4ql7 - Revision history

OCA at 15:07, 8 November 2023

OCA — Wed, 08 Nov 2023 15:07:54 GMT

←Older revision		Revision as of 15:07, 8 November 2023
Line 1:		Line 1:

	==Crystal structure of C-terminus truncated Alkylhydroperoxide Reductase subunit C (AhpC1-172) from E. coli==		==Crystal structure of C-terminus truncated Alkylhydroperoxide Reductase subunit C (AhpC1-172) from E. coli==
-	<StructureSection load='4ql7' size='340' side='right'caption='[[4ql7]]' scene=''>	+	<StructureSection load='4ql7' size='340' side='right'caption='[[4ql7]], [[Resolution\|resolution]] 3.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QL7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QL7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ql7]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QL7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QL7 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ql7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ql7 OCA], [https://pdbe.org/4ql7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ql7 RCSB], [https://www.ebi.ac.uk/pdbsum/4ql7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ql7 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.75Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ql7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ql7 OCA], [https://pdbe.org/4ql7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ql7 RCSB], [https://www.ebi.ac.uk/pdbsum/4ql7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ql7 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	2-Cys peroxiredoxins (Prxs) are a large family of peroxidases, responsible for antioxidant function and regulation in cell signaling, apoptosis and differentiation. The Escherichia coli alkylhydroperoxide reductase (AhpR) is a prototype of the Prxs-family, and is composed of an NADH-dependent AhpF reductase (57kDa) and AhpC (21kDa), catalyzing the reduction of H2O2. We show that the E. coli AhpC (EcAhpC, 187 residues) forms a decameric ring structure under reduced and close to physiological conditions, composed of five catalytic dimers. Single particle analysis of cryo-electron micrographs of C-terminal truncated (EcAhpC1-172 and EcAhpC1-182) and mutated forms of EcAhpC reveals the loss of decamer formation, indicating the importance of the very C-terminus of AhpC in dimer to decamer transition. The crystallographic structures of the truncated EcAhpC1-172 and EcAhpC1-182 demonstrate for the first time that, in contrast to the reduced form, the very C-terminus of the oxidized EcAhpC is oriented away from the AhpC dimer interface and away from the catalytic redox-center, reflecting structural rearrangements during redox-modulation and -oligomerization. Furthermore, using an ensemble of different truncated and mutated EcAhpC protein constructs the importance of the very C-terminus in AhpC activity and in AhpC-AhpF assembly has been demonstrated.
		+
		+	Key roles of the Escherichia coli AhpC C-terminus in assembly and catalysis of alkylhydroperoxide reductase, an enzyme essential for the alleviation of oxidative stress.,Dip PV, Kamariah N, Nartey W, Beushausen C, Kostyuchenko VA, Ng TS, Lok SM, Saw WG, Eisenhaber F, Eisenhaber B, Gruber G Biochim Biophys Acta. 2014 Sep 2. pii: S0005-2728(14)00581-7. doi:, 10.1016/j.bbabio.2014.08.007. PMID:25193562<ref>PMID:25193562</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4ql7" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Eisenhaber B]]		[[Category: Eisenhaber B]]

OCA at 10:55, 31 August 2022

OCA — Wed, 31 Aug 2022 10:55:07 GMT

←Older revision		Revision as of 10:55, 31 August 2022
Line 1:		Line 1:

