4pic - Revision history

OCA at 00:44, 28 December 2023

OCA — Thu, 28 Dec 2023 00:44:15 GMT

←Older revision		Revision as of 00:44, 28 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4pic]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PIC FirstGlance]. <br>		<table><tr><td colspan='2'>[[4pic]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PIC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pic OCA], [https://pdbe.org/4pic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pic RCSB], [https://www.ebi.ac.uk/pdbsum/4pic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pic ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pic OCA], [https://pdbe.org/4pic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pic RCSB], [https://www.ebi.ac.uk/pdbsum/4pic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pic ProSAT]</span></td></tr>
	</table>		</table>

OCA at 13:05, 1 February 2023

OCA — Wed, 01 Feb 2023 13:05:24 GMT

←Older revision		Revision as of 13:05, 1 February 2023
Line 1:		Line 1:

	==YwlE arginine phosphatase from Geobacillus stearothermophilus==		==YwlE arginine phosphatase from Geobacillus stearothermophilus==
-	<StructureSection load='4pic' size='340' side='right' caption='[[4pic]], [[Resolution\|resolution]] 1.40Å' scene=''>	+	<StructureSection load='4pic' size='340' side='right'caption='[[4pic]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4pic]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_12980 Atcc 12980~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PIC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4PIC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4pic]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PIC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~PO4~~:~~PHOSPHATE+ION~~'>~~PO4~~</scene>, <scene name='pdbligand=~~SO4~~:~~SULFATE~~+ION'>~~SO4~~</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=~~MSE~~:~~SELENOMETHIONINE~~'>~~MSE~~</scene>~~</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pic OCA], [https://pdbe.org/4pic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pic RCSB], [https://www.ebi.ac.uk/pdbsum/4pic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pic ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ywle ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 ATCC 12980])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4pic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pic OCA], [~~http~~://pdbe.org/4pic PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4pic RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4pic PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4pic ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PAP_GEOSE PAP_GEOSE] Catalyzes the specific dephosphorylation of phosphoarginine residues in proteins. Probably counteracts the protein arginine kinase McsB in vivo. Exhibits almost no activity against pTyr peptides. Protein arginine phosphorylation has a physiologically important role and is involved in the regulation of many critical cellular processes, such as protein homeostasis, motility, competence, and stringent and stress responses, by regulating gene expression and protein activity.<ref>PMID:23770242</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 12980~~]]	+	[[Category: Geobacillus stearothermophilus]]
-	[[Category: ~~Clausen, T~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Fuhrmann, J~~]]	+	[[Category: Clausen T]]
-	[[Category: ~~Arginine dephosphorylation]]~~	+	[[Category: Fuhrmann J]]
-	~~[[Category: Arginine phosphorylation]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Lmw ptp]]~~	+
-	~~[[Category: Phosphatase dimer]]~~	+
-	~~[[Category: Protein modification~~]]	+

OCA at 08:41, 22 November 2017

OCA — Wed, 22 Nov 2017 08:41:04 GMT

←Older revision		Revision as of 08:41, 22 November 2017
Line 3:		Line 3:
	<StructureSection load='4pic' size='340' side='right' caption='[[4pic]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='4pic' size='340' side='right' caption='[[4pic]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4pic]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PIC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4pic]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_12980 Atcc 12980]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PIC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ywle ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 ATCC 12980])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pic OCA], [http://pdbe.org/4pic PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pic RCSB], [http://www.ebi.ac.uk/pdbsum/4pic PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pic ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pic OCA], [http://pdbe.org/4pic PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pic RCSB], [http://www.ebi.ac.uk/pdbsum/4pic PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pic ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 12980]]
	[[Category: Clausen, T]]		[[Category: Clausen, T]]
	[[Category: Fuhrmann, J]]		[[Category: Fuhrmann, J]]

OCA at 12:49, 11 August 2016

OCA — Thu, 11 Aug 2016 12:49:38 GMT

←Older revision		Revision as of 12:49, 11 August 2016
Line 1:		Line 1:
		+
	==YwlE arginine phosphatase from Geobacillus stearothermophilus==		==YwlE arginine phosphatase from Geobacillus stearothermophilus==
	<StructureSection load='4pic' size='340' side='right' caption='[[4pic]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='4pic' size='340' side='right' caption='[[4pic]], [[Resolution\|resolution]] 1.40Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pic OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pic RCSB], [http://www.ebi.ac.uk/pdbsum/4pic PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pic OCA], [http://pdbe.org/4pic PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pic RCSB], [http://www.ebi.ac.uk/pdbsum/4pic PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pic ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4pic" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:51, 5 January 2015

OCA — Mon, 05 Jan 2015 08:51:26 GMT

←Older revision		Revision as of 08:51, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4pic]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PIC FirstGlance]. <br>		<table><tr><td colspan='2'>[[4pic]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PIC FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pic OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pic RCSB], [http://www.ebi.ac.uk/pdbsum/4pic PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pic OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pic RCSB], [http://www.ebi.ac.uk/pdbsum/4pic PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Clausen, T.]]	+	[[Category: Clausen, T]]
-	[[Category: Fuhrmann, J.]]	+	[[Category: Fuhrmann, J]]
	[[Category: Arginine dephosphorylation]]		[[Category: Arginine dephosphorylation]]
	[[Category: Arginine phosphorylation]]		[[Category: Arginine phosphorylation]]

