4r1t - Revision history

OCA at 14:05, 22 February 2023

OCA — Wed, 22 Feb 2023 14:05:57 GMT

←Older revision		Revision as of 14:05, 22 February 2023
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	<StructureSection load='4r1t' size='340' side='right'caption='[[4r1t]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='4r1t' size='340' side='right'caption='[[4r1t]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4r1t]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pethy Pethy~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R1T OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4R1T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4r1t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Petunia_x_hybrida Petunia x hybrida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R1T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R1T FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=I2I:MOLECULAR+IODINE'>I2I</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=I2I:MOLECULAR+IODINE'>I2I</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4r1s\|4r1s]], [[4r1u\|4r1u]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r1t OCA], [https://pdbe.org/4r1t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r1t RCSB], [https://www.ebi.ac.uk/pdbsum/4r1t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r1t ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PhCCR1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4102 PETHY])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4r1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r1t OCA], [~~http~~://pdbe.org/4r1t PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4r1t RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4r1t PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4r1t ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CCR1_PETHY CCR1_PETHY] Involved in the latter stages of lignin biosynthesis (PubMed:24985707). Catalyzes one of the last steps of monolignol biosynthesis, the conversion of cinnamoyl-CoAs into their corresponding cinnamaldehydes (PubMed:25217505, PubMed:24985707). Mediates the conversion of feruloyl CoA to coniferylaldehyde (PubMed:25217505, PubMed:24985707). Also active toward p-coumaroyl-CoA and sinapoyl-CoA (PubMed:25217505, PubMed:24985707). Involved in the production of floral volatile phenylpropanoids in flowers of fragrant cultivars (e.g. cv. Mitchell and cv. V26) from cinnamic acid, a common precursor with the anthocyanin biosynthesis pathway involved in flower pigmentation (PubMed:24985707).<ref>PMID:24985707</ref> <ref>PMID:25217505</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pethy~~]]	+	[[Category: Petunia x hybrida]]
-	[[Category: Bomati~~, E K~~]]	+	[[Category: Bomati EK]]
-	[[Category: Bowman~~, M E~~]]	+	[[Category: Bowman ME]]
-	[[Category: Louie~~, G V~~]]	+	[[Category: Louie GV]]
-	[[Category: Noel~~, J P]]~~	+	[[Category: Noel JP]]
-	~~[[Category: Cinnamoyl-coa reductase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Short-chain dehydrogenase/reductase~~]]	+

OCA at 08:35, 24 April 2019

OCA — Wed, 24 Apr 2019 08:35:48 GMT

←Older revision		Revision as of 08:35, 24 April 2019
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	==Crystal structure of Petunia hydrida cinnamoyl-CoA reductase==		==Crystal structure of Petunia hydrida cinnamoyl-CoA reductase==
-	<StructureSection load='4r1t' size='340' side='right' caption='[[4r1t]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='4r1t' size='340' side='right'caption='[[4r1t]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4r1t]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R1T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R1T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4r1t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pethy Pethy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R1T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R1T FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=I2I:MOLECULAR+IODINE'>I2I</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=I2I:MOLECULAR+IODINE'>I2I</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4r1s\|4r1s]], [[4r1u\|4r1u]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4r1s\|4r1s]], [[4r1u\|4r1u]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PhCCR1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4102 PETHY])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r1t OCA], [http://pdbe.org/4r1t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r1t RCSB], [http://www.ebi.ac.uk/pdbsum/4r1t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r1t ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r1t OCA], [http://pdbe.org/4r1t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r1t RCSB], [http://www.ebi.ac.uk/pdbsum/4r1t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r1t ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Pethy]]
	[[Category: Bomati, E K]]		[[Category: Bomati, E K]]
	[[Category: Bowman, M E]]		[[Category: Bowman, M E]]

OCA at 08:06, 11 August 2016

OCA — Thu, 11 Aug 2016 08:06:53 GMT

←Older revision		Revision as of 08:06, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Petunia hydrida cinnamoyl-CoA reductase==		==Crystal structure of Petunia hydrida cinnamoyl-CoA reductase==
	<StructureSection load='4r1t' size='340' side='right' caption='[[4r1t]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='4r1t' size='340' side='right' caption='[[4r1t]], [[Resolution\|resolution]] 1.70Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=I2I:MOLECULAR+IODINE'>I2I</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=I2I:MOLECULAR+IODINE'>I2I</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4r1s\|4r1s]], [[4r1u\|4r1u]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4r1s\|4r1s]], [[4r1u\|4r1u]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r1t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r1t RCSB], [http://www.ebi.ac.uk/pdbsum/4r1t PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r1t OCA], [http://pdbe.org/4r1t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r1t RCSB], [http://www.ebi.ac.uk/pdbsum/4r1t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r1t ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4r1t" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:46, 12 November 2014

OCA — Wed, 12 Nov 2014 07:46:58 GMT

←Older revision		Revision as of 07:46, 12 November 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4r1t]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R1T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R1T FirstGlance]. <br>		<table><tr><td colspan='2'>[[4r1t]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R1T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R1T FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=I2I:MOLECULAR+IODINE'>I2I</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=I2I:MOLECULAR+IODINE'>I2I</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4r1s\|4r1s]], [[4r1u\|4r1u]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4r1s\|4r1s]], [[4r1u\|4r1u]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r1t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r1t RCSB], [http://www.ebi.ac.uk/pdbsum/4r1t PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r1t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r1t RCSB], [http://www.ebi.ac.uk/pdbsum/4r1t PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bomati, E K.]]	+	[[Category: Bomati, E K]]
-	[[Category: Bowman, M E.]]	+	[[Category: Bowman, M E]]
-	[[Category: Louie, G V.]]	+	[[Category: Louie, G V]]
-	[[Category: Noel, J P.]]	+	[[Category: Noel, J P]]
	[[Category: Cinnamoyl-coa reductase]]		[[Category: Cinnamoyl-coa reductase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Short-chain dehydrogenase/reductase]]		[[Category: Short-chain dehydrogenase/reductase]]

