4pkf - Revision history

OCA at 00:44, 28 December 2023

OCA — Thu, 28 Dec 2023 00:44:59 GMT

←Older revision		Revision as of 00:44, 28 December 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4pkf]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thauera_aromatica Thauera aromatica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PKF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PKF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4pkf]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thauera_aromatica Thauera aromatica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PKF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PKF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.002Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pkf OCA], [https://pdbe.org/4pkf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pkf RCSB], [https://www.ebi.ac.uk/pdbsum/4pkf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pkf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pkf OCA], [https://pdbe.org/4pkf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pkf RCSB], [https://www.ebi.ac.uk/pdbsum/4pkf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pkf ProSAT]</span></td></tr>
	</table>		</table>

OCA at 13:10, 1 February 2023

OCA — Wed, 01 Feb 2023 13:10:01 GMT

←Older revision		Revision as of 13:10, 1 February 2023
Line 3:		Line 3:
	<StructureSection load='4pkf' size='340' side='right'caption='[[4pkf]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4pkf' size='340' side='right'caption='[[4pkf]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4pkf]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Dsm_6984 Dsm 6984~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PKF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4PKF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4pkf]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thauera_aromatica Thauera aromatica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PKF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PKF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pkc\|4pkc]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pkf OCA], [https://pdbe.org/4pkf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pkf RCSB], [https://www.ebi.ac.uk/pdbsum/4pkf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pkf ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tutD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=59405 DSM 6984]), tutG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=59405 DSM 6984]), tutF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=59405 DSM 6984])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Benzylsuccinate_synthase Benzylsuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.11 4.1.99.11] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4pkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pkf OCA], [~~http~~://pdbe.org/4pkf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4pkf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4pkf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4pkf ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O68395_THAAR O68395_THAAR]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Benzylsuccinate synthase]]
-	[[Category: Dsm 6984]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Drennan, C L~~]]	+	[[Category: Thauera aromatica]]
-	[[Category: ~~Funk, M A~~]]	+	[[Category: Drennan CL]]
-	[[Category: ~~Complex]]~~	+	[[Category: Funk MA]]
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Radical~~]]	+

OCA at 08:08, 17 April 2019

OCA — Wed, 17 Apr 2019 08:08:21 GMT

←Older revision		Revision as of 08:08, 17 April 2019
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	==Benzylsuccinate synthase alpha-beta-gamma complex==		==Benzylsuccinate synthase alpha-beta-gamma complex==
-	<StructureSection load='4pkf' size='340' side='right' caption='[[4pkf]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='4pkf' size='340' side='right'caption='[[4pkf]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4pkf]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PKF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PKF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4pkf]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_6984 Dsm 6984]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PKF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PKF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pkc\|4pkc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pkc\|4pkc]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tutD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=59405 DSM 6984]), tutG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=59405 DSM 6984]), tutF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=59405 DSM 6984])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Benzylsuccinate_synthase Benzylsuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.11 4.1.99.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Benzylsuccinate_synthase Benzylsuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.11 4.1.99.11] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pkf OCA], [http://pdbe.org/4pkf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pkf RCSB], [http://www.ebi.ac.uk/pdbsum/4pkf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pkf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pkf OCA], [http://pdbe.org/4pkf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pkf RCSB], [http://www.ebi.ac.uk/pdbsum/4pkf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pkf ProSAT]</span></td></tr>
Line 23:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Benzylsuccinate synthase]]		[[Category: Benzylsuccinate synthase]]
		+	[[Category: Dsm 6984]]
		+	[[Category: Large Structures]]
	[[Category: Drennan, C L]]		[[Category: Drennan, C L]]
	[[Category: Funk, M A]]		[[Category: Funk, M A]]

OCA at 21:14, 11 August 2016

OCA — Thu, 11 Aug 2016 21:14:49 GMT

←Older revision		Revision as of 21:14, 11 August 2016
Line 1:		Line 1:
		+
	==Benzylsuccinate synthase alpha-beta-gamma complex==		==Benzylsuccinate synthase alpha-beta-gamma complex==
	<StructureSection load='4pkf' size='340' side='right' caption='[[4pkf]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4pkf' size='340' side='right' caption='[[4pkf]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pkc\|4pkc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pkc\|4pkc]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Benzylsuccinate_synthase Benzylsuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.11 4.1.99.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Benzylsuccinate_synthase Benzylsuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.11 4.1.99.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pkf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pkf RCSB], [http://www.ebi.ac.uk/pdbsum/4pkf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pkf OCA], [http://pdbe.org/4pkf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pkf RCSB], [http://www.ebi.ac.uk/pdbsum/4pkf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pkf ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4pkf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:44, 20 January 2015

