4qnr - Revision history

OCA at 17:34, 20 September 2023

OCA — Wed, 20 Sep 2023 17:34:01 GMT

←Older revision		Revision as of 17:34, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4qnr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QNR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QNR FirstGlance]. <br>		<table><tr><td colspan='2'>[[4qnr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QNR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QNR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.539Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qnr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qnr OCA], [https://pdbe.org/4qnr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qnr RCSB], [https://www.ebi.ac.uk/pdbsum/4qnr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qnr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qnr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qnr OCA], [https://pdbe.org/4qnr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qnr RCSB], [https://www.ebi.ac.uk/pdbsum/4qnr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qnr ProSAT]</span></td></tr>
	</table>		</table>

OCA at 13:42, 22 February 2023

OCA — Wed, 22 Feb 2023 13:42:43 GMT

←Older revision		Revision as of 13:42, 22 February 2023
Line 3:		Line 3:
	<StructureSection load='4qnr' size='340' side='right'caption='[[4qnr]], [[Resolution\|resolution]] 1.54Å' scene=''>		<StructureSection load='4qnr' size='340' side='right'caption='[[4qnr]], [[Resolution\|resolution]] 1.54Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4qnr]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QNR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4QNR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4qnr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QNR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QNR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bjw\|2bjw]], [[2bjv\|2bjv]], [[2c98\|2c98]], [[2c96\|2c96]], [[2c99\|2c99]], [[2c9c\|2c9c]], [[4qnm\|4qnm]], [[4qos\|4qos]]</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qnr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qnr OCA], [https://pdbe.org/4qnr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qnr RCSB], [https://www.ebi.ac.uk/pdbsum/4qnr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qnr ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1303, JW1296, pspF, ycjB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4qnr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qnr OCA], [~~http~~://pdbe.org/4qnr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4qnr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4qnr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4qnr ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/PSPF_ECOLI PSPF_ECOLI]] Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene.<ref>PMID:8606168</ref> <ref>PMID:15485810</ref> <ref>PMID:19804784</ref>	+	[https://www.uniprot.org/uniprot/PSPF_ECOLI PSPF_ECOLI] Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene.<ref>PMID:8606168</ref> <ref>PMID:15485810</ref> <ref>PMID:19804784</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 20:

	==See Also==		==See Also==
-	*[[Transcriptional activator\|Transcriptional activator]]	+	*[[Transcriptional activator 3D structures\|Transcriptional activator 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Buck, M]]	+	[[Category: Buck M]]
-	[[Category: Burrows~~, P C~~]]	+	[[Category: Burrows PC]]
-	[[Category: Darbari~~, V C~~]]	+	[[Category: Darbari VC]]
-	[[Category: Joly, N]]	+	[[Category: Joly N]]
-	[[Category: Lawton, E]]	+	[[Category: Lawton E]]
-	[[Category: Lu, D]]	+	[[Category: Lu D]]
-	[[Category: Wiesler, S]]	+	[[Category: Wiesler S]]
-	[[Category: Zhang, N]]	+	[[Category: Zhang N]]
-	[[Category: Zhang, X]]	+	[[Category: Zhang X]]
-	~~[[Category: Aaa domain]]~~	+
-	~~[[Category: Atp binding]]~~	+
-	~~[[Category: Bacterial sigma54 activator]]~~	+
-	~~[[Category: Dna binding]]~~	+
-	~~[[Category: Transcription~~]]	+

OCA at 09:06, 24 April 2019

OCA — Wed, 24 Apr 2019 09:06:52 GMT

←Older revision		Revision as of 09:06, 24 April 2019
Line 1:		Line 1:

	==CRYSTAL STRUCTURE OF PSPF(1-265) E108Q MUTANT bound to ATP==		==CRYSTAL STRUCTURE OF PSPF(1-265) E108Q MUTANT bound to ATP==
-	<StructureSection load='4qnr' size='340' side='right' caption='[[4qnr]], [[Resolution\|resolution]] 1.54Å' scene=''>	+	<StructureSection load='4qnr' size='340' side='right'caption='[[4qnr]], [[Resolution\|resolution]] 1.54Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4qnr]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QNR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QNR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4qnr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QNR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QNR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bjw\|2bjw]], [[2bjv\|2bjv]], [[2c98\|2c98]], [[2c96\|2c96]], [[2c99\|2c99]], [[2c9c\|2c9c]], [[4qnm\|4qnm]], [[4qos\|4qos]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bjw\|2bjw]], [[2bjv\|2bjv]], [[2c98\|2c98]], [[2c96\|2c96]], [[2c99\|2c99]], [[2c9c\|2c9c]], [[4qnm\|4qnm]], [[4qos\|4qos]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1303, JW1296, pspF, ycjB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qnr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qnr OCA], [http://pdbe.org/4qnr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qnr RCSB], [http://www.ebi.ac.uk/pdbsum/4qnr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qnr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qnr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qnr OCA], [http://pdbe.org/4qnr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qnr RCSB], [http://www.ebi.ac.uk/pdbsum/4qnr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qnr ProSAT]</span></td></tr>
	</table>		</table>
Line 26:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Buck, M]]		[[Category: Buck, M]]
	[[Category: Burrows, P C]]		[[Category: Burrows, P C]]

