4qll - Revision history

OCA at 15:08, 8 November 2023

OCA — Wed, 08 Nov 2023 15:08:29 GMT

←Older revision		Revision as of 15:08, 8 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4qll]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QLL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QLL FirstGlance]. <br>		<table><tr><td colspan='2'>[[4qll]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QLL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QLL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=PRD_900011:beta-cellotetraose'>PRD_900011</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=PRD_900011:beta-cellotetraose'>PRD_900011</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qll FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qll OCA], [https://pdbe.org/4qll PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qll RCSB], [https://www.ebi.ac.uk/pdbsum/4qll PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qll ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qll FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qll OCA], [https://pdbe.org/4qll PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qll RCSB], [https://www.ebi.ac.uk/pdbsum/4qll PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qll ProSAT]</span></td></tr>
	</table>		</table>

OCA at 11:34, 15 February 2023

OCA — Wed, 15 Feb 2023 11:34:47 GMT

←Older revision		Revision as of 11:34, 15 February 2023
Line 3:		Line 3:
	<StructureSection load='4qll' size='340' side='right'caption='[[4qll]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='4qll' size='340' side='right'caption='[[4qll]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4qll]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Japanese_rice Japanese rice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QLL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4QLL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4qll]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QLL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QLL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~CTT~~:BETA-D-~~GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE~~'>~~CTT~~</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=~~SO4~~:~~SULFATE+ION~~'>~~SO4~~</scene>, <scene name='pdbligand=ZN:~~ZINC~~+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=PRD_900011:beta-cellotetraose'>PRD_900011</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rgl\|2rgl]]~~, ~~[[3f5i\|3f5i]], [[3scn\|3scn]], [[3sct\|3sct]], [[3scw\|3scw]], [[4qlj\|4qlj]], [[4qlk\|4qlk]]~~<~~/td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qll FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qll OCA], [https://pdbe.org/4qll PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qll RCSB], [https://www.ebi.ac.uk/pdbsum/4qll PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qll ProSAT]</span></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:]]</b></td><td class="sblockDat">BGLU1, BGLU7, LOC_Os03g49600, Os03g0703000, Os3BGlu7, OSJNBa0004L11.16 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39947 Japanese rice])</td></tr>	+
-	~~<tr id='activity~~'>~~<td class="sblockLbl"><b>Activity:~~</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4qll FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qll OCA], [~~http~~://pdbe.org/4qll PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4qll RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4qll PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4qll ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/BGL07_ORYSJ BGL07_ORYSJ]] Hydrolyzes p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-mannoside, p-nitrophenyl beta-D-galactoside, p-nitrophenyl beta-D-xyloside, p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-arabinoside, oligosaccharides, pyridoxine beta-D-glucoside and the cyanogenic glucosides amygdalin, prunasin and dhurrin. Possesses pyridoxine transglucosylation activity.<ref>PMID:14692878</ref> <ref>PMID:19766588</ref> <ref>PMID:18308333</ref>	+	[https://www.uniprot.org/uniprot/BGL07_ORYSJ BGL07_ORYSJ] Hydrolyzes p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-mannoside, p-nitrophenyl beta-D-galactoside, p-nitrophenyl beta-D-xyloside, p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-arabinoside, oligosaccharides, pyridoxine beta-D-glucoside and the cyanogenic glucosides amygdalin, prunasin and dhurrin. Possesses pyridoxine transglucosylation activity.<ref>PMID:14692878</ref> <ref>PMID:19766588</ref> <ref>PMID:18308333</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Beta-glucosidase]]
-	[[Category: Japanese rice]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Cairns, J R.Ketudat~~]]	+	[[Category: Oryza sativa Japonica Group]]
-	[[Category: ~~Pengthaisong, S~~]]	+	[[Category: Ketudat Cairns JR]]
-	[[Category: ~~Beta-alpha-barrel]]~~	+	[[Category: Pengthaisong S]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Oligosaccharide binding]]~~	+
-	~~[[Category: Transglucosylation~~]]	+

OCA at 08:33, 24 April 2019

OCA — Wed, 24 Apr 2019 08:33:53 GMT

←Older revision		Revision as of 08:33, 24 April 2019
Line 1:		Line 1:

	==Crystal structure of rice BGlu1 E176Q/Y341A/Q187A mutant complexed with cellotetraose==		==Crystal structure of rice BGlu1 E176Q/Y341A/Q187A mutant complexed with cellotetraose==
-	<StructureSection load='4qll' size='340' side='right' caption='[[4qll]], [[Resolution\|resolution]] 1.85Å' scene=''>	+	<StructureSection load='4qll' size='340' side='right'caption='[[4qll]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4qll]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QLL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QLL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4qll]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Japanese_rice Japanese rice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QLL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QLL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rgl\|2rgl]], [[3f5i\|3f5i]], [[3scn\|3scn]], [[3sct\|3sct]], [[3scw\|3scw]], [[4qlj\|4qlj]], [[4qlk\|4qlk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rgl\|2rgl]], [[3f5i\|3f5i]], [[3scn\|3scn]], [[3sct\|3sct]], [[3scw\|3scw]], [[4qlj\|4qlj]], [[4qlk\|4qlk]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BGLU1, BGLU7, LOC_Os03g49600, Os03g0703000, Os3BGlu7, OSJNBa0004L11.16 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39947 Japanese rice])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qll FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qll OCA], [http://pdbe.org/4qll PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qll RCSB], [http://www.ebi.ac.uk/pdbsum/4qll PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qll ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qll FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qll OCA], [http://pdbe.org/4qll PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qll RCSB], [http://www.ebi.ac.uk/pdbsum/4qll PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qll ProSAT]</span></td></tr>
Line 22:		Line 23:

	==See Also==		==See Also==
-	*[[Beta-glucosidase\|Beta-glucosidase]]	+	*[[Beta-glucosidase 3D structures\|Beta-glucosidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Beta-glucosidase]]		[[Category: Beta-glucosidase]]
		+	[[Category: Japanese rice]]
		+	[[Category: Large Structures]]
	[[Category: Cairns, J R.Ketudat]]		[[Category: Cairns, J R.Ketudat]]
	[[Category: Pengthaisong, S]]		[[Category: Pengthaisong, S]]

OCA at 14:22, 29 March 2017

OCA — Wed, 29 Mar 2017 14:22:20 GMT

←Older revision		Revision as of 14:22, 29 March 2017
Line 1:		Line 1:
		+
	==Crystal structure of rice BGlu1 E176Q/Y341A/Q187A mutant complexed with cellotetraose==		==Crystal structure of rice BGlu1 E176Q/Y341A/Q187A mutant complexed with cellotetraose==
	<StructureSection load='4qll' size='340' side='right' caption='[[4qll]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='4qll' size='340' side='right' caption='[[4qll]], [[Resolution\|resolution]] 1.85Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rgl\|2rgl]], [[3f5i\|3f5i]], [[3scn\|3scn]], [[3sct\|3sct]], [[3scw\|3scw]], [[4qlj\|4qlj]], [[4qlk\|4qlk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rgl\|2rgl]], [[3f5i\|3f5i]], [[3scn\|3scn]], [[3sct\|3sct]], [[3scw\|3scw]], [[4qlj\|4qlj]], [[4qlk\|4qlk]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qll FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qll OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qll RCSB], [http://www.ebi.ac.uk/pdbsum/4qll PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qll FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qll OCA], [http://pdbe.org/4qll PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qll RCSB], [http://www.ebi.ac.uk/pdbsum/4qll PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qll ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4qll" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-glucosidase\|Beta-glucosidase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:51, 17 June 2015

OCA — Wed, 17 Jun 2015 14:51:47 GMT

←Older revision		Revision as of 14:51, 17 June 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of rice BGlu1 E176Q/Y341A/Q187A mutant complexed with cellotetraose==
		+	<StructureSection load='4qll' size='340' side='right' caption='[[4qll]], [[Resolution\|resolution]] 1.85Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4qll]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QLL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QLL FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rgl\|2rgl]], [[3f5i\|3f5i]], [[3scn\|3scn]], [[3sct\|3sct]], [[3scw\|3scw]], [[4qlj\|4qlj]], [[4qlk\|4qlk]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qll FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qll OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qll RCSB], [http://www.ebi.ac.uk/pdbsum/4qll PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BGL07_ORYSJ BGL07_ORYSJ]] Hydrolyzes p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-mannoside, p-nitrophenyl beta-D-galactoside, p-nitrophenyl beta-D-xyloside, p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-arabinoside, oligosaccharides, pyridoxine beta-D-glucoside and the cyanogenic glucosides amygdalin, prunasin and dhurrin. Possesses pyridoxine transglucosylation activity.<ref>PMID:14692878</ref> <ref>PMID:19766588</ref> <ref>PMID:18308333</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Rice BGlu1 (Os3BGlu7) is a glycoside hydrolase family 1 beta-glucosidase that hydrolyzes cellooligosaccharides with increasing efficiency as the degree of polymerization (DP) increases from 2 to 6, indicating six subsites for glucosyl residue binding. Five subsites have been identified in X-ray crystal structures of cellooligosaccharide complexes with its E176Q acid-base and E386G nucleophile mutants. X-ray crystal structures indicate that cellotetraose binds in a similar mode in BGlu1 E176Q and E386G, but in a different mode in the BGlu1 E386G/Y341A variant, in which glucosyl residue 4 (Glc4) interacts with Q187 instead of the eliminated phenolic group of Y341. Here, we found that the Q187A mutation has little effect on BGlu1 cellooligosaccharide hydrolysis activity or oligosaccharide binding in BGlu1 E176Q, and only slight effects on BGlu1 E386G glycosynthase activity. X-ray crystal structures showed that cellotetraose binds in a different position in BGlu1 E176Q/Y341A, in which it interacts directly with R178 and W337, and the Q187A mutation had little effect on cellotetraose binding. Mutations of R178 and W337 to A had significant and nonadditive effects on oligosaccharide hydrolysis by BGlu1, pNPGlc cleavage and cellooligosaccharide inhibition of BGlu1 E176Q and BGlu1 E386G glycosynthase activity. Hydrolysis activity was partially rescued by Y341 for longer substrates, suggesting stacking of Glc4 on Y341 stabilizes binding of cellooligosaccharides in the optimal position for hydrolysis. This analysis indicates that complex interactions between active site cleft residues modulate substrate binding and hydrolysis.

