4q72 - Revision history

OCA at 17:28, 20 September 2023

OCA — Wed, 20 Sep 2023 17:28:50 GMT

←Older revision		Revision as of 17:28, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4q72]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bradyrhizobium_diazoefficiens_USDA_110 Bradyrhizobium diazoefficiens USDA 110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q72 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q72 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4q72]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bradyrhizobium_diazoefficiens_USDA_110 Bradyrhizobium diazoefficiens USDA 110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q72 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q72 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q72 OCA], [https://pdbe.org/4q72 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q72 RCSB], [https://www.ebi.ac.uk/pdbsum/4q72 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q72 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q72 OCA], [https://pdbe.org/4q72 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q72 RCSB], [https://www.ebi.ac.uk/pdbsum/4q72 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q72 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:46, 8 February 2023

OCA — Wed, 08 Feb 2023 07:46:03 GMT

←Older revision		Revision as of 07:46, 8 February 2023
Line 3:		Line 3:
	<StructureSection load='4q72' size='340' side='right'caption='[[4q72]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4q72' size='340' side='right'caption='[[4q72]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4q72]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bradu Bradu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q72 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4Q72 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4q72]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bradyrhizobium_diazoefficiens_USDA_110 Bradyrhizobium diazoefficiens USDA 110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q72 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q72 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q71\|4q71]], [[4q73\|4q73]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q72 OCA], [https://pdbe.org/4q72 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q72 RCSB], [https://www.ebi.ac.uk/pdbsum/4q72 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q72 ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BJ6T_21270, blr7261, putA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224911 BRADU])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4q72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q72 OCA], [~~http~~://pdbe.org/4q72 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4q72 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4q72 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4q72 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q89E26_BRADU Q89E26_BRADU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bradu~~]]	+	[[Category: Bradyrhizobium diazoefficiens USDA 110]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Luo, M]]	+	[[Category: Luo M]]
-	[[Category: Pemberton~~, T A~~]]	+	[[Category: Pemberton TA]]
-	[[Category: Tanner~~, J J]]~~	+	[[Category: Tanner JJ]]
-	~~[[Category: 1-pyrroline-5-carboxylate dehydrogenase]]~~	+
-	~~[[Category: Beta-alpha barrel]]~~	+
-	~~[[Category: Fad binding]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Proline dehydrogenase]]~~	+
-	~~[[Category: Proline utilization some]]~~	+
-	~~[[Category: Puta]]~~	+
-	~~[[Category: Rossmann fold~~]]	+

OCA at 09:32, 24 April 2019

OCA — Wed, 24 Apr 2019 09:32:41 GMT

←Older revision		Revision as of 09:32, 24 April 2019
Line 1:		Line 1:

	==Crystal Structure of Bradyrhizobium japonicum Proline Utilization A (PutA) Mutant D779Y==		==Crystal Structure of Bradyrhizobium japonicum Proline Utilization A (PutA) Mutant D779Y==
-	<StructureSection load='4q72' size='340' side='right' caption='[[4q72]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='4q72' size='340' side='right'caption='[[4q72]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4q72]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q72 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q72 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4q72]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bradu Bradu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q72 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q72 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q71\|4q71]], [[4q73\|4q73]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q71\|4q71]], [[4q73\|4q73]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BJ6T_21270, blr7261, putA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224911 BRADU])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q72 OCA], [http://pdbe.org/4q72 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4q72 RCSB], [http://www.ebi.ac.uk/pdbsum/4q72 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4q72 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q72 OCA], [http://pdbe.org/4q72 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4q72 RCSB], [http://www.ebi.ac.uk/pdbsum/4q72 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4q72 ProSAT]</span></td></tr>
	</table>		</table>
Line 24:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bradu]]
		+	[[Category: Large Structures]]
	[[Category: Luo, M]]		[[Category: Luo, M]]
	[[Category: Pemberton, T A]]		[[Category: Pemberton, T A]]

OCA at 21:16, 11 August 2016

OCA — Thu, 11 Aug 2016 21:16:46 GMT

←Older revision		Revision as of 21:16, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of Bradyrhizobium japonicum Proline Utilization A (PutA) Mutant D779Y==		==Crystal Structure of Bradyrhizobium japonicum Proline Utilization A (PutA) Mutant D779Y==
	<StructureSection load='4q72' size='340' side='right' caption='[[4q72]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4q72' size='340' side='right' caption='[[4q72]], [[Resolution\|resolution]] 2.30Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q71\|4q71]], [[4q73\|4q73]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q71\|4q71]], [[4q73\|4q73]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q72 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4q72 RCSB], [http://www.ebi.ac.uk/pdbsum/4q72 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q72 OCA], [http://pdbe.org/4q72 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4q72 RCSB], [http://www.ebi.ac.uk/pdbsum/4q72 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4q72 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4q72" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Proline utilization A\|Proline utilization A]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:46, 20 January 2015