OCA at 17:21, 4 August 2016

OCA — Thu, 04 Aug 2016 17:21:39 GMT

←Older revision		Revision as of 17:21, 4 August 2016
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF PSPF(1-265) E108Q MUTANT bound to ATP==		==CRYSTAL STRUCTURE OF PSPF(1-265) E108Q MUTANT bound to ATP==
	<StructureSection load='4qnr' size='340' side='right' caption='[[4qnr]], [[Resolution\|resolution]] 1.54Å' scene=''>		<StructureSection load='4qnr' size='340' side='right' caption='[[4qnr]], [[Resolution\|resolution]] 1.54Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bjw\|2bjw]], [[2bjv\|2bjv]], [[2c98\|2c98]], [[2c96\|2c96]], [[2c99\|2c99]], [[2c9c\|2c9c]], [[4qnm\|4qnm]], [[4qos\|4qos]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bjw\|2bjw]], [[2bjv\|2bjv]], [[2c98\|2c98]], [[2c96\|2c96]], [[2c99\|2c99]], [[2c9c\|2c9c]], [[4qnm\|4qnm]], [[4qos\|4qos]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qnr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qnr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qnr RCSB], [http://www.ebi.ac.uk/pdbsum/4qnr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qnr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qnr OCA], [http://pdbe.org/4qnr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qnr RCSB], [http://www.ebi.ac.uk/pdbsum/4qnr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qnr ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4qnr" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Transcriptional activator\|Transcriptional activator]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:30, 24 December 2014

OCA — Wed, 24 Dec 2014 17:30:54 GMT

←Older revision		Revision as of 17:30, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4qnr]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QNR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QNR FirstGlance]. <br>		<table><tr><td colspan='2'>[[4qnr]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QNR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QNR FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bjw\|2bjw]], [[2bjv\|2bjv]], [[2c98\|2c98]], [[2c96\|2c96]], [[2c99\|2c99]], [[2c9c\|2c9c]], [[4qnm\|4qnm]], [[4qos\|4qos]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bjw\|2bjw]], [[2bjv\|2bjv]], [[2c98\|2c98]], [[2c96\|2c96]], [[2c99\|2c99]], [[2c9c\|2c9c]], [[4qnm\|4qnm]], [[4qos\|4qos]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qnr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qnr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qnr RCSB], [http://www.ebi.ac.uk/pdbsum/4qnr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qnr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qnr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qnr RCSB], [http://www.ebi.ac.uk/pdbsum/4qnr PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PSPF_ECOLI PSPF_ECOLI]] Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene.<ref>PMID:8606168</ref> <ref>PMID:15485810</ref> <ref>PMID:19804784</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Buck, M.]]	+	[[Category: Buck, M]]
-	[[Category: Burrows, P C.]]	+	[[Category: Burrows, P C]]
-	[[Category: Darbari, V C.]]	+	[[Category: Darbari, V C]]
-	[[Category: Joly, N.]]	+	[[Category: Joly, N]]
-	[[Category: Lawton, E.]]	+	[[Category: Lawton, E]]
-	[[Category: Lu, D.]]	+	[[Category: Lu, D]]
-	[[Category: Wiesler, S.]]	+	[[Category: Wiesler, S]]
-	[[Category: Zhang, N.]]	+	[[Category: Zhang, N]]
-	[[Category: Zhang, X.]]	+	[[Category: Zhang, X]]
	[[Category: Aaa domain]]		[[Category: Aaa domain]]
	[[Category: Atp binding]]		[[Category: Atp binding]]

