4ptw - Revision history

OCA at 15:00, 8 November 2023

OCA — Wed, 08 Nov 2023 15:00:58 GMT

←Older revision		Revision as of 15:00, 8 November 2023
Line 1:		Line 1:

	==Halothermothrix orenii beta-glucosidase A, 2-deoxy-2-fluoro-glucose complex==		==Halothermothrix orenii beta-glucosidase A, 2-deoxy-2-fluoro-glucose complex==
-	<StructureSection load='4ptw' size='340' side='right'caption='[[4ptw]]' scene=''>	+	<StructureSection load='4ptw' size='340' side='right'caption='[[4ptw]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PTW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PTW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ptw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Halothermothrix_orenii_H_168 Halothermothrix orenii H 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PTW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PTW FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ptw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ptw OCA], [https://pdbe.org/4ptw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ptw RCSB], [https://www.ebi.ac.uk/pdbsum/4ptw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ptw ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G2F:2-DEOXY-2-FLUORO-ALPHA-D-GLUCOPYRANOSE'>G2F</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ptw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ptw OCA], [https://pdbe.org/4ptw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ptw RCSB], [https://www.ebi.ac.uk/pdbsum/4ptw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ptw ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B8CYA8_HALOH B8CYA8_HALOH]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lactose is a major disaccharide by-product from the dairy industries, and production of whey alone amounts to about 200 million tons globally each year. Thus, it is of particular interest to identify improved enzymatic processes for lactose utilization. Microbial beta-glucosidases (BGL) with significant beta-galactosidase (BGAL) activity can be used to convert lactose to glucose (Glc) and galactose (Gal), and most retaining BGLs also synthesize more complex sugars from the monosaccharides by transglycosylation, such as galacto-oligosaccharides (GOS), which are prebiotic compounds that stimulate growth of beneficial gut bacteria. In this work, a BGL from the thermophilic and halophilic bacterium Halothermothrix orenii, HoBGLA, was characterized biochemically and structurally. It is an unspecific beta-glucosidase with mixed activities for different substrates and prominent activity with various galactosidases such as lactose. We show that HoBGLA is an attractive candidate for industrial lactose conversion based on its high activity and stability within a broad pH range (4.5-7.5), with maximal beta-galactosidase activity at pH 6.0. The temperature optimum is in the range of 65-70 degrees C, and HoBGLA also shows excellent thermostability at this temperature range. The main GOS products from HoBGLA transgalactosylation are beta-D-Galp-(1-->6)-D-Lac (6GALA) and beta-D-Galp-(1-->3)-D-Lac (3GALA), indicating that D-lactose is a better galactosyl acceptor than either of the monosaccharides. To evaluate ligand binding and guide GOS modeling, crystal structures of HoBGLA were determined in complex with thiocellobiose, 2-deoxy-2-fluoro-D-glucose and glucose. The two major GOS products, 3GALA and 6GALA, were modeled in the substrate-binding cleft of wild-type HoBGLA and shown to be favorably accommodated.
		+
		+	Biochemical and structural characterization of a thermostable beta-glucosidase from Halothermothrix orenii for galacto-oligosaccharide synthesis.,Hassan N, Nguyen TH, Intanon M, Kori LD, Patel BK, Haltrich D, Divne C, Tan TC Appl Microbiol Biotechnol. 2014 Aug 31. PMID:25173693<ref>PMID:25173693</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4ptw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Halothermothrix orenii H 168]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Divne C]]		[[Category: Divne C]]

OCA at 10:52, 31 August 2022

OCA — Wed, 31 Aug 2022 10:52:36 GMT

←Older revision		Revision as of 10:52, 31 August 2022
Line 1:		Line 1:

