4r0m - Revision history

OCA at 14:02, 22 February 2023

OCA — Wed, 22 Feb 2023 14:02:53 GMT

←Older revision		Revision as of 14:02, 22 February 2023
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	<StructureSection load='4r0m' size='340' side='right'caption='[[4r0m]], [[Resolution\|resolution]] 2.45Å' scene=''>		<StructureSection load='4r0m' size='340' side='right'caption='[[4r0m]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4r0m]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Microcystis_aeruginosa_pcc_7806~~ Microcystis aeruginosa ~~pcc~~ 7806]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R0M OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4R0M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4r0m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Microcystis_aeruginosa_PCC_7806 Microcystis aeruginosa PCC 7806]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R0M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R0M FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FA5:ADENOSINE-5-[PHENYLALANINYL-PHOSPHATE]'>FA5</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FA5:ADENOSINE-5-[PHENYLALANINYL-PHOSPHATE]'>FA5</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>~~</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r0m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r0m OCA], [https://pdbe.org/4r0m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r0m RCSB], [https://www.ebi.ac.uk/pdbsum/4r0m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r0m ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mcyG, IPF_370 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=267872 Microcystis aeruginosa PCC 7806])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4r0m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r0m OCA], [~~http~~://pdbe.org/4r0m PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4r0m RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4r0m PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4r0m ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A8YJW1_MICAE A8YJW1_MICAE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Microcystis aeruginosa ~~pcc~~ 7806]]	+	[[Category: Microcystis aeruginosa PCC 7806]]
-	[[Category: Chen~~, Y X~~]]	+	[[Category: Chen YX]]
-	[[Category: Dai~~, Y N~~]]	+	[[Category: Dai YN]]
-	[[Category: Jiang~~, Y L~~]]	+	[[Category: Jiang YL]]
-	[[Category: Ren~~, Y M~~]]	+	[[Category: Ren YM]]
-	[[Category: Tan~~, X F~~]]	+	[[Category: Tan XF]]
-	[[Category: Zhou~~, C Z~~]]	+	[[Category: Zhou CZ]]
-	[[Category: Zhou, K]]	+	[[Category: Zhou K]]
-	~~[[Category: Acetyl-coa synthetase-like domain]]~~	+
-	~~[[Category: Acyl carrier protein-like domain]]~~	+
-	~~[[Category: Adenylation domain]]~~	+
-	~~[[Category: Ligase]]~~	+
-	~~[[Category: Peptidyl carrier protein like domain]]~~	+
-	~~[[Category: Phenylalanyl-amp]]~~	+
-	~~[[Category: Phenylalanyl-amp binding~~]]	+

OCA at 08:23, 18 December 2019

OCA — Wed, 18 Dec 2019 08:23:24 GMT

←Older revision		Revision as of 08:23, 18 December 2019
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r0m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r0m OCA], [http://pdbe.org/4r0m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r0m RCSB], [http://www.ebi.ac.uk/pdbsum/4r0m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r0m ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r0m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r0m OCA], [http://pdbe.org/4r0m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r0m RCSB], [http://www.ebi.ac.uk/pdbsum/4r0m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r0m ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Microcystins, which are the most common cause of hepatotoxicity associated with cyanobacterial water blooms, are assembled in vivo on a large multienzyme complex via a mixed nonribosomal peptide synthetase/polyketide synthetase (NRPS/PKS). The biosynthesis of microcystin in Microcystis aeruginosa PCC 7806 starts with the enzyme McyG, which contains an adenylation-peptidyl carrier protein (A-PCP) didomain for loading the starter unit to assemble the side chain of an Adda residue. However, the catalytic mechanism remains unclear. Here, the 2.45 A resolution crystal structure of the McyG A-PCP didomain complexed with the catalytic intermediate L-phenylalanyl-adenylate (L-Phe-AMP) is reported. Each asymmetric unit contains two protein molecules, one of which consists of the A-PCP didomain and the other of which comprises only the A domain. Structural analyses suggest that Val227 is likely to be critical for the selection of hydrophobic substrates. Moreover, two distinct interfaces demonstrating variable crosstalk between the PCP domain and the A domain were observed. A catalytic cycle for the adenylation and peptide transfer of the A-PCP didomain is proposed.
		+
		+	Structure of the adenylation-peptidyl carrier protein didomain of the Microcystis aeruginosa microcystin synthetase McyG.,Tan XF, Dai YN, Zhou K, Jiang YL, Ren YM, Chen Y, Zhou CZ Acta Crystallogr D Biol Crystallogr. 2015 Apr;71(Pt 4):873-81. doi:, 10.1107/S1399004715001716. Epub 2015 Mar 27. PMID:25849398<ref>PMID:25849398</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4r0m" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:58, 24 April 2019

