4rh4 - Revision history

OCA at 12:52, 1 March 2024

OCA — Fri, 01 Mar 2024 12:52:28 GMT

←Older revision		Revision as of 12:52, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4rh4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RH4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RH4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4rh4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RH4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RH4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rh4 OCA], [https://pdbe.org/4rh4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rh4 RCSB], [https://www.ebi.ac.uk/pdbsum/4rh4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rh4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rh4 OCA], [https://pdbe.org/4rh4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rh4 RCSB], [https://www.ebi.ac.uk/pdbsum/4rh4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rh4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/AZUP_ALCFA AZUP_ALCFA] This soluble electron transfer copper protein is required for the inactivation of copper-containing nitrite reductase in the presence of oxygen. Serves as a direct electron donor to the nitrite reductase.		[https://www.uniprot.org/uniprot/AZUP_ALCFA AZUP_ALCFA] This soluble electron transfer copper protein is required for the inactivation of copper-containing nitrite reductase in the presence of oxygen. Serves as a direct electron donor to the nitrite reductase.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The copper(II) centre of the blue copper protein pseudoazurin from Alcaligenes faecalis has been substituted by zinc(II) via denaturing the protein, chelation and removal of copper and refolding the apoprotein, followed by the addition of an aqueous solution of ZnCl2. Vapour-diffusion experiments produced colourless hexagonal crystals (space group P65), which when cryocooled had unit-cell parameters a = b = 49.01, c = 98.08 A. Diffraction data collected at 100 K using a copper sealed tube were phased by the weak anomalous signal of five S atoms and one Zn atom. The structure was fitted manually and refined to 1.6 A resolution. The zinc-substituted protein exhibits similar overall geometry to the native structure with copper. Zn(2+) binds more strongly to its four ligand atoms (His40 N(delta1), Cys78 S(gamma), His81 N(delta1) and Met86 S(delta)) and retains the tetrahedral arrangement, although the structure is less distorted than the native copper protein.
-
-	Zinc-substituted pseudoazurin solved by S/Zn-SAD phasing.,Gessmann R, Papadovasilaki M, Drougkas E, Petratos K Acta Crystallogr F Struct Biol Commun. 2015 Jan 1;71(Pt 1):19-23. doi:, 10.1107/S2053230X14025552. Epub 2015 Jan 1. PMID:25615962<ref>PMID:25615962</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4rh4" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Pseudoazurin\|Pseudoazurin]]		*[[Pseudoazurin\|Pseudoazurin]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:56, 2 March 2023

OCA — Thu, 02 Mar 2023 06:56:22 GMT

←Older revision		Revision as of 06:56, 2 March 2023
Line 3:		Line 3:
	<StructureSection load='4rh4' size='340' side='right'caption='[[4rh4]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='4rh4' size='340' side='right'caption='[[4rh4]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4rh4]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Alcfa Alcfa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RH4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4RH4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4rh4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RH4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RH4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1paz\|1paz]], [[3nyk\|3nyk]], [[1pza\|1pza]], [[1pzc\|1pzc]]~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rh4 OCA], [https://pdbe.org/4rh4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rh4 RCSB], [https://www.ebi.ac.uk/pdbsum/4rh4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rh4 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4rh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rh4 OCA], [~~http~~://pdbe.org/4rh4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4rh4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4rh4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4rh4 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/AZUP_ALCFA AZUP_ALCFA]] This soluble electron transfer copper protein is required for the inactivation of copper-containing nitrite reductase in the presence of oxygen. Serves as a direct electron donor to the nitrite reductase.	+	[https://www.uniprot.org/uniprot/AZUP_ALCFA AZUP_ALCFA] This soluble electron transfer copper protein is required for the inactivation of copper-containing nitrite reductase in the presence of oxygen. Serves as a direct electron donor to the nitrite reductase.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Alcfa~~]]	+	[[Category: Alcaligenes faecalis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gessmann, R]]	+	[[Category: Gessmann R]]
-	[[Category: Petratos, K]]	+	[[Category: Petratos K]]
-	~~[[Category: Beta-sandwich]]~~	+
-	~~[[Category: Divalent metal-ion]]~~	+
-	~~[[Category: Metal binding protein]]~~	+
-	~~[[Category: Metalloprotein]]~~	+
-	~~[[Category: Sad~~]]	+

OCA at 09:56, 1 May 2019

OCA — Wed, 01 May 2019 09:56:26 GMT

←Older revision		Revision as of 09:56, 1 May 2019
Line 1:		Line 1:

	==Zinc-substituted pseudoazurin solved by S/Zn-SAD phasing==		==Zinc-substituted pseudoazurin solved by S/Zn-SAD phasing==
-	<StructureSection load='4rh4' size='340' side='right' caption='[[4rh4]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='4rh4' size='340' side='right'caption='[[4rh4]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4rh4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RH4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RH4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4rh4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Alcfa Alcfa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RH4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RH4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1paz\|1paz]], [[3nyk\|3nyk]], [[1pza\|1pza]], [[1pzc\|1pzc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1paz\|1paz]], [[3nyk\|3nyk]], [[1pza\|1pza]], [[1pzc\|1pzc]]</td></tr>
Line 26:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Alcfa]]
		+	[[Category: Large Structures]]
	[[Category: Gessmann, R]]		[[Category: Gessmann, R]]
	[[Category: Petratos, K]]		[[Category: Petratos, K]]

