4pcg - Revision history

OCA at 10:16, 27 March 2024

OCA — Wed, 27 Mar 2024 10:16:15 GMT

←Older revision		Revision as of 10:16, 27 March 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/D6QWI0_9POLY D6QWI0_9POLY]		[https://www.uniprot.org/uniprot/D6QWI0_9POLY D6QWI0_9POLY]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Human polyomaviruses 6 and 7 (HPyV6 and HPyV7, respectively) are commonly found on human skin. We have determined X-ray structures of their major capsid proteins, VP1 at resolutions of 1.8 and 1.7 A, respectively. In polyomaviruses, VP1 commonly determines antigenicity as well as cell-surface receptor specificity, and the protein is therefore linked to attachment, tropism, and ultimately viral pathogenicity. The structures of HPyV6 and HPyV7 VP1 reveal uniquely elongated loops that cover the bulk of the outer virion surfaces, obstructing a groove that binds sialylated glycan receptors in many other polyomaviruses. In support of this structural observation, interactions of VP1 with alpha2,3- and alpha2,6-linked sialic acids could not be detected in solution by Nuclear Magnetic Resonance spectroscopy. Single-cell binding studies indicate that sialylated glycans are likely not required for initial attachment to cultured human cells. Our findings establish distinct antigenic properties of HPyV6 and HPyV7 capsids and indicate that these two viruses engage non-sialylated receptors. IMPORTANCE 150: Eleven new human polyomaviruses, including the skin viruses HPyV6 and HPyV7, have been identified during the last decade. In contrast to better-studied polyomaviruses, routes of infection, cell tropism and entry pathways of many of these new viruses remain largely mysterious. Our high-resolution X-ray structures of major capsid proteins VP1 from HPyV6 and HPyV7 reveal critical differences in surface morphology in comparison to all other known polyomavirus structures. A groove that engages specific sialic acid-containing glycan receptors in related polyomaviruses is obstructed and HPyV6 and HPyV7 VP1 do not interact with sialylated compounds in solution or on cultured human cells. A comprehensive comparison with other structurally characterized polyomavirus VP1 proteins enhances our understanding of molecular determinants that underlie receptor-specificity, antigenicity and ultimately pathogenicity within the polyomavirus family and highlight the need for structure-based analysis to better define phylogenetic relationships within the growing polyomavirus family and perhaps also other viruses.
-
-	Structure Analysis of the Major Capsid Proteins of the Human Polyomavirus 6 and 7 Reveals an Obstructed Sialic Acid Binding Site.,Stroh LJ, Neu U, Blaum BS, Buch MH, Garcea RL, Stehle T J Virol. 2014 Jul 9. pii: JVI.01084-14. PMID:25008942<ref>PMID:25008942</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4pcg" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Virus coat proteins 3D structures\|Virus coat proteins 3D structures]]		*[[Virus coat proteins 3D structures\|Virus coat proteins 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 00:42, 28 December 2023

OCA — Thu, 28 Dec 2023 00:42:38 GMT

←Older revision		Revision as of 00:42, 28 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4pcg]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_polyomavirus_6 Human polyomavirus 6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PCG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PCG FirstGlance]. <br>		<table><tr><td colspan='2'>[[4pcg]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_polyomavirus_6 Human polyomavirus 6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PCG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PCG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pcg OCA], [https://pdbe.org/4pcg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pcg RCSB], [https://www.ebi.ac.uk/pdbsum/4pcg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pcg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pcg OCA], [https://pdbe.org/4pcg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pcg RCSB], [https://www.ebi.ac.uk/pdbsum/4pcg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pcg ProSAT]</span></td></tr>
	</table>		</table>

