4r6i - Revision history

OCA at 06:42, 2 March 2023

OCA — Thu, 02 Mar 2023 06:42:07 GMT

←Older revision		Revision as of 06:42, 2 March 2023
Line 1:		Line 1:

	==AtxA protein, a virulence regulator from Bacillus anthracis.==		==AtxA protein, a virulence regulator from Bacillus anthracis.==
-	<StructureSection load='4r6i' size='340' side='right' caption='[[4r6i]], [[Resolution\|resolution]] 2.65Å' scene=''>	+	<StructureSection load='4r6i' size='340' side='right'caption='[[4r6i]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4r6i]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bacillus_anthracis_ames_"ancestor"~~ Bacillus anthracis ~~ames "ancestor"~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R6I OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4R6I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4r6i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis_str._'Ames_Ancestor' Bacillus anthracis str. 'Ames Ancestor']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R6I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R6I FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>~~</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r6i OCA], [https://pdbe.org/4r6i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r6i RCSB], [https://www.ebi.ac.uk/pdbsum/4r6i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r6i ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">atxA, BXA0146, GBAA_pXO1_0146, pXO1-119 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=261594 Bacillus anthracis Ames "Ancestor"])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4r6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r6i OCA], [~~http~~://pdbe.org/4r6i PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4r6i RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4r6i PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4r6i ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ATXA_BACAN ATXA_BACAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus anthracis ~~ames ancestor~~]]	+	[[Category: Bacillus anthracis str. 'Ames Ancestor']]
-	[[Category: ~~Anderson, W F~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Structural genomic~~]]	+	[[Category: Anderson WF]]
-	[[Category: Horton~~, L B~~]]	+	[[Category: Horton LB]]
-	[[Category: Joachimiak, A]]	+	[[Category: Joachimiak A]]
-	[[Category: Koehler~~, T M~~]]	+	[[Category: Koehler TM]]
-	[[Category: Osipiuk, J]]	+	[[Category: Osipiuk J]]
-	~~[[Category: Atxa]]~~	+
-	~~[[Category: Csgid]]~~	+
-	~~[[Category: Dna binding]]~~	+
-	~~[[Category: Idp01169]]~~	+
-	~~[[Category: National institute of allergy and infectious disease]]~~	+
-	~~[[Category: Niaid]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcriptional activator]]~~	+
-	~~[[Category: Virulence regulator~~]]	+

OCA at 09:02, 22 November 2017

OCA — Wed, 22 Nov 2017 09:02:06 GMT

←Older revision		Revision as of 09:02, 22 November 2017
Line 3:		Line 3:
	<StructureSection load='4r6i' size='340' side='right' caption='[[4r6i]], [[Resolution\|resolution]] 2.65Å' scene=''>		<StructureSection load='4r6i' size='340' side='right' caption='[[4r6i]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4r6i]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R6I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R6I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4r6i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis_ames_"ancestor" Bacillus anthracis ames "ancestor"]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R6I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R6I FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">atxA, BXA0146, GBAA_pXO1_0146, pXO1-119 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=261594 Bacillus anthracis Ames "Ancestor"])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r6i OCA], [http://pdbe.org/4r6i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r6i RCSB], [http://www.ebi.ac.uk/pdbsum/4r6i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r6i ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r6i OCA], [http://pdbe.org/4r6i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r6i RCSB], [http://www.ebi.ac.uk/pdbsum/4r6i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r6i ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus anthracis ames ancestor]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 17:16, 18 January 2017

OCA — Wed, 18 Jan 2017 17:16:54 GMT

←Older revision		Revision as of 17:16, 18 January 2017
Line 1:		Line 1:
		+
	==AtxA protein, a virulence regulator from Bacillus anthracis.==		==AtxA protein, a virulence regulator from Bacillus anthracis.==
	<StructureSection load='4r6i' size='340' side='right' caption='[[4r6i]], [[Resolution\|resolution]] 2.65Å' scene=''>		<StructureSection load='4r6i' size='340' side='right' caption='[[4r6i]], [[Resolution\|resolution]] 2.65Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r6i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r6i RCSB], [http://www.ebi.ac.uk/pdbsum/4r6i PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r6i OCA], [http://pdbe.org/4r6i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r6i RCSB], [http://www.ebi.ac.uk/pdbsum/4r6i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r6i ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4r6i" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:29, 3 December 2014