	==Crystal structure of C-terminus truncated Alkylhydroperoxide Reductase subunit C (AhpC1-172) from E. coli==		==Crystal structure of C-terminus truncated Alkylhydroperoxide Reductase subunit C (AhpC1-172) from E. coli==
-	<StructureSection load='4ql7' size='340' side='right'caption='[[4ql7]]~~, [[Resolution\|resolution]] 3.75Å~~' scene=''>	+	<StructureSection load='4ql7' size='340' side='right'caption='[[4ql7]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[4ql7]] is a 5 chain structure.~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QL7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QL7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QL7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QL7 FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4ql9\|4ql9]]</div></td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ql7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ql7 OCA], [https://pdbe.org/4ql7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ql7 RCSB], [https://www.ebi.ac.uk/pdbsum/4ql7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ql7 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ql7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ql7 OCA], [https://pdbe.org/4ql7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ql7 RCSB], [https://www.ebi.ac.uk/pdbsum/4ql7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ql7 ProSAT]</span></td></tr>	+
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	2-Cys peroxiredoxins (Prxs) are a large family of peroxidases, responsible for antioxidant function and regulation in cell signaling, apoptosis and differentiation. The Escherichia coli alkylhydroperoxide reductase (AhpR) is a prototype of the Prxs-family, and is composed of an NADH-dependent AhpF reductase (57kDa) and AhpC (21kDa), catalyzing the reduction of H2O2. We show that the E. coli AhpC (EcAhpC, 187 residues) forms a decameric ring structure under reduced and close to physiological conditions, composed of five catalytic dimers. Single particle analysis of cryo-electron micrographs of C-terminal truncated (EcAhpC1-172 and EcAhpC1-182) and mutated forms of EcAhpC reveals the loss of decamer formation, indicating the importance of the very C-terminus of AhpC in dimer to decamer transition. The crystallographic structures of the truncated EcAhpC1-172 and EcAhpC1-182 demonstrate for the first time that, in contrast to the reduced form, the very C-terminus of the oxidized EcAhpC is oriented away from the AhpC dimer interface and away from the catalytic redox-center, reflecting structural rearrangements during redox-modulation and -oligomerization. Furthermore, using an ensemble of different truncated and mutated EcAhpC protein constructs the importance of the very C-terminus in AhpC activity and in AhpC-AhpF assembly has been demonstrated.
-
-	Key roles of the Escherichia coli AhpC C-terminus in assembly and catalysis of alkylhydroperoxide reductase, an enzyme essential for the alleviation of oxidative stress.,Dip PV, Kamariah N, Nartey W, Beushausen C, Kostyuchenko VA, Ng TS, Lok SM, Saw WG, Eisenhaber F, Eisenhaber B, Gruber G Biochim Biophys Acta. 2014 Sep 2. pii: S0005-2728(14)00581-7. doi:, 10.1016/j.bbabio.2014.08.007. PMID:25193562<ref>PMID:25193562</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4ql7" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Eisenhaber, B]]	+	[[Category: Eisenhaber B]]
-	[[Category: Eisenhaber, F]]	+	[[Category: Eisenhaber F]]
-	[[Category: Gruber, G]]	+	[[Category: Gruber G]]
-	[[Category: Kamariah, N]]	+	[[Category: Kamariah N]]
-	~~[[Category: Ahpc]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Peroxiredoxin~~]]	+

OCA at 05:07, 25 August 2022

OCA — Thu, 25 Aug 2022 05:07:04 GMT

←Older revision		Revision as of 05:07, 25 August 2022
Line 3:		Line 3:
	<StructureSection load='4ql7' size='340' side='right'caption='[[4ql7]], [[Resolution\|resolution]] 3.75Å' scene=''>		<StructureSection load='4ql7' size='340' side='right'caption='[[4ql7]], [[Resolution\|resolution]] 3.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ql7]] is a 5 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QL7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4QL7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ql7]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QL7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QL7 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">~~[[4ql9\|4ql9]]~~<~~/td></tr>~~	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4ql9\|4ql9]]</div></td></tr>
-	~~<tr id~~='~~gene~~'~~><td class="sblockLbl"><b~~>[[~~Gene~~\|~~Gene:~~]]</b>~~</td><td class="sblockDat">ahpc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ql7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ql7 OCA], [https://pdbe.org/4ql7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ql7 RCSB], [https://www.ebi.ac.uk/pdbsum/4ql7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ql7 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ql7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ql7 OCA], [~~http~~://pdbe.org/4ql7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ql7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ql7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ql7 ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus coli migula 1895]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Eisenhaber, B]]		[[Category: Eisenhaber, B]]

OCA at 09:38, 24 April 2019

OCA — Wed, 24 Apr 2019 09:38:40 GMT

←Older revision		Revision as of 09:38, 24 April 2019
Line 1:		Line 1:

	==Crystal structure of C-terminus truncated Alkylhydroperoxide Reductase subunit C (AhpC1-172) from E. coli==		==Crystal structure of C-terminus truncated Alkylhydroperoxide Reductase subunit C (AhpC1-172) from E. coli==
-	<StructureSection load='4ql7' size='340' side='right' caption='[[4ql7]], [[Resolution\|resolution]] 3.75Å' scene=''>	+	<StructureSection load='4ql7' size='340' side='right'caption='[[4ql7]], [[Resolution\|resolution]] 3.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ql7]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QL7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QL7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ql7]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QL7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QL7 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ql9\|4ql9]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ql9\|4ql9]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ahpc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ql7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ql7 OCA], [http://pdbe.org/4ql7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ql7 RCSB], [http://www.ebi.ac.uk/pdbsum/4ql7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ql7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ql7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ql7 OCA], [http://pdbe.org/4ql7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ql7 RCSB], [http://www.ebi.ac.uk/pdbsum/4ql7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ql7 ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Eisenhaber, B]]		[[Category: Eisenhaber, B]]
	[[Category: Eisenhaber, F]]		[[Category: Eisenhaber, F]]

OCA at 12:19, 11 August 2016

OCA — Thu, 11 Aug 2016 12:19:19 GMT

←Older revision		Revision as of 12:19, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of C-terminus truncated Alkylhydroperoxide Reductase subunit C (AhpC1-172) from E. coli==		==Crystal structure of C-terminus truncated Alkylhydroperoxide Reductase subunit C (AhpC1-172) from E. coli==
	<StructureSection load='4ql7' size='340' side='right' caption='[[4ql7]], [[Resolution\|resolution]] 3.75Å' scene=''>		<StructureSection load='4ql7' size='340' side='right' caption='[[4ql7]], [[Resolution\|resolution]] 3.75Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4ql7]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QL7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QL7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ql7]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QL7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QL7 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ql9\|4ql9]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ql9\|4ql9]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ql7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ql7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ql7 RCSB], [http://www.ebi.ac.uk/pdbsum/4ql7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ql7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ql7 OCA], [http://pdbe.org/4ql7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ql7 RCSB], [http://www.ebi.ac.uk/pdbsum/4ql7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ql7 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4ql7" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:49, 20 January 2015

OCA — Tue, 20 Jan 2015 09:49:40 GMT

←Older revision		Revision as of 09:49, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ql7]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QL7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QL7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ql7]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QL7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QL7 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ql9\|4ql9]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ql9\|4ql9]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ql7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ql7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ql7 RCSB], [http://www.ebi.ac.uk/pdbsum/4ql7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ql7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ql7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ql7 RCSB], [http://www.ebi.ac.uk/pdbsum/4ql7 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 18:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Eisenhaber, B.]]	+	[[Category: Eisenhaber, B]]
-	[[Category: Eisenhaber, F.]]	+	[[Category: Eisenhaber, F]]
-	[[Category: Gruber, G.]]	+	[[Category: Gruber, G]]
-	[[Category: Kamariah, N.]]	+	[[Category: Kamariah, N]]
	[[Category: Ahpc]]		[[Category: Ahpc]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Peroxiredoxin]]		[[Category: Peroxiredoxin]]

