4pks - Revision history

OCA at 00:45, 28 December 2023

OCA — Thu, 28 Dec 2023 00:45:10 GMT

←Older revision		Revision as of 00:45, 28 December 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4pks]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PKS FirstGlance]. <br>		<table><tr><td colspan='2'>[[4pks]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PKS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=30H:N~2~-[(4-FLUORO-3-METHYLPHENYL)SULFONYL]-N-HYDROXY-N~2~-(PYRIDIN-3-YLMETHYL)-D-ALANINAMIDE'>30H</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=30H:N~2~-[(4-FLUORO-3-METHYLPHENYL)SULFONYL]-N-HYDROXY-N~2~-(PYRIDIN-3-YLMETHYL)-D-ALANINAMIDE'>30H</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pks OCA], [https://pdbe.org/4pks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pks RCSB], [https://www.ebi.ac.uk/pdbsum/4pks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pks ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pks OCA], [https://pdbe.org/4pks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pks RCSB], [https://www.ebi.ac.uk/pdbsum/4pks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pks ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:16, 8 February 2023

OCA — Wed, 08 Feb 2023 07:16:29 GMT

←Older revision		Revision as of 07:16, 8 February 2023
Line 3:		Line 3:
	<StructureSection load='4pks' size='340' side='right'caption='[[4pks]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4pks' size='340' side='right'caption='[[4pks]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4pks]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var.~~ anthracis~~" (cohn 1872) smith et al. 1946~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PKS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4PKS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4pks]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PKS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=30H:N~2~-[(4-FLUORO-3-METHYLPHENYL)SULFONYL]-N-HYDROXY-N~2~-(PYRIDIN-3-YLMETHYL)-D-ALANINAMIDE'>30H</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=30H:N~2~-[(4-FLUORO-3-METHYLPHENYL)SULFONYL]-N-HYDROXY-N~2~-(PYRIDIN-3-YLMETHYL)-D-ALANINAMIDE'>30H</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jky\|1jky]], [[1j7n\|1j7n]], [[1pwp\|1pwp]], [[1pwq\|1pwq]], [[1pwu\|1pwu]], [[1pwv\|1pwv]], [[1pww\|1pww]], [[1zxv\|1zxv]], [[1yqy\|1yqy]], [[4dv8\|4dv8]], [[4pkq\|4pkq]], [[4pkr\|4pkr]], [[4pkt\|4pkt]], [[4pku\|4pku]], [[4pkv\|4pkv]], [[4pkw\|4pkw]]</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pks OCA], [https://pdbe.org/4pks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pks RCSB], [https://www.ebi.ac.uk/pdbsum/4pks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pks ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lef, pXO1-107, BXA0172, GBAA_pXO1_0172 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.83 3.4.24.83] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4pks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pks OCA], [~~http~~://pdbe.org/4pks PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4pks RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4pks PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4pks ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LEF_BACAN LEF_BACAN]] One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. LF is the lethal factor that, when associated with PA, causes death. LF is not toxic by itself. It is a protease that cleaves the N-terminal of most dual specificity mitogen-activated protein kinase kinases (MAPKKs or MAP2Ks) (except for MAP2K5). Cleavage invariably occurs within the N-terminal proline-rich region preceding the kinase domain, thus disrupting a sequence involved in directing specific protein-protein interactions necessary for the assembly of signaling complexes. There may be other cytosolic targets of LF involved in cytotoxicity. The proteasome may mediate a toxic process initiated by LF in the cell cytosol involving degradation of unidentified molecules that are essential for macrophage homeostasis. This is an early step in LeTx intoxication, but it is downstream of the cleavage by LF of MEK1 or other putative substrates.<ref>PMID:9563949</ref> <ref>PMID:9703991</ref> <ref>PMID:10475971</ref> <ref>PMID:11104681</ref> <ref>PMID:10338520</ref>	+	[https://www.uniprot.org/uniprot/LEF_BACAN LEF_BACAN] One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. LF is the lethal factor that, when associated with PA, causes death. LF is not toxic by itself. It is a protease that cleaves the N-terminal of most dual specificity mitogen-activated protein kinase kinases (MAPKKs or MAP2Ks) (except for MAP2K5). Cleavage invariably occurs within the N-terminal proline-rich region preceding the kinase domain, thus disrupting a sequence involved in directing specific protein-protein interactions necessary for the assembly of signaling complexes. There may be other cytosolic targets of LF involved in cytotoxicity. The proteasome may mediate a toxic process initiated by LF in the cell cytosol involving degradation of unidentified molecules that are essential for macrophage homeostasis. This is an early step in LeTx intoxication, but it is downstream of the cleavage by LF of MEK1 or other putative substrates.<ref>PMID:9563949</ref> <ref>PMID:9703991</ref> <ref>PMID:10475971</ref> <ref>PMID:11104681</ref> <ref>PMID:10338520</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Hydrolase~~]]	+	[[Category: Bacillus anthracis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Finzel, B C]]~~	+	[[Category: De la Mora T]]
-	~~[[Category: Maize, K M]]~~	+	[[Category: Finzel BC]]
-	~~[[Category:~~ Mora, T ~~De la~~]]	+	[[Category: Maize KM]]
-	[[Category: ~~Anthrax toxin~~]]	+
-	[[Category: ~~Hydrolase-hydrolase inhibitor complex]]~~	+
-	~~[[Category: Lethal factor]]~~	+
-	~~[[Category: Ligand-induced conformational change]]~~	+
-	~~[[Category: Metalloprotease]]~~	+
-	~~[[Category: Metalloproteinase]]~~	+
-	~~[[Category: Structural dynamic~~]]	+

