4oaq - Revision history

OCA at 17:09, 20 September 2023

OCA — Wed, 20 Sep 2023 17:09:01 GMT

←Older revision		Revision as of 17:09, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4oaq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_parapsilosis Candida parapsilosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OAQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OAQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4oaq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_parapsilosis Candida parapsilosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OAQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OAQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.858Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oaq OCA], [https://pdbe.org/4oaq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oaq RCSB], [https://www.ebi.ac.uk/pdbsum/4oaq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oaq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oaq OCA], [https://pdbe.org/4oaq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oaq RCSB], [https://www.ebi.ac.uk/pdbsum/4oaq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oaq ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:14, 25 January 2023

OCA — Wed, 25 Jan 2023 07:14:08 GMT

←Older revision		Revision as of 07:14, 25 January 2023
Line 3:		Line 3:
	<StructureSection load='4oaq' size='340' side='right'caption='[[4oaq]], [[Resolution\|resolution]] 1.86Å' scene=''>		<StructureSection load='4oaq' size='340' side='right'caption='[[4oaq]], [[Resolution\|resolution]] 1.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4oaq]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_22019 Atcc 22019~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OAQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4OAQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4oaq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_parapsilosis Candida parapsilosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OAQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OAQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CpCR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5480 ATCC 22019])</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oaq OCA], [https://pdbe.org/4oaq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oaq RCSB], [https://www.ebi.ac.uk/pdbsum/4oaq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oaq ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cinnamyl-alcohol_dehydrogenase Cinnamyl-alcohol dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.195 1.1.1.195] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4oaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oaq OCA], [~~http~~://pdbe.org/4oaq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4oaq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4oaq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4oaq ProSAT]</span></td></tr>	+
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/M4VRJ6_CANPA M4VRJ6_CANPA]
-	The NAD(P)H-dependent carbonyl reductase from Candida parapsilosis ATCC 7330 catalyses the asymmetric reduction of ethyl 4-phenyl-2-oxobutanoate to ethyl (R)-4-phenyl-2-hydroxybutanoate, a precursor of angiotensin-converting enzyme inhibitors such as Cilazapril and Benazepril. The carbonyl reductase was expressed in Escherichia coli and purified by GST-affinity and size-exclusion chromatography. Crystals were obtained by the hanging-drop vapour-diffusion method and diffracted to 1.86 A resolution. The asymmetric unit contained two molecules of carbonyl reductase, with a solvent content of 48%. The structure was solved by molecular replacement using cinnamyl alcohol dehydrogenase from Saccharomyces cerevisiae as a search model.	+
-		+
-	Expression, purification, crystallization and preliminary X-ray diffraction analysis of carbonyl reductase from Candida parapsilosis ATCC 7330.,Aggarwal N, Mandal PK, Gautham N, Chadha A Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Mar 1;69(Pt 3):~~313-5. doi:, 10.1107~~/~~S1744309113003667. Epub 2013 Feb 27. PMID:23519811<ref>PMID:23519811<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 4oaq" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
-	*[[Carbonyl reductase\|Carbonyl reductase]]	+	*[[Carbonyl reductase 3D structures\|Carbonyl reductase 3D structures]]
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 22019]]~~	+	[[Category: Candida parapsilosis]]
-	~~[[Category: Cinnamyl-alcohol dehydrogenase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Aggrawal, N]]	+	[[Category: Aggrawal N]]
-	[[Category: Chadha, A]]	+	[[Category: Chadha A]]
-	[[Category: Gautham, N]]	+	[[Category: Gautham N]]
-	[[Category: Mandal~~, P K]]~~	+	[[Category: Mandal PK]]
-	~~[[Category: Nadph]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Rossmann fold]]~~	+
-	~~[[Category: Stereoselectivity]]~~	+
-	~~[[Category: Zinc dependent carbonyl reductase~~]]	+

OCA at 08:52, 10 April 2019

OCA — Wed, 10 Apr 2019 08:52:32 GMT

←Older revision		Revision as of 08:52, 10 April 2019
Line 1:		Line 1:

