4opu - Revision history

OCA at 07:39, 25 January 2023

OCA — Wed, 25 Jan 2023 07:39:11 GMT

←Older revision		Revision as of 07:39, 25 January 2023
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	<StructureSection load='4opu' size='340' side='right'caption='[[4opu]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='4opu' size='340' side='right'caption='[[4opu]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4opu]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Sulac Sulac~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OPU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4OPU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4opu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius_DSM_639 Sulfolobus acidocaldarius DSM 639]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OPU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OPU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=GRG:GERANYLGERANYL+DIPHOSPHATE'>GRG</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=GRG:GERANYLGERANYL+DIPHOSPHATE'>GRG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4opc\|4opc]], [[4opd\|4opd]], [[4opg\|4opg]], [[4opi\|4opi]], [[4opl\|4opl]], [[4opt\|4opt]]</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4opu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4opu OCA], [https://pdbe.org/4opu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4opu RCSB], [https://www.ebi.ac.uk/pdbsum/4opu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4opu ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Saci_0986 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=330779 SULAC])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Geranylgeranyl_diphosphate_reductase Geranylgeranyl diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.83 1.3.1.83] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4opu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4opu OCA], [~~http~~://pdbe.org/4opu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4opu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4opu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4opu ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GGR_SULAC GGR_SULAC] Is involved in the reduction of 2,3-digeranylgeranylglycerophospholipids (unsaturated archaeols) into 2,3-diphytanylglycerophospholipids (saturated archaeols) in the biosynthesis of archaeal membrane lipids. Catalyzes the formation of archaetidic acid (2,3-di-O-phytanyl-sn-glyceryl phosphate) from 2,3-di-O-geranylgeranylglyceryl phosphate (DGGGP) via the hydrogenation of each double bond of the isoprenoid chains. Is not active with NADPH or NADH as an electron donor; the physiological reducing agent is unknown. Is also active on the more upstream precursors of membrane lipid biosynthesis, catalyzing the complete reduction of 3-O-geranylgeranylglyceryl phosphate (GGGP) to 3-O-phytanylglyceryl phosphate, and the partial reduction of geranylgeranyl diphosphate (GGPP) to phytyl diphosphate, thus reducing three of four GGPP double bonds and preserving the allylic double bond (at position 2). This reaction product is a reactive prenyl donor, which can be used as a substrate by archaeal prenyltransferases such as GGGP synthases.<ref>PMID:18375567</ref> <ref>PMID:21515284</ref> <ref>PMID:24954619</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Geranylgeranyl diphosphate reductase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Sulac~~]]	+	[[Category: Sulfolobus acidocaldarius DSM 639]]
-	[[Category: Adams~~, P D~~]]	+	[[Category: Adams PD]]
-	[[Category: Keasling~~, J D~~]]	+	[[Category: Keasling JD]]
-	[[Category: Kung, Y]]	+	[[Category: Kung Y]]
-	[[Category: Liu, C]]	+	[[Category: Liu C]]
-	[[Category: McAndrew~~, R P~~]]	+	[[Category: McAndrew RP]]
-	[[Category: Pereira~~, J H~~]]	+	[[Category: Pereira JH]]
-	[[Category: Xie, X]]	+	[[Category: Xie X]]
-	~~[[Category: Archaeal protein]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Rossmann fold~~]]	+

OCA at 07:25, 17 April 2019

OCA — Wed, 17 Apr 2019 07:25:26 GMT

←Older revision		Revision as of 07:25, 17 April 2019
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	==Constructing tailored isoprenoid products by structure-guided modification of geranylgeranyl reductase==		==Constructing tailored isoprenoid products by structure-guided modification of geranylgeranyl reductase==
-	<StructureSection load='4opu' size='340' side='right' caption='[[4opu]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='4opu' size='340' side='right'caption='[[4opu]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4opu]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OPU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OPU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4opu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulac Sulac]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OPU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OPU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=GRG:GERANYLGERANYL+DIPHOSPHATE'>GRG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=GRG:GERANYLGERANYL+DIPHOSPHATE'>GRG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4opc\|4opc]], [[4opd\|4opd]], [[4opg\|4opg]], [[4opi\|4opi]], [[4opl\|4opl]], [[4opt\|4opt]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4opc\|4opc]], [[4opd\|4opd]], [[4opg\|4opg]], [[4opi\|4opi]], [[4opl\|4opl]], [[4opt\|4opt]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Saci_0986 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=330779 SULAC])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Geranylgeranyl_diphosphate_reductase Geranylgeranyl diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.83 1.3.1.83] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Geranylgeranyl_diphosphate_reductase Geranylgeranyl diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.83 1.3.1.83] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4opu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4opu OCA], [http://pdbe.org/4opu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4opu RCSB], [http://www.ebi.ac.uk/pdbsum/4opu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4opu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4opu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4opu OCA], [http://pdbe.org/4opu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4opu RCSB], [http://www.ebi.ac.uk/pdbsum/4opu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4opu ProSAT]</span></td></tr>
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	</StructureSection>		</StructureSection>
	[[Category: Geranylgeranyl diphosphate reductase]]		[[Category: Geranylgeranyl diphosphate reductase]]
		+	[[Category: Large Structures]]
		+	[[Category: Sulac]]
	[[Category: Adams, P D]]		[[Category: Adams, P D]]
	[[Category: Keasling, J D]]		[[Category: Keasling, J D]]