	==Halothermothrix orenii beta-glucosidase A, 2-deoxy-2-fluoro-glucose complex==		==Halothermothrix orenii beta-glucosidase A, 2-deoxy-2-fluoro-glucose complex==
-	<StructureSection load='4ptw' size='340' side='right'caption='[[4ptw]]~~, [[Resolution\|resolution]] 2.00Å~~' scene=''>	+	<StructureSection load='4ptw' size='340' side='right'caption='[[4ptw]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[4ptw]] is a 2 chain structure.~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PTW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PTW FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PTW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PTW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G2F:2-DEOXY-2-FLUORO-ALPHA-D-GLUCOPYRANOSE'>G2F</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ptw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ptw OCA], [https://pdbe.org/4ptw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ptw RCSB], [https://www.ebi.ac.uk/pdbsum/4ptw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ptw ProSAT]</span></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ta9\|3ta9]], [[4ptv\|4ptv]], [[4ptx\|4ptx]]</div></td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ptw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ptw OCA], [https://pdbe.org/4ptw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ptw RCSB], [https://www.ebi.ac.uk/pdbsum/4ptw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ptw ProSAT]</span></td></tr>	+
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Lactose is a major disaccharide by-product from the dairy industries, and production of whey alone amounts to about 200 million tons globally each year. Thus, it is of particular interest to identify improved enzymatic processes for lactose utilization. Microbial beta-glucosidases (BGL) with significant beta-galactosidase (BGAL) activity can be used to convert lactose to glucose (Glc) and galactose (Gal), and most retaining BGLs also synthesize more complex sugars from the monosaccharides by transglycosylation, such as galacto-oligosaccharides (GOS), which are prebiotic compounds that stimulate growth of beneficial gut bacteria. In this work, a BGL from the thermophilic and halophilic bacterium Halothermothrix orenii, HoBGLA, was characterized biochemically and structurally. It is an unspecific beta-glucosidase with mixed activities for different substrates and prominent activity with various galactosidases such as lactose. We show that HoBGLA is an attractive candidate for industrial lactose conversion based on its high activity and stability within a broad pH range (4.5-7.5), with maximal beta-galactosidase activity at pH 6.0. The temperature optimum is in the range of 65-70 degrees C, and HoBGLA also shows excellent thermostability at this temperature range. The main GOS products from HoBGLA transgalactosylation are beta-D-Galp-(1-->6)-D-Lac (6GALA) and beta-D-Galp-(1-->3)-D-Lac (3GALA), indicating that D-lactose is a better galactosyl acceptor than either of the monosaccharides. To evaluate ligand binding and guide GOS modeling, crystal structures of HoBGLA were determined in complex with thiocellobiose, 2-deoxy-2-fluoro-D-glucose and glucose. The two major GOS products, 3GALA and 6GALA, were modeled in the substrate-binding cleft of wild-type HoBGLA and shown to be favorably accommodated.
-
-	Biochemical and structural characterization of a thermostable beta-glucosidase from Halothermothrix orenii for galacto-oligosaccharide synthesis.,Hassan N, Nguyen TH, Intanon M, Kori LD, Patel BK, Haltrich D, Divne C, Tan TC Appl Microbiol Biotechnol. 2014 Aug 31. PMID:25173693<ref>PMID:25173693</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4ptw" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Beta-glucosidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Divne, C]]	+	[[Category: Divne C]]
-	[[Category: Haltrich, D]]	+	[[Category: Haltrich D]]
-	[[Category: Hassan, N]]	+	[[Category: Hassan N]]
-	[[Category: Kori~~, L D~~]]	+	[[Category: Kori LD]]
-	[[Category: Nguyen~~, T H~~]]	+	[[Category: Nguyen TH]]
-	[[Category: Patel~~, B K.C~~]]	+	[[Category: Patel BKC]]
-	[[Category: Tan~~, T C]]~~	+	[[Category: Tan TC]]
-	~~[[Category: Glycoside hydrolase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Tim barrel~~]]	+

