4qlp - Revision history

OCA at 17:33, 20 September 2023

OCA — Wed, 20 Sep 2023 17:33:17 GMT

←Older revision		Revision as of 17:33, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4qlp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QLP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QLP FirstGlance]. <br>		<table><tr><td colspan='2'>[[4qlp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QLP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QLP FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qlp OCA], [https://pdbe.org/4qlp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qlp RCSB], [https://www.ebi.ac.uk/pdbsum/4qlp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qlp ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.1Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qlp OCA], [https://pdbe.org/4qlp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qlp RCSB], [https://www.ebi.ac.uk/pdbsum/4qlp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qlp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 11:34, 15 February 2023

OCA — Wed, 15 Feb 2023 11:34:56 GMT

←Older revision		Revision as of 11:34, 15 February 2023
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	<StructureSection load='4qlp' size='340' side='right'caption='[[4qlp]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='4qlp' size='340' side='right'caption='[[4qlp]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4qlp]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QLP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4QLP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4qlp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QLP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QLP FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HKBT1_4117, Rv3902c, Rv3903c, RVBD_3902c, RVBD_3903c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU]), HKBT1_4118, Rv3902c, Rv3903c, RVBD_3902c, RVBD_3903c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qlp OCA], [https://pdbe.org/4qlp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qlp RCSB], [https://www.ebi.ac.uk/pdbsum/4qlp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qlp ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4qlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qlp OCA], [~~http~~://pdbe.org/4qlp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4qlp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4qlp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4qlp ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/IFTNT_MYCTU IFTNT_MYCTU] Antitoxin for tuberculosis necrotizing toxin (TNT). Acts by binding directly to TNT, which inhibits NAD(+) glycohydrolase activity of TNT and protects M.tuberculosis from self-poisoning.<ref>PMID:26237511</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Myctu~~]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	[[Category: Cingolani, G]]	+	[[Category: Cingolani G]]
-	[[Category: Doornbos~~, K S~~]]	+	[[Category: Doornbos KS]]
-	[[Category: Lokareddy~~, R K~~]]	+	[[Category: Lokareddy RK]]
-	[[Category: Niederweis, M]]	+	[[Category: Niederweis M]]
-	[[Category: Siroy, A]]	+	[[Category: Siroy A]]
-	[[Category: Speer, A]]	+	[[Category: Speer A]]
-	[[Category: Sun, J]]	+	[[Category: Sun J]]
-	~~[[Category: B-nad+ glycohydrolase]]~~	+
-	~~[[Category: Duf4237]]~~	+
-	~~[[Category: Factor rv3902c renamed here as immunity factor of tnt]]~~	+
-	~~[[Category: Hydrolase-protein binding complex]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Nad-binding domain~~]]	+

OCA at 09:39, 24 April 2019

OCA — Wed, 24 Apr 2019 09:39:44 GMT

←Older revision		Revision as of 09:39, 24 April 2019
Line 1:		Line 1:
		+
	==Atomic structure of tuberculosis necrotizing toxin (TNT) complexed with its immunity factor IFT==		==Atomic structure of tuberculosis necrotizing toxin (TNT) complexed with its immunity factor IFT==
-	<StructureSection load='4qlp' size='340' side='right' caption='[[4qlp]], [[Resolution\|resolution]] 1.10Å' scene=''>	+	<StructureSection load='4qlp' size='340' side='right'caption='[[4qlp]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4qlp]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QLP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4qlp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QLP FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qlp OCA], [http://pdbe.org/4qlp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qlp RCSB], [http://www.ebi.ac.uk/pdbsum/4qlp PDBsum]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HKBT1_4117, Rv3902c, Rv3903c, RVBD_3902c, RVBD_3903c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU]), HKBT1_4118, Rv3902c, Rv3903c, RVBD_3902c, RVBD_3903c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qlp OCA], [http://pdbe.org/4qlp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qlp RCSB], [http://www.ebi.ac.uk/pdbsum/4qlp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qlp ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 18:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Myctu]]
	[[Category: Cingolani, G]]		[[Category: Cingolani, G]]
	[[Category: Doornbos, K S]]		[[Category: Doornbos, K S]]

OCA at 08:07, 30 September 2015

OCA — Wed, 30 Sep 2015 08:07:48 GMT

←Older revision		Revision as of 08:07, 30 September 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4qlp]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QLP FirstGlance]. <br>		<table><tr><td colspan='2'>[[4qlp]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QLP FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qlp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qlp RCSB], [http://www.ebi.ac.uk/pdbsum/4qlp PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qlp OCA], [http://pdbe.org/4qlp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qlp RCSB], [http://www.ebi.ac.uk/pdbsum/4qlp PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mycobacterium tuberculosis (Mtb) induces necrosis of infected cells to evade immune responses. Recently, we found that Mtb uses the protein CpnT to kill human macrophages by secreting its C-terminal domain, named tuberculosis necrotizing toxin (TNT), which induces necrosis by an unknown mechanism. Here we show that TNT gains access to the cytosol of Mtb-infected macrophages, where it hydrolyzes the essential coenzyme NAD(+). Expression or injection of a noncatalytic TNT mutant showed no cytotoxicity in macrophages or in zebrafish zygotes, respectively, thus demonstrating that the NAD(+) glycohydrolase activity is required for TNT-induced cell death. To prevent self-poisoning, Mtb produces an immunity factor for TNT (IFT) that binds TNT and inhibits its activity. The crystal structure of the TNT-IFT complex revealed a new NAD(+) glycohydrolase fold of TNT, the founding member of a toxin family widespread in pathogenic microorganisms.
		+
		+	The tuberculosis necrotizing toxin kills macrophages by hydrolyzing NAD.,Sun J, Siroy A, Lokareddy RK, Speer A, Doornbos KS, Cingolani G, Niederweis M Nat Struct Mol Biol. 2015 Sep;22(9):672-8. doi: 10.1038/nsmb.3064. Epub 2015 Aug , 3. PMID:26237511<ref>PMID:26237511</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4qlp" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 14:13, 22 July 2015

