4oxq - Revision history

OCA at 07:07, 27 September 2023

OCA — Wed, 27 Sep 2023 07:07:07 GMT

←Older revision		Revision as of 07:07, 27 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4oxq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_pseudintermedius Staphylococcus pseudintermedius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OXQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OXQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4oxq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_pseudintermedius Staphylococcus pseudintermedius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OXQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OXQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.62Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oxq OCA], [https://pdbe.org/4oxq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oxq RCSB], [https://www.ebi.ac.uk/pdbsum/4oxq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oxq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oxq OCA], [https://pdbe.org/4oxq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oxq RCSB], [https://www.ebi.ac.uk/pdbsum/4oxq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oxq ProSAT]</span></td></tr>
	</table>		</table>

OCA at 12:31, 1 February 2023

OCA — Wed, 01 Feb 2023 12:31:26 GMT

←Older revision		Revision as of 12:31, 1 February 2023
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	==Structure of Staphylococcus pseudintermedius metal-binding protein SitA in complex with Zinc==		==Structure of Staphylococcus pseudintermedius metal-binding protein SitA in complex with Zinc==
-	<StructureSection load='4oxq' size='340' side='right' caption='[[4oxq]], [[Resolution\|resolution]] 2.62Å' scene=''>	+	<StructureSection load='4oxq' size='340' side='right'caption='[[4oxq]], [[Resolution\|resolution]] 2.62Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4oxq]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ccug_49543 Ccug 49543~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OXQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4OXQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4oxq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_pseudintermedius Staphylococcus pseudintermedius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OXQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OXQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4oxr\|4oxr]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oxq OCA], [https://pdbe.org/4oxq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oxq RCSB], [https://www.ebi.ac.uk/pdbsum/4oxq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oxq ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SPSE_2131 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=283734 CCUG 49543])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4oxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oxq OCA], [~~http~~://pdbe.org/4oxq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4oxq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4oxq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4oxq ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ccug 49543~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Abate, F~~]]	+	[[Category: Staphylococcus pseudintermedius]]
-	[[Category: ~~Bottomley, M~~]]	+	[[Category: Abate F]]
-	[[Category: ~~Malito, E~~]]	+	[[Category: Bottomley M]]
-	[[Category: ~~Abc transporter]]~~	+	[[Category: Malito E]]
-	~~[[Category: Manganese binding protein]]~~	+
-	~~[[Category: Metal binding protein]]~~	+
-	~~[[Category: Sbp~~]]	+

OCA at 08:31, 22 November 2017

OCA — Wed, 22 Nov 2017 08:31:33 GMT

←Older revision		Revision as of 08:31, 22 November 2017
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	<StructureSection load='4oxq' size='340' side='right' caption='[[4oxq]], [[Resolution\|resolution]] 2.62Å' scene=''>		<StructureSection load='4oxq' size='340' side='right' caption='[[4oxq]], [[Resolution\|resolution]] 2.62Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4oxq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OXQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OXQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4oxq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ccug_49543 Ccug 49543]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OXQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OXQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4oxr\|4oxr]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4oxr\|4oxr]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SPSE_2131 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=283734 CCUG 49543])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oxq OCA], [http://pdbe.org/4oxq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4oxq RCSB], [http://www.ebi.ac.uk/pdbsum/4oxq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4oxq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oxq OCA], [http://pdbe.org/4oxq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4oxq RCSB], [http://www.ebi.ac.uk/pdbsum/4oxq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4oxq ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ccug 49543]]
	[[Category: Abate, F]]		[[Category: Abate, F]]
	[[Category: Bottomley, M]]		[[Category: Bottomley, M]]
Line 26:		Line 28:
	[[Category: Abc transporter]]		[[Category: Abc transporter]]
	[[Category: Manganese binding protein]]		[[Category: Manganese binding protein]]
		+	[[Category: Metal binding protein]]
	[[Category: Sbp]]		[[Category: Sbp]]

OCA at 16:51, 2 January 2017

OCA — Mon, 02 Jan 2017 16:51:17 GMT

←Older revision		Revision as of 16:51, 2 January 2017
Line 1:		Line 1:
		+
	==Structure of Staphylococcus pseudintermedius metal-binding protein SitA in complex with Zinc==		==Structure of Staphylococcus pseudintermedius metal-binding protein SitA in complex with Zinc==
	<StructureSection load='4oxq' size='340' side='right' caption='[[4oxq]], [[Resolution\|resolution]] 2.62Å' scene=''>		<StructureSection load='4oxq' size='340' side='right' caption='[[4oxq]], [[Resolution\|resolution]] 2.62Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4oxr\|4oxr]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4oxr\|4oxr]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oxq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4oxq RCSB], [http://www.ebi.ac.uk/pdbsum/4oxq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oxq OCA], [http://pdbe.org/4oxq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4oxq RCSB], [http://www.ebi.ac.uk/pdbsum/4oxq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4oxq ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4oxq" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:25, 24 December 2014