OCA at 05:06, 25 August 2022

OCA — Thu, 25 Aug 2022 05:06:00 GMT

←Older revision		Revision as of 05:06, 25 August 2022
Line 3:		Line 3:
	<StructureSection load='4ptw' size='340' side='right'caption='[[4ptw]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4ptw' size='340' side='right'caption='[[4ptw]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ptw]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Haloh Haloh]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PTW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4PTW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ptw]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PTW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PTW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2F:2-DEOXY-2-FLUORO-ALPHA-D-GLUCOPYRANOSE'>G2F</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G2F:2-DEOXY-2-FLUORO-ALPHA-D-GLUCOPYRANOSE'>G2F</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ta9\|3ta9]], [[4ptv\|4ptv]], [[4ptx\|4ptx]]</td>~~</tr>~~	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3ta9\|3ta9]], [[4ptv\|4ptv]], [[4ptx\|4ptx]]</div></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bgla, Hore_15280 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=373903 HALOH])~~</td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ptw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ptw OCA], [https://pdbe.org/4ptw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ptw RCSB], [https://www.ebi.ac.uk/pdbsum/4ptw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ptw ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ptw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ptw OCA], [~~http~~://pdbe.org/4ptw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ptw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ptw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ptw ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 24:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Beta-glucosidase]]		[[Category: Beta-glucosidase]]
-	[[Category: Haloh]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Divne, C]]		[[Category: Divne, C]]

OCA at 07:53, 17 April 2019

OCA — Wed, 17 Apr 2019 07:53:06 GMT

←Older revision		Revision as of 07:53, 17 April 2019
Line 1:		Line 1:

	==Halothermothrix orenii beta-glucosidase A, 2-deoxy-2-fluoro-glucose complex==		==Halothermothrix orenii beta-glucosidase A, 2-deoxy-2-fluoro-glucose complex==
-	<StructureSection load='4ptw' size='340' side='right' caption='[[4ptw]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='4ptw' size='340' side='right'caption='[[4ptw]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ptw]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PTW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PTW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ptw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Haloh Haloh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PTW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PTW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2F:2-DEOXY-2-FLUORO-ALPHA-D-GLUCOPYRANOSE'>G2F</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2F:2-DEOXY-2-FLUORO-ALPHA-D-GLUCOPYRANOSE'>G2F</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ta9\|3ta9]], [[4ptv\|4ptv]], [[4ptx\|4ptx]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ta9\|3ta9]], [[4ptv\|4ptv]], [[4ptx\|4ptx]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bgla, Hore_15280 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=373903 HALOH])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ptw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ptw OCA], [http://pdbe.org/4ptw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ptw RCSB], [http://www.ebi.ac.uk/pdbsum/4ptw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ptw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ptw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ptw OCA], [http://pdbe.org/4ptw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ptw RCSB], [http://www.ebi.ac.uk/pdbsum/4ptw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ptw ProSAT]</span></td></tr>
Line 23:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Beta-glucosidase]]		[[Category: Beta-glucosidase]]
		+	[[Category: Haloh]]
		+	[[Category: Large Structures]]
	[[Category: Divne, C]]		[[Category: Divne, C]]
	[[Category: Haltrich, D]]		[[Category: Haltrich, D]]

OCA at 19:10, 11 August 2016

OCA — Thu, 11 Aug 2016 19:10:50 GMT

←Older revision		Revision as of 19:10, 11 August 2016
Line 1:		Line 1:
		+
	==Halothermothrix orenii beta-glucosidase A, 2-deoxy-2-fluoro-glucose complex==		==Halothermothrix orenii beta-glucosidase A, 2-deoxy-2-fluoro-glucose complex==
	<StructureSection load='4ptw' size='340' side='right' caption='[[4ptw]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4ptw' size='340' side='right' caption='[[4ptw]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ta9\|3ta9]], [[4ptv\|4ptv]], [[4ptx\|4ptx]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ta9\|3ta9]], [[4ptv\|4ptv]], [[4ptx\|4ptx]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ptw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ptw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ptw RCSB], [http://www.ebi.ac.uk/pdbsum/4ptw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ptw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ptw OCA], [http://pdbe.org/4ptw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ptw RCSB], [http://www.ebi.ac.uk/pdbsum/4ptw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ptw ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4ptw" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:30, 20 January 2015

