4pqg - Revision history

OCA at 12:42, 1 March 2024

OCA — Fri, 01 Mar 2024 12:42:35 GMT

←Older revision		Revision as of 12:42, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4pqg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PQG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PQG FirstGlance]. <br>		<table><tr><td colspan='2'>[[4pqg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PQG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PQG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pqg OCA], [https://pdbe.org/4pqg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pqg RCSB], [https://www.ebi.ac.uk/pdbsum/4pqg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pqg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pqg OCA], [https://pdbe.org/4pqg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pqg RCSB], [https://www.ebi.ac.uk/pdbsum/4pqg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pqg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/GTFA_STRPN GTFA_STRPN] Required for the polymorphic O-glycosylation of serine-rich repeat protein PsrP. Catalyzes the first step in glycosylation by transferring N-acetylglucosamine from UDP-GlcNAc to serine residues in PsrP (PubMed:24936067, PubMed:28246170). Part of the accessory SecA2/SecY2 system specifically required to export serine-rich repeat cell wall proteins encoded upstream in the same operon (Probable). The GtfA-GtfB complex adds GlcNAc from UDP-GlcNAc to PsrP (experimentally characterized with truncated PsrP-SSR1 constructs); this subunit alone has weak N-acetylglucosaminyl transferase activity that is 10-fold stimulated by GtfB. The complex requires at least a 25 residue-long peptide for activity; the in vitro assay has only been seen to glycosylate Ser residues (PubMed:24936067). The alpha linkage was shown in L.reuteri.[UniProtKB:A0A0S4NM89]<ref>PMID:24936067</ref> <ref>PMID:28246170</ref> <ref>PMID:16861665</ref>		[https://www.uniprot.org/uniprot/GTFA_STRPN GTFA_STRPN] Required for the polymorphic O-glycosylation of serine-rich repeat protein PsrP. Catalyzes the first step in glycosylation by transferring N-acetylglucosamine from UDP-GlcNAc to serine residues in PsrP (PubMed:24936067, PubMed:28246170). Part of the accessory SecA2/SecY2 system specifically required to export serine-rich repeat cell wall proteins encoded upstream in the same operon (Probable). The GtfA-GtfB complex adds GlcNAc from UDP-GlcNAc to PsrP (experimentally characterized with truncated PsrP-SSR1 constructs); this subunit alone has weak N-acetylglucosaminyl transferase activity that is 10-fold stimulated by GtfB. The complex requires at least a 25 residue-long peptide for activity; the in vitro assay has only been seen to glycosylate Ser residues (PubMed:24936067). The alpha linkage was shown in L.reuteri.[UniProtKB:A0A0S4NM89]<ref>PMID:24936067</ref> <ref>PMID:28246170</ref> <ref>PMID:16861665</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Protein glycosylation catalyzed by the O-GlcNAc transferase (OGT) plays a critical role in various biological processes. In Streptococcus pneumoniae, the core enzyme GtfA and co-activator GtfB form an OGT complex to glycosylate the serine-rich repeat (SRR) of adhesin PsrP (pneumococcal serine-rich repeat protein), which is involved in the infection and pathogenesis. Here we report the 2.0 A crystal structure of GtfA, revealing a beta-meander add-on domain beyond the catalytic domain. It represents a novel add-on domain, which is distinct from the all-alpha-tetratricopeptide repeats in the only two structure-known OGTs. Structural analyses combined with binding assays indicate that this add-on domain contributes to forming an active GtfA-GtfB complex and recognizing the acceptor protein. In addition, the in vitro glycosylation system enables us to map the O-linkages to the serine residues within the first SRR of PsrP. These findings suggest that fusion with an add-on domain might be a universal mechanism for diverse OGTs that recognize varying acceptor proteins/peptides.
-
-	Structure of a Novel O-Linked N-Acetyl-d-glucosamine (O-GlcNAc) Transferase, GtfA, Reveals Insights into the Glycosylation of Pneumococcal Serine-rich Repeat Adhesins.,Shi WW, Jiang YL, Zhu F, Yang YH, Shao QY, Yang HB, Ren YM, Wu H, Chen Y, Zhou CZ J Biol Chem. 2014 Jul 25;289(30):20898-20907. Epub 2014 Jun 16. PMID:24936067<ref>PMID:24936067</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4pqg" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 07:24, 8 February 2023