OCA — Wed, 03 Dec 2014 07:29:40 GMT

←Older revision		Revision as of 07:29, 3 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r6i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r6i RCSB], [http://www.ebi.ac.uk/pdbsum/4r6i PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r6i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r6i RCSB], [http://www.ebi.ac.uk/pdbsum/4r6i PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Bacillus anthracis virulence regulator AtxA controls transcription of the anthrax toxin genes and capsule biosynthesis operon. AtxA activity is elevated during growth in media containing glucose and CO2 /bicarbonate, and there is a positive correlation between the CO2 /bicarbonate signal, AtxA activity, and homomultimerization. AtxA activity is also affected by phosphorylation at specific histidines. We show that AtxA crystallizes as a dimer. Distinct folds associated with predicted DNA-binding domains (HTH1 and HTH2) and phosphoenolpyruvate: carbohydrate phosphotransferase system-regulated domains (PRD1 and PRD2) are apparent. We tested AtxA variants containing single and double phosphomimetic (His-->Asp) and phosphoablative (His-->Ala) amino acid changes for activity in B. anthracis cultures and for protein-protein interactions in cell lysates. Reduced activity of AtxA H199A, lack of multimerization and activity of AtxAH379D variants, and predicted structural changes associated with phosphorylation support a model for control of AtxA function. We propose that (1) in the AtxA dimer, phosphorylation of H199 in PRD1 affects HTH2 positioning, influencing DNA-binding; and (2) phosphorylation of H379 in PRD2 disrupts dimer formation. The AtxA structure is the first reported high-resolution full-length structure of a PRD-containing regulator and can serve as a model for proteins of this family, especially those that link virulence to bacterial metabolism.
		+
		+	Crystal structure of Bacillus anthracis virulence regulator AtxA and effects of phosphorylated histidines on multimerization and activity.,Hammerstrom TG, Horton LB, Swick MC, Joachimiak A, Osipiuk J, Koehler TM Mol Microbiol. 2014 Nov 17. doi: 10.1111/mmi.12867. PMID:25402841<ref>PMID:25402841</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Anderson, W F.]]	+	[[Category: Anderson, W F]]
-	[[Category: ~~CSGID, Center for~~ Structural ~~Genomics of Infectious Diseases.~~]]	+	[[Category: Structural genomic]]
-	[[Category: Horton, L B.]]	+	[[Category: Horton, L B]]
-	[[Category: Joachimiak, A.]]	+	[[Category: Joachimiak, A]]
-	[[Category: Koehler, T M.]]	+	[[Category: Koehler, T M]]
-	[[Category: Osipiuk, J.]]	+	[[Category: Osipiuk, J]]
	[[Category: Atxa]]		[[Category: Atxa]]
-	[[Category: Center for structural genomics of infectious disease]]
	[[Category: Csgid]]		[[Category: Csgid]]
	[[Category: Dna binding]]		[[Category: Dna binding]]
Line 22:		Line 31:
	[[Category: National institute of allergy and infectious disease]]		[[Category: National institute of allergy and infectious disease]]
	[[Category: Niaid]]		[[Category: Niaid]]
-	[[Category: Structural genomic]]
	[[Category: Transcription]]		[[Category: Transcription]]
	[[Category: Transcriptional activator]]		[[Category: Transcriptional activator]]
	[[Category: Virulence regulator]]		[[Category: Virulence regulator]]

OCA at 11:46, 22 October 2014

OCA — Wed, 22 Oct 2014 11:46:06 GMT

←Older revision		Revision as of 11:46, 22 October 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==AtxA protein, a virulence regulator from Bacillus anthracis.==
-		+	<StructureSection load='4r6i' size='340' side='right' caption='[[4r6i]], [[Resolution\|resolution]] 2.65Å' scene=''>
-	~~The entry~~ 4r6i is ~~ON HOLD until Oct 20 2015~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4r6i]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R6I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R6I FirstGlance]. <br>
-	~~Authors~~: ~~Osipiuk, J~~., ~~Horton, L~~.B., ~~Koehler, T~~.M., Anderson, W.F.~~, Joachimiak, A.~~, Center for Structural Genomics of Infectious Diseases ~~(CSGID)~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene></td></tr>
-		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~Description~~: ~~AtxA protein~~, ~~a virulence regulator from Bacillus anthracis~~. ~~(CASP Target)~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r6i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r6i RCSB], [http://www.ebi.ac.uk/pdbsum/4r6i PDBsum]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Anderson, W F.]]
		+	[[Category: CSGID, Center for Structural Genomics of Infectious Diseases.]]
		+	[[Category: Horton, L B.]]
		+	[[Category: Joachimiak, A.]]
		+	[[Category: Koehler, T M.]]
		+	[[Category: Osipiuk, J.]]
		+	[[Category: Atxa]]
		+	[[Category: Center for structural genomics of infectious disease]]
		+	[[Category: Csgid]]
		+	[[Category: Dna binding]]
		+	[[Category: Idp01169]]
		+	[[Category: National institute of allergy and infectious disease]]
		+	[[Category: Niaid]]
		+	[[Category: Structural genomic]]
		+	[[Category: Transcription]]
		+	[[Category: Transcriptional activator]]
		+	[[Category: Virulence regulator]]

OCA at 08:23, 10 September 2014

OCA — Wed, 10 Sep 2014 08:23:30 GMT

←Older revision		Revision as of 08:23, 10 September 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4r6i is ON HOLD	+	The entry 4r6i is ON HOLD until Oct 20 2015

	Authors: Osipiuk, J., Horton, L.B., Koehler, T.M., Anderson, W.F., Joachimiak, A., Center for Structural Genomics of Infectious Diseases (CSGID)		Authors: Osipiuk, J., Horton, L.B., Koehler, T.M., Anderson, W.F., Joachimiak, A., Center for Structural Genomics of Infectious Diseases (CSGID)

	Description: AtxA protein, a virulence regulator from Bacillus anthracis. (CASP Target)		Description: AtxA protein, a virulence regulator from Bacillus anthracis. (CASP Target)

OCA: Protected "4r6i" [edit=sysop:move=sysop]

OCA — Thu, 04 Sep 2014 04:17:00 GMT

Protected "4r6i" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:17, 4 September 2014

OCA: New page: '''Unreleased structure''' The entry 4r6i is ON HOLD Authors: Osipiuk, J., Horton, L.B., Koehler, T.M., Anderson, W.F., Joachimiak, A., Center for Structural Genomics of Infectious Dise...

OCA — Thu, 04 Sep 2014 04:16:59 GMT

New page: '''Unreleased structure''' The entry 4r6i is ON HOLD Authors: Osipiuk, J., Horton, L.B., Koehler, T.M., Anderson, W.F., Joachimiak, A., Center for Structural Genomics of Infectious Dise...

New page

'''Unreleased structure'''

The entry 4r6i is ON HOLD

Authors: Osipiuk, J., Horton, L.B., Koehler, T.M., Anderson, W.F., Joachimiak, A., Center for Structural Genomics of Infectious Diseases (CSGID)

Description: AtxA protein, a virulence regulator from Bacillus anthracis. (CASP Target)