4pls - Revision history

OCA at 12:23, 20 December 2023

OCA — Wed, 20 Dec 2023 12:23:44 GMT

←Older revision		Revision as of 12:23, 20 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4pls]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PLS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PLS FirstGlance]. <br>		<table><tr><td colspan='2'>[[4pls]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PLS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PLS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.35Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pls OCA], [https://pdbe.org/4pls PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pls RCSB], [https://www.ebi.ac.uk/pdbsum/4pls PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pls ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pls OCA], [https://pdbe.org/4pls PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pls RCSB], [https://www.ebi.ac.uk/pdbsum/4pls PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pls ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:17, 8 February 2023

OCA — Wed, 08 Feb 2023 07:17:46 GMT

←Older revision		Revision as of 07:17, 8 February 2023
Line 3:		Line 3:
	<StructureSection load='4pls' size='340' side='right'caption='[[4pls]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='4pls' size='340' side='right'caption='[[4pls]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4pls]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Synthetic_construct_sequences~~ Synthetic construct ~~sequences~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PLS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4PLS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4pls]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PLS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PLS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4pls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pls OCA], [~~http~~://pdbe.org/4pls PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4pls RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4pls PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4pls ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pls OCA], [https://pdbe.org/4pls PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pls RCSB], [https://www.ebi.ac.uk/pdbsum/4pls PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pls ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Synthetic construct ~~sequences~~]]	+	[[Category: Synthetic construct]]
-	[[Category: Madhurantakam, C]]	+	[[Category: Madhurantakam C]]
-	[[Category: Mittl~~, P R~~]]	+	[[Category: Mittl PR]]
-	[[Category: Plueckthun, A]]	+	[[Category: Plueckthun A]]
-	[[Category: Reichen, C]]	+	[[Category: Reichen C]]
-	~~[[Category: Designed armadillo repeat protein]]~~	+
-	~~[[Category: Peptide binding protein]]~~	+
-	~~[[Category: Protein engineering~~]]	+

OCA at 08:01, 17 April 2019

OCA — Wed, 17 Apr 2019 08:01:58 GMT

←Older revision		Revision as of 08:01, 17 April 2019
Line 1:		Line 1:

	==Crystal Structures of Designed Armadillo Repeat Proteins: Implications of Construct Design and Crystallization Conditions on Overall Structure.==		==Crystal Structures of Designed Armadillo Repeat Proteins: Implications of Construct Design and Crystallization Conditions on Overall Structure.==
-	<StructureSection load='4pls' size='340' side='right' caption='[[4pls]], [[Resolution\|resolution]] 2.35Å' scene=''>	+	<StructureSection load='4pls' size='340' side='right'caption='[[4pls]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4pls]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PLS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PLS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4pls]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PLS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PLS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pls OCA], [http://pdbe.org/4pls PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pls RCSB], [http://www.ebi.ac.uk/pdbsum/4pls PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pls ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pls OCA], [http://pdbe.org/4pls PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pls RCSB], [http://www.ebi.ac.uk/pdbsum/4pls PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pls ProSAT]</span></td></tr>
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Synthetic construct sequences]]
	[[Category: Madhurantakam, C]]		[[Category: Madhurantakam, C]]
	[[Category: Mittl, P R]]		[[Category: Mittl, P R]]

OCA at 17:07, 4 January 2017

OCA — Wed, 04 Jan 2017 17:07:46 GMT

←Older revision		Revision as of 17:07, 4 January 2017
Line 1:		Line 1:
		+
	==Crystal Structures of Designed Armadillo Repeat Proteins: Implications of Construct Design and Crystallization Conditions on Overall Structure.==		==Crystal Structures of Designed Armadillo Repeat Proteins: Implications of Construct Design and Crystallization Conditions on Overall Structure.==
	<StructureSection load='4pls' size='340' side='right' caption='[[4pls]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='4pls' size='340' side='right' caption='[[4pls]], [[Resolution\|resolution]] 2.35Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4pls]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PLS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PLS FirstGlance]. <br>		<table><tr><td colspan='2'>[[4pls]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PLS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PLS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pls OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pls RCSB], [http://www.ebi.ac.uk/pdbsum/4pls PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pls OCA], [http://pdbe.org/4pls PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pls RCSB], [http://www.ebi.ac.uk/pdbsum/4pls PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pls ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4pls" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:08, 10 December 2014

