4oca - Revision history

OCA at 12:39, 1 March 2024

OCA — Fri, 01 Mar 2024 12:39:03 GMT

←Older revision		Revision as of 12:39, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4oca]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OCA FirstGlance]. <br>		<table><tr><td colspan='2'>[[4oca]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OCA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2QR:(2R,3R,4S,5S)-3,4-dihydroxy-5-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]tetrahydro-2H-pyr+an-2-yl+[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl+dihydrogen+diphosphate'>2QR</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2QR:(2R,3R,4S,5S)-3,4-dihydroxy-5-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]tetrahydro-2H-pyr+an-2-yl+[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl+dihydrogen+diphosphate'>2QR</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oca OCA], [https://pdbe.org/4oca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oca RCSB], [https://www.ebi.ac.uk/pdbsum/4oca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oca ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oca OCA], [https://pdbe.org/4oca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oca RCSB], [https://www.ebi.ac.uk/pdbsum/4oca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oca ProSAT]</span></td></tr>
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Cationic Antimicrobial Peptides (CAMPs) represent a first line of defense against bacterial colonization. When fighting Gram-negative bacteria, CAMPs initially interact electrostatically with the negatively charged phosphate groups in lipid A and are thought to kill bacteria by disrupting their membrane integrity. However, many human pathogens, including Salmonella and Pseudomonas , have evolved lipid A modification mechanisms that result in resistance to CAMPs and related antibiotics such as Colistin. The addition of 4-amino-4-deoxy-l-Arabinose (Ara4N) to a phosphate group in lipid A is one such modification, frequently found in Pseudomonas isolated from cystic fibrosis patients. The pathway for biosynthesis of Ara4N-lipid A requires conversion of UDP-Glucuronic acid into UDP-Ara4N and subsequent transfer of the amino-sugar to lipid A. ArnB is a pyridoxal-phosphate (PLP) dependent transaminase that catalyzes a crucial step in the pathway: synthesis of UDP-Ara4N from UDP-4-keto-pentose. Here we present the 2.3 A resolution crystal structure of an active site mutant of ArnB (K188A) in complex with the reaction intermediate aldimine formed by UDP-Ara4N and PLP. The sugar-nucleotide binding site is in a cleft between the subunits of the ArnB dimer with the uracil buried at the interface and the UDP ribose and phosphate groups exposed to the solvent. The Ara4N moiety is found in the (4)C1 conformation and its positioning, stabilized by interactions with both the protein and cofactor, is compatible with catalysis. The structure suggests strategies for the development of specific inhibitors that may prove useful in the treatment of resistant bacteria such as Pseudomonas found in cystic fibrosis patients.
-
-	Structural Basis for Substrate Specificity in ArnB. A Key Enzyme in the Polymyxin Resistance Pathway of Gram-Negative Bacteria.,Lee M, Sousa MC Biochemistry. 2014 Feb 4;53(4):796-805. doi: 10.1021/bi4015677. Epub 2014 Jan 24. PMID:24460375<ref>PMID:24460375</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4oca" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:16, 25 January 2023

OCA — Wed, 25 Jan 2023 07:16:32 GMT

←Older revision		Revision as of 07:16, 25 January 2023
Line 1:		Line 1:

	==Cryatal structure of ArnB K188A complexted with PLP and UDP-Ara4N==		==Cryatal structure of ArnB K188A complexted with PLP and UDP-Ara4N==
-	<StructureSection load='4oca' size='340' side='right' caption='[[4oca]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='4oca' size='340' side='right'caption='[[4oca]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4oca]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OCA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4OCA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4oca]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OCA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2QR:(2R,3R,4S,5S)-3,4-dihydroxy-5-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]tetrahydro-2H-~~pyran~~-2-yl+[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl+dihydrogen+diphosphate'>2QR</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2QR:(2R,3R,4S,5S)-3,4-dihydroxy-5-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]tetrahydro-2H-pyr+an-2-yl+[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl+dihydrogen+diphosphate'>2QR</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">yfbE, arnB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oca OCA], [https://pdbe.org/4oca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oca RCSB], [https://www.ebi.ac.uk/pdbsum/4oca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oca ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-4-amino-4-deoxy-L-arabinose_aminotransferase UDP-4-amino-4-deoxy-L-arabinose aminotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.87 2.6.1.87] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4oca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oca OCA], [~~http~~://pdbe.org/4oca PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4oca RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4oca PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4oca ProSAT]</span></td></tr>	+
	</table>		</table>
-	== Function ==
-	[[http://www.uniprot.org/uniprot/F5ZUF5_SALTU F5ZUF5_SALTU]] Catalyzes the conversion of UDP-4-keto-arabinose (UDP-Ara4O) to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides (By similarity).[HAMAP-Rule:MF_01167][SAAS:SAAS022850_004_033570]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus typhimurium loeffler 1892~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~UDP-4-amino-4-deoxy-L-arabinose aminotransferase~~]]	+	[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
-	[[Category: Lee, M]]	+	[[Category: Lee M]]
-	[[Category: Sousa~~, M C]]~~	+	[[Category: Sousa MC]]
-	~~[[Category: Aminotransferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 14:24, 15 November 2017

