4q2g - Revision history

OCA at 08:58, 20 March 2024

OCA — Wed, 20 Mar 2024 08:58:27 GMT

←Older revision		Revision as of 08:58, 20 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4q2g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q2G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q2G FirstGlance]. <br>		<table><tr><td colspan='2'>[[4q2g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q2G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q2G FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q2g OCA], [https://pdbe.org/4q2g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q2g RCSB], [https://www.ebi.ac.uk/pdbsum/4q2g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q2g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q2g OCA], [https://pdbe.org/4q2g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q2g RCSB], [https://www.ebi.ac.uk/pdbsum/4q2g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q2g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/CDSA_THEMA CDSA_THEMA]		[https://www.uniprot.org/uniprot/CDSA_THEMA CDSA_THEMA]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Phospholipids are elemental building-block molecules for biological membranes. Biosynthesis of phosphatidylinositol, phosphatidylglycerol and phosphatidylserine requires a central liponucleotide intermediate named cytidine-diphosphate diacylglycerol (CDP-DAG). The CDP-DAG synthetase (Cds) is an integral membrane enzyme catalysing the formation of CDP-DAG, an essential step for phosphoinositide recycling during signal transduction. Here we report the structure of the Cds from Thermotoga maritima (TmCdsA) at 3.4 A resolution. TmCdsA forms a homodimer and each monomer contains nine transmembrane helices arranged into a novel fold with three domains. An unusual funnel-shaped cavity penetrates half way into the membrane, allowing the enzyme to simultaneously accept hydrophilic substrate (cytidine 5'-triphosphate (CTP)/deoxy-CTP) from cytoplasm and hydrophobic substrate (phosphatidic acid) from membrane. Located at the bottom of the cavity, a Mg(2+)-K(+) hetero-di-metal centre coordinated by an Asp-Asp dyad serves as the cofactor of TmCdsA. The results suggest a two-metal-ion catalytic mechanism for the Cds-mediated synthesis of CDP-DAG at the membrane-cytoplasm interface.
-
-	Structure and mechanism of an intramembrane liponucleotide synthetase central for phospholipid biosynthesis.,Liu X, Yin Y, Wu J, Liu Z Nat Commun. 2014 Jun 27;5:4244. doi: 10.1038/ncomms5244. PMID:24968740<ref>PMID:24968740</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4q2g" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:41, 8 February 2023

OCA — Wed, 08 Feb 2023 07:41:09 GMT

←Older revision		Revision as of 07:41, 8 February 2023
Line 3:		Line 3:
	<StructureSection load='4q2g' size='340' side='right'caption='[[4q2g]], [[Resolution\|resolution]] 3.40Å' scene=''>		<StructureSection load='4q2g' size='340' side='right'caption='[[4q2g]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4q2g]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Thema Thema~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q2G OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4Q2G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4q2g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q2G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q2G FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q2e\|4q2e]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q2g OCA], [https://pdbe.org/4q2g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q2g RCSB], [https://www.ebi.ac.uk/pdbsum/4q2g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q2g ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cdsA, TM_1397 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidate_cytidylyltransferase Phosphatidate cytidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.41 2.7.7.41] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4q2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q2g OCA], [~~http~~://pdbe.org/4q2g PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4q2g RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4q2g PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4q2g ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CDSA_THEMA CDSA_THEMA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Phosphatidate cytidylyltransferase]]~~	+	[[Category: Thermotoga maritima MSB8]]
-	~~[[Category: Thema~~]]	+	[[Category: Liu X]]
-	[[Category: Liu, X]]	+	[[Category: Liu Z]]
-	[[Category: Liu, Z]]	+	[[Category: Wu J]]
-	[[Category: Wu, J]]	+	[[Category: Yin Y]]
-	[[Category: Yin, Y]]	+
-	~~[[Category: Cdsa fold]]~~	+
-	~~[[Category: Ctp and phosphatidic acid binding]]~~	+
-	~~[[Category: Intramembrane enzyme]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Nucleotidyltransferase]]~~	+
-	~~[[Category: Phospholipid biosynthesis lipid metabolism]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 09:02, 24 April 2019