OCA at 15:37, 11 December 2019

OCA — Wed, 11 Dec 2019 15:37:17 GMT

←Older revision		Revision as of 15:37, 11 December 2019
Line 1:		Line 1:

	==Anthrax toxin lethal factor with bound small molecule inhibitor 11==		==Anthrax toxin lethal factor with bound small molecule inhibitor 11==
-	<StructureSection load='4pks' size='340' side='right' caption='[[4pks]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='4pks' size='340' side='right'caption='[[4pks]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4pks]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var. anthracis" (cohn 1872) smith et al. 1946]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PKS FirstGlance]. <br>		<table><tr><td colspan='2'>[[4pks]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var. anthracis" (cohn 1872) smith et al. 1946]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PKS FirstGlance]. <br>
Line 21:		Line 21:
	</div>		</div>
	<div class="pdbe-citations 4pks" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4pks" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Anthrax lethal factor 3D structures\|Anthrax lethal factor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
		+	[[Category: Large Structures]]
	[[Category: Finzel, B C]]		[[Category: Finzel, B C]]
	[[Category: Maize, K M]]		[[Category: Maize, K M]]

OCA at 12:01, 6 November 2017

OCA — Mon, 06 Nov 2017 12:01:24 GMT

←Older revision		Revision as of 12:01, 6 November 2017
Line 3:		Line 3:
	<StructureSection load='4pks' size='340' side='right' caption='[[4pks]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4pks' size='340' side='right' caption='[[4pks]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4pks]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PKS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4pks]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var. anthracis" (cohn 1872) smith et al. 1946]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PKS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=30H:N~2~-[(4-FLUORO-3-METHYLPHENYL)SULFONYL]-N-HYDROXY-N~2~-(PYRIDIN-3-YLMETHYL)-D-ALANINAMIDE'>30H</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=30H:N~2~-[(4-FLUORO-3-METHYLPHENYL)SULFONYL]-N-HYDROXY-N~2~-(PYRIDIN-3-YLMETHYL)-D-ALANINAMIDE'>30H</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jky\|1jky]], [[1j7n\|1j7n]], [[1pwp\|1pwp]], [[1pwq\|1pwq]], [[1pwu\|1pwu]], [[1pwv\|1pwv]], [[1pww\|1pww]], [[1zxv\|1zxv]], [[1yqy\|1yqy]], [[4dv8\|4dv8]], [[4pkq\|4pkq]], [[4pkr\|4pkr]], [[4pkt\|4pkt]], [[4pku\|4pku]], [[4pkv\|4pkv]], [[4pkw\|4pkw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jky\|1jky]], [[1j7n\|1j7n]], [[1pwp\|1pwp]], [[1pwq\|1pwq]], [[1pwu\|1pwu]], [[1pwv\|1pwv]], [[1pww\|1pww]], [[1zxv\|1zxv]], [[1yqy\|1yqy]], [[4dv8\|4dv8]], [[4pkq\|4pkq]], [[4pkr\|4pkr]], [[4pkt\|4pkt]], [[4pku\|4pku]], [[4pkv\|4pkv]], [[4pkw\|4pkw]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lef, pXO1-107, BXA0172, GBAA_pXO1_0172 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.83 3.4.24.83] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.83 3.4.24.83] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pks OCA], [http://pdbe.org/4pks PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pks RCSB], [http://www.ebi.ac.uk/pdbsum/4pks PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pks ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pks OCA], [http://pdbe.org/4pks PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pks RCSB], [http://www.ebi.ac.uk/pdbsum/4pks PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pks ProSAT]</span></td></tr>
Line 20:		Line 21:
	</div>		</div>
	<div class="pdbe-citations 4pks" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4pks" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Anthrax Lethal Factor\|Anthrax Lethal Factor]]
	== References ==		== References ==
	<references/>		<references/>
Line 32:		Line 30:
	[[Category: Mora, T De la]]		[[Category: Mora, T De la]]
	[[Category: Anthrax toxin]]		[[Category: Anthrax toxin]]
		+	[[Category: Hydrolase-hydrolase inhibitor complex]]
	[[Category: Lethal factor]]		[[Category: Lethal factor]]
	[[Category: Ligand-induced conformational change]]		[[Category: Ligand-induced conformational change]]