	==Crystal structure of the R-specific Carbonyl Reductase from Candida parapsilosis ATCC 7330==		==Crystal structure of the R-specific Carbonyl Reductase from Candida parapsilosis ATCC 7330==
-	<StructureSection load='4oaq' size='340' side='right' caption='[[4oaq]], [[Resolution\|resolution]] 1.86Å' scene=''>	+	<StructureSection load='4oaq' size='340' side='right'caption='[[4oaq]], [[Resolution\|resolution]] 1.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4oaq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OAQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OAQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4oaq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_22019 Atcc 22019]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OAQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OAQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CpCR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5480 ATCC 22019])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cinnamyl-alcohol_dehydrogenase Cinnamyl-alcohol dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.195 1.1.1.195] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cinnamyl-alcohol_dehydrogenase Cinnamyl-alcohol dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.195 1.1.1.195] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oaq OCA], [http://pdbe.org/4oaq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4oaq RCSB], [http://www.ebi.ac.uk/pdbsum/4oaq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4oaq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oaq OCA], [http://pdbe.org/4oaq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4oaq RCSB], [http://www.ebi.ac.uk/pdbsum/4oaq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4oaq ProSAT]</span></td></tr>
Line 24:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 22019]]
	[[Category: Cinnamyl-alcohol dehydrogenase]]		[[Category: Cinnamyl-alcohol dehydrogenase]]
		+	[[Category: Large Structures]]
	[[Category: Aggrawal, N]]		[[Category: Aggrawal, N]]
	[[Category: Chadha, A]]		[[Category: Chadha, A]]

OCA at 16:59, 2 January 2017

OCA — Mon, 02 Jan 2017 16:59:12 GMT

←Older revision		Revision as of 16:59, 2 January 2017
Line 1:		Line 1:
		+
	==Crystal structure of the R-specific Carbonyl Reductase from Candida parapsilosis ATCC 7330==		==Crystal structure of the R-specific Carbonyl Reductase from Candida parapsilosis ATCC 7330==
	<StructureSection load='4oaq' size='340' side='right' caption='[[4oaq]], [[Resolution\|resolution]] 1.86Å' scene=''>		<StructureSection load='4oaq' size='340' side='right' caption='[[4oaq]], [[Resolution\|resolution]] 1.86Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cinnamyl-alcohol_dehydrogenase Cinnamyl-alcohol dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.195 1.1.1.195] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cinnamyl-alcohol_dehydrogenase Cinnamyl-alcohol dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.195 1.1.1.195] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oaq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4oaq RCSB], [http://www.ebi.ac.uk/pdbsum/4oaq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oaq OCA], [http://pdbe.org/4oaq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4oaq RCSB], [http://www.ebi.ac.uk/pdbsum/4oaq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4oaq ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4oaq" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Carbonyl reductase\|Carbonyl reductase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:54, 12 February 2015

OCA — Thu, 12 Feb 2015 11:54:34 GMT

←Older revision		Revision as of 11:54, 12 February 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of the R-specific Carbonyl Reductase from Candida parapsilosis ATCC 7330==
		+	<StructureSection load='4oaq' size='340' side='right' caption='[[4oaq]], [[Resolution\|resolution]] 1.86Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4oaq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OAQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OAQ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cinnamyl-alcohol_dehydrogenase Cinnamyl-alcohol dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.195 1.1.1.195] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oaq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4oaq RCSB], [http://www.ebi.ac.uk/pdbsum/4oaq PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The NAD(P)H-dependent carbonyl reductase from Candida parapsilosis ATCC 7330 catalyses the asymmetric reduction of ethyl 4-phenyl-2-oxobutanoate to ethyl (R)-4-phenyl-2-hydroxybutanoate, a precursor of angiotensin-converting enzyme inhibitors such as Cilazapril and Benazepril. The carbonyl reductase was expressed in Escherichia coli and purified by GST-affinity and size-exclusion chromatography. Crystals were obtained by the hanging-drop vapour-diffusion method and diffracted to 1.86 A resolution. The asymmetric unit contained two molecules of carbonyl reductase, with a solvent content of 48%. The structure was solved by molecular replacement using cinnamyl alcohol dehydrogenase from Saccharomyces cerevisiae as a search model.

