution]] 1.40Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Npun_F2831 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63737 NOSP7])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Npun_F2831 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63737 NOSP7])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o1r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4o1r RCSB], [http://www.ebi.ac.uk/pdbsum/4o1r PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o1r OCA], [http://pdbe.org/4o1r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o1r RCSB], [http://www.ebi.ac.uk/pdbsum/4o1r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4o1r ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4o1r" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:59, 5 January 2015

OCA — Mon, 05 Jan 2015 08:59:21 GMT

←Older revision		Revision as of 08:59, 5 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_4o1r\| PDB~~=4o1r \| ~~SCENE~~= }}	+	==Crystal structure of NpuDnaB intein==
-	===~~Crystal~~ structure ~~of NpuDnaB intein~~===	+	<StructureSection load='4o1r' size='340' side='right' caption='[[4o1r]], [[Resolution\|resolution]] 1.40Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24574026}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4o1r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nosp7 Nosp7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O1R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O1R FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Npun_F2831 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63737 NOSP7])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o1r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4o1r RCSB], [http://www.ebi.ac.uk/pdbsum/4o1r PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Protein splicing is an autocatalytic process involving self-excision of an internal protein domain, the intein, and concomitant ligation of the two flanking sequences, the exteins, with a peptide bond. Protein splicing can also take place in trans by naturally split inteins or artificially split inteins, ligating the exteins on two different polypeptide chains into one polypeptide chain. Protein trans-splicing could work in foreign contexts by replacing the native extein sequences with other protein sequences. Protein ligation using protein trans-splicing increasingly becomes a useful tool for biotechnological applications such as semi-synthesis of proteins, segmental isotopic labeling, and in vivo protein engineering. However, only a few split inteins have been successfully applied for protein ligation. Naturally split inteins have been widely used, but they are cross-reactive to each other, limiting their applications to multiple-fragment ligation. Based on the three-dimensional structures including two newly determined intein structures, we derived 21 new split inteins from four highly efficient cis-splicing inteins, in order to develop novel split inteins suitable for protein ligation. We systematically compared trans-splicing of 24 split inteins and tested the cross-activities among them to identify orthogonal split intein fragments that could be used in chemical biology and biotechnological applications.

-	~~==About this~~ Structure==	+	Structure-based engineering and comparison of novel split inteins for protein ligation.,Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H Mol Biosyst. 2014 Feb 26. PMID:24574026<ref>PMID:24574026</ref>
-	~~[[4o1r]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Nosp7 Nosp7]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4O1R OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024574026</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Nosp7]]		[[Category: Nosp7]]
-	[[Category: Aranko, A S.]]	+	[[Category: Aranko, A S]]
-	[[Category: Iwai, H.]]	+	[[Category: Iwai, H]]
-	[[Category: Kajander, T.]]	+	[[Category: Kajander, T]]
-	[[Category: Oeemig, J S.]]	+	[[Category: Oeemig, J S]]
-	[[Category: Wlodawer, A.]]	+	[[Category: Wlodawer, A]]
-	[[Category: Zhou, D.]]	+	[[Category: Zhou, D]]
	[[Category: Splicing]]		[[Category: Splicing]]

OCA at 07:46, 16 April 2014

OCA — Wed, 16 Apr 2014 07:46:17 GMT

←Older revision		Revision as of 07:46, 16 April 2014
Line 4:		Line 4:

	==About this Structure==		==About this Structure==
-	[[4o1r]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O1R OCA].	+	[[4o1r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nosp7 Nosp7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O1R OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:024574026</ref><references group="xtra"/><references/>		<ref group="xtra">PMID:024574026</ref><references group="xtra"/><references/>
		+	[[Category: Nosp7]]
	[[Category: Aranko, A S.]]		[[Category: Aranko, A S.]]
	[[Category: Iwai, H.]]		[[Category: Iwai, H.]]

OCA at 09:08, 19 March 2014

OCA — Wed, 19 Mar 2014 09:08:34 GMT

←Older revision		Revision as of 09:08, 19 March 2014
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4o1r\| PDB=4o1r \| SCENE= }}
		+	===Crystal structure of NpuDnaB intein===
		+	{{ABSTRACT_PUBMED_24574026}}

-	~~The entry~~ 4o1r is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
		+	[[4o1r]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O1R OCA].

-	~~Authors~~: Aranko, A.S., ~~Oeemig, J.S~~.~~, Zhou, D.,~~ Kajander, T., Wlodawer, A., ~~Iwai, H~~.	+	==Reference==
-		+	<ref group="xtra">PMID:024574026</ref><references group="xtra"/><references/>
-	~~Description~~: ~~Crystal structure of a intein NpuDnaB~~	+	[[Category: Aranko, A S.]]
		+	[[Category: Iwai, H.]]
		+	[[Category: Kajander, T.]]
		+	[[Category: Oeemig, J S.]]
		+	[[Category: Wlodawer, A.]]
		+	[[Category: Zhou, D.]]
		+	[[Category: Splicing]]

OCA at 09:22, 8 January 2014

OCA — Wed, 08 Jan 2014 09:22:07 GMT

←Older revision		Revision as of 09:22, 8 January 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4o1r is ON HOLD	+	The entry 4o1r is ON HOLD until Paper Publication

-	Authors: Aranko, A.S., Oeemig, J.S., Zhou, D, Kajander, T, Wlodawer, A, Iwai, H	+	Authors: Aranko, A.S., Oeemig, J.S., Zhou, D., Kajander, T., Wlodawer, A., Iwai, H.

	Description: Crystal structure of a intein NpuDnaB		Description: Crystal structure of a intein NpuDnaB

OCA: Protected "4o1r" [edit=sysop:move=sysop]

OCA — Wed, 25 Dec 2013 09:30:36 GMT

Protected "4o1r" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:30, 25 December 2013

OCA: New page: '''Unreleased structure''' The entry 4o1r is ON HOLD Authors: Aranko, A.S., Oeemig, J.S., Zhou, D, Kajander, T, Wlodawer, A, Iwai, H Description: Crystal structure of a intein NpuDnaB

OCA — Wed, 25 Dec 2013 09:30:35 GMT

New page: '''Unreleased structure''' The entry 4o1r is ON HOLD Authors: Aranko, A.S., Oeemig, J.S., Zhou, D, Kajander, T, Wlodawer, A, Iwai, H Description: Crystal structure of a intein NpuDnaB

New page

'''Unreleased structure'''

The entry 4o1r is ON HOLD

Authors: Aranko, A.S., Oeemig, J.S., Zhou, D, Kajander, T, Wlodawer, A, Iwai, H

Description: Crystal structure of a intein NpuDnaB