OCA — Wed, 08 Feb 2023 07:24:34 GMT

←Older revision		Revision as of 07:24, 8 February 2023
Line 3:		Line 3:
	<StructureSection load='4pqg' size='340' side='right'caption='[[4pqg]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4pqg' size='340' side='right'caption='[[4pqg]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4pqg]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Strpn Strpn~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PQG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4PQG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4pqg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PQG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PQG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gtf1, SP_1758 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=170187 STRPN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pqg OCA], [https://pdbe.org/4pqg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pqg RCSB], [https://www.ebi.ac.uk/pdbsum/4pqg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pqg ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4pqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pqg OCA], [~~http~~://pdbe.org/4pqg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4pqg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4pqg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4pqg ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~Q97P84_STRPN Q97P84_STRPN~~]~~] An~~ N-~~acetylglucosaminyl transferase that is part~~ of the accessory SecA2/SecY2 system specifically required to export serine-rich repeat cell wall proteins ~~usually~~ encoded upstream in the same operon (~~By similarity~~).~~[HAMAP~~-~~Rule~~:~~MF_01472~~]	+	[https://www.uniprot.org/uniprot/GTFA_STRPN GTFA_STRPN] Required for the polymorphic O-glycosylation of serine-rich repeat protein PsrP. Catalyzes the first step in glycosylation by transferring N-acetylglucosamine from UDP-GlcNAc to serine residues in PsrP (PubMed:24936067, PubMed:28246170). Part of the accessory SecA2/SecY2 system specifically required to export serine-rich repeat cell wall proteins encoded upstream in the same operon (Probable). The GtfA-GtfB complex adds GlcNAc from UDP-GlcNAc to PsrP (experimentally characterized with truncated PsrP-SSR1 constructs); this subunit alone has weak N-acetylglucosaminyl transferase activity that is 10-fold stimulated by GtfB. The complex requires at least a 25 residue-long peptide for activity; the in vitro assay has only been seen to glycosylate Ser residues (PubMed:24936067). The alpha linkage was shown in L.reuteri.[UniProtKB:A0A0S4NM89]<ref>PMID:24936067</ref> <ref>PMID:28246170</ref> <ref>PMID:16861665</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 20:

	==See Also==		==See Also==
-	*[[Glycosyltransferase\|Glycosyltransferase]]	+	*[[Glycosyltransferase 3D structures\|Glycosyltransferase 3D structures]]
-	*[[O-GlcNAc transferase\|O-GlcNAc transferase]]	+	*[[O-GlcNAc transferase 3D structures\|O-GlcNAc transferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Strpn~~]]	+	[[Category: Streptococcus pneumoniae TIGR4]]
-	[[Category: Chen, Y]]	+	[[Category: Chen Y]]
-	[[Category: Jiang~~, Y L~~]]	+	[[Category: Jiang YL]]
-	[[Category: Ren~~, Y M~~]]	+	[[Category: Ren YM]]
-	[[Category: Shi~~, W W~~]]	+	[[Category: Shi WW]]
-	[[Category: Wu, H]]	+	[[Category: Wu H]]
-	[[Category: Yang~~, Y H~~]]	+	[[Category: Yang YH]]
-	[[Category: Zhou~~, C Z~~]]	+	[[Category: Zhou CZ]]
-	[[Category: Zhu, F]]	+	[[Category: Zhu F]]
-	~~[[Category: Gt-b fold]]~~	+
-	~~[[Category: Gtfb]]~~	+
-	~~[[Category: O-glcnac transferase]]~~	+
-	~~[[Category: Rossmann fold]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:41, 17 April 2019

OCA — Wed, 17 Apr 2019 07:41:13 GMT

←Older revision		Revision as of 07:41, 17 April 2019
Line 1:		Line 1:

	==Crystal structure of the pneumococcal O-GlcNAc transferase GtfA in complex with UDP and GlcNAc==		==Crystal structure of the pneumococcal O-GlcNAc transferase GtfA in complex with UDP and GlcNAc==
-	<StructureSection load='4pqg' size='340' side='right' caption='[[4pqg]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='4pqg' size='340' side='right'caption='[[4pqg]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4pqg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PQG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PQG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4pqg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Strpn Strpn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PQG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PQG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gtf1, SP_1758 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=170187 STRPN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pqg OCA], [http://pdbe.org/4pqg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pqg RCSB], [http://www.ebi.ac.uk/pdbsum/4pqg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pqg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pqg OCA], [http://pdbe.org/4pqg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pqg RCSB], [http://www.ebi.ac.uk/pdbsum/4pqg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pqg ProSAT]</span></td></tr>
	</table>		</table>
Line 26:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Strpn]]
	[[Category: Chen, Y]]		[[Category: Chen, Y]]
	[[Category: Jiang, Y L]]		[[Category: Jiang, Y L]]

