4qnl - Revision history

OCA at 13:42, 22 February 2023

OCA — Wed, 22 Feb 2023 13:42:14 GMT

←Older revision		Revision as of 13:42, 22 February 2023
Line 1:		Line 1:

	==Crystal structure of tail fiber protein gp63.1 from E. coli phage G7C==		==Crystal structure of tail fiber protein gp63.1 from E. coli phage G7C==
-	<StructureSection load='4qnl' size='340' side='right' caption='[[4qnl]], [[Resolution\|resolution]] 2.41Å' scene=''>	+	<StructureSection load='4qnl' size='340' side='right'caption='[[4qnl]], [[Resolution\|resolution]] 2.41Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4qnl]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Escherichia_coli_bacteriophage_vb_ecop_g7c~~ Escherichia ~~coli bacteriophage vb_ecop_g7c~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QNL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4QNL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4qnl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_vB_EcoP_G7C Escherichia phage vB_EcoP_G7C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QNL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QNL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qnl OCA], [https://pdbe.org/4qnl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qnl RCSB], [https://www.ebi.ac.uk/pdbsum/4qnl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qnl ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">63.1, gp63.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1054461 Escherichia coli bacteriophage vB_EcoP_G7C])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4qnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qnl OCA], [~~http~~://pdbe.org/4qnl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4qnl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4qnl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4qnl ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G0XNW5_9CAUD G0XNW5_9CAUD]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 4qnl" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4qnl" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Tailspike protein\|Tailspike protein]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Escherichia ~~coli bacteriophage vb_ecop_g7c~~]]	+	[[Category: Escherichia phage vB_EcoP_G7C]]
-	[[Category: Browning, C]]	+	[[Category: Large Structures]]
-	[[Category: Leiman~~, P G~~]]	+	[[Category: Browning C]]
-	[[Category: Letarov, A]]	+	[[Category: Leiman PG]]
-	[[Category: Prokhorov, N]]	+	[[Category: Letarov A]]
-	[[Category: Riccio, C]]	+	[[Category: Prokhorov N]]
-	~~[[Category: Adsorption of the phage on bacterial host]]~~	+	[[Category: Riccio C]]
-	~~[[Category: Bacterial lps digestion]]~~	+
-	~~[[Category: Distal end of the baseplate]]~~	+
-	~~[[Category: G7c phage]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hydrolase-type esterase]]~~	+
-	~~[[Category: Selenomethionine derivative]]~~	+
-	~~[[Category: Tail fiber]]~~	+
-	~~[[Category: Tail fiber protein gp66~~]]	+

OCA at 06:34, 20 December 2017

OCA — Wed, 20 Dec 2017 06:34:53 GMT

←Older revision		Revision as of 06:34, 20 December 2017
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qnl OCA], [http://pdbe.org/4qnl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qnl RCSB], [http://www.ebi.ac.uk/pdbsum/4qnl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qnl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qnl OCA], [http://pdbe.org/4qnl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qnl RCSB], [http://www.ebi.ac.uk/pdbsum/4qnl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qnl ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacteriophages recognize and bind to their hosts with the help of receptor-binding proteins (RBPs) that emanate from the phage particle in the form of fibers or tailspikes. RBPs show a great variability in their shapes, sizes, and location on the particle. Some RBPs are known to depolymerize surface polysaccharides of the host while others show no enzymatic activity. Here we report that both RBPs of podovirus G7C - tailspikes gp63.1 and gp66 - are essential for infection of its natural host bacterium E. coli 4s that populates the equine intestinal tract. We characterize the structure and function of gp63.1 and show that unlike any previously described RPB, gp63.1 deacetylates surface polysaccharides of E. coli 4s leaving the backbone of the polysaccharide intact. We demonstrate that gp63.1 and gp66 form a stable complex, in which the N-terminal part of gp66 serves as an attachment site for gp63.1 and anchors the gp63.1-gp66 complex to the G7C tail. The esterase domain of gp63.1 as well as domains mediating the gp63.1-gp66 interaction is widespread among all three families of tailed bacteriophages.
		+
		+	Function of bacteriophage G7C esterase tailspike in host cell adsorption.,Prokhorov NS, Riccio C, Zdorovenko EL, Shneider MM, Browning C, Knirel YA, Leiman PG, Letarov AV Mol Microbiol. 2017 Aug;105(3):385-398. doi: 10.1111/mmi.13710. Epub 2017 Jun 19. PMID:28513100<ref>PMID:28513100</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4qnl" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:55, 22 November 2017

OCA — Wed, 22 Nov 2017 08:55:57 GMT

←Older revision		Revision as of 08:55, 22 November 2017
Line 1:		Line 1:
		+
	==Crystal structure of tail fiber protein gp63.1 from E. coli phage G7C==		==Crystal structure of tail fiber protein gp63.1 from E. coli phage G7C==
	<StructureSection load='4qnl' size='340' side='right' caption='[[4qnl]], [[Resolution\|resolution]] 2.41Å' scene=''>		<StructureSection load='4qnl' size='340' side='right' caption='[[4qnl]], [[Resolution\|resolution]] 2.41Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4qnl]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QNL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QNL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4qnl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_bacteriophage_vb_ecop_g7c Escherichia coli bacteriophage vb_ecop_g7c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QNL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QNL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qnl OCA], [http://pdbe.org/4qnl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qnl RCSB], [http://www.ebi.ac.uk/pdbsum/4qnl PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">63.1, gp63.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1054461 Escherichia coli bacteriophage vB_EcoP_G7C])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qnl OCA], [http://pdbe.org/4qnl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qnl RCSB], [http://www.ebi.ac.uk/pdbsum/4qnl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qnl ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli bacteriophage vb_ecop_g7c]]
	[[Category: Browning, C]]		[[Category: Browning, C]]
	[[Category: Leiman, P G]]		[[Category: Leiman, P G]]

