4q7m - Revision history

OCA at 12:44, 1 March 2024

OCA — Fri, 01 Mar 2024 12:44:35 GMT

←Older revision		Revision as of 12:44, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4q7m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q7M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q7M FirstGlance]. <br>		<table><tr><td colspan='2'>[[4q7m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q7M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q7M FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q7m OCA], [https://pdbe.org/4q7m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q7m RCSB], [https://www.ebi.ac.uk/pdbsum/4q7m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q7m ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q7m OCA], [https://pdbe.org/4q7m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q7m RCSB], [https://www.ebi.ac.uk/pdbsum/4q7m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q7m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/Y288_THEMA Y288_THEMA]		[https://www.uniprot.org/uniprot/Y288_THEMA Y288_THEMA]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	ABC exporters are ubiquitous multidomain transport proteins that couple ATP hydrolysis at a pair of nucleotide binding domains to substrate transport across the lipid bilayer mediated by two transmembrane domains. Recently, the crystal structure of the heterodimeric ABC exporter TM287/288 was determined. One of its asymmetric ATP binding sites is called the degenerate site; it binds nucleotides tightly but is impaired in terms of ATP hydrolysis. Here we report the crystal structures of both isolated motor domains of TM287/288. Unexpectedly, structural elements constituting the degenerate ATP binding site are disordered in these crystals and become structured only in the context of the full-length transporter. In addition, hydrogen bonding patterns of key residues, including those of the catalytically important Walker B and the switch loop motifs, are fundamentally different in the solitary NBDs compared to those in the intact transport protein. The structures reveal crucial interdomain contacts that need to be established for the proper assembly of the functional transporter complex.
-
-	A Transporter Motor Taken Apart: Flexibility in the Nucleotide Binding Domains of a Heterodimeric ABC Exporter.,Bukowska MA, Hohl M, Geertsma ER, Hurlimann LM, Grutter MG, Seeger MA Biochemistry. 2015 May 19;54(19):3086-99. doi: 10.1021/acs.biochem.5b00188. Epub , 2015 May 7. PMID:25947941<ref>PMID:25947941</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4q7m" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:47, 8 February 2023

OCA — Wed, 08 Feb 2023 07:47:16 GMT

←Older revision		Revision as of 07:47, 8 February 2023
Line 1:		Line 1:

	==Structure of NBD288-Avi of TM287/288==		==Structure of NBD288-Avi of TM287/288==
-	<StructureSection load='4q7m' size='340' side='right' caption='[[4q7m]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='4q7m' size='340' side='right'caption='[[4q7m]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4q7m]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Thema Thema~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q7M OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4Q7M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4q7m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q7M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q7M FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qf4\|3qf4]], [[4q7k\|4q7k]], [[4q7l\|4q7l]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q7m OCA], [https://pdbe.org/4q7m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q7m RCSB], [https://www.ebi.ac.uk/pdbsum/4q7m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q7m ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM_0288 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA])</td></tr>~~	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4q7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q7m OCA], [~~http~~://pdbe.org/4q7m PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4q7m RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4q7m PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4q7m ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Y288_THEMA Y288_THEMA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Thema~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Bukowska, M A~~]]	+	[[Category: Thermotoga maritima MSB8]]
-	[[Category: ~~Gruetter, M G~~]]	+	[[Category: Bukowska MA]]
-	[[Category: ~~Hohl, M~~]]	+	[[Category: Gruetter MG]]
-	[[Category: ~~Seeger,~~ M A]]	+	[[Category: Hohl M]]
-	[[Category: ~~Metal binding protein~~]]	+	[[Category: Seeger MA]]

OCA at 08:50, 22 November 2017

OCA — Wed, 22 Nov 2017 08:50:29 GMT

←Older revision		Revision as of 08:50, 22 November 2017
Line 3:		Line 3:
	<StructureSection load='4q7m' size='340' side='right' caption='[[4q7m]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4q7m' size='340' side='right' caption='[[4q7m]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4q7m]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q7M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q7M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4q7m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thema Thema]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q7M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q7M FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qf4\|3qf4]], [[4q7k\|4q7k]], [[4q7l\|4q7l]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qf4\|3qf4]], [[4q7k\|4q7k]], [[4q7l\|4q7l]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM_0288 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q7m OCA], [http://pdbe.org/4q7m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4q7m RCSB], [http://www.ebi.ac.uk/pdbsum/4q7m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4q7m ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q7m OCA], [http://pdbe.org/4q7m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4q7m RCSB], [http://www.ebi.ac.uk/pdbsum/4q7m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4q7m ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Thema]]
	[[Category: Bukowska, M A]]		[[Category: Bukowska, M A]]
	[[Category: Gruetter, M G]]		[[Category: Gruetter, M G]]

OCA at 17:35, 9 March 2017

OCA — Thu, 09 Mar 2017 17:35:56 GMT

←Older revision		Revision as of 17:35, 9 March 2017
Line 1:		Line 1:
		+
	==Structure of NBD288-Avi of TM287/288==		==Structure of NBD288-Avi of TM287/288==
	<StructureSection load='4q7m' size='340' side='right' caption='[[4q7m]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4q7m' size='340' side='right' caption='[[4q7m]], [[Resolution\|resolution]] 2.30Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4q7m]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q7M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q7M FirstGlance]. <br>		<table><tr><td colspan='2'>[[4q7m]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q7M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q7M FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qf4\|3qf4]], [[4q7k\|4q7k]], [[4q7l\|4q7l]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qf4\|3qf4]], [[4q7k\|4q7k]], [[4q7l\|4q7l]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q7m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4q7m RCSB], [http://www.ebi.ac.uk/pdbsum/4q7m PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q7m OCA], [http://pdbe.org/4q7m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4q7m RCSB], [http://www.ebi.ac.uk/pdbsum/4q7m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4q7m ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4q7m" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:10, 20 May 2015

