4o6m - Revision history

OCA at 12:38, 1 March 2024

OCA — Fri, 01 Mar 2024 12:38:04 GMT

←Older revision		Revision as of 12:38, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4o6m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O6M FirstGlance]. <br>		<table><tr><td colspan='2'>[[4o6m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O6M FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPG:[(Z)-OCTADEC-9-ENYL]+(2R)-2,3-BIS(OXIDANYL)PROPANOATE'>MPG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.901Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPG:[(Z)-OCTADEC-9-ENYL]+(2R)-2,3-BIS(OXIDANYL)PROPANOATE'>MPG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o6m OCA], [https://pdbe.org/4o6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o6m RCSB], [https://www.ebi.ac.uk/pdbsum/4o6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o6m ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o6m OCA], [https://pdbe.org/4o6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o6m RCSB], [https://www.ebi.ac.uk/pdbsum/4o6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o6m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/O27985_ARCFU O27985_ARCFU]		[https://www.uniprot.org/uniprot/O27985_ARCFU O27985_ARCFU]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The CDP-alcohol phosphotransferase (CDP-AP) family of integral membrane enzymes catalyses the transfer of a substituted phosphate group from a CDP-linked donor to an alcohol acceptor. This is an essential reaction for phospholipid biosynthesis across all kingdoms of life, and it is catalysed solely by CDP-APs. Here we report the 2.0 A resolution crystal structure of a representative CDP-AP from Archaeoglobus fulgidus. The enzyme (AF2299) is a homodimer, with each protomer consisting of six transmembrane helices and an N-terminal cytosolic domain. A polar cavity within the membrane accommodates the active site, lined with the residues from an absolutely conserved CDP-AP signature motif (D(1)xxD(2)G(1)xxAR...G(2)xxxD(3)xxxD(4)). Structures in the apo, CMP-bound, CDP-bound and CDP-glycerol-bound states define functional roles for each of these eight conserved residues and allow us to propose a sequential, base-catalysed mechanism universal for CDP-APs, in which the fourth aspartate (D4) acts as the catalytic base.
-
-	Structural basis for catalysis in a CDP-alcohol phosphotransferase.,Sciara G, Clarke OB, Tomasek D, Kloss B, Tabuso S, Byfield R, Cohn R, Banerjee S, Rajashankar KR, Slavkovic V, Graziano JH, Shapiro L, Mancia F Nat Commun. 2014 Jun 13;5:4068. doi: 10.1038/ncomms5068. PMID:24923293<ref>PMID:24923293</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4o6m" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Phosphotransferase 3D structures\|Phosphotransferase 3D structures]]		*[[Phosphotransferase 3D structures\|Phosphotransferase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:58, 18 January 2023

OCA — Wed, 18 Jan 2023 08:58:16 GMT

←Older revision		Revision as of 08:58, 18 January 2023
Line 3:		Line 3:
	<StructureSection load='4o6m' size='340' side='right'caption='[[4o6m]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4o6m' size='340' side='right'caption='[[4o6m]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4o6m]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Arcfu Arcfu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O6M OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4O6M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4o6m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O6M FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPG:[(Z)-OCTADEC-9-ENYL]+(2R)-2,3-BIS(OXIDANYL)PROPANOATE'>MPG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPG:[(Z)-OCTADEC-9-ENYL]+(2R)-2,3-BIS(OXIDANYL)PROPANOATE'>MPG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4o6n\|4o6n]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o6m OCA], [https://pdbe.org/4o6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o6m RCSB], [https://www.ebi.ac.uk/pdbsum/4o6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o6m ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AF2299, AF_2299 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224325 ARCFU])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4o6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o6m OCA], [~~http~~://pdbe.org/4o6m PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4o6m RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4o6m PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4o6m ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O27985_ARCFU O27985_ARCFU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[Phosphotransferase\|Phosphotransferase]]	+	*[[Phosphotransferase 3D structures\|Phosphotransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arcfu~~]]	+	[[Category: Archaeoglobus fulgidus DSM 4304]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Banerjee, S]]	+	[[Category: Banerjee S]]
-	[[Category: Clarke~~, O B~~]]	+	[[Category: Clarke OB]]
-	[[Category: Mancia, F]]	+	[[Category: Mancia F]]
-	~~[[Category: NYCOMPS, New York Consortium on Membrane Protein Structure~~]]	+	[[Category: Rajashankar KR]]
-	[[Category: Rajashankar~~, K R~~]]	+	[[Category: Sciara G]]
-	[[Category: Sciara, G]]	+	[[Category: Shapiro L]]
-	[[Category: Shapiro, L]]	+	[[Category: Tomasek D]]
-	[[Category: Tomasek, D]]	+
-	~~[[Category: Cdp-alcohol phosphotransferase]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: New york consortium on membrane protein structure]]~~	+
-	~~[[Category: Nycomp]]~~	+
-	~~[[Category: Psi-biology]]~~	+
-	~~[[Category: Structural genomic]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 08:37, 10 April 2019

