4qqy - Revision history

OCA at 12:48, 1 March 2024

OCA — Fri, 01 Mar 2024 12:48:08 GMT

←Older revision		Revision as of 12:48, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4qqy]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermobifida_fusca_YX Thermobifida fusca YX]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QQY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QQY FirstGlance]. <br>		<table><tr><td colspan='2'>[[4qqy]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermobifida_fusca_YX Thermobifida fusca YX]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QQY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QQY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.12Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qqy OCA], [https://pdbe.org/4qqy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qqy RCSB], [https://www.ebi.ac.uk/pdbsum/4qqy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qqy ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qqy OCA], [https://pdbe.org/4qqy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qqy RCSB], [https://www.ebi.ac.uk/pdbsum/4qqy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qqy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/Q47PJ0_THEFY Q47PJ0_THEFY]		[https://www.uniprot.org/uniprot/Q47PJ0_THEFY Q47PJ0_THEFY]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	CRISPR drives prokaryotic adaptation to invasive nucleic acids such as phages and plasmids, using an RNA-mediated interference mechanism. Interference in type I CRISPR-Cas systems requires a targeting Cascade complex and a degradation machine, Cas3, which contains both nuclease and helicase activities. Here we report the crystal structures of Thermobifida fusca Cas3 bound to single-stranded (ss) DNA substrate and show that it is an obligate 3'-to-5' ssDNase that preferentially accepts substrate directly from the helicase moiety. Conserved residues in the HD-type nuclease coordinate two irons for ssDNA cleavage. We demonstrate ATP coordination and conformational flexibility of the SF2-type helicase domain. Cas3 is specifically guided toward Cascade-bound target DNA by a PAM sequence, through physical interactions with both the nontarget substrate strand and the CasA protein. The sequence of recognition events ensures well-controlled DNA targeting and degradation of foreign DNA by Cascade and Cas3.
-
-	Structures of CRISPR Cas3 offer mechanistic insights into Cascade-activated DNA unwinding and degradation.,Huo Y, Nam KH, Ding F, Lee H, Wu L, Xiao Y, Farchione MD Jr, Zhou S, Rajashankar K, Kurinov I, Zhang R, Ke A Nat Struct Mol Biol. 2014 Aug 17. doi: 10.1038/nsmb.2875. PMID:25132177<ref>PMID:25132177</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4qqy" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 23:		Line 15:
	*[[Endonuclease 3D structures\|Endonuclease 3D structures]]		*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
	*[[Helicase 3D structures\|Helicase 3D structures]]		*[[Helicase 3D structures\|Helicase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 13:48, 22 February 2023

OCA — Wed, 22 Feb 2023 13:48:03 GMT

←Older revision		Revision as of 13:48, 22 February 2023
Line 3:		Line 3:
	<StructureSection load='4qqy' size='340' side='right'caption='[[4qqy]], [[Resolution\|resolution]] 3.12Å' scene=''>		<StructureSection load='4qqy' size='340' side='right'caption='[[4qqy]], [[Resolution\|resolution]] 3.12Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4qqy]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Thefy Thefy~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QQY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4QQY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4qqy]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermobifida_fusca_YX Thermobifida fusca YX]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QQY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QQY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qqw\|4qqw]], [[4qqz\|4qqz]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qqy OCA], [https://pdbe.org/4qqy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qqy RCSB], [https://www.ebi.ac.uk/pdbsum/4qqy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qqy ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Tfu_1593 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=269800 THEFY])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4qqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qqy OCA], [~~http~~://pdbe.org/4qqy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4qqy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4qqy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4qqy ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q47PJ0_THEFY Q47PJ0_THEFY]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	==See Also==		==See Also==
	*[[CRISPR type I-E (Cascade)\|CRISPR type I-E (Cascade)]]		*[[CRISPR type I-E (Cascade)\|CRISPR type I-E (Cascade)]]
-	*[[Endonuclease\|Endonuclease]]	+	*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
-	*[[Helicase\|Helicase]]	+	*[[Helicase 3D structures\|Helicase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Thefy~~]]	+	[[Category: Thermobifida fusca YX]]
-	[[Category: Huo, Y]]	+	[[Category: Huo Y]]
-	[[Category: Ke, A]]	+	[[Category: Ke A]]
-	[[Category: Nam~~, K H]]~~	+	[[Category: Nam KH]]
-	~~[[Category: Cas3]]~~	+
-	~~[[Category: Helicase]]~~	+
-	~~[[Category: Hydrolase-dna complex]]~~	+
-	~~[[Category: Rispr~~]]	+

