4oyy - Revision history

OCA at 00:38, 28 December 2023

OCA — Thu, 28 Dec 2023 00:38:07 GMT

←Older revision		Revision as of 00:38, 28 December 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4oyy]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OYY FirstGlance]. <br>		<table><tr><td colspan='2'>[[4oyy]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OYY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyy OCA], [https://pdbe.org/4oyy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oyy RCSB], [https://www.ebi.ac.uk/pdbsum/4oyy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oyy ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyy OCA], [https://pdbe.org/4oyy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oyy RCSB], [https://www.ebi.ac.uk/pdbsum/4oyy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oyy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 12:33, 1 February 2023

OCA — Wed, 01 Feb 2023 12:33:00 GMT

←Older revision		Revision as of 12:33, 1 February 2023
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	<StructureSection load='4oyy' size='340' side='right'caption='[[4oyy]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='4oyy' size='340' side='right'caption='[[4oyy]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4oyy]] is a 12 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_16454 Atcc 16454~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4OYY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4oyy]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Humicola_insolens Humicola insolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OYY FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4oyl\|4oyl]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyy OCA], [https://pdbe.org/4oyy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oyy RCSB], [https://www.ebi.ac.uk/pdbsum/4oyy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oyy ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4oyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyy OCA], [~~http~~://pdbe.org/4oyy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4oyy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4oyy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4oyy ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A075B5G4_HUMIN A0A075B5G4_HUMIN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 16454~~]]	+	[[Category: Humicola insolens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Brzozowski~~, A M~~]]	+	[[Category: Brzozowski AM]]
-	[[Category: Dauter~~, Z D~~]]	+	[[Category: Dauter ZD]]
-	[[Category: Turkenburg~~, J P~~]]	+	[[Category: Turkenburg JP]]
-	[[Category: Wilson~~, K S]]~~	+	[[Category: Wilson KS]]
-	~~[[Category: Hydrolase~~]]	+

OCA at 08:04, 19 February 2020

OCA — Wed, 19 Feb 2020 08:04:01 GMT

←Older revision		Revision as of 08:04, 19 February 2020
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	==Humicola insolens cutinase==		==Humicola insolens cutinase==
-	<StructureSection load='4oyy' size='340' side='right' caption='[[4oyy]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='4oyy' size='340' side='right'caption='[[4oyy]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4oyy]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_16454 Atcc 16454]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OYY FirstGlance]. <br>		<table><tr><td colspan='2'>[[4oyy]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_16454 Atcc 16454]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OYY FirstGlance]. <br>
Line 18:		Line 18:

	==See Also==		==See Also==
-	*[[Cutinase\|Cutinase]]	+	*[[Cutinase 3D structures\|Cutinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Atcc 16454]]		[[Category: Atcc 16454]]
		+	[[Category: Large Structures]]
	[[Category: Brzozowski, A M]]		[[Category: Brzozowski, A M]]
	[[Category: Dauter, Z D]]		[[Category: Dauter, Z D]]

OCA at 16:05, 16 November 2017

OCA — Thu, 16 Nov 2017 16:05:50 GMT

←Older revision		Revision as of 16:05, 16 November 2017
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		+
	==Humicola insolens cutinase==		==Humicola insolens cutinase==
	<StructureSection load='4oyy' size='340' side='right' caption='[[4oyy]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='4oyy' size='340' side='right' caption='[[4oyy]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4oyy]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OYY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4oyy]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_16454 Atcc 16454]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OYY FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4oyl\|4oyl]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4oyl\|4oyl]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyy OCA], [http://pdbe.org/4oyy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4oyy RCSB], [http://www.ebi.ac.uk/pdbsum/4oyy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyy OCA], [http://pdbe.org/4oyy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4oyy RCSB], [http://www.ebi.ac.uk/pdbsum/4oyy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4oyy ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 16454]]
	[[Category: Brzozowski, A M]]		[[Category: Brzozowski, A M]]
	[[Category: Dauter, Z D]]		[[Category: Dauter, Z D]]

OCA at 06:55, 30 September 2015

OCA — Wed, 30 Sep 2015 06:55:31 GMT

←Older revision		Revision as of 06:55, 30 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[4oyy]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OYY FirstGlance]. <br>		<table><tr><td colspan='2'>[[4oyy]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OYY FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4oyl\|4oyl]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4oyl\|4oyl]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4oyy RCSB], [http://www.ebi.ac.uk/pdbsum/4oyy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyy OCA], [http://pdbe.org/4oyy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4oyy RCSB], [http://www.ebi.ac.uk/pdbsum/4oyy PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 14:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4oyy" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cutinase\|Cutinase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:43, 5 January 2015