OCA at 17:06, 4 January 2017

OCA — Wed, 04 Jan 2017 17:06:54 GMT

←Older revision		Revision as of 17:06, 4 January 2017
Line 1:		Line 1:
		+
	==Anthrax toxin lethal factor with bound small molecule inhibitor 11==		==Anthrax toxin lethal factor with bound small molecule inhibitor 11==
	<StructureSection load='4pks' size='340' side='right' caption='[[4pks]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4pks' size='340' side='right' caption='[[4pks]], [[Resolution\|resolution]] 2.30Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jky\|1jky]], [[1j7n\|1j7n]], [[1pwp\|1pwp]], [[1pwq\|1pwq]], [[1pwu\|1pwu]], [[1pwv\|1pwv]], [[1pww\|1pww]], [[1zxv\|1zxv]], [[1yqy\|1yqy]], [[4dv8\|4dv8]], [[4pkq\|4pkq]], [[4pkr\|4pkr]], [[4pkt\|4pkt]], [[4pku\|4pku]], [[4pkv\|4pkv]], [[4pkw\|4pkw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jky\|1jky]], [[1j7n\|1j7n]], [[1pwp\|1pwp]], [[1pwq\|1pwq]], [[1pwu\|1pwu]], [[1pwv\|1pwv]], [[1pww\|1pww]], [[1zxv\|1zxv]], [[1yqy\|1yqy]], [[4dv8\|4dv8]], [[4pkq\|4pkq]], [[4pkr\|4pkr]], [[4pkt\|4pkt]], [[4pku\|4pku]], [[4pkv\|4pkv]], [[4pkw\|4pkw]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.83 3.4.24.83] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.83 3.4.24.83] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pks OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pks RCSB], [http://www.ebi.ac.uk/pdbsum/4pks PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pks OCA], [http://pdbe.org/4pks PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pks RCSB], [http://www.ebi.ac.uk/pdbsum/4pks PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pks ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4pks" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 09:57, 7 February 2015

OCA — Sat, 07 Feb 2015 09:57:06 GMT

←Older revision		Revision as of 09:57, 7 February 2015
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[Anthrax Lethal Factor\|Anthrax Lethal Factor]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:59, 25 December 2014

OCA — Thu, 25 Dec 2014 10:59:08 GMT

←Older revision		Revision as of 10:59, 25 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pks OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pks RCSB], [http://www.ebi.ac.uk/pdbsum/4pks PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pks OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pks RCSB], [http://www.ebi.ac.uk/pdbsum/4pks PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LEF_BACAN LEF_BACAN]] One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. LF is the lethal factor that, when associated with PA, causes death. LF is not toxic by itself. It is a protease that cleaves the N-terminal of most dual specificity mitogen-activated protein kinase kinases (MAPKKs or MAP2Ks) (except for MAP2K5). Cleavage invariably occurs within the N-terminal proline-rich region preceding the kinase domain, thus disrupting a sequence involved in directing specific protein-protein interactions necessary for the assembly of signaling complexes. There may be other cytosolic targets of LF involved in cytotoxicity. The proteasome may mediate a toxic process initiated by LF in the cell cytosol involving degradation of unidentified molecules that are essential for macrophage homeostasis. This is an early step in LeTx intoxication, but it is downstream of the cleavage by LF of MEK1 or other putative substrates.<ref>PMID:9563949</ref> <ref>PMID:9703991</ref> <ref>PMID:10475971</ref> <ref>PMID:11104681</ref> <ref>PMID:10338520</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 06:11, 26 November 2014