OCA — Wed, 24 Apr 2019 08:58:25 GMT

←Older revision		Revision as of 08:58, 24 April 2019
Line 1:		Line 1:

	==Structure of McyG A-PCP complexed with phenylalanyl-adenylate==		==Structure of McyG A-PCP complexed with phenylalanyl-adenylate==
-	<StructureSection load='4r0m' size='340' side='right' caption='[[4r0m]], [[Resolution\|resolution]] 2.45Å' scene=''>	+	<StructureSection load='4r0m' size='340' side='right'caption='[[4r0m]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4r0m]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R0M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R0M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4r0m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Microcystis_aeruginosa_pcc_7806 Microcystis aeruginosa pcc 7806]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R0M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R0M FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FA5:ADENOSINE-5-[PHENYLALANINYL-PHOSPHATE]'>FA5</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FA5:ADENOSINE-5-[PHENYLALANINYL-PHOSPHATE]'>FA5</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mcyG, IPF_370 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=267872 Microcystis aeruginosa PCC 7806])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r0m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r0m OCA], [http://pdbe.org/4r0m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r0m RCSB], [http://www.ebi.ac.uk/pdbsum/4r0m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r0m ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r0m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r0m OCA], [http://pdbe.org/4r0m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r0m RCSB], [http://www.ebi.ac.uk/pdbsum/4r0m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r0m ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Microcystis aeruginosa pcc 7806]]
	[[Category: Chen, Y X]]		[[Category: Chen, Y X]]
	[[Category: Dai, Y N]]		[[Category: Dai, Y N]]

OCA at 17:28, 18 January 2017

OCA — Wed, 18 Jan 2017 17:28:52 GMT

←Older revision		Revision as of 17:28, 18 January 2017
Line 1:		Line 1:
		+
	==Structure of McyG A-PCP complexed with phenylalanyl-adenylate==		==Structure of McyG A-PCP complexed with phenylalanyl-adenylate==
	<StructureSection load='4r0m' size='340' side='right' caption='[[4r0m]], [[Resolution\|resolution]] 2.45Å' scene=''>		<StructureSection load='4r0m' size='340' side='right' caption='[[4r0m]], [[Resolution\|resolution]] 2.45Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FA5:ADENOSINE-5-[PHENYLALANINYL-PHOSPHATE]'>FA5</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FA5:ADENOSINE-5-[PHENYLALANINYL-PHOSPHATE]'>FA5</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r0m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r0m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r0m RCSB], [http://www.ebi.ac.uk/pdbsum/4r0m PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r0m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r0m OCA], [http://pdbe.org/4r0m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r0m RCSB], [http://www.ebi.ac.uk/pdbsum/4r0m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r0m ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 12:59, 15 April 2015