OCA — Tue, 20 Jan 2015 11:44:41 GMT

←Older revision		Revision as of 11:44, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4pkf]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PKF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PKF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4pkf]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PKF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PKF FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pkc\|4pkc]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pkc\|4pkc]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Benzylsuccinate_synthase Benzylsuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.11 4.1.99.11] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Benzylsuccinate_synthase Benzylsuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.11 4.1.99.11] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pkf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pkf RCSB], [http://www.ebi.ac.uk/pdbsum/4pkf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pkf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pkf RCSB], [http://www.ebi.ac.uk/pdbsum/4pkf PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Benzylsuccinate synthase]]		[[Category: Benzylsuccinate synthase]]
-	[[Category: Drennan, C L.]]	+	[[Category: Drennan, C L]]
-	[[Category: Funk, M A.]]	+	[[Category: Funk, M A]]
	[[Category: Complex]]		[[Category: Complex]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Radical]]		[[Category: Radical]]

OCA at 09:07, 16 July 2014

OCA — Wed, 16 Jul 2014 09:07:56 GMT

←Older revision		Revision as of 09:07, 16 July 2014
Line 8:		Line 8:
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pkf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pkf RCSB], [http://www.ebi.ac.uk/pdbsum/4pkf PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pkf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pkf RCSB], [http://www.ebi.ac.uk/pdbsum/4pkf PDBsum]</span></td></tr>
	<table>		<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Anaerobic degradation of the environmental pollutant toluene is initiated by the glycyl radical enzyme benzylsuccinate synthase (BSS), which catalyzes the radical addition of toluene to fumarate, forming benzylsuccinate. We have determined crystal structures of the catalytic alpha-subunit of BSS with its accessory subunits beta and gamma, which both bind a [4Fe-4S] cluster and are essential for BSS activity in vivo. We find that BSSalpha has the common glycyl radical enzyme fold, a 10-stranded beta/alpha-barrel that surrounds the glycyl radical cofactor and active site. Both accessory subunits beta and gamma display folds related to high potential iron-sulfur proteins but differ substantially from each other in how they interact with the alpha-subunit. BSSgamma binds distally to the active site, burying a hydrophobic region of BSSalpha, whereas BSSbeta binds to a hydrophilic surface of BSSalpha that is proximal to the active site. To further investigate the function of BSSbeta, we determined the structure of a BSSalphagamma complex. Remarkably, we find that the barrel partially opens, allowing the C-terminal region of BSSalpha that houses the glycyl radical to shift within the barrel toward an exit pathway. The structural changes that we observe in the BSSalphagamma complex center around the crucial glycyl radical domain, thus suggesting a role for BSSbeta in modulating the conformational dynamics required for enzyme activity. Accompanying proteolysis experiments support these structural observations.
		+
		+	Structures of benzylsuccinate synthase elucidate roles of accessory subunits in glycyl radical enzyme activation and activity.,Funk MA, Judd ET, Marsh EN, Elliott SJ, Drennan CL Proc Natl Acad Sci U S A. 2014 Jun 30. pii: 201405983. PMID:24982148<ref>PMID:24982148</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:51, 2 July 2014

OCA — Wed, 02 Jul 2014 08:51:13 GMT

←Older revision		Revision as of 08:51, 2 July 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Benzylsuccinate synthase alpha-beta-gamma complex==
-		+	<StructureSection load='4pkf' size='340' side='right' caption='[[4pkf]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~The entry~~ 4pkf is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4pkf]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PKF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PKF FirstGlance]. <br>
-	~~Authors~~: ~~Funk~~, M.A., ~~Drennan~~, C.L.	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene><br>
-		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pkc\|4pkc]]</td></tr>
-	~~Description~~: Benzylsuccinate synthase ~~alpha-beta-gamma complex~~	+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Benzylsuccinate_synthase Benzylsuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.11 4.1.99.11] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pkf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pkf RCSB], [http://www.ebi.ac.uk/pdbsum/4pkf PDBsum]</span></td></tr>
		+	<table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Benzylsuccinate synthase]]
		+	[[Category: Drennan, C L.]]
		+	[[Category: Funk, M A.]]
		+	[[Category: Complex]]
		+	[[Category: Lyase]]
		+	[[Category: Radical]]

OCA at 04:55, 4 June 2014

OCA — Wed, 04 Jun 2014 04:55:36 GMT

←Older revision		Revision as of 04:55, 4 June 2014
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4pkf is ON HOLD	+	The entry 4pkf is ON HOLD until Paper Publication

	Authors: Funk, M.A., Drennan, C.L.		Authors: Funk, M.A., Drennan, C.L.

	Description: Benzylsuccinate synthase alpha-beta-gamma complex		Description: Benzylsuccinate synthase alpha-beta-gamma complex

OCA: Protected "4pkf" [edit=sysop:move=sysop]

OCA — Wed, 21 May 2014 08:07:42 GMT

Protected "4pkf" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:07, 21 May 2014

OCA: New page: '''Unreleased structure''' The entry 4pkf is ON HOLD Authors: Funk, M.A., Drennan, C.L. Description: Benzylsuccinate synthase alpha-beta-gamma complex

OCA — Wed, 21 May 2014 08:07:40 GMT

New page: '''Unreleased structure''' The entry 4pkf is ON HOLD Authors: Funk, M.A., Drennan, C.L. Description: Benzylsuccinate synthase alpha-beta-gamma complex

New page

'''Unreleased structure'''

The entry 4pkf is ON HOLD

Authors: Funk, M.A., Drennan, C.L.

Description: Benzylsuccinate synthase alpha-beta-gamma complex