4oyj - Revision history

OCA at 00:37, 28 December 2023

OCA — Thu, 28 Dec 2023 00:37:57 GMT

←Older revision		Revision as of 00:37, 28 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4oyj]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OYJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4oyj]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OYJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyj OCA], [https://pdbe.org/4oyj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oyj RCSB], [https://www.ebi.ac.uk/pdbsum/4oyj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oyj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyj OCA], [https://pdbe.org/4oyj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oyj RCSB], [https://www.ebi.ac.uk/pdbsum/4oyj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oyj ProSAT]</span></td></tr>
	</table>		</table>

OCA at 12:32, 1 February 2023

OCA — Wed, 01 Feb 2023 12:32:27 GMT

←Older revision		Revision as of 12:32, 1 February 2023
Line 3:		Line 3:
	<StructureSection load='4oyj' size='340' side='right'caption='[[4oyj]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='4oyj' size='340' side='right'caption='[[4oyj]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4oyj]] is a 13 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4OYJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4oyj]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OYJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RNF31,ZIBRA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyj OCA], [https://pdbe.org/4oyj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oyj RCSB], [https://www.ebi.ac.uk/pdbsum/4oyj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oyj ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4oyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyj OCA], [~~http~~://pdbe.org/4oyj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4oyj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4oyj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4oyj ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/RNF31_HUMAN RNF31_HUMAN]] E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('M-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types.<ref>PMID:17006537</ref> <ref>PMID:20005846</ref> <ref>PMID:19136968</ref> <ref>PMID:21455173</ref> <ref>PMID:21455180</ref> <ref>PMID:21455181</ref> <ref>PMID:22863777</ref>	+	[https://www.uniprot.org/uniprot/RNF31_HUMAN RNF31_HUMAN] E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('M-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types.<ref>PMID:17006537</ref> <ref>PMID:20005846</ref> <ref>PMID:19136968</ref> <ref>PMID:21455173</ref> <ref>PMID:21455180</ref> <ref>PMID:21455181</ref> <ref>PMID:22863777</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 20:

	==See Also==		==See Also==
-	*[[Ubiquitin protein ligase\|Ubiquitin protein ligase]]	+	*[[Ubiquitin protein ligase 3D structures\|Ubiquitin protein ligase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Elliott~~, P R~~]]	+	[[Category: Elliott PR]]
-	[[Category: Komander, D]]	+	[[Category: Komander D]]
-	~~[[Category: E3 ubiquitin ligase]]~~	+
-	~~[[Category: Ligase]]~~	+
-	~~[[Category: Pub domain~~]]	+

OCA at 07:19, 17 April 2019

OCA — Wed, 17 Apr 2019 07:19:18 GMT

←Older revision		Revision as of 07:19, 17 April 2019
Line 1:		Line 1:

	==Structure of the apo HOIP PUB domain==		==Structure of the apo HOIP PUB domain==
-	<StructureSection load='4oyj' size='340' side='right' caption='[[4oyj]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='4oyj' size='340' side='right'caption='[[4oyj]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4oyj]] is a 13 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OYJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4oyj]] is a 13 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OYJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RNF31,ZIBRA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyj OCA], [http://pdbe.org/4oyj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4oyj RCSB], [http://www.ebi.ac.uk/pdbsum/4oyj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4oyj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyj OCA], [http://pdbe.org/4oyj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4oyj RCSB], [http://www.ebi.ac.uk/pdbsum/4oyj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4oyj ProSAT]</span></td></tr>
	</table>		</table>
Line 25:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Elliott, P R]]		[[Category: Elliott, P R]]
	[[Category: Komander, D]]		[[Category: Komander, D]]

OCA at 05:19, 5 August 2016

OCA — Fri, 05 Aug 2016 05:19:21 GMT

←Older revision		Revision as of 05:19, 5 August 2016
Line 1:		Line 1:
		+
	==Structure of the apo HOIP PUB domain==		==Structure of the apo HOIP PUB domain==
	<StructureSection load='4oyj' size='340' side='right' caption='[[4oyj]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='4oyj' size='340' side='right' caption='[[4oyj]], [[Resolution\|resolution]] 3.00Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4oyj]] is a 13 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OYJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4oyj]] is a 13 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OYJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4oyj RCSB], [http://www.ebi.ac.uk/pdbsum/4oyj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyj OCA], [http://pdbe.org/4oyj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4oyj RCSB], [http://www.ebi.ac.uk/pdbsum/4oyj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4oyj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4oyj" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 23:19, 24 December 2014

OCA — Wed, 24 Dec 2014 23:19:22 GMT

←Older revision		Revision as of 23:19, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4oyj]] is a 13 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OYJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4oyj]] is a 13 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OYJ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4oyj RCSB], [http://www.ebi.ac.uk/pdbsum/4oyj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4oyj RCSB], [http://www.ebi.ac.uk/pdbsum/4oyj PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RNF31_HUMAN RNF31_HUMAN]] E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('M-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types.<ref>PMID:17006537</ref> <ref>PMID:20005846</ref> <ref>PMID:19136968</ref> <ref>PMID:21455173</ref> <ref>PMID:21455180</ref> <ref>PMID:21455181</ref> <ref>PMID:22863777</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Elliott, P R.]]	+	[[Category: Elliott, P R]]
-	[[Category: Komander, D.]]	+	[[Category: Komander, D]]
	[[Category: E3 ubiquitin ligase]]		[[Category: E3 ubiquitin ligase]]
	[[Category: Ligase]]		[[Category: Ligase]]
	[[Category: Pub domain]]		[[Category: Pub domain]]