OCA — Wed, 15 Apr 2015 12:59:43 GMT

←Older revision		Revision as of 12:59, 15 April 2015
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-	'''~~Unreleased structure~~'''	+	==Structure of McyG A-PCP complexed with phenylalanyl-adenylate==
-		+	<StructureSection load='4r0m' size='340' side='right' caption='[[4r0m]], [[Resolution\|resolution]] 2.45Å' scene=''>
-	~~The entry~~ 4r0m is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4r0m]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R0M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R0M FirstGlance]. <br>
-	~~Authors~~: ~~Tan, X~~.F.~~, Dai, Y~~.N., ~~Zhou, K~~.~~, Jiang, Y~~.L., ~~Ren, Y~~.M., Chen, Y.X., ~~Zhou~~, ~~C.Z.~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FA5:ADENOSINE-5-[PHENYLALANINYL-PHOSPHATE]'>FA5</scene></td></tr>
-		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~Description~~: ~~Structure of McyG A-PCP complexed with phenylalanyl-adenylate~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r0m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r0m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r0m RCSB], [http://www.ebi.ac.uk/pdbsum/4r0m PDBsum]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chen, Y X]]
		+	[[Category: Dai, Y N]]
		+	[[Category: Jiang, Y L]]
		+	[[Category: Ren, Y M]]
		+	[[Category: Tan, X F]]
		+	[[Category: Zhou, C Z]]
	[[Category: Zhou, K]]		[[Category: Zhou, K]]
-	[[Category: ~~Chen, Y.X~~]]	+	[[Category: Acetyl-coa synthetase-like domain]]
-	[[Category: ~~Dai, Y.N~~]]	+	[[Category: Acyl carrier protein-like domain]]
-	[[Category: ~~Tan, X.F~~]]	+	[[Category: Adenylation domain]]
-	[[Category: ~~Ren, Y.M~~]]	+	[[Category: Ligase]]
-	[[Category: ~~Jiang, Y.L~~]]	+	[[Category: Peptidyl carrier protein like domain]]
-	[[Category: ~~Zhou, C.Z~~]]	+	[[Category: Phenylalanyl-amp]]
		+	[[Category: Phenylalanyl-amp binding]]

OCA at 08:09, 12 February 2015

OCA — Thu, 12 Feb 2015 08:09:49 GMT

←Older revision		Revision as of 08:09, 12 February 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4r0m is ON HOLD	+	The entry 4r0m is ON HOLD until Paper Publication

	Authors: Tan, X.F., Dai, Y.N., Zhou, K., Jiang, Y.L., Ren, Y.M., Chen, Y.X., Zhou, C.Z.		Authors: Tan, X.F., Dai, Y.N., Zhou, K., Jiang, Y.L., Ren, Y.M., Chen, Y.X., Zhou, C.Z.

OCA at 16:20, 24 December 2014

OCA — Wed, 24 Dec 2014 16:20:27 GMT

←Older revision		Revision as of 16:20, 24 December 2014
Line 6:		Line 6:

	Description: Structure of McyG A-PCP complexed with phenylalanyl-adenylate		Description: Structure of McyG A-PCP complexed with phenylalanyl-adenylate
		+	[[Category: Unreleased Structures]]
		+	[[Category: Zhou, K]]
		+	[[Category: Chen, Y.X]]
		+	[[Category: Dai, Y.N]]
		+	[[Category: Tan, X.F]]
		+	[[Category: Ren, Y.M]]
		+	[[Category: Jiang, Y.L]]
		+	[[Category: Zhou, C.Z]]

OCA: Protected "4r0m" [edit=sysop:move=sysop]

OCA — Wed, 13 Aug 2014 07:46:51 GMT

Protected "4r0m" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:46, 13 August 2014

OCA: New page: '''Unreleased structure''' The entry 4r0m is ON HOLD Authors: Tan, X.F., Dai, Y.N., Zhou, K., Jiang, Y.L., Ren, Y.M., Chen, Y.X., Zhou, C.Z. Description: Structure of McyG A-PCP comple...

OCA — Wed, 13 Aug 2014 07:46:49 GMT

New page: '''Unreleased structure''' The entry 4r0m is ON HOLD Authors: Tan, X.F., Dai, Y.N., Zhou, K., Jiang, Y.L., Ren, Y.M., Chen, Y.X., Zhou, C.Z. Description: Structure of McyG A-PCP comple...

New page

'''Unreleased structure'''

The entry 4r0m is ON HOLD

Authors: Tan, X.F., Dai, Y.N., Zhou, K., Jiang, Y.L., Ren, Y.M., Chen, Y.X., Zhou, C.Z.

Description: Structure of McyG A-PCP complexed with phenylalanyl-adenylate