OCA — Wed, 10 Dec 2014 16:08:06 GMT

←Older revision		Revision as of 16:08, 10 December 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal Structures of Designed Armadillo Repeat Proteins: Implications of Construct Design and Crystallization Conditions on Overall Structure.==
		+	<StructureSection load='4pls' size='340' side='right' caption='[[4pls]], [[Resolution\|resolution]] 2.35Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4pls]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PLS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PLS FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pls FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pls OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pls RCSB], [http://www.ebi.ac.uk/pdbsum/4pls PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Designed armadillo repeat proteins (dArmRP) are promising modular proteins for the engineering of binding molecules that recognize extended polypeptide chains. We determined the structure of a dArmRP containing five internal repeats and 3rd generation capping repeats in three different states by X-ray crystallography: without N-terminal His6 -tag and in the presence of calcium (YM5 A/Ca2+ ), without N-terminal His6 -tag and in the absence of calcium (YM5 A), and with N-terminal His6 -tag and in the presence of calcium (His-YM5 A/Ca2+ ). All structures show different quaternary structures and superhelical parameters. His-YM5 A/Ca2+ forms a crystallographic dimer, which is bridged by the His6 -tag, YM5 A/Ca2+ forms a domain-swapped tetramer, and only in the absence of calcium and the His6 -tag, YM5 A forms a monomer. The changes of superhelical parameters are a consequence of calcium binding, because calcium ions interact with negatively charged residues, which can also participate in the modulation of helix dipole moments between adjacent repeats. These observations are important for further optimizations of dArmRPs and provide a general illustration of how construct design and crystallization conditions can influence the exact structure of the investigated protein.

-	~~The entry 4pls is ON HOLD~~	+	Crystal Structures of Designed Armadillo Repeat Proteins: Implications of Construct Design and Crystallization Conditions on Overall Structure.,Reichen C, Madhurantakam C, Pluckthun A, Mittl PR Protein Sci. 2014 Aug 13. doi: 10.1002/pro.2535. PMID:25132085<ref>PMID:25132085</ref>

-	~~Authors: Reichen~~, C.~~, Madhurantakam, C~~.~~, Plueckthun, A~~., Mittl, P.R.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal Structures of~~ Designed ~~Armadillo Repeat Proteins~~: ~~Implications of Construct Design and Crystallization Conditions on Overall Structure.~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Madhurantakam, C]]
		+	[[Category: Mittl, P R]]
		+	[[Category: Plueckthun, A]]
		+	[[Category: Reichen, C]]
		+	[[Category: Designed armadillo repeat protein]]
		+	[[Category: Peptide binding protein]]
		+	[[Category: Protein engineering]]

OCA at 11:16, 19 November 2014

OCA — Wed, 19 Nov 2014 11:16:37 GMT

←Older revision		Revision as of 11:16, 19 November 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4pls is ON HOLD ~~until sometime in the future~~	+	The entry 4pls is ON HOLD

	Authors: Reichen, C., Madhurantakam, C., Plueckthun, A., Mittl, P.R.		Authors: Reichen, C., Madhurantakam, C., Plueckthun, A., Mittl, P.R.

	Description: Crystal Structures of Designed Armadillo Repeat Proteins: Implications of Construct Design and Crystallization Conditions on Overall Structure.		Description: Crystal Structures of Designed Armadillo Repeat Proteins: Implications of Construct Design and Crystallization Conditions on Overall Structure.

OCA at 07:27, 13 August 2014

OCA — Wed, 13 Aug 2014 07:27:38 GMT

←Older revision		Revision as of 07:27, 13 August 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4pls is ON HOLD until ~~Paper Publication~~	+	The entry 4pls is ON HOLD until sometime in the future

	Authors: Reichen, C., Madhurantakam, C., Plueckthun, A., Mittl, P.R.		Authors: Reichen, C., Madhurantakam, C., Plueckthun, A., Mittl, P.R.

	Description: Crystal Structures of Designed Armadillo Repeat Proteins: Implications of Construct Design and Crystallization Conditions on Overall Structure.		Description: Crystal Structures of Designed Armadillo Repeat Proteins: Implications of Construct Design and Crystallization Conditions on Overall Structure.

OCA at 04:55, 4 June 2014

OCA — Wed, 04 Jun 2014 04:55:49 GMT

←Older revision		Revision as of 04:55, 4 June 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4pls is ON HOLD	+	The entry 4pls is ON HOLD until Paper Publication

	Authors: Reichen, C., Madhurantakam, C., Plueckthun, A., Mittl, P.R.		Authors: Reichen, C., Madhurantakam, C., Plueckthun, A., Mittl, P.R.

	Description: Crystal Structures of Designed Armadillo Repeat Proteins: Implications of Construct Design and Crystallization Conditions on Overall Structure.		Description: Crystal Structures of Designed Armadillo Repeat Proteins: Implications of Construct Design and Crystallization Conditions on Overall Structure.

OCA: Protected "4pls" [edit=sysop:move=sysop]

OCA — Wed, 28 May 2014 08:16:17 GMT

Protected "4pls" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:16, 28 May 2014

OCA: New page: '''Unreleased structure''' The entry 4pls is ON HOLD Authors: Reichen, C., Madhurantakam, C., Plueckthun, A., Mittl, P.R. Description: Crystal Structures of Designed Armadillo Repeat P...

OCA — Wed, 28 May 2014 08:16:16 GMT

New page: '''Unreleased structure''' The entry 4pls is ON HOLD Authors: Reichen, C., Madhurantakam, C., Plueckthun, A., Mittl, P.R. Description: Crystal Structures of Designed Armadillo Repeat P...

New page

'''Unreleased structure'''

The entry 4pls is ON HOLD

Authors: Reichen, C., Madhurantakam, C., Plueckthun, A., Mittl, P.R.

Description: Crystal Structures of Designed Armadillo Repeat Proteins: Implications of Construct Design and Crystallization Conditions on Overall Structure.