4pu0 - Revision history

OCA at 12:43, 1 March 2024

OCA — Fri, 01 Mar 2024 12:43:05 GMT

←Older revision		Revision as of 12:43, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4pu0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PU0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PU0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4pu0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PU0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PU0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3018Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pu0 OCA], [https://pdbe.org/4pu0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pu0 RCSB], [https://www.ebi.ac.uk/pdbsum/4pu0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pu0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pu0 OCA], [https://pdbe.org/4pu0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pu0 RCSB], [https://www.ebi.ac.uk/pdbsum/4pu0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pu0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/SSUE_ECOLI SSUE_ECOLI] Catalyzes an NADPH-dependent reduction of FMN, but is also able to reduce FAD or riboflavin.		[https://www.uniprot.org/uniprot/SSUE_ECOLI SSUE_ECOLI] Catalyzes an NADPH-dependent reduction of FMN, but is also able to reduce FAD or riboflavin.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The Escherichia coli sulfur starvation utilization (ssu) operon includes a two-component monooxygenase system consisting of a nicotinamide adenine dinucleotide phosphate (NADPH)-dependent flavin mononucleotide (FMN) reductase, SsuE, and a monooxygenase, SsuD. SsuE is part of the flavodoxin-like superfamily, and we report here the crystal structures of its apo, FMN-bound, and FMNH2-bound forms at approximately 2 A resolution. In the crystals, SsuE forms a tetramer that is a dimer of dimers similar to those seen for homologous FMN reductases, quinone reductases, and the WrbA family of enzymes. A pi-helix present at the tetramer building interface is unique to the reductases from two-component monooxygenase systems. Analytical ultracentrifugation studies of SsuE confirm a dimer-tetramer equilibrium exists in solution, with FMN binding favoring the dimer. As the active site includes residues from both subunits, at least a dimeric association is required for the function of SsuE. The structures show that one FMN binds tightly in a deeply held site, which makes available a second binding site, in which either a second FMN or the nicotinamide of NADPH can bind. The FMNH2-bound structure shows subtle changes consistent with its binding being weaker than that of FMN. Combining this information with published kinetic studies, we propose a general catalytic cycle for two-component reductases of the flavodoxin-like superfamily, by which the enzyme can potentially provide FMNH2 to its partner monooxygenase by different routes depending on the FMN concentration and the presence of a partner monooxygenase.
-
-	Crystal Structure of Escherichia coli SsuE: Defining a General Catalytic Cycle for FMN Reductases of the Flavodoxin-like Superfamily.,Driggers CM, Dayal PV, Ellis HR, Karplus PA Biochemistry. 2014 Jun 3;53(21):3509-19. doi: 10.1021/bi500314f. Epub 2014 May, 21. PMID:24816272<ref>PMID:24816272</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4pu0" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:30, 8 February 2023

OCA — Wed, 08 Feb 2023 07:30:46 GMT

←Older revision		Revision as of 07:30, 8 February 2023
Line 3:		Line 3:
	<StructureSection load='4pu0' size='340' side='right'caption='[[4pu0]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4pu0' size='340' side='right'caption='[[4pu0]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4pu0]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PU0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4PU0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4pu0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PU0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PU0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pty\|4pty]], [[4ptz\|4ptz]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pu0 OCA], [https://pdbe.org/4pu0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pu0 RCSB], [https://www.ebi.ac.uk/pdbsum/4pu0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pu0 ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ssuE, ycbP, b0937, JW0920 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/FMN_reductase_(NADPH) FMN reductase (NADPH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.38 1.5.1.38] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4pu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pu0 OCA], [~~http~~://pdbe.org/4pu0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4pu0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4pu0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4pu0 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SSUE_ECOLI SSUE_ECOLI]] Catalyzes an NADPH-dependent reduction of FMN, but is also able to reduce FAD or riboflavin.	+	[https://www.uniprot.org/uniprot/SSUE_ECOLI SSUE_ECOLI] Catalyzes an NADPH-dependent reduction of FMN, but is also able to reduce FAD or riboflavin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Driggers~~, C M~~]]	+	[[Category: Driggers CM]]
-	[[Category: Ellis~~, H R~~]]	+	[[Category: Ellis HR]]
-	[[Category: Karplus~~, P A]]~~	+	[[Category: Karplus PA]]
-	~~[[Category: Flavodoxin-like fold]]~~	+
-	~~[[Category: Nadph-dependent fmn reductase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Ssud~~]]	+

