4p9n - Revision history

OCA at 00:41, 28 December 2023

OCA — Thu, 28 Dec 2023 00:41:45 GMT

←Older revision		Revision as of 00:41, 28 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4p9n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_shibatae Saccharolobus shibatae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P9N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4P9N FirstGlance]. <br>		<table><tr><td colspan='2'>[[4p9n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_shibatae Saccharolobus shibatae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P9N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4P9N FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4p9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p9n OCA], [https://pdbe.org/4p9n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4p9n RCSB], [https://www.ebi.ac.uk/pdbsum/4p9n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4p9n ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4p9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p9n OCA], [https://pdbe.org/4p9n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4p9n RCSB], [https://www.ebi.ac.uk/pdbsum/4p9n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4p9n ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 12:48, 1 February 2023

OCA — Wed, 01 Feb 2023 12:48:39 GMT

←Older revision		Revision as of 12:48, 1 February 2023
Line 3:		Line 3:
	<StructureSection load='4p9n' size='340' side='right'caption='[[4p9n]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='4p9n' size='340' side='right'caption='[[4p9n]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4p9n]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_51178 Atcc 51178~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P9N OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4P9N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4p9n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_shibatae Saccharolobus shibatae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P9N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4P9N FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SshEstI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2286 ATCC 51178])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4p9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p9n OCA], [https://pdbe.org/4p9n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4p9n RCSB], [https://www.ebi.ac.uk/pdbsum/4p9n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4p9n ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4p9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p9n OCA], [~~http~~://pdbe.org/4p9n PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4p9n RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4p9n PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4p9n ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q5NU42_SACSH Q5NU42_SACSH]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 19:

	==See Also==		==See Also==
-	*[[Carboxylesterase\|Carboxylesterase]]	+	*[[Carboxylesterase 3D structures\|Carboxylesterase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 51178]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Unno, H~~]]	+	[[Category: Saccharolobus shibatae]]
-	[[Category: ~~Alpha/beta-hydrorase fold]]~~	+	[[Category: Unno H]]
-	~~[[Category: Carboxylesterase]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 07:49, 17 April 2019

OCA — Wed, 17 Apr 2019 07:49:08 GMT

←Older revision		Revision as of 07:49, 17 April 2019
Line 1:		Line 1:

	==Crystal structure of sshesti PE mutant==		==Crystal structure of sshesti PE mutant==
-	<StructureSection load='4p9n' size='340' side='right' caption='[[4p9n]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='4p9n' size='340' side='right'caption='[[4p9n]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4p9n]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P9N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P9N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4p9n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_51178 Atcc 51178]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P9N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P9N FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p9n OCA], [http://pdbe.org/4p9n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4p9n RCSB], [http://www.ebi.ac.uk/pdbsum/4p9n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4p9n ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SshEstI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2286 ATCC 51178])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p9n OCA], [http://pdbe.org/4p9n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4p9n RCSB], [http://www.ebi.ac.uk/pdbsum/4p9n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4p9n ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 51178]]
		+	[[Category: Large Structures]]
	[[Category: Unno, H]]		[[Category: Unno, H]]
	[[Category: Alpha/beta-hydrorase fold]]		[[Category: Alpha/beta-hydrorase fold]]
	[[Category: Carboxylesterase]]		[[Category: Carboxylesterase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 17:13, 11 August 2016

OCA — Thu, 11 Aug 2016 17:13:21 GMT

←Older revision		Revision as of 17:13, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of sshesti PE mutant==		==Crystal structure of sshesti PE mutant==
	<StructureSection load='4p9n' size='340' side='right' caption='[[4p9n]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='4p9n' size='340' side='right' caption='[[4p9n]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4p9n]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P9N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P9N FirstGlance]. <br>		<table><tr><td colspan='2'>[[4p9n]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P9N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P9N FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p9n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4p9n RCSB], [http://www.ebi.ac.uk/pdbsum/4p9n PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p9n OCA], [http://pdbe.org/4p9n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4p9n RCSB], [http://www.ebi.ac.uk/pdbsum/4p9n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4p9n ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 13:		Line 14:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4p9n" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Carboxylesterase\|Carboxylesterase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:30, 20 January 2015

OCA — Tue, 20 Jan 2015 11:30:27 GMT

←Older revision		Revision as of 11:30, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4p9n]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P9N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P9N FirstGlance]. <br>		<table><tr><td colspan='2'>[[4p9n]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P9N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P9N FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p9n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4p9n RCSB], [http://www.ebi.ac.uk/pdbsum/4p9n PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p9n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4p9n RCSB], [http://www.ebi.ac.uk/pdbsum/4p9n PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 17:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Unno, H.]]	+	[[Category: Unno, H]]
	[[Category: Alpha/beta-hydrorase fold]]		[[Category: Alpha/beta-hydrorase fold]]
	[[Category: Carboxylesterase]]		[[Category: Carboxylesterase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 06:20, 13 August 2014