OCA — Wed, 10 Apr 2019 08:37:26 GMT

←Older revision		Revision as of 08:37, 10 April 2019
Line 1:		Line 1:

	==Structure of AF2299, a CDP-alcohol phosphotransferase (CMP-bound)==		==Structure of AF2299, a CDP-alcohol phosphotransferase (CMP-bound)==
-	<StructureSection load='4o6m' size='340' side='right' caption='[[4o6m]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='4o6m' size='340' side='right'caption='[[4o6m]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4o6m]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O6M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O6M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4o6m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arcfu Arcfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O6M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O6M FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPG:[(Z)-OCTADEC-9-ENYL]+(2R)-2,3-BIS(OXIDANYL)PROPANOATE'>MPG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPG:[(Z)-OCTADEC-9-ENYL]+(2R)-2,3-BIS(OXIDANYL)PROPANOATE'>MPG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4o6n\|4o6n]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4o6n\|4o6n]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AF2299, AF_2299 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224325 ARCFU])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o6m OCA], [http://pdbe.org/4o6m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o6m RCSB], [http://www.ebi.ac.uk/pdbsum/4o6m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4o6m ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o6m OCA], [http://pdbe.org/4o6m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o6m RCSB], [http://www.ebi.ac.uk/pdbsum/4o6m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4o6m ProSAT]</span></td></tr>
	</table>		</table>
Line 17:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 4o6m" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4o6m" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Phosphotransferase\|Phosphotransferase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arcfu]]
		+	[[Category: Large Structures]]
	[[Category: Banerjee, S]]		[[Category: Banerjee, S]]
	[[Category: Clarke, O B]]		[[Category: Clarke, O B]]

OCA at 19:04, 11 August 2016

OCA — Thu, 11 Aug 2016 19:04:12 GMT

←Older revision		Revision as of 19:04, 11 August 2016
Line 1:		Line 1:
		+
	==Structure of AF2299, a CDP-alcohol phosphotransferase (CMP-bound)==		==Structure of AF2299, a CDP-alcohol phosphotransferase (CMP-bound)==
	<StructureSection load='4o6m' size='340' side='right' caption='[[4o6m]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4o6m' size='340' side='right' caption='[[4o6m]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4o6m]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O6M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O6M FirstGlance]. <br>		<table><tr><td colspan='2'>[[4o6m]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O6M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O6M FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPG:1-~~MONOOLEOYL~~-~~RAC~~-~~GLYCEROL~~'>MPG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPG:[(Z)-OCTADEC-9-ENYL]+(2R)-2,3-BIS(OXIDANYL)PROPANOATE'>MPG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4o6n\|4o6n]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4o6n\|4o6n]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o6m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4o6m RCSB], [http://www.ebi.ac.uk/pdbsum/4o6m PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o6m OCA], [http://pdbe.org/4o6m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o6m RCSB], [http://www.ebi.ac.uk/pdbsum/4o6m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4o6m ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4o6m" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:37, 5 January 2015