OCA at 12:52, 1 February 2023

OCA — Wed, 01 Feb 2023 12:52:16 GMT

←Older revision		Revision as of 12:52, 1 February 2023
Line 3:		Line 3:
	<StructureSection load='4pcg' size='340' side='right'caption='[[4pcg]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='4pcg' size='340' side='right'caption='[[4pcg]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4pcg]] is a 5 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human_polyomavirus_6 Human polyomavirus 6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PCG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4PCG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4pcg]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_polyomavirus_6 Human polyomavirus 6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PCG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PCG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pch\|4pch]]~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pcg OCA], [https://pdbe.org/4pcg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pcg RCSB], [https://www.ebi.ac.uk/pdbsum/4pcg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pcg ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4pcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pcg OCA], [~~http~~://pdbe.org/4pcg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4pcg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4pcg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4pcg ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/D6QWI0_9POLY D6QWI0_9POLY]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 4pcg" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4pcg" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Virus coat proteins 3D structures\|Virus coat proteins 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 23:		Line 27:
	[[Category: Human polyomavirus 6]]		[[Category: Human polyomavirus 6]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Stehle, T]]	+	[[Category: Stehle T]]
-	[[Category: Stroh~~, L J]]~~	+	[[Category: Stroh LJ]]
-	~~[[Category: Attachment to host-cell receptor]]~~	+
-	~~[[Category: Jelly-roll topology]]~~	+
-	~~[[Category: Major viral capsid protein]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 07:51, 19 February 2020

OCA — Wed, 19 Feb 2020 07:51:42 GMT

←Older revision		Revision as of 07:51, 19 February 2020
Line 1:		Line 1:

	==Structure of Human Polyomavirus 6 (HPyV6) VP1 pentamer==		==Structure of Human Polyomavirus 6 (HPyV6) VP1 pentamer==
-	<StructureSection load='4pcg' size='340' side='right' caption='[[4pcg]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='4pcg' size='340' side='right'caption='[[4pcg]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4pcg]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_polyomavirus_6 Human polyomavirus 6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PCG FirstGlance]. <br>		<table><tr><td colspan='2'>[[4pcg]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_polyomavirus_6 Human polyomavirus 6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PCG FirstGlance]. <br>
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Human polyomavirus 6]]		[[Category: Human polyomavirus 6]]
		+	[[Category: Large Structures]]
	[[Category: Stehle, T]]		[[Category: Stehle, T]]
	[[Category: Stroh, L J]]		[[Category: Stroh, L J]]

OCA at 08:37, 22 November 2017

OCA — Wed, 22 Nov 2017 08:37:37 GMT

←Older revision		Revision as of 08:37, 22 November 2017
Line 3:		Line 3:
	<StructureSection load='4pcg' size='340' side='right' caption='[[4pcg]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='4pcg' size='340' side='right' caption='[[4pcg]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4pcg]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PCG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4pcg]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_polyomavirus_6 Human polyomavirus 6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PCG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pch\|4pch]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pch\|4pch]]</td></tr>
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human polyomavirus 6]]
	[[Category: Stehle, T]]		[[Category: Stehle, T]]
	[[Category: Stroh, L J]]		[[Category: Stroh, L J]]

OCA at 16:46, 11 August 2016

OCA — Thu, 11 Aug 2016 16:46:50 GMT

←Older revision		Revision as of 16:46, 11 August 2016
Line 1:		Line 1:
		+
	==Structure of Human Polyomavirus 6 (HPyV6) VP1 pentamer==		==Structure of Human Polyomavirus 6 (HPyV6) VP1 pentamer==
	<StructureSection load='4pcg' size='340' side='right' caption='[[4pcg]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='4pcg' size='340' side='right' caption='[[4pcg]], [[Resolution\|resolution]] 1.80Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pch\|4pch]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pch\|4pch]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pcg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pcg RCSB], [http://www.ebi.ac.uk/pdbsum/4pcg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pcg OCA], [http://pdbe.org/4pcg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pcg RCSB], [http://www.ebi.ac.uk/pdbsum/4pcg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pcg ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4pcg" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:08, 5 January 2015

OCA — Mon, 05 Jan 2015 14:08:18 GMT

←Older revision		Revision as of 14:08, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4pcg]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PCG FirstGlance]. <br>		<table><tr><td colspan='2'>[[4pcg]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PCG FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pch\|4pch]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pch\|4pch]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pcg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pcg RCSB], [http://www.ebi.ac.uk/pdbsum/4pcg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pcg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pcg RCSB], [http://www.ebi.ac.uk/pdbsum/4pcg PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Stehle, T.]]	+	[[Category: Stehle, T]]
-	[[Category: Stroh, L J.]]	+	[[Category: Stroh, L J]]
	[[Category: Attachment to host-cell receptor]]		[[Category: Attachment to host-cell receptor]]
	[[Category: Jelly-roll topology]]		[[Category: Jelly-roll topology]]
	[[Category: Major viral capsid protein]]		[[Category: Major viral capsid protein]]
	[[Category: Viral protein]]		[[Category: Viral protein]]