OCA — Wed, 15 Nov 2017 14:24:48 GMT

←Older revision		Revision as of 14:24, 15 November 2017
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4oca]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OCA FirstGlance]. <br>		<table><tr><td colspan='2'>[[4oca]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OCA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2QR:(2R,3R,4S,5S)-3,4-~~DIHYDROXY~~-5-[({3-~~HYDROXY~~-2-~~METHYL~~-5-[(~~PHOSPHONOOXY~~)~~METHYL~~]~~PYRIDIN~~-4-YL}~~METHYL~~)~~AMINO~~]~~TETRAHYDRO~~-2H-~~PYRAN~~-2-YL+[(2R,3S,4R,5R)-5-(2,4-~~DIOXO~~-3,4-~~DIHYDROPYRIMIDIN~~-1(2H)-YL)-3,4-~~DIHYDROXYTETRAHYDROFURAN~~-2-YL]~~METHYL~~+~~DIHYDROGEN~~+~~DIPHOSPHATE~~'>2QR</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2QR:(2R,3R,4S,5S)-3,4-dihydroxy-5-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]tetrahydro-2H-pyran-2-yl+[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl+dihydrogen+diphosphate'>2QR</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">yfbE, arnB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">yfbE, arnB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-4-amino-4-deoxy-L-arabinose_aminotransferase UDP-4-amino-4-deoxy-L-arabinose aminotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.87 2.6.1.87] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-4-amino-4-deoxy-L-arabinose_aminotransferase UDP-4-amino-4-deoxy-L-arabinose aminotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.87 2.6.1.87] </span></td></tr>

OCA at 22:13, 4 August 2016

OCA — Thu, 04 Aug 2016 22:13:41 GMT

←Older revision		Revision as of 22:13, 4 August 2016
Line 1:		Line 1:
		+
	==Cryatal structure of ArnB K188A complexted with PLP and UDP-Ara4N==		==Cryatal structure of ArnB K188A complexted with PLP and UDP-Ara4N==
	<StructureSection load='4oca' size='340' side='right' caption='[[4oca]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4oca' size='340' side='right' caption='[[4oca]], [[Resolution\|resolution]] 2.30Å' scene=''>
Line 6:		Line 7:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">yfbE, arnB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">yfbE, arnB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-4-amino-4-deoxy-L-arabinose_aminotransferase UDP-4-amino-4-deoxy-L-arabinose aminotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.87 2.6.1.87] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-4-amino-4-deoxy-L-arabinose_aminotransferase UDP-4-amino-4-deoxy-L-arabinose aminotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.87 2.6.1.87] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oca OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4oca RCSB], [http://www.ebi.ac.uk/pdbsum/4oca PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oca OCA], [http://pdbe.org/4oca PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4oca RCSB], [http://www.ebi.ac.uk/pdbsum/4oca PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4oca ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4oca" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 20:08, 24 December 2014

OCA — Wed, 24 Dec 2014 20:08:36 GMT

←Older revision		Revision as of 20:08, 24 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4oca\| PDB=4oca \| SCENE= }}~~	+	==Cryatal structure of ArnB K188A complexted with PLP and UDP-Ara4N==
-	===Cryatal structure of ArnB K188A complexted with PLP and UDP-Ara4N===	+	<StructureSection load='4oca' size='340' side='right' caption='[[4oca]], [[Resolution\|resolution]] 2.30Å' scene=''>
-	{~~{ABSTRACT_PUBMED_24460375}~~}	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4oca]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OCA FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2QR:(2R,3R,4S,5S)-3,4-DIHYDROXY-5-[({3-HYDROXY-2-METHYL-5-[(PHOSPHONOOXY)METHYL]PYRIDIN-4-YL}METHYL)AMINO]TETRAHYDRO-2H-PYRAN-2-YL+[(2R,3S,4R,5R)-5-(2,4-DIOXO-3,4-DIHYDROPYRIMIDIN-1(2H)-YL)-3,4-DIHYDROXYTETRAHYDROFURAN-2-YL]METHYL+DIHYDROGEN+DIPHOSPHATE'>2QR</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">yfbE, arnB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-4-amino-4-deoxy-L-arabinose_aminotransferase UDP-4-amino-4-deoxy-L-arabinose aminotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.87 2.6.1.87] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oca OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4oca RCSB], [http://www.ebi.ac.uk/pdbsum/4oca PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/F5ZUF5_SALTU F5ZUF5_SALTU]] Catalyzes the conversion of UDP-4-keto-arabinose (UDP-Ara4O) to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides (By similarity).[HAMAP-Rule:MF_01167][SAAS:SAAS022850_004_033570]		[[http://www.uniprot.org/uniprot/F5ZUF5_SALTU F5ZUF5_SALTU]] Catalyzes the conversion of UDP-4-keto-arabinose (UDP-Ara4O) to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides (By similarity).[HAMAP-Rule:MF_01167][SAAS:SAAS022850_004_033570]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cationic Antimicrobial Peptides (CAMPs) represent a first line of defense against bacterial colonization. When fighting Gram-negative bacteria, CAMPs initially interact electrostatically with the negatively charged phosphate groups in lipid A and are thought to kill bacteria by disrupting their membrane integrity. However, many human pathogens, including Salmonella and Pseudomonas , have evolved lipid A modification mechanisms that result in resistance to CAMPs and related antibiotics such as Colistin. The addition of 4-amino-4-deoxy-l-Arabinose (Ara4N) to a phosphate group in lipid A is one such modification, frequently found in Pseudomonas isolated from cystic fibrosis patients. The pathway for biosynthesis of Ara4N-lipid A requires conversion of UDP-Glucuronic acid into UDP-Ara4N and subsequent transfer of the amino-sugar to lipid A. ArnB is a pyridoxal-phosphate (PLP) dependent transaminase that catalyzes a crucial step in the pathway: synthesis of UDP-Ara4N from UDP-4-keto-pentose. Here we present the 2.3 A resolution crystal structure of an active site mutant of ArnB (K188A) in complex with the reaction intermediate aldimine formed by UDP-Ara4N and PLP. The sugar-nucleotide binding site is in a cleft between the subunits of the ArnB dimer with the uracil buried at the interface and the UDP ribose and phosphate groups exposed to the solvent. The Ara4N moiety is found in the (4)C1 conformation and its positioning, stabilized by interactions with both the protein and cofactor, is compatible with catalysis. The structure suggests strategies for the development of specific inhibitors that may prove useful in the treatment of resistant bacteria such as Pseudomonas found in cystic fibrosis patients.