OCA — Mon, 05 Jan 2015 14:37:31 GMT

←Older revision		Revision as of 14:37, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4o6m]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O6M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O6M FirstGlance]. <br>		<table><tr><td colspan='2'>[[4o6m]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O6M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O6M FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPG:1-MONOOLEOYL-RAC-GLYCEROL'>MPG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPG:1-MONOOLEOYL-RAC-GLYCEROL'>MPG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4o6n\|4o6n]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4o6n\|4o6n]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o6m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4o6m RCSB], [http://www.ebi.ac.uk/pdbsum/4o6m PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o6m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4o6m RCSB], [http://www.ebi.ac.uk/pdbsum/4o6m PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Banerjee, S.]]	+	[[Category: Banerjee, S]]
-	[[Category: Clarke, O B.]]	+	[[Category: Clarke, O B]]
-	[[Category: Mancia, F.]]	+	[[Category: Mancia, F]]
-	[[Category: NYCOMPS, New York Consortium on Membrane Protein Structure.]]	+	[[Category: NYCOMPS, New York Consortium on Membrane Protein Structure]]
-	[[Category: Rajashankar, K R.]]	+	[[Category: Rajashankar, K R]]
-	[[Category: Sciara, G.]]	+	[[Category: Sciara, G]]
-	[[Category: Shapiro, L.]]	+	[[Category: Shapiro, L]]
-	[[Category: Tomasek, D.]]	+	[[Category: Tomasek, D]]
	[[Category: Cdp-alcohol phosphotransferase]]		[[Category: Cdp-alcohol phosphotransferase]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]

OCA at 06:52, 13 August 2014

OCA — Wed, 13 Aug 2014 06:52:09 GMT

←Older revision		Revision as of 06:52, 13 August 2014
Line 7:		Line 7:
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o6m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4o6m RCSB], [http://www.ebi.ac.uk/pdbsum/4o6m PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o6m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4o6m RCSB], [http://www.ebi.ac.uk/pdbsum/4o6m PDBsum]</span></td></tr>
	<table>		<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The CDP-alcohol phosphotransferase (CDP-AP) family of integral membrane enzymes catalyses the transfer of a substituted phosphate group from a CDP-linked donor to an alcohol acceptor. This is an essential reaction for phospholipid biosynthesis across all kingdoms of life, and it is catalysed solely by CDP-APs. Here we report the 2.0 A resolution crystal structure of a representative CDP-AP from Archaeoglobus fulgidus. The enzyme (AF2299) is a homodimer, with each protomer consisting of six transmembrane helices and an N-terminal cytosolic domain. A polar cavity within the membrane accommodates the active site, lined with the residues from an absolutely conserved CDP-AP signature motif (D(1)xxD(2)G(1)xxAR...G(2)xxxD(3)xxxD(4)). Structures in the apo, CMP-bound, CDP-bound and CDP-glycerol-bound states define functional roles for each of these eight conserved residues and allow us to propose a sequential, base-catalysed mechanism universal for CDP-APs, in which the fourth aspartate (D4) acts as the catalytic base.
		+
		+	Structural basis for catalysis in a CDP-alcohol phosphotransferase.,Sciara G, Clarke OB, Tomasek D, Kloss B, Tabuso S, Byfield R, Cohn R, Banerjee S, Rajashankar KR, Slavkovic V, Graziano JH, Shapiro L, Mancia F Nat Commun. 2014 Jun 13;5:4068. doi: 10.1038/ncomms5068. PMID:24923293<ref>PMID:24923293</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:07, 21 May 2014