OCA at 02:19, 7 August 2014

OCA — Thu, 07 Aug 2014 02:19:29 GMT

←Older revision		Revision as of 02:19, 7 August 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Structure of Human Polyomavirus 6 (HPyV6) VP1 pentamer==
		+	<StructureSection load='4pcg' size='340' side='right' caption='[[4pcg]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4pcg]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PCG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PCG FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pch\|4pch]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pcg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pcg RCSB], [http://www.ebi.ac.uk/pdbsum/4pcg PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Human polyomaviruses 6 and 7 (HPyV6 and HPyV7, respectively) are commonly found on human skin. We have determined X-ray structures of their major capsid proteins, VP1 at resolutions of 1.8 and 1.7 A, respectively. In polyomaviruses, VP1 commonly determines antigenicity as well as cell-surface receptor specificity, and the protein is therefore linked to attachment, tropism, and ultimately viral pathogenicity. The structures of HPyV6 and HPyV7 VP1 reveal uniquely elongated loops that cover the bulk of the outer virion surfaces, obstructing a groove that binds sialylated glycan receptors in many other polyomaviruses. In support of this structural observation, interactions of VP1 with alpha2,3- and alpha2,6-linked sialic acids could not be detected in solution by Nuclear Magnetic Resonance spectroscopy. Single-cell binding studies indicate that sialylated glycans are likely not required for initial attachment to cultured human cells. Our findings establish distinct antigenic properties of HPyV6 and HPyV7 capsids and indicate that these two viruses engage non-sialylated receptors. IMPORTANCE 150: Eleven new human polyomaviruses, including the skin viruses HPyV6 and HPyV7, have been identified during the last decade. In contrast to better-studied polyomaviruses, routes of infection, cell tropism and entry pathways of many of these new viruses remain largely mysterious. Our high-resolution X-ray structures of major capsid proteins VP1 from HPyV6 and HPyV7 reveal critical differences in surface morphology in comparison to all other known polyomavirus structures. A groove that engages specific sialic acid-containing glycan receptors in related polyomaviruses is obstructed and HPyV6 and HPyV7 VP1 do not interact with sialylated compounds in solution or on cultured human cells. A comprehensive comparison with other structurally characterized polyomavirus VP1 proteins enhances our understanding of molecular determinants that underlie receptor-specificity, antigenicity and ultimately pathogenicity within the polyomavirus family and highlight the need for structure-based analysis to better define phylogenetic relationships within the growing polyomavirus family and perhaps also other viruses.

-	~~The entry 4pcg is ON HOLD until Paper Publication~~	+	Structure Analysis of the Major Capsid Proteins of the Human Polyomavirus 6 and 7 Reveals an Obstructed Sialic Acid Binding Site.,Stroh LJ, Neu U, Blaum BS, Buch MH, Garcea RL, Stehle T J Virol. 2014 Jul 9. pii: JVI.01084-14. PMID:25008942<ref>PMID:25008942</ref>

-	~~Authors: Stroh~~, L.J., Stehle, T.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~:	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Stehle, T.]]
		+	[[Category: Stroh, L J.]]
		+	[[Category: Attachment to host-cell receptor]]
		+	[[Category: Jelly-roll topology]]
		+	[[Category: Major viral capsid protein]]
		+	[[Category: Viral protein]]

OCA at 08:01, 21 May 2014

OCA — Wed, 21 May 2014 08:01:37 GMT

←Older revision		Revision as of 08:01, 21 May 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4pcg is ON HOLD	+	The entry 4pcg is ON HOLD until Paper Publication

	Authors: Stroh, L.J., Stehle, T.		Authors: Stroh, L.J., Stehle, T.

	Description:		Description:

OCA: Protected "4pcg" [edit=sysop:move=sysop]

OCA — Wed, 23 Apr 2014 07:25:46 GMT

Protected "4pcg" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:25, 23 April 2014