4oyg - Revision history

OCA: Redirecting to 5cfy

OCA — Wed, 11 May 2016 03:20:54 GMT

Redirecting to 5cfy

←Older revision		Revision as of 03:20, 11 May 2016
Line 1:		Line 1:
-	~~==Crystal structure of GltPh R397A in complex with Na+ and L-asp==~~	+	#REDIRECT [[5cfy]] This PDB entry is obsolete and replaced by 5cfy
-	~~<StructureSection load='4oyg' size='340' side='right' caption='~~[[~~4oyg]], [[Resolution\|resolution]] 3.50Å' scene=''>~~	+
-	~~== Structural highlights ==~~	+
-	~~<table><tr><td colspan='2'>[[4oyg~~]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OYG FirstGlance]. <br>	+
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASP:ASPARTIC+ACID'>ASP</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4oye\|4oye]]</td></tr>~~	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4oyg RCSB], [http://www.ebi.ac.uk/pdbsum/4oyg PDBsum]</span></td></tr>	+
-	~~</table>~~	+
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	~~Membrane transporters that clear the neurotransmitter glutamate from synapses are driven~~ by symport of sodium ions and counter-transport of a potassium ion. Previous crystal structures of a homologous archaeal sodium and aspartate symporter showed that a dedicated transport domain carries the substrate and ions across the membrane. Here, we report new crystal structures of this homologue in ligand-free and ions-only bound outward- and inward-facing conformations. We show that after ligand release, the apo transport domain adopts a compact and occluded conformation that can traverse the membrane, completing the transport cycle. Sodium binding primes the transport domain to accept its substrate and triggers extracellular gate opening, which prevents inward domain translocation until substrate binding takes place. Furthermore, we describe a new cation-binding site ideally suited to bind a counter-transported ion. We suggest that potassium binding at this site stabilizes the translocation-competent conformation of the unloaded transport domain in mammalian homologues.	+
-		+
-	Coupled ion binding and structural transitions along the transport cycle of glutamate transporters.,Verdon G, Oh S, Serio RN, Boudker O Elife. 2014 May 19:e02283. doi: 10.7554/eLife.02283. PMID:24842876<ref>PMID:24842876</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
-	~~__TOC__~~	+
-	~~</StructureSection>~~	+
-	~~[[Category: Boudker, O]]~~	+
-	~~[[Category: Oh, S]]~~	+
-	~~[[Category: Aspartate transporter]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Transport protein]]~~	+

OCA at 08:10, 20 January 2015

OCA — Tue, 20 Jan 2015 08:10:35 GMT

←Older revision		Revision as of 08:10, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4oyg]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OYG FirstGlance]. <br>		<table><tr><td colspan='2'>[[4oyg]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OYG FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASP:ASPARTIC+ACID'>ASP</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASP:ASPARTIC+ACID'>ASP</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4oye\|4oye]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4oye\|4oye]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4oyg RCSB], [http://www.ebi.ac.uk/pdbsum/4oyg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4oyg RCSB], [http://www.ebi.ac.uk/pdbsum/4oyg PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Boudker, O.]]	+	[[Category: Boudker, O]]
-	[[Category: Oh, S.]]	+	[[Category: Oh, S]]
	[[Category: Aspartate transporter]]		[[Category: Aspartate transporter]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 08:00, 18 June 2014

OCA — Wed, 18 Jun 2014 08:00:32 GMT

←Older revision		Revision as of 08:00, 18 June 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of GltPh R397A in complex with Na+ and L-asp==
		+	<StructureSection load='4oyg' size='340' side='right' caption='[[4oyg]], [[Resolution\|resolution]] 3.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4oyg]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OYG FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASP:ASPARTIC+ACID'>ASP</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4oye\|4oye]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4oyg RCSB], [http://www.ebi.ac.uk/pdbsum/4oyg PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Membrane transporters that clear the neurotransmitter glutamate from synapses are driven by symport of sodium ions and counter-transport of a potassium ion. Previous crystal structures of a homologous archaeal sodium and aspartate symporter showed that a dedicated transport domain carries the substrate and ions across the membrane. Here, we report new crystal structures of this homologue in ligand-free and ions-only bound outward- and inward-facing conformations. We show that after ligand release, the apo transport domain adopts a compact and occluded conformation that can traverse the membrane, completing the transport cycle. Sodium binding primes the transport domain to accept its substrate and triggers extracellular gate opening, which prevents inward domain translocation until substrate binding takes place. Furthermore, we describe a new cation-binding site ideally suited to bind a counter-transported ion. We suggest that potassium binding at this site stabilizes the translocation-competent conformation of the unloaded transport domain in mammalian homologues.