OCA — Wed, 20 May 2015 12:10:57 GMT

←Older revision		Revision as of 12:10, 20 May 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Structure of NBD288-Avi of TM287/288==
		+	<StructureSection load='4q7m' size='340' side='right' caption='[[4q7m]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4q7m]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q7M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q7M FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qf4\|3qf4]], [[4q7k\|4q7k]], [[4q7l\|4q7l]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q7m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4q7m RCSB], [http://www.ebi.ac.uk/pdbsum/4q7m PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	ABC exporters are ubiquitous multidomain transport proteins that couple ATP hydrolysis at a pair of nucleotide binding domains to substrate transport across the lipid bilayer mediated by two transmembrane domains. Recently, the crystal structure of the heterodimeric ABC exporter TM287/288 was determined. One of its asymmetric ATP binding sites is called the degenerate site; it binds nucleotides tightly but is impaired in terms of ATP hydrolysis. Here we report the crystal structures of both isolated motor domains of TM287/288. Unexpectedly, structural elements constituting the degenerate ATP binding site are disordered in these crystals and become structured only in the context of the full-length transporter. In addition, hydrogen bonding patterns of key residues, including those of the catalytically important Walker B and the switch loop motifs, are fundamentally different in the solitary NBDs compared to those in the intact transport protein. The structures reveal crucial interdomain contacts that need to be established for the proper assembly of the functional transporter complex.

-	~~The entry 4q7m is ON HOLD until Jul 29 2016~~	+	A Transporter Motor Taken Apart: Flexibility in the Nucleotide Binding Domains of a Heterodimeric ABC Exporter.,Bukowska MA, Hohl M, Geertsma ER, Hurlimann LM, Grutter MG, Seeger MA Biochemistry. 2015 May 19;54(19):3086-99. doi: 10.1021/acs.biochem.5b00188. Epub , 2015 May 7. PMID:25947941<ref>PMID:25947941</ref>

-	~~Authors: Bukowska~~, M.A.~~, Hohl, M., Gruetter, M.G., Seeger, M.A~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Structure of NBD288-Avi of TM287~~/~~288~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
-	[[Category: Gruetter, M.G]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Bukowska, M A]]
		+	[[Category: Gruetter, M G]]
	[[Category: Hohl, M]]		[[Category: Hohl, M]]
-	[[Category: Seeger, M.A]]	+	[[Category: Seeger, M A]]
-	[[Category: ~~Bukowska, M.A~~]]	+	[[Category: Metal binding protein]]

OCA at 06:40, 8 April 2015

OCA — Wed, 08 Apr 2015 06:40:28 GMT

←Older revision		Revision as of 06:40, 8 April 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4q7m is ON HOLD until ~~Paper Publication~~	+	The entry 4q7m is ON HOLD until Jul 29 2016

	Authors: Bukowska, M.A., Hohl, M., Gruetter, M.G., Seeger, M.A.		Authors: Bukowska, M.A., Hohl, M., Gruetter, M.G., Seeger, M.A.

OCA at 15:28, 24 December 2014

OCA — Wed, 24 Dec 2014 15:28:09 GMT

←Older revision		Revision as of 15:28, 24 December 2014
Line 6:		Line 6:

	Description: Structure of NBD288-Avi of TM287/288		Description: Structure of NBD288-Avi of TM287/288
		+	[[Category: Unreleased Structures]]
		+	[[Category: Gruetter, M.G]]
		+	[[Category: Hohl, M]]
		+	[[Category: Seeger, M.A]]
		+	[[Category: Bukowska, M.A]]

OCA at 04:57, 4 June 2014

OCA — Wed, 04 Jun 2014 04:57:26 GMT

←Older revision		Revision as of 04:57, 4 June 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4q7m is ON HOLD	+	The entry 4q7m is ON HOLD until Paper Publication

	Authors: Bukowska, M.A., Hohl, M., Gruetter, M.G., Seeger, M.A.		Authors: Bukowska, M.A., Hohl, M., Gruetter, M.G., Seeger, M.A.

	Description: Structure of NBD288-Avi of TM287/288		Description: Structure of NBD288-Avi of TM287/288

OCA: Protected "4q7m" [edit=sysop:move=sysop]

OCA — Wed, 07 May 2014 07:49:46 GMT

Protected "4q7m" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:49, 7 May 2014

OCA: New page: '''Unreleased structure''' The entry 4q7m is ON HOLD Authors: Bukowska, M.A., Hohl, M., Gruetter, M.G., Seeger, M.A. Description: Structure of NBD288-Avi of TM287/288

OCA — Wed, 07 May 2014 07:49:45 GMT

New page: '''Unreleased structure''' The entry 4q7m is ON HOLD Authors: Bukowska, M.A., Hohl, M., Gruetter, M.G., Seeger, M.A. Description: Structure of NBD288-Avi of TM287/288

New page

'''Unreleased structure'''

The entry 4q7m is ON HOLD

Authors: Bukowska, M.A., Hohl, M., Gruetter, M.G., Seeger, M.A.

Description: Structure of NBD288-Avi of TM287/288