OCA — Wed, 13 Aug 2014 06:20:05 GMT

←Older revision		Revision as of 06:20, 13 August 2014
Line 5:		Line 5:
	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p9n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4p9n RCSB], [http://www.ebi.ac.uk/pdbsum/4p9n PDBsum]</span></td></tr>		</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p9n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4p9n RCSB], [http://www.ebi.ac.uk/pdbsum/4p9n PDBsum]</span></td></tr>
	<table>		<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	To investigate the mechanism for low-pH adaptation by a carboxylesterase, structural and biochemical analyses of EstFa_R (a recombinant, slightly acidophilic carboxylesterase from Ferroplasma acidiphilum) and SshEstI (an alkaliphilic carboxylesterase from Sulfolobus shibatae DSM5389) were performed. Although a previous proteomic study by another group showed that the enzyme purified from F. acidiphilum contained an iron atom, EstFa_R did not bind to iron, as analyzed by ICP-MS and isothermal titration calorimetry. The crystal structures of EstFa_R and SshEstI were determined at 1.6A and 1.5A resolutions, respectively. EstFa_R had a catalytic triad with an extended hydrogen bond network that was not observed in SshEstI. Quadruple mutants of both proteins were created to remove or introduce the extended hydrogen bond network. The mutation on EstFa_R enhanced its catalytic efficiency and gave it an alkaline pH optimum, whereas the mutation on SshEstI resulted in opposite effects (i.e., decrease in the catalytic efficiency and a downward shift in the optimum pH). Our experimental results suggest that the low pH optimum of EstFa_R activity was a result of the unique extended hydrogen bond network in the catalytic triad and the highly negative charged surface around the active site. The change in the pH optimum of EstFa_R happened simultaneously with a change in the catalytic efficiency, suggesting that the local flexibility of the active site in EstFa_R could be modified by quadruple mutation. These observations may provide a novel strategy to elucidate the low-pH adaptation of serine hydrolases.
		+
		+	Structural Insights into the Low-pH Adaptation of a Unique Carboxylesterase from Ferroplasma: Altering the pH Optima of Two Carboxylesterases.,Ohara K, Unno H, Oshima Y, Hosoya M, Fujino N, Hirooka K, Takahashi S, Yamashita S, Kusunoki M, Nakayama T J Biol Chem. 2014 Jul 20. pii: jbc.M113.521856. PMID:25043762<ref>PMID:25043762</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:50, 30 July 2014

OCA — Wed, 30 Jul 2014 07:50:37 GMT

←Older revision		Revision as of 07:50, 30 July 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal structure of sshesti PE mutant==
-		+	<StructureSection load='4p9n' size='340' side='right' caption='[[4p9n]], [[Resolution\|resolution]] 1.80Å' scene=''>
-	~~The entry~~ 4p9n is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4p9n]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P9N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P9N FirstGlance]. <br>
-	~~Authors~~: Unno, H.	+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p9n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p9n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4p9n RCSB], [http://www.ebi.ac.uk/pdbsum/4p9n PDBsum]</span></td></tr>
-		+	<table>
-	~~Description~~: ~~Crystal structure of sshesti PE mutant~~	+	__TOC__
		+	</StructureSection>
		+	[[Category: Unno, H.]]
		+	[[Category: Alpha/beta-hydrorase fold]]
		+	[[Category: Carboxylesterase]]
		+	[[Category: Hydrolase]]

OCA at 05:45, 25 June 2014

OCA — Wed, 25 Jun 2014 05:45:03 GMT

←Older revision		Revision as of 05:45, 25 June 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4p9n is ON HOLD	+	The entry 4p9n is ON HOLD until Paper Publication

	Authors: Unno, H.		Authors: Unno, H.

	Description: Crystal structure of sshesti PE mutant		Description: Crystal structure of sshesti PE mutant

OCA at 07:23, 23 April 2014

OCA — Wed, 23 Apr 2014 07:23:21 GMT

←Older revision		Revision as of 07:23, 23 April 2014
Line 3:		Line 3:
	The entry 4p9n is ON HOLD		The entry 4p9n is ON HOLD

-	Authors: Unno, H	+	Authors: Unno, H.

-	Description: ~~CRYSTAL STRUCTURE OF SSHESTI~~ PE ~~MUTANT~~	+	Description: Crystal structure of sshesti PE mutant

OCA: Protected "4p9n" [edit=sysop:move=sysop]

OCA — Wed, 09 Apr 2014 05:55:12 GMT

Protected "4p9n" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:55, 9 April 2014