OCA — Tue, 20 Jan 2015 09:30:51 GMT

←Older revision		Revision as of 09:30, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ptw]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PTW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PTW FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ptw]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PTW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PTW FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2F:2-DEOXY-2-FLUORO-ALPHA-D-GLUCOPYRANOSE'>G2F</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2F:2-DEOXY-2-FLUORO-ALPHA-D-GLUCOPYRANOSE'>G2F</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ta9\|3ta9]], [[4ptv\|4ptv]], [[4ptx\|4ptx]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ta9\|3ta9]], [[4ptv\|4ptv]], [[4ptx\|4ptx]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ptw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ptw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ptw RCSB], [http://www.ebi.ac.uk/pdbsum/4ptw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ptw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ptw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ptw RCSB], [http://www.ebi.ac.uk/pdbsum/4ptw PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Beta-glucosidase]]		[[Category: Beta-glucosidase]]
-	[[Category: Divne, C.]]	+	[[Category: Divne, C]]
-	[[Category: Haltrich, D.]]	+	[[Category: Haltrich, D]]
-	[[Category: Hassan, N.]]	+	[[Category: Hassan, N]]
-	[[Category: Kori, L D.]]	+	[[Category: Kori, L D]]
-	[[Category: Nguyen, T H.]]	+	[[Category: Nguyen, T H]]
-	[[Category: Patel, B K.C.]]	+	[[Category: Patel, B K.C]]
-	[[Category: Tan, T C~~.]]~~	+	[[Category: Tan, T C]]
-	~~[[Category: Beta-glucosidase~~]]	+
	[[Category: Glycoside hydrolase]]		[[Category: Glycoside hydrolase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Tim barrel]]		[[Category: Tim barrel]]

OCA at 10:20, 24 September 2014

OCA — Wed, 24 Sep 2014 10:20:11 GMT

←Older revision		Revision as of 10:20, 24 September 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Halothermothrix orenii beta-glucosidase A, 2-deoxy-2-fluoro-glucose complex==
		+	<StructureSection load='4ptw' size='340' side='right' caption='[[4ptw]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4ptw]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PTW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PTW FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2F:2-DEOXY-2-FLUORO-ALPHA-D-GLUCOPYRANOSE'>G2F</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3ta9\|3ta9]], [[4ptv\|4ptv]], [[4ptx\|4ptx]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ptw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ptw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ptw RCSB], [http://www.ebi.ac.uk/pdbsum/4ptw PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lactose is a major disaccharide by-product from the dairy industries, and production of whey alone amounts to about 200 million tons globally each year. Thus, it is of particular interest to identify improved enzymatic processes for lactose utilization. Microbial beta-glucosidases (BGL) with significant beta-galactosidase (BGAL) activity can be used to convert lactose to glucose (Glc) and galactose (Gal), and most retaining BGLs also synthesize more complex sugars from the monosaccharides by transglycosylation, such as galacto-oligosaccharides (GOS), which are prebiotic compounds that stimulate growth of beneficial gut bacteria. In this work, a BGL from the thermophilic and halophilic bacterium Halothermothrix orenii, HoBGLA, was characterized biochemically and structurally. It is an unspecific beta-glucosidase with mixed activities for different substrates and prominent activity with various galactosidases such as lactose. We show that HoBGLA is an attractive candidate for industrial lactose conversion based on its high activity and stability within a broad pH range (4.5-7.5), with maximal beta-galactosidase activity at pH 6.0. The temperature optimum is in the range of 65-70 degrees C, and HoBGLA also shows excellent thermostability at this temperature range. The main GOS products from HoBGLA transgalactosylation are beta-D-Galp-(1-->6)-D-Lac (6GALA) and beta-D-Galp-(1-->3)-D-Lac (3GALA), indicating that D-lactose is a better galactosyl acceptor than either of the monosaccharides. To evaluate ligand binding and guide GOS modeling, crystal structures of HoBGLA were determined in complex with thiocellobiose, 2-deoxy-2-fluoro-D-glucose and glucose. The two major GOS products, 3GALA and 6GALA, were modeled in the substrate-binding cleft of wild-type HoBGLA and shown to be favorably accommodated.

