4ou5 - Revision history

OCA at 14:59, 8 November 2023

OCA — Wed, 08 Nov 2023 14:59:44 GMT

←Older revision		Revision as of 14:59, 8 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ou5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._ECU1011 Pseudomonas sp. ECU1011]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OU5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OU5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ou5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._ECU1011 Pseudomonas sp. ECU1011]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OU5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OU5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.99Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ou5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ou5 OCA], [https://pdbe.org/4ou5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ou5 RCSB], [https://www.ebi.ac.uk/pdbsum/4ou5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ou5 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ou5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ou5 OCA], [https://pdbe.org/4ou5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ou5 RCSB], [https://www.ebi.ac.uk/pdbsum/4ou5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ou5 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:46, 25 January 2023

OCA — Wed, 25 Jan 2023 07:46:08 GMT

←Older revision		Revision as of 07:46, 25 January 2023
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	<StructureSection load='4ou5' size='340' side='right'caption='[[4ou5]], [[Resolution\|resolution]] 1.99Å' scene=''>		<StructureSection load='4ou5' size='340' side='right'caption='[[4ou5]], [[Resolution\|resolution]] 1.99Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ou5]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Pseudomonas_sp.~~_ecu1011~~ Pseudomonas sp. ~~ecu1011~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OU5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4OU5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ou5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._ECU1011 Pseudomonas sp. ECU1011]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OU5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OU5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ob6\|4ob6]], [[4ob7\|4ob7]], [[4ob8\|4ob8]], [[4ou4\|4ou4]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ou5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ou5 OCA], [https://pdbe.org/4ou5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ou5 RCSB], [https://www.ebi.ac.uk/pdbsum/4ou5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ou5 ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ou5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ou5 OCA], [~~http~~://pdbe.org/4ou5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ou5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ou5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ou5 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/L7PYQ2_9PSED L7PYQ2_9PSED]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Carboxylesterase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Pseudomonas sp. ~~ecu1011~~]]	+	[[Category: Pseudomonas sp. ECU1011]]
-	[[Category: Dou, S]]	+	[[Category: Dou S]]
-	[[Category: Kong~~, X D~~]]	+	[[Category: Kong XD]]
-	[[Category: Ma~~, B D~~]]	+	[[Category: Ma BD]]
-	[[Category: Xu~~, J H~~]]	+	[[Category: Xu JH]]
-	[[Category: Zhou~~, J H]]~~	+	[[Category: Zhou JH]]
-	~~[[Category: A/b hydrolase fold]]~~	+
-	~~[[Category: Esterase]]~~	+
-	~~[[Category: Hsl-like family]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 08:09, 19 February 2020

OCA — Wed, 19 Feb 2020 08:09:11 GMT

←Older revision		Revision as of 08:09, 19 February 2020
Line 1:		Line 1:

	==Crystal structure of esterase rPPE mutant S159A/W187H==		==Crystal structure of esterase rPPE mutant S159A/W187H==
-	<StructureSection load='4ou5' size='340' side='right' caption='[[4ou5]], [[Resolution\|resolution]] 1.99Å' scene=''>	+	<StructureSection load='4ou5' size='340' side='right'caption='[[4ou5]], [[Resolution\|resolution]] 1.99Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ou5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp._ecu1011 Pseudomonas sp. ecu1011]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OU5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OU5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ou5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp._ecu1011 Pseudomonas sp. ecu1011]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OU5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OU5 FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Carboxylesterase]]		[[Category: Carboxylesterase]]
		+	[[Category: Large Structures]]
	[[Category: Pseudomonas sp. ecu1011]]		[[Category: Pseudomonas sp. ecu1011]]
	[[Category: Dou, S]]		[[Category: Dou, S]]