OCA at 00:10, 2 October 2014

OCA — Thu, 02 Oct 2014 00:10:31 GMT

←Older revision		Revision as of 00:10, 2 October 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of Petunia hydrida cinnamoyl-CoA reductase==
		+	<StructureSection load='4r1t' size='340' side='right' caption='[[4r1t]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4r1t]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R1T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R1T FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=I2I:MOLECULAR+IODINE'>I2I</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4r1s\|4r1s]], [[4r1u\|4r1u]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r1t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r1t RCSB], [http://www.ebi.ac.uk/pdbsum/4r1t PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The enzymes cinnamoyl-CoA reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD) catalyze the two key reduction reactions in the conversion of cinnamic acid derivatives into monolignol building blocks for lignin polymers in plant cell walls. Here, we describe detailed functional and structural analyses of CCRs from Medicago truncatula and Petunia hybrida and of an atypical CAD (CAD2) from M. truncatula. These enzymes are closely related members of the short-chain dehydrogenase/reductase (SDR) superfamily. Our structural studies support a reaction mechanism involving a canonical SDR catalytic triad in both CCR and CAD2 and an important role for an auxiliary cysteine unique to CCR. Site-directed mutants of CAD2 (Phe226Ala and Tyr136Phe) that enlarge the phenolic binding site result in a 4- to 10-fold increase in activity with sinapaldehyde, which in comparison to the smaller coumaraldehyde and coniferaldehyde substrates is disfavored by wild-type CAD2. This finding demonstrates the potential exploitation of rationally engineered forms of CCR and CAD2 for the targeted modification of monolignol composition in transgenic plants. Thermal denaturation measurements and structural comparisons of various liganded and unliganded forms of CCR and CAD2 highlight substantial conformational flexibility of these SDR enzymes, which plays an important role in the establishment of catalytically productive complexes of the enzymes with their NADPH and phenolic substrates.

-	~~The entry 4r1t is ON HOLD until Paper Publication~~	+	Structural Studies of Cinnamoyl-CoA Reductase and Cinnamyl-Alcohol Dehydrogenase, Key Enzymes of Monolignol Biosynthesis.,Pan H, Zhou R, Louie GV, Muhlemann JK, Bomati EK, Bowman ME, Dudareva N, Dixon RA, Noel JP, Wang X Plant Cell. 2014 Sep 12. pii: tpc.114.127399. PMID:25217505<ref>PMID:25217505</ref>

-	~~Authors: Noel~~, J.P.~~, Louie, G.V~~., Bowman, M.E., ~~Bomati~~, ~~E.K~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of Petunia hydrida cinnamoyl~~-~~CoA~~ reductase	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bomati, E K.]]
		+	[[Category: Bowman, M E.]]
		+	[[Category: Louie, G V.]]
		+	[[Category: Noel, J P.]]
		+	[[Category: Cinnamoyl-coa reductase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Short-chain dehydrogenase/reductase]]

OCA at 08:20, 24 September 2014

OCA — Wed, 24 Sep 2014 08:20:14 GMT

←Older revision		Revision as of 08:20, 24 September 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4r1t is ON HOLD	+	The entry 4r1t is ON HOLD until Paper Publication

	Authors: Noel, J.P., Louie, G.V., Bowman, M.E., Bomati, E.K.		Authors: Noel, J.P., Louie, G.V., Bowman, M.E., Bomati, E.K.

	Description: Crystal structure of Petunia hydrida cinnamoyl-CoA reductase		Description: Crystal structure of Petunia hydrida cinnamoyl-CoA reductase

OCA at 14:45, 20 August 2014

OCA — Wed, 20 Aug 2014 14:45:09 GMT

←Older revision		Revision as of 14:45, 20 August 2014
Line 3:		Line 3:
	The entry 4r1t is ON HOLD		The entry 4r1t is ON HOLD

-	Authors: Noel, ~~Joseph~~ P., Louie, ~~Gordon~~ V., Bowman, ~~Marianne~~ E., Bomati, ~~Erin~~ K.	+	Authors: Noel, J.P., Louie, G.V., Bowman, M.E., Bomati, E.K.

	Description: Crystal structure of Petunia hydrida cinnamoyl-CoA reductase		Description: Crystal structure of Petunia hydrida cinnamoyl-CoA reductase

OCA: Protected "4r1t" [edit=sysop:move=sysop]

OCA — Wed, 13 Aug 2014 07:49:23 GMT

Protected "4r1t" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:49, 13 August 2014

OCA: New page: '''Unreleased structure''' The entry 4r1t is ON HOLD Authors: Noel, Joseph P., Louie, Gordon V., Bowman, Marianne E., Bomati, Erin K. Description: Crystal structure of Petunia hydrida ...

OCA — Wed, 13 Aug 2014 07:49:22 GMT

New page: '''Unreleased structure''' The entry 4r1t is ON HOLD Authors: Noel, Joseph P., Louie, Gordon V., Bowman, Marianne E., Bomati, Erin K. Description: Crystal structure of Petunia hydrida ...

New page

'''Unreleased structure'''

The entry 4r1t is ON HOLD

Authors: Noel, Joseph P., Louie, Gordon V., Bowman, Marianne E., Bomati, Erin K.

Description: Crystal structure of Petunia hydrida cinnamoyl-CoA reductase