OCA at 03:02, 5 August 2016

OCA — Fri, 05 Aug 2016 03:02:10 GMT

←Older revision		Revision as of 03:02, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the pneumococcal O-GlcNAc transferase GtfA in complex with UDP and GlcNAc==		==Crystal structure of the pneumococcal O-GlcNAc transferase GtfA in complex with UDP and GlcNAc==
	<StructureSection load='4pqg' size='340' side='right' caption='[[4pqg]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4pqg' size='340' side='right' caption='[[4pqg]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4pqg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PQG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PQG FirstGlance]. <br>		<table><tr><td colspan='2'>[[4pqg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PQG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PQG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pqg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pqg RCSB], [http://www.ebi.ac.uk/pdbsum/4pqg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pqg OCA], [http://pdbe.org/4pqg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pqg RCSB], [http://www.ebi.ac.uk/pdbsum/4pqg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pqg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4pqg" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glycosyltransferase\|Glycosyltransferase]]
		+	*[[O-GlcNAc transferase\|O-GlcNAc transferase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 22:19, 24 December 2014

OCA — Wed, 24 Dec 2014 22:19:12 GMT

←Older revision		Revision as of 22:19, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4pqg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PQG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PQG FirstGlance]. <br>		<table><tr><td colspan='2'>[[4pqg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PQG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PQG FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pqg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pqg RCSB], [http://www.ebi.ac.uk/pdbsum/4pqg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pqg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pqg RCSB], [http://www.ebi.ac.uk/pdbsum/4pqg PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q97P84_STRPN Q97P84_STRPN]] An N-acetylglucosaminyl transferase that is part of the accessory SecA2/SecY2 system specifically required to export serine-rich repeat cell wall proteins usually encoded upstream in the same operon (By similarity).[HAMAP-Rule:MF_01472]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Chen, Y.]]	+	[[Category: Chen, Y]]
-	[[Category: Jiang, Y L.]]	+	[[Category: Jiang, Y L]]
-	[[Category: Ren, Y M.]]	+	[[Category: Ren, Y M]]
-	[[Category: Shi, W W.]]	+	[[Category: Shi, W W]]
-	[[Category: Wu, H.]]	+	[[Category: Wu, H]]
-	[[Category: Yang, Y H.]]	+	[[Category: Yang, Y H]]
-	[[Category: Zhou, C Z.]]	+	[[Category: Zhou, C Z]]
-	[[Category: Zhu, F.]]	+	[[Category: Zhu, F]]
	[[Category: Gt-b fold]]		[[Category: Gt-b fold]]
	[[Category: Gtfb]]		[[Category: Gtfb]]

OCA at 02:29, 7 August 2014

OCA — Thu, 07 Aug 2014 02:29:10 GMT

←Older revision		Revision as of 02:29, 7 August 2014
Line 6:		Line 6:
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pqg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pqg RCSB], [http://www.ebi.ac.uk/pdbsum/4pqg PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pqg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pqg RCSB], [http://www.ebi.ac.uk/pdbsum/4pqg PDBsum]</span></td></tr>
	<table>		<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Protein glycosylation catalyzed by the O-GlcNAc transferase (OGT) plays a critical role in various biological processes. In Streptococcus pneumoniae, the core enzyme GtfA and co-activator GtfB form an OGT complex to glycosylate the serine-rich repeat (SRR) of adhesin PsrP (pneumococcal serine-rich repeat protein), which is involved in the infection and pathogenesis. Here we report the 2.0 A crystal structure of GtfA, revealing a beta-meander add-on domain beyond the catalytic domain. It represents a novel add-on domain, which is distinct from the all-alpha-tetratricopeptide repeats in the only two structure-known OGTs. Structural analyses combined with binding assays indicate that this add-on domain contributes to forming an active GtfA-GtfB complex and recognizing the acceptor protein. In addition, the in vitro glycosylation system enables us to map the O-linkages to the serine residues within the first SRR of PsrP. These findings suggest that fusion with an add-on domain might be a universal mechanism for diverse OGTs that recognize varying acceptor proteins/peptides.
		+
		+	Structure of a Novel O-Linked N-Acetyl-d-glucosamine (O-GlcNAc) Transferase, GtfA, Reveals Insights into the Glycosylation of Pneumococcal Serine-rich Repeat Adhesins.,Shi WW, Jiang YL, Zhu F, Yang YH, Shao QY, Yang HB, Ren YM, Wu H, Chen Y, Zhou CZ J Biol Chem. 2014 Jul 25;289(30):20898-20907. Epub 2014 Jun 16. PMID:24936067<ref>PMID:24936067</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:20, 18 June 2014