OCA — Wed, 24 Apr 2019 09:02:57 GMT

←Older revision		Revision as of 09:02, 24 April 2019
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	==CRYSTAL STRUCTURE OF AN INTRAMEMBRANE CDP-DAG SYNTHETASE CENTRAL FOR PHOSPHOLIPID BIOSYNTHESIS (S200C/S223C, inactive mutant)==		==CRYSTAL STRUCTURE OF AN INTRAMEMBRANE CDP-DAG SYNTHETASE CENTRAL FOR PHOSPHOLIPID BIOSYNTHESIS (S200C/S223C, inactive mutant)==
-	<StructureSection load='4q2g' size='340' side='right' caption='[[4q2g]], [[Resolution\|resolution]] 3.40Å' scene=''>	+	<StructureSection load='4q2g' size='340' side='right'caption='[[4q2g]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4q2g]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q2G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q2G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4q2g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thema Thema]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q2G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q2G FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q2e\|4q2e]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q2e\|4q2e]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cdsA, TM_1397 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidate_cytidylyltransferase Phosphatidate cytidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.41 2.7.7.41] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidate_cytidylyltransferase Phosphatidate cytidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.41 2.7.7.41] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q2g OCA], [http://pdbe.org/4q2g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4q2g RCSB], [http://www.ebi.ac.uk/pdbsum/4q2g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4q2g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q2g OCA], [http://pdbe.org/4q2g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4q2g RCSB], [http://www.ebi.ac.uk/pdbsum/4q2g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4q2g ProSAT]</span></td></tr>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Phosphatidate cytidylyltransferase]]		[[Category: Phosphatidate cytidylyltransferase]]
		+	[[Category: Thema]]
	[[Category: Liu, X]]		[[Category: Liu, X]]
	[[Category: Liu, Z]]		[[Category: Liu, Z]]

OCA at 10:59, 11 August 2016

OCA — Thu, 11 Aug 2016 10:59:54 GMT

←Older revision		Revision as of 10:59, 11 August 2016
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF AN INTRAMEMBRANE CDP-DAG SYNTHETASE CENTRAL FOR PHOSPHOLIPID BIOSYNTHESIS (S200C/S223C, inactive mutant)==		==CRYSTAL STRUCTURE OF AN INTRAMEMBRANE CDP-DAG SYNTHETASE CENTRAL FOR PHOSPHOLIPID BIOSYNTHESIS (S200C/S223C, inactive mutant)==
	<StructureSection load='4q2g' size='340' side='right' caption='[[4q2g]], [[Resolution\|resolution]] 3.40Å' scene=''>		<StructureSection load='4q2g' size='340' side='right' caption='[[4q2g]], [[Resolution\|resolution]] 3.40Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q2e\|4q2e]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q2e\|4q2e]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidate_cytidylyltransferase Phosphatidate cytidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.41 2.7.7.41] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidate_cytidylyltransferase Phosphatidate cytidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.41 2.7.7.41] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q2g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4q2g RCSB], [http://www.ebi.ac.uk/pdbsum/4q2g PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q2g OCA], [http://pdbe.org/4q2g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4q2g RCSB], [http://www.ebi.ac.uk/pdbsum/4q2g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4q2g ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4q2g" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:43, 5 January 2015

OCA — Mon, 05 Jan 2015 12:43:49 GMT

←Older revision		Revision as of 12:43, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4q2g]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q2G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q2G FirstGlance]. <br>		<table><tr><td colspan='2'>[[4q2g]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q2G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q2G FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q2e\|4q2e]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q2e\|4q2e]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidate_cytidylyltransferase Phosphatidate cytidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.41 2.7.7.41] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidate_cytidylyltransferase Phosphatidate cytidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.41 2.7.7.41] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q2g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4q2g RCSB], [http://www.ebi.ac.uk/pdbsum/4q2g PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q2g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4q2g RCSB], [http://www.ebi.ac.uk/pdbsum/4q2g PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Phosphatidate cytidylyltransferase]]		[[Category: Phosphatidate cytidylyltransferase]]
-	[[Category: Liu, X.]]	+	[[Category: Liu, X]]
-	[[Category: Liu, Z.]]	+	[[Category: Liu, Z]]
-	[[Category: Wu, J.]]	+	[[Category: Wu, J]]
-	[[Category: Yin, Y.]]	+	[[Category: Yin, Y]]
	[[Category: Cdsa fold]]		[[Category: Cdsa fold]]
	[[Category: Ctp and phosphatidic acid binding]]		[[Category: Ctp and phosphatidic acid binding]]