OCA — Wed, 22 Jul 2015 14:13:18 GMT

←Older revision		Revision as of 14:13, 22 July 2015
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-	~~'''Unreleased structure'''~~	+	==Atomic structure of tuberculosis necrotizing toxin (TNT) complexed with its immunity factor IFT==
-		+	<StructureSection load='4qlp' size='340' side='right' caption='[[4qlp]], [[Resolution\|resolution]] 1.10Å' scene=''>
-	~~The entry 4qlp is ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4qlp]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QLP FirstGlance]. <br>
-	~~Authors: Cingolani, G., Lokareddy, R.K., Sun, J., Siroy, A., Speer, A., Doornbos, K.S., Niederweis, M.~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qlp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qlp RCSB], [http://www.ebi.ac.uk/pdbsum/4qlp PDBsum]</span></td></tr>
-		+	</table>
-	~~Description:~~ Atomic structure of tuberculosis necrotizing toxin (TNT) complexed with its immunity factor IFT	+	__TOC__
-	[[~~Category: Unreleased Structures~~]]	+	</StructureSection>
-	[[~~Category: Sun, J~~]]	+
-	[[~~Category~~: ~~Lokareddy, R~~.K]]	+
-	[[~~Category~~: ~~Doornbos~~, K.S]]	+
	[[Category: Cingolani, G]]		[[Category: Cingolani, G]]
		+	[[Category: Doornbos, K S]]
		+	[[Category: Lokareddy, R K]]
	[[Category: Niederweis, M]]		[[Category: Niederweis, M]]
-	[[Category: Speer, A]]
	[[Category: Siroy, A]]		[[Category: Siroy, A]]
		+	[[Category: Speer, A]]
		+	[[Category: Sun, J]]
		+	[[Category: B-nad+ glycohydrolase]]
		+	[[Category: Duf4237]]
		+	[[Category: Factor rv3902c renamed here as immunity factor of tnt]]
		+	[[Category: Hydrolase-protein binding complex]]
		+	[[Category: Membrane]]
		+	[[Category: Nad-binding domain]]

OCA at 15:54, 24 December 2014

OCA — Wed, 24 Dec 2014 15:54:40 GMT

←Older revision		Revision as of 15:54, 24 December 2014
Line 6:		Line 6:

	Description: Atomic structure of tuberculosis necrotizing toxin (TNT) complexed with its immunity factor IFT		Description: Atomic structure of tuberculosis necrotizing toxin (TNT) complexed with its immunity factor IFT
		+	[[Category: Unreleased Structures]]
		+	[[Category: Sun, J]]
		+	[[Category: Lokareddy, R.K]]
		+	[[Category: Doornbos, K.S]]
		+	[[Category: Cingolani, G]]
		+	[[Category: Niederweis, M]]
		+	[[Category: Speer, A]]
		+	[[Category: Siroy, A]]

OCA at 07:09, 23 July 2014

OCA — Wed, 23 Jul 2014 07:09:59 GMT

←Older revision		Revision as of 07:09, 23 July 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4qlp is ON HOLD	+	The entry 4qlp is ON HOLD until Paper Publication

	Authors: Cingolani, G., Lokareddy, R.K., Sun, J., Siroy, A., Speer, A., Doornbos, K.S., Niederweis, M.		Authors: Cingolani, G., Lokareddy, R.K., Sun, J., Siroy, A., Speer, A., Doornbos, K.S., Niederweis, M.

	Description: Atomic structure of tuberculosis necrotizing toxin (TNT) complexed with its immunity factor IFT		Description: Atomic structure of tuberculosis necrotizing toxin (TNT) complexed with its immunity factor IFT

OCA: Protected "4qlp" [edit=sysop:move=sysop]

OCA — Wed, 18 Jun 2014 06:40:34 GMT

Protected "4qlp" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:40, 18 June 2014

OCA: New page: '''Unreleased structure''' The entry 4qlp is ON HOLD Authors: Cingolani, G., Lokareddy, R.K., Sun, J., Siroy, A., Speer, A., Doornbos, K.S., Niederweis, M. Description: Atomic structur...

OCA — Wed, 18 Jun 2014 06:40:33 GMT

New page: '''Unreleased structure''' The entry 4qlp is ON HOLD Authors: Cingolani, G., Lokareddy, R.K., Sun, J., Siroy, A., Speer, A., Doornbos, K.S., Niederweis, M. Description: Atomic structur...

New page

'''Unreleased structure'''

The entry 4qlp is ON HOLD

Authors: Cingolani, G., Lokareddy, R.K., Sun, J., Siroy, A., Speer, A., Doornbos, K.S., Niederweis, M.

Description: Atomic structure of tuberculosis necrotizing toxin (TNT) complexed with its immunity factor IFT