OCA at 09:24, 24 April 2019

OCA — Wed, 24 Apr 2019 09:24:38 GMT

←Older revision		Revision as of 09:24, 24 April 2019
Line 1:		Line 1:

	==Crystal structure of T. fusca Cas3-ADP==		==Crystal structure of T. fusca Cas3-ADP==
-	<StructureSection load='4qqy' size='340' side='right' caption='[[4qqy]], [[Resolution\|resolution]] 3.12Å' scene=''>	+	<StructureSection load='4qqy' size='340' side='right'caption='[[4qqy]], [[Resolution\|resolution]] 3.12Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4qqy]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QQY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QQY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4qqy]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Thefy Thefy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QQY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QQY FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qqw\|4qqw]], [[4qqz\|4qqz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qqw\|4qqw]], [[4qqz\|4qqz]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Tfu_1593 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=269800 THEFY])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qqy OCA], [http://pdbe.org/4qqy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qqy RCSB], [http://www.ebi.ac.uk/pdbsum/4qqy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qqy ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qqy OCA], [http://pdbe.org/4qqy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qqy RCSB], [http://www.ebi.ac.uk/pdbsum/4qqy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qqy ProSAT]</span></td></tr>
	</table>		</table>
Line 19:		Line 20:

	==See Also==		==See Also==
		+	*[[CRISPR type I-E (Cascade)\|CRISPR type I-E (Cascade)]]
	*[[Endonuclease\|Endonuclease]]		*[[Endonuclease\|Endonuclease]]
	*[[Helicase\|Helicase]]		*[[Helicase\|Helicase]]
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Thefy]]
	[[Category: Huo, Y]]		[[Category: Huo, Y]]
	[[Category: Ke, A]]		[[Category: Ke, A]]

OCA at 11:16, 11 August 2016

OCA — Thu, 11 Aug 2016 11:16:54 GMT

←Older revision		Revision as of 11:16, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of T. fusca Cas3-ADP==		==Crystal structure of T. fusca Cas3-ADP==
	<StructureSection load='4qqy' size='340' side='right' caption='[[4qqy]], [[Resolution\|resolution]] 3.12Å' scene=''>		<StructureSection load='4qqy' size='340' side='right' caption='[[4qqy]], [[Resolution\|resolution]] 3.12Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qqw\|4qqw]], [[4qqz\|4qqz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qqw\|4qqw]], [[4qqz\|4qqz]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qqy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qqy RCSB], [http://www.ebi.ac.uk/pdbsum/4qqy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qqy OCA], [http://pdbe.org/4qqy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qqy RCSB], [http://www.ebi.ac.uk/pdbsum/4qqy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qqy ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4qqy" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Endonuclease\|Endonuclease]]		*[[Endonuclease\|Endonuclease]]
		+	*[[Helicase\|Helicase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:28, 20 January 2015

OCA — Tue, 20 Jan 2015 08:28:36 GMT

←Older revision		Revision as of 08:28, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4qqy]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QQY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QQY FirstGlance]. <br>		<table><tr><td colspan='2'>[[4qqy]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QQY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QQY FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qqw\|4qqw]], [[4qqz\|4qqz]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qqw\|4qqw]], [[4qqz\|4qqz]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qqy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qqy RCSB], [http://www.ebi.ac.uk/pdbsum/4qqy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qqy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qqy RCSB], [http://www.ebi.ac.uk/pdbsum/4qqy PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[Endonuclease\|Endonuclease]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Huo, Y.]]	+	[[Category: Huo, Y]]
-	[[Category: Ke, A.]]	+	[[Category: Ke, A]]
-	[[Category: Nam, K H.]]	+	[[Category: Nam, K H]]
	[[Category: Cas3]]		[[Category: Cas3]]
	[[Category: Helicase]]		[[Category: Helicase]]
	[[Category: Hydrolase-dna complex]]		[[Category: Hydrolase-dna complex]]
	[[Category: Rispr]]		[[Category: Rispr]]