OCA at 08:30, 22 November 2017

OCA — Wed, 22 Nov 2017 08:30:22 GMT

←Older revision		Revision as of 08:30, 22 November 2017
Line 3:		Line 3:
	<StructureSection load='4ou5' size='340' side='right' caption='[[4ou5]], [[Resolution\|resolution]] 1.99Å' scene=''>		<StructureSection load='4ou5' size='340' side='right' caption='[[4ou5]], [[Resolution\|resolution]] 1.99Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ou5]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OU5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OU5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ou5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp._ecu1011 Pseudomonas sp. ecu1011]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OU5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OU5 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ob6\|4ob6]], [[4ob7\|4ob7]], [[4ob8\|4ob8]], [[4ou4\|4ou4]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ob6\|4ob6]], [[4ob7\|4ob7]], [[4ob8\|4ob8]], [[4ou4\|4ou4]]</td></tr>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Carboxylesterase]]		[[Category: Carboxylesterase]]
		+	[[Category: Pseudomonas sp. ecu1011]]
	[[Category: Dou, S]]		[[Category: Dou, S]]
	[[Category: Kong, X D]]		[[Category: Kong, X D]]

OCA at 19:08, 11 August 2016

OCA — Thu, 11 Aug 2016 19:08:08 GMT

←Older revision		Revision as of 19:08, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of esterase rPPE mutant S159A/W187H==		==Crystal structure of esterase rPPE mutant S159A/W187H==
	<StructureSection load='4ou5' size='340' side='right' caption='[[4ou5]], [[Resolution\|resolution]] 1.99Å' scene=''>		<StructureSection load='4ou5' size='340' side='right' caption='[[4ou5]], [[Resolution\|resolution]] 1.99Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ob6\|4ob6]], [[4ob7\|4ob7]], [[4ob8\|4ob8]], [[4ou4\|4ou4]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ob6\|4ob6]], [[4ob7\|4ob7]], [[4ob8\|4ob8]], [[4ou4\|4ou4]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ou5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ou5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ou5 RCSB], [http://www.ebi.ac.uk/pdbsum/4ou5 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ou5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ou5 OCA], [http://pdbe.org/4ou5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ou5 RCSB], [http://www.ebi.ac.uk/pdbsum/4ou5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ou5 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4ou5" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:52, 5 January 2015

OCA — Mon, 05 Jan 2015 12:52:15 GMT

←Older revision		Revision as of 12:52, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ou5]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OU5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OU5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ou5]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OU5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OU5 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ob6\|4ob6]], [[4ob7\|4ob7]], [[4ob8\|4ob8]], [[4ou4\|4ou4]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ob6\|4ob6]], [[4ob7\|4ob7]], [[4ob8\|4ob8]], [[4ou4\|4ou4]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ou5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ou5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ou5 RCSB], [http://www.ebi.ac.uk/pdbsum/4ou5 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ou5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ou5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ou5 RCSB], [http://www.ebi.ac.uk/pdbsum/4ou5 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Carboxylesterase]]		[[Category: Carboxylesterase]]
-	[[Category: Dou, S.]]	+	[[Category: Dou, S]]
-	[[Category: Kong, X D.]]	+	[[Category: Kong, X D]]
-	[[Category: Ma, B D.]]	+	[[Category: Ma, B D]]
-	[[Category: Xu, J H.]]	+	[[Category: Xu, J H]]
-	[[Category: Zhou, J H.]]	+	[[Category: Zhou, J H]]
	[[Category: A/b hydrolase fold]]		[[Category: A/b hydrolase fold]]
	[[Category: Esterase]]		[[Category: Esterase]]
	[[Category: Hsl-like family]]		[[Category: Hsl-like family]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 07:53, 23 July 2014