OCA at 06:33, 30 July 2014

OCA — Wed, 30 Jul 2014 06:33:39 GMT

←Older revision		Revision as of 06:33, 30 July 2014
Line 8:		Line 8:
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q2g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4q2g RCSB], [http://www.ebi.ac.uk/pdbsum/4q2g PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q2g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4q2g RCSB], [http://www.ebi.ac.uk/pdbsum/4q2g PDBsum]</span></td></tr>
	<table>		<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Phospholipids are elemental building-block molecules for biological membranes. Biosynthesis of phosphatidylinositol, phosphatidylglycerol and phosphatidylserine requires a central liponucleotide intermediate named cytidine-diphosphate diacylglycerol (CDP-DAG). The CDP-DAG synthetase (Cds) is an integral membrane enzyme catalysing the formation of CDP-DAG, an essential step for phosphoinositide recycling during signal transduction. Here we report the structure of the Cds from Thermotoga maritima (TmCdsA) at 3.4 A resolution. TmCdsA forms a homodimer and each monomer contains nine transmembrane helices arranged into a novel fold with three domains. An unusual funnel-shaped cavity penetrates half way into the membrane, allowing the enzyme to simultaneously accept hydrophilic substrate (cytidine 5'-triphosphate (CTP)/deoxy-CTP) from cytoplasm and hydrophobic substrate (phosphatidic acid) from membrane. Located at the bottom of the cavity, a Mg(2+)-K(+) hetero-di-metal centre coordinated by an Asp-Asp dyad serves as the cofactor of TmCdsA. The results suggest a two-metal-ion catalytic mechanism for the Cds-mediated synthesis of CDP-DAG at the membrane-cytoplasm interface.
		+
		+	Structure and mechanism of an intramembrane liponucleotide synthetase central for phospholipid biosynthesis.,Liu X, Yin Y, Wu J, Liu Z Nat Commun. 2014 Jun 27;5:4244. doi: 10.1038/ncomms5244. PMID:24968740<ref>PMID:24968740</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:53, 2 July 2014

OCA — Wed, 02 Jul 2014 08:53:11 GMT

←Older revision		Revision as of 08:53, 2 July 2014
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-	'''~~Unreleased structure~~'''	+	==CRYSTAL STRUCTURE OF AN INTRAMEMBRANE CDP-DAG SYNTHETASE CENTRAL FOR PHOSPHOLIPID BIOSYNTHESIS (S200C/S223C, inactive mutant)==
-		+	<StructureSection load='4q2g' size='340' side='right' caption='[[4q2g]], [[Resolution\|resolution]] 3.40Å' scene=''>
-	~~The entry~~ 4q2g is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4q2g]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q2G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q2G FirstGlance]. <br>
-	~~Authors~~: ~~Liu~~, X.Y., ~~Yin~~, Y., ~~Wu, J~~.J., Liu, Z.F.	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
-		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q2e\|4q2e]]</td></tr>
-	~~Description~~: ~~Crystal structure of an intramembrane CDP-DAG synthetase central for phospholipid~~ biosynthesis	+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidate_cytidylyltransferase Phosphatidate cytidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.41 2.7.7.41] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q2g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4q2g RCSB], [http://www.ebi.ac.uk/pdbsum/4q2g PDBsum]</span></td></tr>
		+	<table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Phosphatidate cytidylyltransferase]]
		+	[[Category: Liu, X.]]
		+	[[Category: Liu, Z.]]
		+	[[Category: Wu, J.]]
		+	[[Category: Yin, Y.]]
		+	[[Category: Cdsa fold]]
		+	[[Category: Ctp and phosphatidic acid binding]]
		+	[[Category: Intramembrane enzyme]]
		+	[[Category: Membrane]]
		+	[[Category: Nucleotidyltransferase]]
		+	[[Category: Phospholipid biosynthesis lipid metabolism]]
		+	[[Category: Transferase]]

OCA at 06:39, 30 April 2014

OCA — Wed, 30 Apr 2014 06:39:01 GMT

←Older revision		Revision as of 06:39, 30 April 2014
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4q2g is ON HOLD	+	The entry 4q2g is ON HOLD until Paper Publication

	Authors: Liu, X.Y., Yin, Y., Wu, J.J., Liu, Z.F.		Authors: Liu, X.Y., Yin, Y., Wu, J.J., Liu, Z.F.

	Description: Crystal structure of an intramembrane CDP-DAG synthetase central for phospholipid biosynthesis		Description: Crystal structure of an intramembrane CDP-DAG synthetase central for phospholipid biosynthesis

OCA: Protected "4q2g" [edit=sysop:move=sysop]

OCA — Wed, 16 Apr 2014 09:11:10 GMT

Protected "4q2g" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:11, 16 April 2014

OCA: New page: '''Unreleased structure''' The entry 4q2g is ON HOLD Authors: Liu, X.Y., Yin, Y., Wu, J.J., Liu, Z.F. Description: Crystal structure of an intramembrane CDP-DAG synthetase central for ...

OCA — Wed, 16 Apr 2014 09:11:09 GMT

New page: '''Unreleased structure''' The entry 4q2g is ON HOLD Authors: Liu, X.Y., Yin, Y., Wu, J.J., Liu, Z.F. Description: Crystal structure of an intramembrane CDP-DAG synthetase central for ...

New page

'''Unreleased structure'''

The entry 4q2g is ON HOLD

Authors: Liu, X.Y., Yin, Y., Wu, J.J., Liu, Z.F.

Description: Crystal structure of an intramembrane CDP-DAG synthetase central for phospholipid biosynthesis