OCA at 06:25, 11 June 2014

OCA — Wed, 11 Jun 2014 06:25:23 GMT

←Older revision		Revision as of 06:25, 11 June 2014
Line 12:		Line 12:
	Molecular Basis and Regulation of OTULIN-LUBAC Interaction.,Elliott PR, Nielsen SV, Marco-Casanova P, Fiil BK, Keusekotten K, Mailand N, Freund SM, Gyrd-Hansen M, Komander D Mol Cell. 2014 May 8;54(3):335-48. doi: 10.1016/j.molcel.2014.03.018. Epub 2014, Apr 10. PMID:24726323<ref>PMID:24726323</ref>		Molecular Basis and Regulation of OTULIN-LUBAC Interaction.,Elliott PR, Nielsen SV, Marco-Casanova P, Fiil BK, Keusekotten K, Mailand N, Freund SM, Gyrd-Hansen M, Komander D Mol Cell. 2014 May 8;54(3):335-48. doi: 10.1016/j.molcel.2014.03.018. Epub 2014, Apr 10. PMID:24726323<ref>PMID:24726323</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[Ubiquitin protein ligase\|Ubiquitin protein ligase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:59, 21 May 2014

OCA — Wed, 21 May 2014 08:59:30 GMT

←Older revision		Revision as of 08:59, 21 May 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Structure of the apo HOIP PUB domain==
		+	<StructureSection load='4oyj' size='340' side='right' caption='[[4oyj]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4oyj]] is a 13 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OYJ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oyj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4oyj RCSB], [http://www.ebi.ac.uk/pdbsum/4oyj PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The linear ubiquitin (Ub) chain assembly complex (LUBAC) generates Met1-linked "linear" Ub chains that regulate the activation of the nuclear factor kappaB (NFkappaB) transcription factor and other processes. We recently discovered OTULIN as a deubiquitinase that specifically cleaves Met1-linked polyUb. Now, we show that OTULIN binds via a conserved PUB-interacting motif (PIM) to the PUB domain of the LUBAC component HOIP. Crystal structures and nuclear magnetic resonance experiments reveal the molecular basis for the high-affinity interaction and explain why OTULIN binds the HOIP PUB domain specifically. Analysis of LUBAC-induced NFkappaB signaling suggests that OTULIN needs to be present on LUBAC in order to restrict Met1-polyUb signaling. Moreover, LUBAC-OTULIN complex formation is regulated by OTULIN phosphorylation in the PIM. Phosphorylation of OTULIN prevents HOIP binding, whereas unphosphorylated OTULIN is part of the endogenous LUBAC complex. Our work exemplifies how coordination of ubiquitin assembly and disassembly activities in protein complexes regulates individual Ub linkage types.

-	~~The entry 4oyj is ON HOLD until Paper Publication~~	+	Molecular Basis and Regulation of OTULIN-LUBAC Interaction.,Elliott PR, Nielsen SV, Marco-Casanova P, Fiil BK, Keusekotten K, Mailand N, Freund SM, Gyrd-Hansen M, Komander D Mol Cell. 2014 May 8;54(3):335-48. doi: 10.1016/j.molcel.2014.03.018. Epub 2014, Apr 10. PMID:24726323<ref>PMID:24726323</ref>

-	~~Authors~~: Elliott, P.R., Komander, D.	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Structure of the apo HOIP PUB~~ domain	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Elliott, P R.]]
		+	[[Category: Komander, D.]]
		+	[[Category: E3 ubiquitin ligase]]
		+	[[Category: Ligase]]
		+	[[Category: Pub domain]]

OCA at 06:22, 30 April 2014

OCA — Wed, 30 Apr 2014 06:22:27 GMT

←Older revision		Revision as of 06:22, 30 April 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4oyj is ON HOLD	+	The entry 4oyj is ON HOLD until Paper Publication

	Authors: Elliott, P.R., Komander, D.		Authors: Elliott, P.R., Komander, D.

	Description: Structure of the apo HOIP PUB domain		Description: Structure of the apo HOIP PUB domain

OCA: Protected "4oyj" [edit=sysop:move=sysop]

OCA — Wed, 19 Feb 2014 16:26:19 GMT

Protected "4oyj" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:26, 19 February 2014

OCA: New page: '''Unreleased structure''' The entry 4oyj is ON HOLD Authors: Elliott, P.R., Komander, D. Description: Structure of the apo HOIP PUB domain

OCA — Wed, 19 Feb 2014 16:26:18 GMT

New page: '''Unreleased structure''' The entry 4oyj is ON HOLD Authors: Elliott, P.R., Komander, D. Description: Structure of the apo HOIP PUB domain

New page

'''Unreleased structure'''

The entry 4oyj is ON HOLD

Authors: Elliott, P.R., Komander, D.

Description: Structure of the apo HOIP PUB domain