OCA — Mon, 05 Jan 2015 12:43:57 GMT

←Older revision		Revision as of 12:43, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4oyy]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OYY FirstGlance]. <br>		<table><tr><td colspan='2'>[[4oyy]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OYY FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4oyl\|4oyl]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4oyl\|4oyl]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4oyy RCSB], [http://www.ebi.ac.uk/pdbsum/4oyy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4oyy RCSB], [http://www.ebi.ac.uk/pdbsum/4oyy PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Brzozowski, A M.]]	+	[[Category: Brzozowski, A M]]
-	[[Category: Dauter, Z D.]]	+	[[Category: Dauter, Z D]]
-	[[Category: Turkenburg, J P.]]	+	[[Category: Turkenburg, J P]]
-	[[Category: Wilson, K S.]]	+	[[Category: Wilson, K S]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 07:38, 30 July 2014

OCA — Wed, 30 Jul 2014 07:38:55 GMT

←Older revision		Revision as of 07:38, 30 July 2014
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-	'''~~Unreleased structure~~'''	+	==Humicola insolens cutinase==
		+	<StructureSection load='4oyy' size='340' side='right' caption='[[4oyy]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4oyy]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OYY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OYY FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4oyl\|4oyl]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oyy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4oyy RCSB], [http://www.ebi.ac.uk/pdbsum/4oyy PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The interaction of lipolytic enzymes with anionic surfactants is of great interest with respect to industrially produced detergents. Here, we report the interaction of cutinase from the thermophilic fungus Humicola insolens with the anionic surfactant SDS, and show the enzyme specifically binds a single SDS molecule under nondenaturing concentrations. Protein interaction with SDS was investigated by NMR, ITC and molecular dynamics simulations. The NMR resonances of the protein were assigned, with large stretches of the protein molecule not showing any detectable resonances. SDS is shown to specifically interact with the loops surrounding the catalytic triad with medium affinity (Ka approximately 105 M-1 ). The mode of binding is closely similar to that seen previously for binding of amphiphilic molecules and substrate analogues to cutinases, and hence SDS acts as a substrate mimic. In addition, the structure of the enzyme has been solved by X-ray crystallography in its apo form and after cocrystallization with diethyl p-nitrophenyl phosphate (DNPP) leading to a complex with monoethylphosphate (MEP) esterified to the catalytically active serine. The enzyme has the same fold as reported for other cutinases but, unexpectedly, esterification of the active site serine is accompanied by the ethylation of the active site histidine which flips out from its usual position in the triad.

-	~~The entry 4oyy is ON HOLD until Paper Publication~~	+	Thermodynamic and structural investigation of the specific SDS binding of humicola insolens cutinase.,Kold D, Dauter Z, Laustsen AK, Brzozowski AM, Turkenburg JP, Nielsen AD, Koldso H, Petersen E, Schiott B, De Maria L, Wilson KS, Svendsen A, Wimmer R Protein Sci. 2014 May 16. doi: 10.1002/pro.2489. PMID:24832484<ref>PMID:24832484</ref>

-	~~Authors: Dauter~~, Z.D., Brzozowski, A.M., Turkenburg, J.P., Wilson, K.S.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Humicola insolens cutinase~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Brzozowski, A M.]]
		+	[[Category: Dauter, Z D.]]
		+	[[Category: Turkenburg, J P.]]
		+	[[Category: Wilson, K S.]]
		+	[[Category: Hydrolase]]

OCA at 07:00, 23 July 2014

OCA — Wed, 23 Jul 2014 07:00:40 GMT

←Older revision		Revision as of 07:00, 23 July 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4oyy is ON HOLD	+	The entry 4oyy is ON HOLD until Paper Publication

	Authors: Dauter, Z.D., Brzozowski, A.M., Turkenburg, J.P., Wilson, K.S.		Authors: Dauter, Z.D., Brzozowski, A.M., Turkenburg, J.P., Wilson, K.S.

	Description: Humicola insolens cutinase		Description: Humicola insolens cutinase

OCA at 12:24, 12 March 2014

OCA — Wed, 12 Mar 2014 12:24:50 GMT

←Older revision		Revision as of 12:24, 12 March 2014
Line 3:		Line 3:
	The entry 4oyy is ON HOLD		The entry 4oyy is ON HOLD

-	Authors: Dauter, Z.D. Brzozowski, A.M. Turkenburg, J.P. Wilson, K.S.	+	Authors: Dauter, Z.D., Brzozowski, A.M., Turkenburg, J.P., Wilson, K.S.

	Description: Humicola insolens cutinase		Description: Humicola insolens cutinase

OCA: Protected "4oyy" [edit=sysop:move=sysop]

OCA — Wed, 19 Feb 2014 16:26:57 GMT

Protected "4oyy" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:26, 19 February 2014