4r7y - Revision history

OCA at 17:45, 20 September 2023

OCA — Wed, 20 Sep 2023 17:45:26 GMT

←Older revision		Revision as of 17:45, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4r7y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638] and [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R7Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R7Y FirstGlance]. <br>		<table><tr><td colspan='2'>[[4r7y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638] and [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R7Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R7Y FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r7y OCA], [https://pdbe.org/4r7y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r7y RCSB], [https://www.ebi.ac.uk/pdbsum/4r7y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r7y ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r7y OCA], [https://pdbe.org/4r7y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r7y RCSB], [https://www.ebi.ac.uk/pdbsum/4r7y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r7y ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:43, 2 March 2023

OCA — Thu, 02 Mar 2023 06:43:42 GMT

←Older revision		Revision as of 06:43, 2 March 2023
Line 1:		Line 1:

	==Crystal structure of an active MCM hexamer==		==Crystal structure of an active MCM hexamer==
-	<StructureSection load='4r7y' size='340' side='right' caption='[[4r7y]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='4r7y' size='340' side='right'caption='[[4r7y]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4r7y]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pyrfu Pyrfu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R7Y OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4R7Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4r7y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638] and [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R7Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R7Y FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4r7z\|4r7z]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r7y OCA], [https://pdbe.org/4r7y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r7y RCSB], [https://www.ebi.ac.uk/pdbsum/4r7y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r7y ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MCM, SSO0774, PF0482 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186497 PYRFU])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4r7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r7y OCA], [~~http~~://pdbe.org/4r7y PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4r7y RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4r7y PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4r7y ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~MCM_SULSO MCM_SULSO~~]] Presumptive replicative helicase. Has ATPase and DNA helicase activities. The latter preferentially melts 5'-tailed oligonucleotides and is stimulated by the SSB protein (single-stranded DNA binding protein). The active ATPase sites in the MCM ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The helicase function is proposed to use a partially sequential mode of ATP hydrolysis; the complex appears to tolerate multiple catalytically inactive subunits.<ref>PMID:11821426</ref>	+	[https://www.uniprot.org/uniprot/Q8U3I4_PYRFU Q8U3I4_PYRFU] [https://www.uniprot.org/uniprot/MCM_SACS2 MCM_SACS2] Presumptive replicative helicase. Has ATPase and DNA helicase activities. The latter preferentially melts 5'-tailed oligonucleotides and is stimulated by the SSB protein (single-stranded DNA binding protein). The active ATPase sites in the MCM ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The helicase function is proposed to use a partially sequential mode of ATP hydrolysis; the complex appears to tolerate multiple catalytically inactive subunits.<ref>PMID:11821426</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~DNA helicase~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Pyrfu~~]]	+	[[Category: Pyrococcus furiosus DSM 3638]]
-	[[Category: ~~Arachea, B T~~]]	+	[[Category: Saccharolobus solfataricus P2]]
-	[[Category: ~~Enemark, E J~~]]	+	[[Category: Arachea BT]]
-	[[Category: ~~Epling, L B~~]]	+	[[Category: Enemark EJ]]
-	[[Category: ~~Miller, J M~~]]	+	[[Category: Epling LB]]
-	[[Category: ~~Aaa+]]~~	+	[[Category: Miller JM]]
-	~~[[Category: Atpase]]~~	+
-	~~[[Category: Dna replication]]~~	+
-	~~[[Category: Helicase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Mcm]]~~	+
-	~~[[Category: Ob-fold~~]]	+

OCA at 02:56, 16 November 2017

OCA — Thu, 16 Nov 2017 02:56:23 GMT

←Older revision		Revision as of 02:56, 16 November 2017
Line 3:		Line 3:
	<StructureSection load='4r7y' size='340' side='right' caption='[[4r7y]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='4r7y' size='340' side='right' caption='[[4r7y]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4r7y]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R7Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R7Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4r7y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrfu Pyrfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R7Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R7Y FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4r7z\|4r7z]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4r7z\|4r7z]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MCM, SSO0774, PF0482 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186497 PYRFU])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r7y OCA], [http://pdbe.org/4r7y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r7y RCSB], [http://www.ebi.ac.uk/pdbsum/4r7y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r7y ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r7y OCA], [http://pdbe.org/4r7y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r7y RCSB], [http://www.ebi.ac.uk/pdbsum/4r7y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r7y ProSAT]</span></td></tr>
Line 25:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: DNA helicase]]		[[Category: DNA helicase]]
		+	[[Category: Pyrfu]]
	[[Category: Arachea, B T]]		[[Category: Arachea, B T]]
	[[Category: Enemark, E J]]		[[Category: Enemark, E J]]

OCA at 15:52, 4 August 2016

OCA — Thu, 04 Aug 2016 15:52:32 GMT

←Older revision		Revision as of 15:52, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of an active MCM hexamer==		==Crystal structure of an active MCM hexamer==
	<StructureSection load='4r7y' size='340' side='right' caption='[[4r7y]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='4r7y' size='340' side='right' caption='[[4r7y]], [[Resolution\|resolution]] 2.70Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4r7z\|4r7z]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4r7z\|4r7z]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r7y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r7y RCSB], [http://www.ebi.ac.uk/pdbsum/4r7y PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r7y OCA], [http://pdbe.org/4r7y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r7y RCSB], [http://www.ebi.ac.uk/pdbsum/4r7y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r7y ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4r7y" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:42, 24 December 2014