OCA at 08:13, 11 August 2016

OCA — Thu, 11 Aug 2016 08:13:21 GMT

←Older revision		Revision as of 08:13, 11 August 2016
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		+
	==Constructing tailored isoprenoid products by structure-guided modification of geranylgeranyl reductase==		==Constructing tailored isoprenoid products by structure-guided modification of geranylgeranyl reductase==
	<StructureSection load='4opu' size='340' side='right' caption='[[4opu]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='4opu' size='340' side='right' caption='[[4opu]], [[Resolution\|resolution]] 2.70Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4opc\|4opc]], [[4opd\|4opd]], [[4opg\|4opg]], [[4opi\|4opi]], [[4opl\|4opl]], [[4opt\|4opt]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4opc\|4opc]], [[4opd\|4opd]], [[4opg\|4opg]], [[4opi\|4opi]], [[4opl\|4opl]], [[4opt\|4opt]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Geranylgeranyl_diphosphate_reductase Geranylgeranyl diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.83 1.3.1.83] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Geranylgeranyl_diphosphate_reductase Geranylgeranyl diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.83 1.3.1.83] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4opu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4opu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4opu RCSB], [http://www.ebi.ac.uk/pdbsum/4opu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4opu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4opu OCA], [http://pdbe.org/4opu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4opu RCSB], [http://www.ebi.ac.uk/pdbsum/4opu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4opu ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4opu" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:29, 5 January 2015

OCA — Mon, 05 Jan 2015 12:29:58 GMT

←Older revision		Revision as of 12:29, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4opu]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OPU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OPU FirstGlance]. <br>		<table><tr><td colspan='2'>[[4opu]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OPU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OPU FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=GRG:GERANYLGERANYL+DIPHOSPHATE'>GRG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=GRG:GERANYLGERANYL+DIPHOSPHATE'>GRG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4opc\|4opc]], [[4opd\|4opd]], [[4opg\|4opg]], [[4opi\|4opi]], [[4opl\|4opl]], [[4opt\|4opt]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4opc\|4opc]], [[4opd\|4opd]], [[4opg\|4opg]], [[4opi\|4opi]], [[4opl\|4opl]], [[4opt\|4opt]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Geranylgeranyl_diphosphate_reductase Geranylgeranyl diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.83 1.3.1.83] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Geranylgeranyl_diphosphate_reductase Geranylgeranyl diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.83 1.3.1.83] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4opu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4opu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4opu RCSB], [http://www.ebi.ac.uk/pdbsum/4opu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4opu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4opu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4opu RCSB], [http://www.ebi.ac.uk/pdbsum/4opu PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Geranylgeranyl diphosphate reductase]]		[[Category: Geranylgeranyl diphosphate reductase]]
-	[[Category: Adams, P D.]]	+	[[Category: Adams, P D]]
-	[[Category: Keasling, J D.]]	+	[[Category: Keasling, J D]]
-	[[Category: Kung, Y.]]	+	[[Category: Kung, Y]]
-	[[Category: Liu, C.]]	+	[[Category: Liu, C]]
-	[[Category: McAndrew, R P.]]	+	[[Category: McAndrew, R P]]
-	[[Category: Pereira, J H.]]	+	[[Category: Pereira, J H]]
-	[[Category: Xie, X.]]	+	[[Category: Xie, X]]
	[[Category: Archaeal protein]]		[[Category: Archaeal protein]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Rossmann fold]]		[[Category: Rossmann fold]]