-	~~The entry 4oaq is ON HOLD until Paper Publication~~	+	Expression, purification, crystallization and preliminary X-ray diffraction analysis of carbonyl reductase from Candida parapsilosis ATCC 7330.,Aggarwal N, Mandal PK, Gautham N, Chadha A Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Mar 1;69(Pt 3):313-5. doi:, 10.1107/S1744309113003667. Epub 2013 Feb 27. PMID:23519811<ref>PMID:23519811</ref>

-	~~Authors: Aggrawal~~, N.~~, Mandal, P~~.~~K., Gautham, N., Chadha, A~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Crystal structure of the R-specific Carbonyl Reductase from Candida parapsilosis ATCC 7330~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cinnamyl-alcohol dehydrogenase]]
	[[Category: Aggrawal, N]]		[[Category: Aggrawal, N]]
		+	[[Category: Chadha, A]]
	[[Category: Gautham, N]]		[[Category: Gautham, N]]
-	[[Category: Mandal, P.K]]	+	[[Category: Mandal, P K]]
-	[[Category: ~~Chadha, A~~]]	+	[[Category: Nadph]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Rossmann fold]]
		+	[[Category: Stereoselectivity]]
		+	[[Category: Zinc dependent carbonyl reductase]]

OCA at 14:21, 24 December 2014

OCA — Wed, 24 Dec 2014 14:21:12 GMT

←Older revision		Revision as of 14:21, 24 December 2014
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	Description: Crystal structure of the R-specific Carbonyl Reductase from Candida parapsilosis ATCC 7330		Description: Crystal structure of the R-specific Carbonyl Reductase from Candida parapsilosis ATCC 7330
		+	[[Category: Unreleased Structures]]
		+	[[Category: Aggrawal, N]]
		+	[[Category: Gautham, N]]
		+	[[Category: Mandal, P.K]]
		+	[[Category: Chadha, A]]

OCA at 07:50, 24 September 2014

OCA — Wed, 24 Sep 2014 07:50:51 GMT

←Older revision		Revision as of 07:50, 24 September 2014
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4oaq is ON HOLD	+	The entry 4oaq is ON HOLD until Paper Publication

	Authors: Aggrawal, N., Mandal, P.K., Gautham, N., Chadha, A.		Authors: Aggrawal, N., Mandal, P.K., Gautham, N., Chadha, A.

	Description: Crystal structure of the R-specific Carbonyl Reductase from Candida parapsilosis ATCC 7330		Description: Crystal structure of the R-specific Carbonyl Reductase from Candida parapsilosis ATCC 7330

OCA: Protected "4oaq" [edit=sysop:move=sysop]

OCA — Wed, 15 Jan 2014 07:44:16 GMT

Protected "4oaq" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:44, 15 January 2014

OCA: New page: '''Unreleased structure''' The entry 4oaq is ON HOLD Authors: Aggrawal, N., Mandal, P.K., Gautham, N., Chadha, A. Description: Crystal structure of the R-specific Carbonyl Reductase fr...

OCA — Wed, 15 Jan 2014 07:44:15 GMT

New page: '''Unreleased structure''' The entry 4oaq is ON HOLD Authors: Aggrawal, N., Mandal, P.K., Gautham, N., Chadha, A. Description: Crystal structure of the R-specific Carbonyl Reductase fr...

New page

'''Unreleased structure'''

The entry 4oaq is ON HOLD

Authors: Aggrawal, N., Mandal, P.K., Gautham, N., Chadha, A.

Description: Crystal structure of the R-specific Carbonyl Reductase from Candida parapsilosis ATCC 7330