OCA — Wed, 24 Dec 2014 11:25:57 GMT

←Older revision		Revision as of 11:25, 24 December 2014
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Abate, F.]]	+	[[Category: Abate, F]]
-	[[Category: Bottomley, M.]]	+	[[Category: Bottomley, M]]
-	[[Category: Malito, E.]]	+	[[Category: Malito, E]]
	[[Category: Abc transporter]]		[[Category: Abc transporter]]
	[[Category: Manganese binding protein]]		[[Category: Manganese binding protein]]
	[[Category: Sbp]]		[[Category: Sbp]]

OCA at 11:13, 29 October 2014

OCA — Wed, 29 Oct 2014 11:13:05 GMT

←Older revision		Revision as of 11:13, 29 October 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Structure of Staphylococcus pseudintermedius metal-binding protein SitA in complex with Zinc==
		+	<StructureSection load='4oxq' size='340' side='right' caption='[[4oxq]], [[Resolution\|resolution]] 2.62Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4oxq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OXQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OXQ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4oxr\|4oxr]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oxq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4oxq RCSB], [http://www.ebi.ac.uk/pdbsum/4oxq PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Gram-positive bacterium Staphylococcus pseudintermedius is a leading cause of canine bacterial pyoderma, resulting in worldwide morbidity in dogs. S. pseudintermedius also causes life-threatening human infections. Further, methicillin-resistant S. pseudintermedius is emerging, resembling the human health threat of methicillin-resistant Staphylococcus aureus. Therefore, it is increasingly important to characterize targets for intervention strategies to counteract S. pseudintermedius infections. Here we used biophysical methods, mutagenesis, and X-ray crystallography, to define the ligand-binding properties and structure of SitA, a S. pseudintermedius surface lipoprotein. SitA was strongly and specifically stabilized by Mn2+ and Zn2+ ions. Crystal structures of SitA complexed with Mn2+ and Zn2+ revealed a canonical class III solute-binding protein with the metal cation bound in a cavity between N- and C-terminal lobes. Unexpectedly, one crystal contained both apo- and holo-forms of SitA, revealing a large side-chain reorientation of His64, and associated structural differences accompanying ligand binding. Such conformational changes may regulate fruitful engagement of the cognate ATP-binding cassette (ABC) transporter system (SitBC) required for metal uptake. These results provide the first detailed characterization and mechanistic insights for a potential therapeutic target of the major canine pathogen S. pseudintermedius, and also shed light on homologous structures in related staphylococcal pathogens afflicting humans.

-	~~The entry 4oxq is ON HOLD until Paper Publication~~	+	Apo, Zn2+-bound and Mn2+-bound structures reveal ligand binding properties of SitA from the pathogen Staphylococcus pseudintermedius.,Abate F, Malito E, Cozzi R, Lo Surdo P, Maione D, Bottomley MJ Biosci Rep. 2014 Oct 14. PMID:25311310<ref>PMID:25311310</ref>

-	~~Authors~~: Abate, F., Malito, E.~~, Bottomley, M.~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Structure of Staphylococcus pseudintermedius metal-~~binding protein ~~SitA in complex with Zinc~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Abate, F.]]
		+	[[Category: Bottomley, M.]]
		+	[[Category: Malito, E.]]
		+	[[Category: Abc transporter]]
		+	[[Category: Manganese binding protein]]
		+	[[Category: Sbp]]

OCA at 12:23, 12 March 2014

OCA — Wed, 12 Mar 2014 12:23:43 GMT

←Older revision		Revision as of 12:23, 12 March 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4oxq is ON HOLD	+	The entry 4oxq is ON HOLD until Paper Publication

	Authors: Abate, F., Malito, E., Bottomley, M.		Authors: Abate, F., Malito, E., Bottomley, M.

	Description: Structure of Staphylococcus pseudintermedius metal-binding protein SitA in complex with Zinc		Description: Structure of Staphylococcus pseudintermedius metal-binding protein SitA in complex with Zinc

OCA: Protected "4oxq" [edit=sysop:move=sysop]

OCA — Thu, 13 Feb 2014 04:53:34 GMT

Protected "4oxq" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:53, 13 February 2014

OCA: New page: '''Unreleased structure''' The entry 4oxq is ON HOLD Authors: Abate, F., Malito, E., Bottomley, M. Description: Structure of Staphylococcus pseudintermedius metal-binding protein SitA ...

OCA — Thu, 13 Feb 2014 04:53:33 GMT

New page: '''Unreleased structure''' The entry 4oxq is ON HOLD Authors: Abate, F., Malito, E., Bottomley, M. Description: Structure of Staphylococcus pseudintermedius metal-binding protein SitA ...

New page

'''Unreleased structure'''

The entry 4oxq is ON HOLD

Authors: Abate, F., Malito, E., Bottomley, M.

Description: Structure of Staphylococcus pseudintermedius metal-binding protein SitA in complex with Zinc