OCA — Wed, 26 Nov 2014 06:11:05 GMT

←Older revision		Revision as of 06:11, 26 November 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pks OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pks RCSB], [http://www.ebi.ac.uk/pdbsum/4pks PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pks OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pks RCSB], [http://www.ebi.ac.uk/pdbsum/4pks PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The secreted anthrax toxin consists of three components: the protective antigen (PA), edema factor (EF) and lethal factor (LF). LF, a zinc metalloproteinase, compromises the host immune system primarily by targeting mitogen-activated protein kinase kinases in macrophages. Peptide substrates and small-molecule inhibitors bind LF in the space between domains 3 and 4 of the hydrolase. Domain 3 is attached on a hinge to domain 2 via residues Ile300 and Pro385, and can move through an angular arc of greater than 35 degrees in response to the binding of different ligands. Here, multiple LF structures including five new complexes with co-crystallized inhibitors are compared and three frequently populated LF conformational states termed `bioactive', `open' and `tight' are identified. The bioactive position is observed with large substrate peptides and leaves all peptide-recognition subsites open and accessible. The tight state is seen in unliganded and small-molecule complex structures. In this state, domain 3 is clamped over certain substrate subsites, blocking access. The open position appears to be an intermediate state between these extremes and is observed owing to steric constraints imposed by specific bound ligands. The tight conformation may be the lowest-energy conformation among the reported structures, as it is the position observed with no bound ligand, while the open and bioactive conformations are likely to be ligand-induced.
		+
		+	Anthrax toxin lethal factor domain 3 is highly mobile and responsive to ligand binding.,Maize KM, Kurbanov EK, De La Mora-Rey T, Geders TW, Hwang DJ, Walters MA, Johnson RL, Amin EA, Finzel BC Acta Crystallogr D Biol Crystallogr. 2014 Nov;70(Pt 11):2813-22. doi:, 10.1107/S1399004714018161. Epub 2014 Oct 16. PMID:25372673<ref>PMID:25372673</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:54, 12 November 2014

OCA — Wed, 12 Nov 2014 06:54:07 GMT

←Older revision		Revision as of 06:54, 12 November 2014
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-	'''~~Unreleased structure~~'''	+	==Anthrax toxin lethal factor with bound small molecule inhibitor 11==
-		+	<StructureSection load='4pks' size='340' side='right' caption='[[4pks]], [[Resolution\|resolution]] 2.30Å' scene=''>
-	~~The entry~~ 4pks is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4pks]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PKS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PKS FirstGlance]. <br>
-	~~Authors~~: ~~Maize~~, K.M., ~~De la Mora~~, T., Finzel, B.C.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=30H:N~2~-[(4-FLUORO-3-METHYLPHENYL)SULFONYL]-N-HYDROXY-N~2~-(PYRIDIN-3-YLMETHYL)-D-ALANINAMIDE'>30H</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jky\|1jky]], [[1j7n\|1j7n]], [[1pwp\|1pwp]], [[1pwq\|1pwq]], [[1pwu\|1pwu]], [[1pwv\|1pwv]], [[1pww\|1pww]], [[1zxv\|1zxv]], [[1yqy\|1yqy]], [[4dv8\|4dv8]], [[4pkq\|4pkq]], [[4pkr\|4pkr]], [[4pkt\|4pkt]], [[4pku\|4pku]], [[4pkv\|4pkv]], [[4pkw\|4pkw]]</td></tr>
-	~~Description~~: Anthrax toxin ~~lethal~~ factor ~~with bound small molecule inhibitor 11~~	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.83 3.4.24.83] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pks OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pks RCSB], [http://www.ebi.ac.uk/pdbsum/4pks PDBsum]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Hydrolase]]
		+	[[Category: Finzel, B C]]
		+	[[Category: Maize, K M]]
		+	[[Category: Mora, T De la]]
		+	[[Category: Anthrax toxin]]
		+	[[Category: Lethal factor]]
		+	[[Category: Ligand-induced conformational change]]
		+	[[Category: Metalloprotease]]
		+	[[Category: Metalloproteinase]]
		+	[[Category: Structural dynamic]]

OCA at 07:54, 11 June 2014

OCA — Wed, 11 Jun 2014 07:54:46 GMT

←Older revision		Revision as of 07:54, 11 June 2014
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4pks is ON HOLD	+	The entry 4pks is ON HOLD until Paper Publication

	Authors: Maize, K.M., De la Mora, T., Finzel, B.C.		Authors: Maize, K.M., De la Mora, T., Finzel, B.C.

	Description: Anthrax toxin lethal factor with bound small molecule inhibitor 11		Description: Anthrax toxin lethal factor with bound small molecule inhibitor 11