OCA — Wed, 21 May 2014 07:07:55 GMT

←Older revision		Revision as of 07:07, 21 May 2014
Line 1:		Line 1:
-	==Structure of a ~~Membrane Protein~~==	+	==Structure of AF2299, a CDP-alcohol phosphotransferase (CMP-bound)==
	<StructureSection load='4o6m' size='340' side='right' caption='[[4o6m]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4o6m' size='340' side='right' caption='[[4o6m]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4o6m]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O6M OCA]. <br>	+	<table><tr><td colspan='2'>[[4o6m]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O6M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O6M FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPG:1-MONOOLEOYL-RAC-GLYCEROL'>MPG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>		</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPG:1-MONOOLEOYL-RAC-GLYCEROL'>MPG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4o6n\|4o6n]]</td></tr>		<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4o6n\|4o6n]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o6m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4o6m RCSB], [http://www.ebi.ac.uk/pdbsum/4o6m PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o6m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4o6m RCSB], [http://www.ebi.ac.uk/pdbsum/4o6m PDBsum]</span></td></tr>
	<table>		<table>

OCA at 07:26, 14 May 2014

OCA — Wed, 14 May 2014 07:26:53 GMT

←Older revision		Revision as of 07:26, 14 May 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Structure of a Membrane Protein==
-		+	<StructureSection load='4o6m' size='340' side='right' caption='[[4o6m]], [[Resolution\|resolution]] 1.90Å' scene=''>
-	~~The entry~~ 4o6m is ~~ON HOLD~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4o6m]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O6M OCA]. <br>
-	~~Authors~~: ~~Clarke~~, O.B., ~~Sciara, G~~., ~~Tomasek, D~~., ~~Banerjee, S~~., ~~Rajashankar, K~~.R., ~~Shapiro, L~~., Mancia, F., New York Consortium on Membrane Protein Structure ~~(NYCOMPS)~~	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPG:1-MONOOLEOYL-RAC-GLYCEROL'>MPG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
-		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4o6n\|4o6n]]</td></tr>
-	~~Description~~: ~~Structure of a~~ Membrane ~~Protein~~	+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o6m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4o6m RCSB], [http://www.ebi.ac.uk/pdbsum/4o6m PDBsum]</span></td></tr>
		+	<table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Banerjee, S.]]
		+	[[Category: Clarke, O B.]]
		+	[[Category: Mancia, F.]]
		+	[[Category: NYCOMPS, New York Consortium on Membrane Protein Structure.]]
		+	[[Category: Rajashankar, K R.]]
		+	[[Category: Sciara, G.]]
		+	[[Category: Shapiro, L.]]
		+	[[Category: Tomasek, D.]]
		+	[[Category: Cdp-alcohol phosphotransferase]]
		+	[[Category: Membrane protein]]
		+	[[Category: New york consortium on membrane protein structure]]
		+	[[Category: Nycomp]]
		+	[[Category: Psi-biology]]
		+	[[Category: Structural genomic]]
		+	[[Category: Transferase]]

OCA: Protected "4o6m" [edit=sysop:move=sysop]

OCA — Wed, 08 Jan 2014 09:24:51 GMT

Protected "4o6m" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:24, 8 January 2014

OCA: New page: '''Unreleased structure''' The entry 4o6m is ON HOLD Authors: Clarke, O.B., Sciara, G., Tomasek, D., Banerjee, S., Rajashankar, K.R., Shapiro, L., Mancia, F., New York Consortium on Mem...

OCA — Wed, 08 Jan 2014 09:24:49 GMT

New page: '''Unreleased structure''' The entry 4o6m is ON HOLD Authors: Clarke, O.B., Sciara, G., Tomasek, D., Banerjee, S., Rajashankar, K.R., Shapiro, L., Mancia, F., New York Consortium on Mem...

New page

'''Unreleased structure'''

The entry 4o6m is ON HOLD

Authors: Clarke, O.B., Sciara, G., Tomasek, D., Banerjee, S., Rajashankar, K.R., Shapiro, L., Mancia, F., New York Consortium on Membrane Protein Structure (NYCOMPS)

Description: Structure of a Membrane Protein