-	~~==About this Structure==~~	+	Structural Basis for Substrate Specificity in ArnB. A Key Enzyme in the Polymyxin Resistance Pathway of Gram-Negative Bacteria.,Lee M, Sousa MC Biochemistry. 2014 Feb 4;53(4):796-805. doi: 10.1021/bi4015677. Epub 2014 Jan 24. PMID:24460375<ref>PMID:24460375</ref>
-	~~[[4oca]] is a 1 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4OCA OCA]~~.	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024460375</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bacillus typhimurium loeffler 1892]]
	[[Category: UDP-4-amino-4-deoxy-L-arabinose aminotransferase]]		[[Category: UDP-4-amino-4-deoxy-L-arabinose aminotransferase]]
-	[[Category: Lee, M.]]	+	[[Category: Lee, M]]
-	[[Category: Sousa, M C.]]	+	[[Category: Sousa, M C]]
	[[Category: Aminotransferase]]		[[Category: Aminotransferase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 13:30, 12 March 2014

OCA — Wed, 12 Mar 2014 13:30:19 GMT

←Older revision		Revision as of 13:30, 12 March 2014
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4oca\| PDB=4oca \| SCENE= }}
		+	===Cryatal structure of ArnB K188A complexted with PLP and UDP-Ara4N===
		+	{{ABSTRACT_PUBMED_24460375}}

-	The ~~entry 4oca~~ is ~~ON HOLD~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/F5ZUF5_SALTU F5ZUF5_SALTU]] Catalyzes the conversion of UDP-4-keto-arabinose (UDP-Ara4O) to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides (By similarity).[HAMAP-Rule:MF_01167][SAAS:SAAS022850_004_033570]

-	~~Authors~~: ~~Sousa, M~~.C.~~, Lee, M~~.	+	==About this Structure==
		+	[[4oca]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OCA OCA].

-	~~Description~~: ~~Cryatal structure of ArnB K188A complexted with PLP and~~ UDP-~~Ara4N~~	+	==Reference==
		+	<ref group="xtra">PMID:024460375</ref><references group="xtra"/><references/>
		+	[[Category: UDP-4-amino-4-deoxy-L-arabinose aminotransferase]]
		+	[[Category: Lee, M.]]
		+	[[Category: Sousa, M C.]]
		+	[[Category: Aminotransferase]]
		+	[[Category: Transferase]]

OCA: Protected "4oca" [edit=sysop:move=sysop]

OCA — Wed, 15 Jan 2014 07:46:15 GMT

Protected "4oca" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:46, 15 January 2014

OCA: New page: '''Unreleased structure''' The entry 4oca is ON HOLD Authors: Sousa, M.C., Lee, M. Description: Cryatal structure of ArnB K188A complexted with PLP and UDP-Ara4N

OCA — Wed, 15 Jan 2014 07:46:14 GMT

New page: '''Unreleased structure''' The entry 4oca is ON HOLD Authors: Sousa, M.C., Lee, M. Description: Cryatal structure of ArnB K188A complexted with PLP and UDP-Ara4N

New page

'''Unreleased structure'''

The entry 4oca is ON HOLD

Authors: Sousa, M.C., Lee, M.

Description: Cryatal structure of ArnB K188A complexted with PLP and UDP-Ara4N