-	~~The entry 4qll is ON HOLD~~	+	Effects of active site cleft residues on oligosaccharide binding, hydrolysis, and glycosynthase activities of rice BGlu1 and its mutants.,Pengthaisong S, Ketudat Cairns JR Protein Sci. 2014 Dec;23(12):1738-52. doi: 10.1002/pro.2556. Epub 2014 Oct 23. PMID:25252199<ref>PMID:25252199</ref>

-	~~Authors: Pengthaisong~~, S.~~, Ketudat Cairns, J.R~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Crystal structure of rice BGlu1 E176Q~~/~~Y341A~~/~~Q187A mutant complexed with cellotetraose~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
-	[[Category: ~~Ketudat~~ Cairns, J.R]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Beta-glucosidase]]
		+	[[Category: Cairns, J R.Ketudat]]
	[[Category: Pengthaisong, S]]		[[Category: Pengthaisong, S]]
		+	[[Category: Beta-alpha-barrel]]
		+	[[Category: Hydrolase]]
		+	[[Category: Oligosaccharide binding]]
		+	[[Category: Transglucosylation]]

OCA at 15:54, 24 December 2014

OCA — Wed, 24 Dec 2014 15:54:38 GMT

←Older revision		Revision as of 15:54, 24 December 2014
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	Description: Crystal structure of rice BGlu1 E176Q/Y341A/Q187A mutant complexed with cellotetraose		Description: Crystal structure of rice BGlu1 E176Q/Y341A/Q187A mutant complexed with cellotetraose
		+	[[Category: Unreleased Structures]]
		+	[[Category: Ketudat Cairns, J.R]]
		+	[[Category: Pengthaisong, S]]

OCA at 05:56, 25 June 2014

OCA — Wed, 25 Jun 2014 05:56:15 GMT

←Older revision		Revision as of 05:56, 25 June 2014
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	The entry 4qll is ON HOLD		The entry 4qll is ON HOLD

-	Authors: Pengthaisong, S., Ketudat Cairns, J.R.~~, Center for Eukaryotic Structural Genomics (CESG)~~	+	Authors: Pengthaisong, S., Ketudat Cairns, J.R.

	Description: Crystal structure of rice BGlu1 E176Q/Y341A/Q187A mutant complexed with cellotetraose		Description: Crystal structure of rice BGlu1 E176Q/Y341A/Q187A mutant complexed with cellotetraose

OCA: Protected "4qll" [edit=sysop:move=sysop]

OCA — Wed, 18 Jun 2014 06:40:21 GMT

Protected "4qll" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:40, 18 June 2014

OCA: New page: '''Unreleased structure''' The entry 4qll is ON HOLD Authors: Pengthaisong, S., Ketudat Cairns, J.R., Center for Eukaryotic Structural Genomics (CESG) Description: Crystal structure of...

OCA — Wed, 18 Jun 2014 06:40:19 GMT

New page: '''Unreleased structure''' The entry 4qll is ON HOLD Authors: Pengthaisong, S., Ketudat Cairns, J.R., Center for Eukaryotic Structural Genomics (CESG) Description: Crystal structure of...

New page

'''Unreleased structure'''

The entry 4qll is ON HOLD

Authors: Pengthaisong, S., Ketudat Cairns, J.R., Center for Eukaryotic Structural Genomics (CESG)

Description: Crystal structure of rice BGlu1 E176Q/Y341A/Q187A mutant complexed with cellotetraose