OCA at 08:45, 27 August 2014

OCA — Wed, 27 Aug 2014 08:45:26 GMT

←Older revision		Revision as of 08:45, 27 August 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of T. fusca Cas3-ADP==
		+	<StructureSection load='4qqy' size='340' side='right' caption='[[4qqy]], [[Resolution\|resolution]] 3.12Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4qqy]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QQY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QQY FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qqw\|4qqw]], [[4qqz\|4qqz]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qqy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qqy RCSB], [http://www.ebi.ac.uk/pdbsum/4qqy PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	CRISPR drives prokaryotic adaptation to invasive nucleic acids such as phages and plasmids, using an RNA-mediated interference mechanism. Interference in type I CRISPR-Cas systems requires a targeting Cascade complex and a degradation machine, Cas3, which contains both nuclease and helicase activities. Here we report the crystal structures of Thermobifida fusca Cas3 bound to single-stranded (ss) DNA substrate and show that it is an obligate 3'-to-5' ssDNase that preferentially accepts substrate directly from the helicase moiety. Conserved residues in the HD-type nuclease coordinate two irons for ssDNA cleavage. We demonstrate ATP coordination and conformational flexibility of the SF2-type helicase domain. Cas3 is specifically guided toward Cascade-bound target DNA by a PAM sequence, through physical interactions with both the nontarget substrate strand and the CasA protein. The sequence of recognition events ensures well-controlled DNA targeting and degradation of foreign DNA by Cascade and Cas3.

-	~~The entry 4qqy is ON HOLD~~	+	Structures of CRISPR Cas3 offer mechanistic insights into Cascade-activated DNA unwinding and degradation.,Huo Y, Nam KH, Ding F, Lee H, Wu L, Xiao Y, Farchione MD Jr, Zhou S, Rajashankar K, Kurinov I, Zhang R, Ke A Nat Struct Mol Biol. 2014 Aug 17. doi: 10.1038/nsmb.2875. PMID:25132177<ref>PMID:25132177</ref>

-	~~Authors: Ke~~, A., Huo, Y., Nam, K.H.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of T. fusca~~ Cas3-~~ADP~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Huo, Y.]]
		+	[[Category: Ke, A.]]
		+	[[Category: Nam, K H.]]
		+	[[Category: Cas3]]
		+	[[Category: Helicase]]
		+	[[Category: Hydrolase-dna complex]]
		+	[[Category: Rispr]]

OCA at 07:40, 13 August 2014

OCA — Wed, 13 Aug 2014 07:40:02 GMT

←Older revision		Revision as of 07:40, 13 August 2014
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4qqy is ON HOLD ~~until sometime in the future~~	+	The entry 4qqy is ON HOLD

	Authors: Ke, A., Huo, Y., Nam, K.H.		Authors: Ke, A., Huo, Y., Nam, K.H.

	Description: Crystal structure of T. fusca Cas3-ADP		Description: Crystal structure of T. fusca Cas3-ADP

OCA at 07:11, 23 July 2014

OCA — Wed, 23 Jul 2014 07:11:58 GMT

←Older revision		Revision as of 07:11, 23 July 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4qqy is ON HOLD	+	The entry 4qqy is ON HOLD until sometime in the future

	Authors: Ke, A., Huo, Y., Nam, K.H.		Authors: Ke, A., Huo, Y., Nam, K.H.

	Description: Crystal structure of T. fusca Cas3-ADP		Description: Crystal structure of T. fusca Cas3-ADP

OCA: Protected "4qqy" [edit=sysop:move=sysop]

OCA — Wed, 09 Jul 2014 06:52:55 GMT

Protected "4qqy" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:52, 9 July 2014

OCA: New page: '''Unreleased structure''' The entry 4qqy is ON HOLD Authors: Ke, A., Huo, Y., Nam, K.H. Description: Crystal structure of T. fusca Cas3-ADP

OCA — Wed, 09 Jul 2014 06:52:55 GMT

New page: '''Unreleased structure''' The entry 4qqy is ON HOLD Authors: Ke, A., Huo, Y., Nam, K.H. Description: Crystal structure of T. fusca Cas3-ADP

New page

'''Unreleased structure'''

The entry 4qqy is ON HOLD

Authors: Ke, A., Huo, Y., Nam, K.H.

Description: Crystal structure of T. fusca Cas3-ADP