-	~~The entry 4oyg is ON HOLD~~	+	Coupled ion binding and structural transitions along the transport cycle of glutamate transporters.,Verdon G, Oh S, Serio RN, Boudker O Elife. 2014 May 19:e02283. doi: 10.7554/eLife.02283. PMID:24842876<ref>PMID:24842876</ref>

-	~~Authors~~: Boudker, O., Oh, S.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of GltPh R397A in complex with Na+ and L-asp~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Boudker, O.]]
		+	[[Category: Oh, S.]]
		+	[[Category: Aspartate transporter]]
		+	[[Category: Membrane protein]]
		+	[[Category: Transport protein]]

OCA at 07:05, 14 May 2014

OCA — Wed, 14 May 2014 07:05:57 GMT

←Older revision		Revision as of 07:05, 14 May 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4oyg is ON HOLD ~~until sometime in the future~~	+	The entry 4oyg is ON HOLD

	Authors: Boudker, O., Oh, S.		Authors: Boudker, O., Oh, S.

	Description: Crystal structure of GltPh R397A in complex with Na+ and L-asp		Description: Crystal structure of GltPh R397A in complex with Na+ and L-asp

OCA at 07:39, 7 May 2014

OCA — Wed, 07 May 2014 07:39:34 GMT

←Older revision		Revision as of 07:39, 7 May 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4oyg is ON HOLD	+	The entry 4oyg is ON HOLD until sometime in the future

	Authors: Boudker, O., Oh, S.		Authors: Boudker, O., Oh, S.

	Description: Crystal structure of GltPh R397A in complex with Na+ and L-asp		Description: Crystal structure of GltPh R397A in complex with Na+ and L-asp

OCA at 06:22, 30 April 2014

OCA — Wed, 30 Apr 2014 06:22:23 GMT

←Older revision		Revision as of 06:22, 30 April 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4oyg is ON HOLD ~~until sometime in the future~~	+	The entry 4oyg is ON HOLD

	Authors: Boudker, O., Oh, S.		Authors: Boudker, O., Oh, S.

	Description: Crystal structure of GltPh R397A in complex with Na+ and L-asp		Description: Crystal structure of GltPh R397A in complex with Na+ and L-asp

OCA at 09:01, 16 April 2014

OCA — Wed, 16 Apr 2014 09:01:33 GMT

←Older revision		Revision as of 09:01, 16 April 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4oyg is ON HOLD	+	The entry 4oyg is ON HOLD until sometime in the future

	Authors: Boudker, O., Oh, S.		Authors: Boudker, O., Oh, S.

	Description: Crystal structure of GltPh R397A in complex with Na+ and L-asp		Description: Crystal structure of GltPh R397A in complex with Na+ and L-asp

OCA at 07:29, 19 March 2014

OCA — Wed, 19 Mar 2014 07:29:43 GMT

←Older revision		Revision as of 07:29, 19 March 2014
Line 5:		Line 5:
	Authors: Boudker, O., Oh, S.		Authors: Boudker, O., Oh, S.

-	Description: Crystal structure of GltPh R397A in complex with Na+ and L-asp.	+	Description: Crystal structure of GltPh R397A in complex with Na+ and L-asp

OCA: Protected "4oyg" [edit=sysop:move=sysop]

OCA — Wed, 19 Feb 2014 16:26:11 GMT

Protected "4oyg" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:26, 19 February 2014

OCA: New page: '''Unreleased structure''' The entry 4oyg is ON HOLD Authors: Boudker, O., Oh, S. Description: Crystal structure of GltPh R397A in complex with Na+ and L-asp.

OCA — Wed, 19 Feb 2014 16:26:09 GMT

New page: '''Unreleased structure''' The entry 4oyg is ON HOLD Authors: Boudker, O., Oh, S. Description: Crystal structure of GltPh R397A in complex with Na+ and L-asp.

New page

'''Unreleased structure'''

The entry 4oyg is ON HOLD

Authors: Boudker, O., Oh, S.

Description: Crystal structure of GltPh R397A in complex with Na+ and L-asp.