OCA at 07:44, 17 April 2019

OCA — Wed, 17 Apr 2019 07:44:21 GMT

←Older revision		Revision as of 07:44, 17 April 2019
Line 1:		Line 1:

	==Crystal structure of the Escherichia coli alkanesulfonate FMN reductase SsuE in FMNH2-bound form==		==Crystal structure of the Escherichia coli alkanesulfonate FMN reductase SsuE in FMNH2-bound form==
-	<StructureSection load='4pu0' size='340' side='right' caption='[[4pu0]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='4pu0' size='340' side='right'caption='[[4pu0]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4pu0]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PU0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PU0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4pu0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PU0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PU0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pty\|4pty]], [[4ptz\|4ptz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pty\|4pty]], [[4ptz\|4ptz]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ssuE, ycbP, b0937, JW0920 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/FMN_reductase_(NADPH) FMN reductase (NADPH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.38 1.5.1.38] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/FMN_reductase_(NADPH) FMN reductase (NADPH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.38 1.5.1.38] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pu0 OCA], [http://pdbe.org/4pu0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pu0 RCSB], [http://www.ebi.ac.uk/pdbsum/4pu0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pu0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pu0 OCA], [http://pdbe.org/4pu0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pu0 RCSB], [http://www.ebi.ac.uk/pdbsum/4pu0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pu0 ProSAT]</span></td></tr>
Line 24:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Driggers, C M]]		[[Category: Driggers, C M]]
	[[Category: Ellis, H R]]		[[Category: Ellis, H R]]

OCA at 11:24, 4 August 2016

OCA — Thu, 04 Aug 2016 11:24:21 GMT

←Older revision		Revision as of 11:24, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the Escherichia coli alkanesulfonate FMN reductase SsuE in FMNH2-bound form==		==Crystal structure of the Escherichia coli alkanesulfonate FMN reductase SsuE in FMNH2-bound form==
	<StructureSection load='4pu0' size='340' side='right' caption='[[4pu0]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4pu0' size='340' side='right' caption='[[4pu0]], [[Resolution\|resolution]] 2.30Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pty\|4pty]], [[4ptz\|4ptz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pty\|4pty]], [[4ptz\|4ptz]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/FMN_reductase_(NADPH) FMN reductase (NADPH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.38 1.5.1.38] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/FMN_reductase_(NADPH) FMN reductase (NADPH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.38 1.5.1.38] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pu0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pu0 RCSB], [http://www.ebi.ac.uk/pdbsum/4pu0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pu0 OCA], [http://pdbe.org/4pu0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pu0 RCSB], [http://www.ebi.ac.uk/pdbsum/4pu0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pu0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4pu0" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:49, 25 December 2014

OCA — Thu, 25 Dec 2014 08:49:12 GMT

←Older revision		Revision as of 08:49, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4pu0]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PU0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PU0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4pu0]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PU0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PU0 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pty\|4pty]], [[4ptz\|4ptz]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pty\|4pty]], [[4ptz\|4ptz]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/FMN_reductase_(NADPH) FMN reductase (NADPH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.38 1.5.1.38] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/FMN_reductase_(NADPH) FMN reductase (NADPH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.38 1.5.1.38] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pu0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pu0 RCSB], [http://www.ebi.ac.uk/pdbsum/4pu0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pu0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pu0 RCSB], [http://www.ebi.ac.uk/pdbsum/4pu0 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SSUE_ECOLI SSUE_ECOLI]] Catalyzes an NADPH-dependent reduction of FMN, but is also able to reduce FAD or riboflavin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Driggers, C M.]]	+	[[Category: Driggers, C M]]
-	[[Category: Ellis, H R.]]	+	[[Category: Ellis, H R]]
-	[[Category: Karplus, P A.]]	+	[[Category: Karplus, P A]]
	[[Category: Flavodoxin-like fold]]		[[Category: Flavodoxin-like fold]]
	[[Category: Nadph-dependent fmn reductase]]		[[Category: Nadph-dependent fmn reductase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Ssud]]		[[Category: Ssud]]