OCA at 20:51, 30 November 2015

OCA — Mon, 30 Nov 2015 20:51:31 GMT

←Older revision		Revision as of 20:51, 30 November 2015
Line 5:		Line 5:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qnl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qnl RCSB], [http://www.ebi.ac.uk/pdbsum/4qnl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qnl OCA], [http://pdbe.org/4qnl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qnl RCSB], [http://www.ebi.ac.uk/pdbsum/4qnl PDBsum]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 12:58, 24 June 2015

OCA — Wed, 24 Jun 2015 12:58:29 GMT

←Older revision		Revision as of 12:58, 24 June 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal structure of tail fiber protein gp63.1 from E. coli phage G7C==
-		+	<StructureSection load='4qnl' size='340' side='right' caption='[[4qnl]], [[Resolution\|resolution]] 2.41Å' scene=''>
-	~~The entry~~ 4qnl is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4qnl]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QNL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QNL FirstGlance]. <br>
-	~~Authors~~: ~~Riccio, C~~.~~, Browning, C~~.~~, Leiman, P~~.G.~~, Prokhorov, N~~.~~, Letarov, A~~.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~Description~~: ~~Crystal structure of tail fiber protein gp63.~~1 ~~from E. coli phage G7C~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qnl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qnl RCSB], [http://www.ebi.ac.uk/pdbsum/4qnl PDBsum]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Riccio~~, C]]	+	__TOC__
		+	</StructureSection>
	[[Category: Browning, C]]		[[Category: Browning, C]]
-	[[Category: ~~Prokhorov~~, N]]	+	[[Category: Leiman, P G]]
	[[Category: Letarov, A]]		[[Category: Letarov, A]]
-	[[Category: ~~Leiman~~, ~~P.G~~]]	+	[[Category: Prokhorov, N]]
		+	[[Category: Riccio, C]]
		+	[[Category: Adsorption of the phage on bacterial host]]
		+	[[Category: Bacterial lps digestion]]
		+	[[Category: Distal end of the baseplate]]
		+	[[Category: G7c phage]]
		+	[[Category: Hydrolase]]
		+	[[Category: Hydrolase-type esterase]]
		+	[[Category: Selenomethionine derivative]]
		+	[[Category: Tail fiber]]
		+	[[Category: Tail fiber protein gp66]]

OCA at 15:57, 24 December 2014

OCA — Wed, 24 Dec 2014 15:57:15 GMT

←Older revision		Revision as of 15:57, 24 December 2014
Line 6:		Line 6:

	Description: Crystal structure of tail fiber protein gp63.1 from E. coli phage G7C		Description: Crystal structure of tail fiber protein gp63.1 from E. coli phage G7C
		+	[[Category: Unreleased Structures]]
		+	[[Category: Riccio, C]]
		+	[[Category: Browning, C]]
		+	[[Category: Prokhorov, N]]
		+	[[Category: Letarov, A]]
		+	[[Category: Leiman, P.G]]

OCA at 04:05, 4 September 2014

OCA — Thu, 04 Sep 2014 04:05:44 GMT

←Older revision		Revision as of 04:05, 4 September 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4qnl is ON HOLD	+	The entry 4qnl is ON HOLD until Paper Publication

	Authors: Riccio, C., Browning, C., Leiman, P.G., Prokhorov, N., Letarov, A.		Authors: Riccio, C., Browning, C., Leiman, P.G., Prokhorov, N., Letarov, A.

	Description: Crystal structure of tail fiber protein gp63.1 from E. coli phage G7C		Description: Crystal structure of tail fiber protein gp63.1 from E. coli phage G7C

OCA: Protected "4qnl" [edit=sysop:move=sysop]

OCA — Wed, 25 Jun 2014 05:58:33 GMT

Protected "4qnl" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:58, 25 June 2014

OCA: New page: '''Unreleased structure''' The entry 4qnl is ON HOLD Authors: Riccio, C., Browning, C., Leiman, P.G., Prokhorov, N., Letarov, A. Description: Crystal structure of tail fiber protein gp...

OCA — Wed, 25 Jun 2014 05:58:32 GMT

New page: '''Unreleased structure''' The entry 4qnl is ON HOLD Authors: Riccio, C., Browning, C., Leiman, P.G., Prokhorov, N., Letarov, A. Description: Crystal structure of tail fiber protein gp...

New page

'''Unreleased structure'''

The entry 4qnl is ON HOLD

Authors: Riccio, C., Browning, C., Leiman, P.G., Prokhorov, N., Letarov, A.

Description: Crystal structure of tail fiber protein gp63.1 from E. coli phage G7C