OCA — Wed, 18 Jun 2014 08:20:41 GMT

←Older revision		Revision as of 08:20, 18 June 2014
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-	'''~~Unreleased structure~~'''	+	==Crystal structure of the pneumococcal O-GlcNAc transferase GtfA in complex with UDP and GlcNAc==
-		+	<StructureSection load='4pqg' size='340' side='right' caption='[[4pqg]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~The entry~~ 4pqg is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4pqg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PQG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PQG FirstGlance]. <br>
-	~~Authors~~: ~~Shi~~, W.W., ~~Jiang, Y~~.L., ~~Zhu, F~~., ~~Yang, Y~~.H., ~~Wu, H~~., Ren, Y.M., ~~Chen~~, Y., Zhou, C.Z.	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene><br>
-		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pqg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pqg RCSB], [http://www.ebi.ac.uk/pdbsum/4pqg PDBsum]</span></td></tr>
-	~~Description~~: ~~Crystal structure of the pneumococcal~~ O-~~GlcNAc~~ transferase ~~GtfA in complex with UDP and GlcNAc~~	+	<table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chen, Y.]]
		+	[[Category: Jiang, Y L.]]
		+	[[Category: Ren, Y M.]]
		+	[[Category: Shi, W W.]]
		+	[[Category: Wu, H.]]
		+	[[Category: Yang, Y H.]]
		+	[[Category: Zhou, C Z.]]
		+	[[Category: Zhu, F.]]
		+	[[Category: Gt-b fold]]
		+	[[Category: Gtfb]]
		+	[[Category: O-glcnac transferase]]
		+	[[Category: Rossmann fold]]
		+	[[Category: Transferase]]

OCA at 06:33, 30 April 2014

OCA — Wed, 30 Apr 2014 06:33:33 GMT

←Older revision		Revision as of 06:33, 30 April 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4pqg is ON HOLD	+	The entry 4pqg is ON HOLD until Paper Publication

	Authors: Shi, W.W., Jiang, Y.L., Zhu, F., Yang, Y.H., Wu, H., Ren, Y.M., Chen, Y., Zhou, C.Z.		Authors: Shi, W.W., Jiang, Y.L., Zhu, F., Yang, Y.H., Wu, H., Ren, Y.M., Chen, Y., Zhou, C.Z.

	Description: Crystal structure of the pneumococcal O-GlcNAc transferase GtfA in complex with UDP and GlcNAc		Description: Crystal structure of the pneumococcal O-GlcNAc transferase GtfA in complex with UDP and GlcNAc

OCA: Protected "4pqg" [edit=sysop:move=sysop]

OCA — Wed, 12 Mar 2014 12:35:23 GMT

Protected "4pqg" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:35, 12 March 2014

OCA: New page: '''Unreleased structure''' The entry 4pqg is ON HOLD Authors: Shi, W.W., Jiang, Y.L., Zhu, F., Yang, Y.H., Wu, H., Ren, Y.M., Chen, Y., Zhou, C.Z. Description: Crystal structure of the...

OCA — Wed, 12 Mar 2014 12:35:22 GMT

New page: '''Unreleased structure''' The entry 4pqg is ON HOLD Authors: Shi, W.W., Jiang, Y.L., Zhu, F., Yang, Y.H., Wu, H., Ren, Y.M., Chen, Y., Zhou, C.Z. Description: Crystal structure of the...

New page

'''Unreleased structure'''

The entry 4pqg is ON HOLD

Authors: Shi, W.W., Jiang, Y.L., Zhu, F., Yang, Y.H., Wu, H., Ren, Y.M., Chen, Y., Zhou, C.Z.

Description: Crystal structure of the pneumococcal O-GlcNAc transferase GtfA in complex with UDP and GlcNAc