OCA at 10:34, 24 September 2014

OCA — Wed, 24 Sep 2014 10:34:48 GMT

←Older revision		Revision as of 10:34, 24 September 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal structure of C-terminus truncated Alkylhydroperoxide Reductase subunit C (AhpC1-172) from E. coli==
		+	<StructureSection load='4ql7' size='340' side='right' caption='[[4ql7]], [[Resolution\|resolution]] 3.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4ql7]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QL7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QL7 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ql9\|4ql9]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ql7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ql7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ql7 RCSB], [http://www.ebi.ac.uk/pdbsum/4ql7 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	2-Cys peroxiredoxins (Prxs) are a large family of peroxidases, responsible for antioxidant function and regulation in cell signaling, apoptosis and differentiation. The Escherichia coli alkylhydroperoxide reductase (AhpR) is a prototype of the Prxs-family, and is composed of an NADH-dependent AhpF reductase (57kDa) and AhpC (21kDa), catalyzing the reduction of H2O2. We show that the E. coli AhpC (EcAhpC, 187 residues) forms a decameric ring structure under reduced and close to physiological conditions, composed of five catalytic dimers. Single particle analysis of cryo-electron micrographs of C-terminal truncated (EcAhpC1-172 and EcAhpC1-182) and mutated forms of EcAhpC reveals the loss of decamer formation, indicating the importance of the very C-terminus of AhpC in dimer to decamer transition. The crystallographic structures of the truncated EcAhpC1-172 and EcAhpC1-182 demonstrate for the first time that, in contrast to the reduced form, the very C-terminus of the oxidized EcAhpC is oriented away from the AhpC dimer interface and away from the catalytic redox-center, reflecting structural rearrangements during redox-modulation and -oligomerization. Furthermore, using an ensemble of different truncated and mutated EcAhpC protein constructs the importance of the very C-terminus in AhpC activity and in AhpC-AhpF assembly has been demonstrated.

-	~~The entry 4ql7 is ON HOLD until Paper Publication~~	+	Key roles of the Escherichia coli AhpC C-terminus in assembly and catalysis of alkylhydroperoxide reductase, an enzyme essential for the alleviation of oxidative stress.,Dip PV, Kamariah N, Nartey W, Beushausen C, Kostyuchenko VA, Ng TS, Lok SM, Saw WG, Eisenhaber F, Eisenhaber B, Gruber G Biochim Biophys Acta. 2014 Sep 2. pii: S0005-2728(14)00581-7. doi:, 10.1016/j.bbabio.2014.08.007. PMID:25193562<ref>PMID:25193562</ref>

-	~~Authors: Kamariah~~, N.~~, Gruber, G~~., Eisenhaber, F., ~~Eisenhaber~~, B.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of C-terminus truncated Alkylhydroperoxide Reductase subunit C (AhpC1-172) from E. coli~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Eisenhaber, B.]]
		+	[[Category: Eisenhaber, F.]]
		+	[[Category: Gruber, G.]]
		+	[[Category: Kamariah, N.]]
		+	[[Category: Ahpc]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Peroxiredoxin]]

OCA at 07:23, 2 July 2014

OCA — Wed, 02 Jul 2014 07:23:28 GMT

←Older revision		Revision as of 07:23, 2 July 2014
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4ql7 is ON HOLD	+	The entry 4ql7 is ON HOLD until Paper Publication

	Authors: Kamariah, N., Gruber, G., Eisenhaber, F., Eisenhaber, B.		Authors: Kamariah, N., Gruber, G., Eisenhaber, F., Eisenhaber, B.

	Description: Crystal structure of C-terminus truncated Alkylhydroperoxide Reductase subunit C (AhpC1-172) from E. coli		Description: Crystal structure of C-terminus truncated Alkylhydroperoxide Reductase subunit C (AhpC1-172) from E. coli

OCA: Protected "4ql7" [edit=sysop:move=sysop]

OCA — Wed, 18 Jun 2014 06:39:39 GMT

Protected "4ql7" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:39, 18 June 2014

OCA: New page: '''Unreleased structure''' The entry 4ql7 is ON HOLD Authors: Kamariah, N., Gruber, G., Eisenhaber, F., Eisenhaber, B. Description: Crystal structure of C-terminus truncated Alkylhydro...

OCA — Wed, 18 Jun 2014 06:39:37 GMT

New page: '''Unreleased structure''' The entry 4ql7 is ON HOLD Authors: Kamariah, N., Gruber, G., Eisenhaber, F., Eisenhaber, B. Description: Crystal structure of C-terminus truncated Alkylhydro...

New page

'''Unreleased structure'''

The entry 4ql7 is ON HOLD

Authors: Kamariah, N., Gruber, G., Eisenhaber, F., Eisenhaber, B.

Description: Crystal structure of C-terminus truncated Alkylhydroperoxide Reductase subunit C (AhpC1-172) from E. coli