OCA at 07:56, 18 June 2014

OCA — Wed, 18 Jun 2014 07:56:54 GMT

←Older revision		Revision as of 07:56, 18 June 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of the Escherichia coli alkanesulfonate FMN reductase SsuE in FMNH2-bound form==
		+	<StructureSection load='4pu0' size='340' side='right' caption='[[4pu0]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4pu0]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PU0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PU0 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pty\|4pty]], [[4ptz\|4ptz]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/FMN_reductase_(NADPH) FMN reductase (NADPH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.38 1.5.1.38] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pu0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pu0 RCSB], [http://www.ebi.ac.uk/pdbsum/4pu0 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Escherichia coli sulfur starvation utilization (ssu) operon includes a two-component monooxygenase system consisting of a nicotinamide adenine dinucleotide phosphate (NADPH)-dependent flavin mononucleotide (FMN) reductase, SsuE, and a monooxygenase, SsuD. SsuE is part of the flavodoxin-like superfamily, and we report here the crystal structures of its apo, FMN-bound, and FMNH2-bound forms at approximately 2 A resolution. In the crystals, SsuE forms a tetramer that is a dimer of dimers similar to those seen for homologous FMN reductases, quinone reductases, and the WrbA family of enzymes. A pi-helix present at the tetramer building interface is unique to the reductases from two-component monooxygenase systems. Analytical ultracentrifugation studies of SsuE confirm a dimer-tetramer equilibrium exists in solution, with FMN binding favoring the dimer. As the active site includes residues from both subunits, at least a dimeric association is required for the function of SsuE. The structures show that one FMN binds tightly in a deeply held site, which makes available a second binding site, in which either a second FMN or the nicotinamide of NADPH can bind. The FMNH2-bound structure shows subtle changes consistent with its binding being weaker than that of FMN. Combining this information with published kinetic studies, we propose a general catalytic cycle for two-component reductases of the flavodoxin-like superfamily, by which the enzyme can potentially provide FMNH2 to its partner monooxygenase by different routes depending on the FMN concentration and the presence of a partner monooxygenase.

-	~~The entry 4pu0 is ON HOLD until Paper Publication~~	+	Crystal Structure of Escherichia coli SsuE: Defining a General Catalytic Cycle for FMN Reductases of the Flavodoxin-like Superfamily.,Driggers CM, Dayal PV, Ellis HR, Karplus PA Biochemistry. 2014 Jun 3;53(21):3509-19. doi: 10.1021/bi500314f. Epub 2014 May, 21. PMID:24816272<ref>PMID:24816272</ref>

-	~~Authors~~: Driggers, C.M., Ellis, H.R., Karplus, P.A.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of the Escherichia coli alkanesulfonate FMN reductase SsuE in FMNH2~~-~~bound form~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Driggers, C M.]]
		+	[[Category: Ellis, H R.]]
		+	[[Category: Karplus, P A.]]
		+	[[Category: Flavodoxin-like fold]]
		+	[[Category: Nadph-dependent fmn reductase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Ssud]]

OCA at 07:46, 7 May 2014

OCA — Wed, 07 May 2014 07:46:30 GMT

←Older revision		Revision as of 07:46, 7 May 2014
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	Authors: Driggers, C.M., Ellis, H.R., Karplus, P.A.		Authors: Driggers, C.M., Ellis, H.R., Karplus, P.A.

-	Description: FMNH2-bound ~~SsuE~~	+	Description: Crystal structure of the Escherichia coli alkanesulfonate FMN reductase SsuE in FMNH2-bound form

OCA at 05:56, 9 April 2014

OCA — Wed, 09 Apr 2014 05:56:45 GMT

←Older revision		Revision as of 05:56, 9 April 2014
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4pu0 is ON HOLD	+	The entry 4pu0 is ON HOLD until Paper Publication

	Authors: Driggers, C.M., Ellis, H.R., Karplus, P.A.		Authors: Driggers, C.M., Ellis, H.R., Karplus, P.A.

	Description: FMNH2-bound SsuE		Description: FMNH2-bound SsuE

OCA: Protected "4pu0" [edit=sysop:move=sysop]

OCA — Wed, 19 Mar 2014 07:43:59 GMT

Protected "4pu0" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:43, 19 March 2014

OCA: New page: '''Unreleased structure''' The entry 4pu0 is ON HOLD Authors: Driggers, C.M., Ellis, H.R., Karplus, P.A. Description: FMNH2-bound SsuE

OCA — Wed, 19 Mar 2014 07:43:57 GMT

New page: '''Unreleased structure''' The entry 4pu0 is ON HOLD Authors: Driggers, C.M., Ellis, H.R., Karplus, P.A. Description: FMNH2-bound SsuE

New page

'''Unreleased structure'''

The entry 4pu0 is ON HOLD

Authors: Driggers, C.M., Ellis, H.R., Karplus, P.A.

Description: FMNH2-bound SsuE