OCA at 02:30, 7 August 2014

OCA — Thu, 07 Aug 2014 02:30:26 GMT

←Older revision		Revision as of 02:30, 7 August 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==CRYSTAL STRUCTURE OF PSPF(1-265) E108Q MUTANT bound to ATP==
		+	<StructureSection load='4qnr' size='340' side='right' caption='[[4qnr]], [[Resolution\|resolution]] 1.54Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4qnr]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QNR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QNR FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bjw\|2bjw]], [[2bjv\|2bjv]], [[2c98\|2c98]], [[2c96\|2c96]], [[2c99\|2c99]], [[2c9c\|2c9c]], [[4qnm\|4qnm]], [[4qos\|4qos]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qnr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qnr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qnr RCSB], [http://www.ebi.ac.uk/pdbsum/4qnr PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Binding and hydrolysis of ATP is universally required by AAA+ proteins to underpin their mechano-chemical work. Here we explore the roles of the ATPase site in an AAA+ transcriptional activator protein, the phage shock protein F (PspF), by specifically altering the Walker B motif sequence required in catalyzing ATP hydrolysis. One such mutant, the E108Q variant, is defective in ATP hydrolysis but fully remodels target transcription complexes, the RNAP-sigma54 holoenzyme, in an ATP dependent manner. Structural analysis of the E108Q variant reveals that unlike wild-type protein, which has distinct conformations for E108 residue in the ATP and ADP bound forms, E108Q adapts the same conformation irrespective of nucleotide bound. Our data show that the remodeling activities of E108Q are strongly favored on pre-melted DNA and engagement with RNAP-sigma54 using ATP binding can be sufficient to convert the inactive holoenzyme to an active form, while hydrolysis per se is required for nucleic acid remodeling that leads to transcription bubble formation. Furthermore, using linked dimer constructs, we show that RNAP-sigma54 engagement by adjacent subunits within a hexamer are required for this protein remodeling activity while DNA remodeling activity can tolerate defective ATP hydrolysis of alternating subunits.

-	~~The entry 4qnr is ON HOLD~~	+	Molecular basis of nucleotide-dependent substrate engagement and remodeling by an AAA+ activator.,Darbari VC, Lawton E, Lu D, Burrows PC, Wiesler S, Joly N, Zhang N, Zhang X, Buck M Nucleic Acids Res. 2014 Jul 25. pii: gku588. PMID:25063294<ref>PMID:25063294</ref>

-	~~Authors~~: Darbari, V.C., Lawton, E., Lu, D.~~, Burrows, P.C.,~~ Wiesler, S.~~, Joly, N.,~~ Zhang, N., Zhang, X.~~, Buck, M.~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~CRYSTAL STRUCTURE OF PSPF(1-265) E108Q MUTANT bound to ATP~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Buck, M.]]
		+	[[Category: Burrows, P C.]]
		+	[[Category: Darbari, V C.]]
		+	[[Category: Joly, N.]]
		+	[[Category: Lawton, E.]]
		+	[[Category: Lu, D.]]
		+	[[Category: Wiesler, S.]]
		+	[[Category: Zhang, N.]]
		+	[[Category: Zhang, X.]]
		+	[[Category: Aaa domain]]
		+	[[Category: Atp binding]]
		+	[[Category: Bacterial sigma54 activator]]
		+	[[Category: Dna binding]]
		+	[[Category: Transcription]]

OCA: Protected "4qnr" [edit=sysop:move=sysop]

OCA — Wed, 25 Jun 2014 05:58:39 GMT

Protected "4qnr" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:58, 25 June 2014

OCA: New page: '''Unreleased structure''' The entry 4qnr is ON HOLD Authors: Darbari, V.C., Lawton, E., Lu, D., Burrows, P.C., Wiesler, S., Joly, N., Zhang, N., Zhang, X., Buck, M. Description: CRYST...

OCA — Wed, 25 Jun 2014 05:58:38 GMT

New page: '''Unreleased structure''' The entry 4qnr is ON HOLD Authors: Darbari, V.C., Lawton, E., Lu, D., Burrows, P.C., Wiesler, S., Joly, N., Zhang, N., Zhang, X., Buck, M. Description: CRYST...

New page

'''Unreleased structure'''

The entry 4qnr is ON HOLD

Authors: Darbari, V.C., Lawton, E., Lu, D., Burrows, P.C., Wiesler, S., Joly, N., Zhang, N., Zhang, X., Buck, M.

Description: CRYSTAL STRUCTURE OF PSPF(1-265) E108Q MUTANT bound to ATP