OCA — Tue, 20 Jan 2015 11:46:24 GMT

←Older revision		Revision as of 11:46, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4q72]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q72 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q72 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4q72]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q72 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q72 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q71\|4q71]], [[4q73\|4q73]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q71\|4q71]], [[4q73\|4q73]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q72 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4q72 RCSB], [http://www.ebi.ac.uk/pdbsum/4q72 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q72 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4q72 RCSB], [http://www.ebi.ac.uk/pdbsum/4q72 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Luo, M.]]	+	[[Category: Luo, M]]
-	[[Category: Pemberton, T A.]]	+	[[Category: Pemberton, T A]]
-	[[Category: Tanner, J J.]]	+	[[Category: Tanner, J J]]
	[[Category: 1-pyrroline-5-carboxylate dehydrogenase]]		[[Category: 1-pyrroline-5-carboxylate dehydrogenase]]
	[[Category: Beta-alpha barrel]]		[[Category: Beta-alpha barrel]]

OCA at 02:25, 7 August 2014

OCA — Thu, 07 Aug 2014 02:25:00 GMT

←Older revision		Revision as of 02:25, 7 August 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal Structure of Bradyrhizobium japonicum Proline Utilization A (PutA) Mutant D779Y==
		+	<StructureSection load='4q72' size='340' side='right' caption='[[4q72]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4q72]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q72 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q72 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q71\|4q71]], [[4q73\|4q73]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q72 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4q72 RCSB], [http://www.ebi.ac.uk/pdbsum/4q72 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Proline utilization A from Bradyrhizobium japonicum (BjPutA) is a bifunctional flavoenzyme that catalyzes the oxidation of proline to glutamate using fused proline dehydrogenase (PRODH) and Delta1-pyrroline-5-carboxylate dehydrogenase (P5CDH) domains. Recent crystal structures and kinetic data suggest an intramolecular channel connects the two active sites, promoting substrate channeling of the intermediate Delta1-pyrroline-5-carboxylate/glutamate-gamma-semialdehyde (P5C/GSA). In this work, the structure of the channel was explored by inserting large side chain residues at four positions along the channel in BjPutA. Kinetic analysis of the different mutants revealed replacement of D779 with Tyr (D779Y) or Trp (D779W) significantly decreased the overall rate of the PRODH-P5CDH channeling reaction. X-ray crystal structures of D779Y and D779W revealed that the large side chains caused a constriction in the central section of the tunnel, thus likely impeding the travel of P5C/GSA in the channel. The D779Y and D779W mutants have PRODH activity similar to that of wild-type BjPutA but exhibit significantly lower P5CDH activity, suggesting that exogenous P5C/GSA enters the channel upstream of Asp779. Replacement of nearby Asp778 with Tyr (D778Y) did not impact BjPutA channeling activity. Consistent with the kinetic results, the X-ray crystal structure of D778Y shows that the main channel pathway is not impacted; however, an off-cavity pathway is closed off from the channel. These findings provide evidence that the off-cavity pathway is not essential for substrate channeling in BjPutA.

-	~~The entry 4q72 is ON HOLD until Paper Publication~~	+	Kinetic and Structural Characterization of Tunnel-Perturbing Mutants in Bradyrhizobium japonicum Proline Utilization A.,Arentson BW, Luo M, Pemberton TA, Tanner JJ, Becker DF Biochemistry. 2014 Jul 30. PMID:25046425<ref>PMID:25046425</ref>

-	~~Authors: Tanner~~, J.J., Pemberton, T.A., ~~Luo, M~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal Structure of Bradyrhizobium japonicum~~ Proline ~~Utilization A (PutA) Mutant D779Y~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Luo, M.]]
		+	[[Category: Pemberton, T A.]]
		+	[[Category: Tanner, J J.]]
		+	[[Category: 1-pyrroline-5-carboxylate dehydrogenase]]
		+	[[Category: Beta-alpha barrel]]
		+	[[Category: Fad binding]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Proline dehydrogenase]]
		+	[[Category: Proline utilization some]]
		+	[[Category: Puta]]
		+	[[Category: Rossmann fold]]

OCA at 08:14, 21 May 2014

OCA — Wed, 21 May 2014 08:14:27 GMT

←Older revision		Revision as of 08:14, 21 May 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4q72 is ON HOLD	+	The entry 4q72 is ON HOLD until Paper Publication

	Authors: Tanner, J.J., Pemberton, T.A., Luo, M.		Authors: Tanner, J.J., Pemberton, T.A., Luo, M.

	Description: Crystal Structure of Bradyrhizobium japonicum Proline Utilization A (PutA) Mutant D779Y		Description: Crystal Structure of Bradyrhizobium japonicum Proline Utilization A (PutA) Mutant D779Y

OCA: Protected "4q72" [edit=sysop:move=sysop]

OCA — Wed, 30 Apr 2014 06:45:43 GMT

Protected "4q72" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:45, 30 April 2014

OCA: New page: '''Unreleased structure''' The entry 4q72 is ON HOLD Authors: Tanner, J.J., Pemberton, T.A., Luo, M. Description: Crystal Structure of Bradyrhizobium japonicum Proline Utilization A (P...

OCA — Wed, 30 Apr 2014 06:45:41 GMT

New page: '''Unreleased structure''' The entry 4q72 is ON HOLD Authors: Tanner, J.J., Pemberton, T.A., Luo, M. Description: Crystal Structure of Bradyrhizobium japonicum Proline Utilization A (P...

New page

'''Unreleased structure'''

The entry 4q72 is ON HOLD

Authors: Tanner, J.J., Pemberton, T.A., Luo, M.

Description: Crystal Structure of Bradyrhizobium japonicum Proline Utilization A (PutA) Mutant D779Y