OCA — Wed, 23 Jul 2014 07:53:52 GMT

←Older revision		Revision as of 07:53, 23 July 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of esterase rPPE mutant S159A/W187H==
		+	<StructureSection load='4ou5' size='340' side='right' caption='[[4ou5]], [[Resolution\|resolution]] 1.99Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4ou5]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OU5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OU5 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ob6\|4ob6]], [[4ob7\|4ob7]], [[4ob8\|4ob8]], [[4ou4\|4ou4]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ou5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ou5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ou5 RCSB], [http://www.ebi.ac.uk/pdbsum/4ou5 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A recombinant carboxylesterase (rPPE) from Pseudomonas putida ECU1011 was previously cloned and engineered to give a potential application for resolving chiral alpha-hydroxy acids including mandelic acids and derivatives. Two variants rPPEW187H and rPPED287A showed a approximately 100-fold increase in activity towards rac-2-acetoxy-2-(2'-chlorophenyl) acetate (rac-AcO-CPA), but rPPED287A had a significant decrease in enantioselectivity (E=8.7) compared to rPPEW187H and the wild-type rPPE (rPPEWT) (E>200). Here we report the crystal structures of rPPEWT and rPPEW187H, both by themselves and in complex with the substrate, to elucidate the structural basis of this phenomenon. An inactive mutation of nucleophile residue S159A was introduced to obtain the structure of rPPES159A/W187H complexed with (S)-AcO-CPA. The structural analysis reveals that the side chain of residue Asp287 in rPPEWT would have a potential steric conflict with (S)-AcO-CPA when the substrate binds at the active site of the enzyme. However, the mutation W187H could facilitate the relocation of Asp287, while D287A directly eliminates the hindrance of Asp287, both of which offer sufficient space for the binding and hydrolysis of substrate. Moreover, Asp287 generates one site of the "three-point attachment model" as a hydrogen-bond donor that determines the excellent enantioselectivity of rPPE in chiral recognition, and D287A would obviously destroy the hydrogen bond and result in the low enantioselectivity of rPPED287A.

-	~~The entry 4ou5 is ON HOLD~~	+	Crystal structures of Pseudomonas putida esterase reveal the functional role of residues 187 and 287 in substrate binding and chiral recognition.,Dou S, Kong XD, Ma BD, Chen Q, Zhang J, Zhou J, Xu JH Biochem Biophys Res Commun. 2014 Apr 18;446(4):1145-50. doi:, 10.1016/j.bbrc.2014.03.072. Epub 2014 Mar 27. PMID:24680822<ref>PMID:24680822</ref>

-	~~Authors~~: Dou, S., Kong, X.D., Ma, B.D., Xu, J.H., Zhou, J.H.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of esterase rPPE mutant S159A~~/~~W187H~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Carboxylesterase]]
		+	[[Category: Dou, S.]]
		+	[[Category: Kong, X D.]]
		+	[[Category: Ma, B D.]]
		+	[[Category: Xu, J H.]]
		+	[[Category: Zhou, J H.]]
		+	[[Category: A/b hydrolase fold]]
		+	[[Category: Esterase]]
		+	[[Category: Hsl-like family]]
		+	[[Category: Hydrolase]]

OCA at 12:21, 12 March 2014

OCA — Wed, 12 Mar 2014 12:21:38 GMT

←Older revision		Revision as of 12:21, 12 March 2014
Line 3:		Line 3:
	The entry 4ou5 is ON HOLD		The entry 4ou5 is ON HOLD

-	Authors: Dou S., Kong X.D., Ma B.D., Xu J.H., Zhou J.H.	+	Authors: Dou, S., Kong, X.D., Ma, B.D., Xu, J.H., Zhou, J.H.

	Description: Crystal structure of esterase rPPE mutant S159A/W187H		Description: Crystal structure of esterase rPPE mutant S159A/W187H

OCA: Protected "4ou5" [edit=sysop:move=sysop]

OCA — Mon, 03 Mar 2014 16:52:10 GMT

Protected "4ou5" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:52, 3 March 2014

OCA: New page: '''Unreleased structure''' The entry 4ou5 is ON HOLD Authors: Dou S., Kong X.D., Ma B.D., Xu J.H., Zhou J.H. Description: Crystal structure of esterase rPPE mutant S159A/W187H

OCA — Mon, 03 Mar 2014 16:52:08 GMT

New page: '''Unreleased structure''' The entry 4ou5 is ON HOLD Authors: Dou S., Kong X.D., Ma B.D., Xu J.H., Zhou J.H. Description: Crystal structure of esterase rPPE mutant S159A/W187H

New page

'''Unreleased structure'''

The entry 4ou5 is ON HOLD

Authors: Dou S., Kong X.D., Ma B.D., Xu J.H., Zhou J.H.

Description: Crystal structure of esterase rPPE mutant S159A/W187H