OCA at 07:20, 9 July 2014

OCA — Wed, 09 Jul 2014 07:20:20 GMT

←Older revision		Revision as of 07:20, 9 July 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Constructing tailored isoprenoid products by structure-guided modification of geranylgeranyl reductase==
		+	<StructureSection load='4opu' size='340' side='right' caption='[[4opu]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4opu]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OPU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OPU FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FDA:DIHYDROFLAVINE-ADENINE+DINUCLEOTIDE'>FDA</scene>, <scene name='pdbligand=GRG:GERANYLGERANYL+DIPHOSPHATE'>GRG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4opc\|4opc]], [[4opd\|4opd]], [[4opg\|4opg]], [[4opi\|4opi]], [[4opl\|4opl]], [[4opt\|4opt]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Geranylgeranyl_diphosphate_reductase Geranylgeranyl diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.83 1.3.1.83] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4opu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4opu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4opu RCSB], [http://www.ebi.ac.uk/pdbsum/4opu PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The archaeal enzyme geranylgeranyl reductase (GGR) catalyzes hydrogenation of carbon-carbon double bonds to produce the saturated alkyl chains of the organism's unusual isoprenoid-derived cell membrane. Enzymatic reduction of isoprenoid double bonds is of considerable interest both to natural products researchers and to synthetic biologists interested in the microbial production of isoprenoid drug or biofuel molecules. Here we present crystal structures of GGR from Sulfolobus acidocaldarius, including the structure of GGR bound to geranylgeranyl pyrophosphate (GGPP). The structures are presented alongside activity data that depict the sequential reduction of GGPP to H6GGPP via the intermediates H2GGPP and H4GGPP. We then modified the enzyme to generate sequence variants that display increased rates of H6GGPP production or are able to halt the extent of reduction at H2GGPP and H4GGPP. Crystal structures of these variants not only reveal the structural bases for their altered activities; they also shed light onto the catalytic mechanism employed.

-	~~The entry 4opu is ON HOLD until Paper Publication~~	+	Constructing Tailored Isoprenoid Products by Structure-Guided Modification of Geranylgeranyl Reductase.,Kung Y, McAndrew RP, Xie X, Liu CC, Pereira JH, Adams PD, Keasling JD Structure. 2014 Jun 17. pii: S0969-2126(14)00148-8. doi:, 10.1016/j.str.2014.05.007. PMID:24954619<ref>PMID:24954619</ref>

-	~~Authors: McAndrew~~, R.P., Kung, Y.~~, Xie, X.,~~ Liu, C., Pereira, J.H., ~~Keasling, J.D., Adams, P.D~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Constructing tailored isoprenoid products by structure-guided modification of geranylgeranyl reductase~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Geranylgeranyl diphosphate reductase]]
		+	[[Category: Adams, P D.]]
		+	[[Category: Keasling, J D.]]
		+	[[Category: Kung, Y.]]
		+	[[Category: Liu, C.]]
		+	[[Category: McAndrew, R P.]]
		+	[[Category: Pereira, J H.]]
		+	[[Category: Xie, X.]]
		+	[[Category: Archaeal protein]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Rossmann fold]]

OCA at 07:19, 23 April 2014

OCA — Wed, 23 Apr 2014 07:19:51 GMT

←Older revision		Revision as of 07:19, 23 April 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4opu is ON HOLD	+	The entry 4opu is ON HOLD until Paper Publication

	Authors: McAndrew, R.P., Kung, Y., Xie, X., Liu, C., Pereira, J.H., Keasling, J.D., Adams, P.D.		Authors: McAndrew, R.P., Kung, Y., Xie, X., Liu, C., Pereira, J.H., Keasling, J.D., Adams, P.D.

	Description: Constructing tailored isoprenoid products by structure-guided modification of geranylgeranyl reductase		Description: Constructing tailored isoprenoid products by structure-guided modification of geranylgeranyl reductase

OCA: Protected "4opu" [edit=sysop:move=sysop]

OCA — Thu, 13 Feb 2014 04:52:37 GMT

Protected "4opu" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:52, 13 February 2014

OCA: New page: '''Unreleased structure''' The entry 4opu is ON HOLD Authors: McAndrew, R.P., Kung, Y., Xie, X., Liu, C., Pereira, J.H., Keasling, J.D., Adams, P.D. Description: Constructing tailored ...

OCA — Thu, 13 Feb 2014 04:52:36 GMT

New page: '''Unreleased structure''' The entry 4opu is ON HOLD Authors: McAndrew, R.P., Kung, Y., Xie, X., Liu, C., Pereira, J.H., Keasling, J.D., Adams, P.D. Description: Constructing tailored ...

New page

'''Unreleased structure'''

The entry 4opu is ON HOLD

Authors: McAndrew, R.P., Kung, Y., Xie, X., Liu, C., Pereira, J.H., Keasling, J.D., Adams, P.D.

Description: Constructing tailored isoprenoid products by structure-guided modification of geranylgeranyl reductase