OCA — Wed, 24 Dec 2014 16:42:17 GMT

←Older revision		Revision as of 16:42, 24 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r7y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r7y RCSB], [http://www.ebi.ac.uk/pdbsum/4r7y PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r7y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r7y RCSB], [http://www.ebi.ac.uk/pdbsum/4r7y PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MCM_SULSO MCM_SULSO]] Presumptive replicative helicase. Has ATPase and DNA helicase activities. The latter preferentially melts 5'-tailed oligonucleotides and is stimulated by the SSB protein (single-stranded DNA binding protein). The active ATPase sites in the MCM ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The helicase function is proposed to use a partially sequential mode of ATP hydrolysis; the complex appears to tolerate multiple catalytically inactive subunits.<ref>PMID:11821426</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:47, 12 November 2014

OCA — Wed, 12 Nov 2014 07:47:25 GMT

←Older revision		Revision as of 07:47, 12 November 2014
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: DNA helicase]]		[[Category: DNA helicase]]
-	[[Category: Arachea, B T.]]	+	[[Category: Arachea, B T]]
-	[[Category: Enemark, E J.]]	+	[[Category: Enemark, E J]]
-	[[Category: Epling, L B.]]	+	[[Category: Epling, L B]]
-	[[Category: Miller, J M.]]	+	[[Category: Miller, J M]]
	[[Category: Aaa+]]		[[Category: Aaa+]]
	[[Category: Atpase]]		[[Category: Atpase]]

OCA at 07:43, 8 October 2014

OCA — Wed, 08 Oct 2014 07:43:43 GMT

←Older revision		Revision as of 07:43, 8 October 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of an active MCM hexamer==
		+	<StructureSection load='4r7y' size='340' side='right' caption='[[4r7y]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4r7y]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R7Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R7Y FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4r7z\|4r7z]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r7y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r7y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r7y RCSB], [http://www.ebi.ac.uk/pdbsum/4r7y PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In a previous Research article (<xref>Froelich et al., 2014</xref>), we suggested an MCM helicase activation mechanism, but were limited in discussing the ATPase domain because it was absent from the crystal structure. Here we present the crystal structure of a nearly full-length MCM hexamer that is helicase-active and thus has all features essential for unwinding DNA. The structure is a chimera of Sulfolobus solfataricus N-terminal domain and Pyrococcus furiosus ATPase domain. We discuss three major findings: 1) a novel conformation for the A-subdomain that could play a role in MCM regulation; 2) interaction of a universally conserved glutamine in the N-terminal Allosteric Communication Loop with the AAA+ domain helix-2-insert (h2i); and 3) a recessed binding pocket for the MCM ssDNA-binding motif influenced by the h2i. We suggest that during helicase activation, the h2i clamps down on the leading strand to facilitate strand retention and regulate ATP hydrolysis.

-	~~The entry 4r7y is ON HOLD until Paper Publication~~	+	Analysis of the crystal structure of an active MCM hexamer.,Miller JM, Arachea BT, Epling LB, Enemark EJ Elife. 2014 Sep 29;3. doi: 10.7554/eLife.03433. PMID:25262915<ref>PMID:25262915</ref>

-	~~Authors: Miller~~, J.M., Arachea, B.T., Epling, L.B., ~~Enemark, E.~~J.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of an active MCM hexamer~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: DNA helicase]]
		+	[[Category: Arachea, B T.]]
		+	[[Category: Enemark, E J.]]
		+	[[Category: Epling, L B.]]
		+	[[Category: Miller, J M.]]
		+	[[Category: Aaa+]]
		+	[[Category: Atpase]]
		+	[[Category: Dna replication]]
		+	[[Category: Helicase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Mcm]]
		+	[[Category: Ob-fold]]

OCA at 08:24, 24 September 2014

OCA — Wed, 24 Sep 2014 08:24:58 GMT

←Older revision		Revision as of 08:24, 24 September 2014
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4r7y is ON HOLD	+	The entry 4r7y is ON HOLD until Paper Publication

	Authors: Miller, J.M., Arachea, B.T., Epling, L.B., Enemark, E.J.		Authors: Miller, J.M., Arachea, B.T., Epling, L.B., Enemark, E.J.

	Description: Crystal structure of an active MCM hexamer		Description: Crystal structure of an active MCM hexamer

OCA: Protected "4r7y" [edit=sysop:move=sysop]

OCA — Thu, 04 Sep 2014 04:20:01 GMT

Protected "4r7y" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:20, 4 September 2014

OCA: New page: '''Unreleased structure''' The entry 4r7y is ON HOLD Authors: Miller, J.M., Arachea, B.T., Epling, L.B., Enemark, E.J. Description: Crystal structure of an active MCM hexamer

OCA — Thu, 04 Sep 2014 04:19:59 GMT

New page: '''Unreleased structure''' The entry 4r7y is ON HOLD Authors: Miller, J.M., Arachea, B.T., Epling, L.B., Enemark, E.J. Description: Crystal structure of an active MCM hexamer

New page

'''Unreleased structure'''

The entry 4r7y is ON HOLD

Authors: Miller, J.M., Arachea, B.T., Epling, L.B., Enemark, E.J.

Description: Crystal structure of an active MCM hexamer