OCA at 06:18, 11 June 2014

OCA — Wed, 11 Jun 2014 06:18:34 GMT

←Older revision		Revision as of 06:18, 11 June 2014
Line 13:		Line 13:
	Chasing phosphoarginine proteins: development of a selective enrichment method using a phosphatase trap.,Trentini DB, Fuhrmann J, Mechtler K, Clausen T Mol Cell Proteomics. 2014 May 13. pii: mcp.O113.035790. PMID:24825175<ref>PMID:24825175</ref>		Chasing phosphoarginine proteins: development of a selective enrichment method using a phosphatase trap.,Trentini DB, Fuhrmann J, Mechtler K, Clausen T Mol Cell Proteomics. 2014 May 13. pii: mcp.O113.035790. PMID:24825175<ref>PMID:24825175</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==

OCA at 09:33, 28 May 2014

OCA — Wed, 28 May 2014 09:33:25 GMT

←Older revision		Revision as of 09:33, 28 May 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==YwlE arginine phosphatase from Geobacillus stearothermophilus==
		+	<StructureSection load='4pic' size='340' side='right' caption='[[4pic]], [[Resolution\|resolution]] 1.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4pic]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PIC FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pic OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pic RCSB], [http://www.ebi.ac.uk/pdbsum/4pic PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Arginine phosphorylation is an emerging post-translational protein modification implicated in the bacterial stress response. Although early reports suggested that arginine phosphorylation also occurs in higher eukaryotes, its overall prevalence was never studied by modern mass spectrometry methods, owing to technical difficulties arising from the acid lability of phosphoarginine. As shown recently, the McsB and YwlE proteins from Bacillus subtilis function as a highly specific protein arginine kinase and phosphatase couple, shaping the phosphoarginine proteome. Using a B. subtilis DeltaywlE strain as source for arginine-phosphorylated proteins, we could adapt mass spectrometry (MS) protocols to the special chemical properties of the arginine modification. Despite this progress, the analysis of protein arginine phosphorylation in eukaryotes is still challenging, given the great abundance of serine/threonine phosphorylations that would compete with phosphoarginine during the phosphopeptide enrichment procedure as well as during data-dependent MS acquisition. We thus set out to establish a method to selectively enrich arginine-phosphorylated proteins as an initial step in the phosphoproteomic analysis. For this purpose, we developed a substrate-trapping mutant of the YwlE phosphatase that retains binding affinity towards arginine-phosphorylated proteins but cannot hydrolyze the captured substrates. By testing a number of active site substitutions, we identified a YwlE mutant (C9A) that stably binds to arginine-phosphorylated proteins. We further improved the substrate-trapping efficiency by impeding the oligomerization of the phosphatase mutant. The engineered YwlE trap efficiently captured arginine-phosphorylated proteins from complex B. subtilis DeltaywlE cell extracts, thus facilitating identification of phosphoarginine sites in the large pool of cellular protein modifications. In conclusion, we present a novel tool for the selective enrichment and subsequent MS analysis of arginine phosphorylation, which is a largely overlooked protein modification that may have an important impact for eukaryotic cell signaling.

-	~~The entry 4pic is ON HOLD~~	+	Chasing phosphoarginine proteins: development of a selective enrichment method using a phosphatase trap.,Trentini DB, Fuhrmann J, Mechtler K, Clausen T Mol Cell Proteomics. 2014 May 13. pii: mcp.O113.035790. PMID:24825175<ref>PMID:24825175</ref>

-	~~Authors~~: Clausen, T., Fuhrmann, J.	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~YwlE arginine phosphatase from Geobacillus stearothermophilus~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Clausen, T.]]
		+	[[Category: Fuhrmann, J.]]
		+	[[Category: Arginine dephosphorylation]]
		+	[[Category: Arginine phosphorylation]]
		+	[[Category: Hydrolase]]
		+	[[Category: Lmw ptp]]
		+	[[Category: Phosphatase dimer]]
		+	[[Category: Protein modification]]

OCA at 08:04, 21 May 2014

OCA — Wed, 21 May 2014 08:04:37 GMT

←Older revision		Revision as of 08:04, 21 May 2014
Line 5:		Line 5:
	Authors: Clausen, T., Fuhrmann, J.		Authors: Clausen, T., Fuhrmann, J.

-	Description: YwlE arginine phosphatase from ~~Geobacillu~~ stearothermophilus	+	Description: YwlE arginine phosphatase from Geobacillus stearothermophilus

OCA: Protected "4pic" [edit=sysop:move=sysop]

OCA — Wed, 14 May 2014 07:08:48 GMT

Protected "4pic" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:08, 14 May 2014

OCA: New page: '''Unreleased structure''' The entry 4pic is ON HOLD Authors: Clausen, T., Fuhrmann, J. Description: YwlE arginine phosphatase from Geobacillu stearothermophilus

OCA — Wed, 14 May 2014 07:08:48 GMT

New page: '''Unreleased structure''' The entry 4pic is ON HOLD Authors: Clausen, T., Fuhrmann, J. Description: YwlE arginine phosphatase from Geobacillu stearothermophilus

New page

'''Unreleased structure'''

The entry 4pic is ON HOLD

Authors: Clausen, T., Fuhrmann, J.

Description: YwlE arginine phosphatase from Geobacillu stearothermophilus