-	~~The entry 4ptw is ON HOLD until Paper Publication~~	+	Biochemical and structural characterization of a thermostable beta-glucosidase from Halothermothrix orenii for galacto-oligosaccharide synthesis.,Hassan N, Nguyen TH, Intanon M, Kori LD, Patel BK, Haltrich D, Divne C, Tan TC Appl Microbiol Biotechnol. 2014 Aug 31. PMID:25173693<ref>PMID:25173693</ref>

-	~~Authors~~: ~~Hassan~~, N., ~~Nguyen, T.H~~., Kori, L.D., Patel, B.K.C.~~, Haltrich, D., Divne, C.,~~ Tan, T.C.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Halothermothrix orenii beta~~-glucosidase ~~A, 2-deoxy-2-fluoro-glucose complex~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Beta-glucosidase]]
		+	[[Category: Divne, C.]]
		+	[[Category: Haltrich, D.]]
		+	[[Category: Hassan, N.]]
		+	[[Category: Kori, L D.]]
		+	[[Category: Nguyen, T H.]]
		+	[[Category: Patel, B K.C.]]
		+	[[Category: Tan, T C.]]
		+	[[Category: Beta-glucosidase]]
		+	[[Category: Glycoside hydrolase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Tim barrel]]

OCA at 07:27, 23 April 2014

OCA — Wed, 23 Apr 2014 07:27:24 GMT

←Older revision		Revision as of 07:27, 23 April 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4ptw is ON HOLD	+	The entry 4ptw is ON HOLD until Paper Publication

	Authors: Hassan, N., Nguyen, T.H., Kori, L.D., Patel, B.K.C., Haltrich, D., Divne, C., Tan, T.C.		Authors: Hassan, N., Nguyen, T.H., Kori, L.D., Patel, B.K.C., Haltrich, D., Divne, C., Tan, T.C.

	Description: Halothermothrix orenii beta-glucosidase A, 2-deoxy-2-fluoro-glucose complex		Description: Halothermothrix orenii beta-glucosidase A, 2-deoxy-2-fluoro-glucose complex

OCA: Protected "4ptw" [edit=sysop:move=sysop]

OCA — Wed, 19 Mar 2014 07:43:44 GMT

Protected "4ptw" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:43, 19 March 2014

OCA: New page: '''Unreleased structure''' The entry 4ptw is ON HOLD Authors: Hassan, N., Nguyen, T.H., Kori, L.D., Patel, B.K.C., Haltrich, D., Divne, C., Tan, T.C. Description: Halothermothrix oreni...

OCA — Wed, 19 Mar 2014 07:43:43 GMT

New page: '''Unreleased structure''' The entry 4ptw is ON HOLD Authors: Hassan, N., Nguyen, T.H., Kori, L.D., Patel, B.K.C., Haltrich, D., Divne, C., Tan, T.C. Description: Halothermothrix oreni...

New page

'''Unreleased structure'''

The entry 4ptw is ON HOLD

Authors: Hassan, N., Nguyen, T.H., Kori, L.D., Patel, B.K.C., Haltrich, D., Divne, C., Tan, T.C.

Description: Halothermothrix orenii beta-glucosidase A, 2-deoxy-2-fluoro-glucose complex