OCA at 10:56, 11 August 2016

OCA — Thu, 11 Aug 2016 10:56:36 GMT

←Older revision		Revision as of 10:56, 11 August 2016
Line 1:		Line 1:
		+
	==Zinc-substituted pseudoazurin solved by S/Zn-SAD phasing==		==Zinc-substituted pseudoazurin solved by S/Zn-SAD phasing==
	<StructureSection load='4rh4' size='340' side='right' caption='[[4rh4]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='4rh4' size='340' side='right' caption='[[4rh4]], [[Resolution\|resolution]] 1.60Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1paz\|1paz]], [[3nyk\|3nyk]], [[1pza\|1pza]], [[1pzc\|1pzc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1paz\|1paz]], [[3nyk\|3nyk]], [[1pza\|1pza]], [[1pzc\|1pzc]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rh4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rh4 RCSB], [http://www.ebi.ac.uk/pdbsum/4rh4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rh4 OCA], [http://pdbe.org/4rh4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rh4 RCSB], [http://www.ebi.ac.uk/pdbsum/4rh4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rh4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4rh4" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Pseudoazurin\|Pseudoazurin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:15, 7 February 2015

OCA — Sat, 07 Feb 2015 10:15:50 GMT

←Older revision		Revision as of 10:15, 7 February 2015
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/AZUP_ALCFA AZUP_ALCFA]] This soluble electron transfer copper protein is required for the inactivation of copper-containing nitrite reductase in the presence of oxygen. Serves as a direct electron donor to the nitrite reductase.		[[http://www.uniprot.org/uniprot/AZUP_ALCFA AZUP_ALCFA]] This soluble electron transfer copper protein is required for the inactivation of copper-containing nitrite reductase in the presence of oxygen. Serves as a direct electron donor to the nitrite reductase.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The copper(II) centre of the blue copper protein pseudoazurin from Alcaligenes faecalis has been substituted by zinc(II) via denaturing the protein, chelation and removal of copper and refolding the apoprotein, followed by the addition of an aqueous solution of ZnCl2. Vapour-diffusion experiments produced colourless hexagonal crystals (space group P65), which when cryocooled had unit-cell parameters a = b = 49.01, c = 98.08 A. Diffraction data collected at 100 K using a copper sealed tube were phased by the weak anomalous signal of five S atoms and one Zn atom. The structure was fitted manually and refined to 1.6 A resolution. The zinc-substituted protein exhibits similar overall geometry to the native structure with copper. Zn(2+) binds more strongly to its four ligand atoms (His40 N(delta1), Cys78 S(gamma), His81 N(delta1) and Met86 S(delta)) and retains the tetrahedral arrangement, although the structure is less distorted than the native copper protein.
		+
		+	Zinc-substituted pseudoazurin solved by S/Zn-SAD phasing.,Gessmann R, Papadovasilaki M, Drougkas E, Petratos K Acta Crystallogr F Struct Biol Commun. 2015 Jan 1;71(Pt 1):19-23. doi:, 10.1107/S2053230X14025552. Epub 2015 Jan 1. PMID:25615962<ref>PMID:25615962</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 13:44, 14 January 2015

OCA — Wed, 14 Jan 2015 13:44:21 GMT

←Older revision		Revision as of 13:44, 14 January 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Zinc-substituted pseudoazurin solved by S/Zn-SAD phasing==
-		+	<StructureSection load='4rh4' size='340' side='right' caption='[[4rh4]], [[Resolution\|resolution]] 1.60Å' scene=''>
-	~~The entry~~ 4rh4 is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4rh4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RH4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RH4 FirstGlance]. <br>
-	~~Authors~~: ~~Gessmann~~, R., ~~Petratos~~, K.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1paz\|1paz]], [[3nyk\|3nyk]], [[1pza\|1pza]], [[1pzc\|1pzc]]</td></tr>
-	~~Description~~: ~~Zinc~~-~~substituted Pseudoazurin solved by S~~/Zn-~~SAD method~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rh4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rh4 RCSB], [http://www.ebi.ac.uk/pdbsum/4rh4 PDBsum]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/AZUP_ALCFA AZUP_ALCFA]] This soluble electron transfer copper protein is required for the inactivation of copper-containing nitrite reductase in the presence of oxygen. Serves as a direct electron donor to the nitrite reductase.
		+	__TOC__
		+	</StructureSection>
	[[Category: Gessmann, R]]		[[Category: Gessmann, R]]
	[[Category: Petratos, K]]		[[Category: Petratos, K]]
		+	[[Category: Beta-sandwich]]
		+	[[Category: Divalent metal-ion]]
		+	[[Category: Metal binding protein]]
		+	[[Category: Metalloprotein]]
		+	[[Category: Sad]]

OCA at 16:52, 24 December 2014

OCA — Wed, 24 Dec 2014 16:52:17 GMT

←Older revision		Revision as of 16:52, 24 December 2014
Line 6:		Line 6:

	Description: Zinc-substituted Pseudoazurin solved by S/Zn-SAD method		Description: Zinc-substituted Pseudoazurin solved by S/Zn-SAD method
		+	[[Category: Unreleased Structures]]
		+	[[Category: Gessmann, R]]
		+	[[Category: Petratos, K]]

OCA at 10:06, 29 October 2014

OCA — Wed, 29 Oct 2014 10:06:15 GMT

←Older revision		Revision as of 10:06, 29 October 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4rh4 is ON HOLD	+	The entry 4rh4 is ON HOLD until Paper Publication

	Authors: Gessmann, R., Petratos, K.		Authors: Gessmann, R., Petratos, K.

-	Description: Zinc-substituted Pseudoazurin solved by SAD method	+	Description: Zinc-substituted Pseudoazurin solved by S/Zn-SAD method

OCA at 09:28, 20 October 2014

OCA — Mon, 20 Oct 2014 09:28:15 GMT

←Older revision		Revision as of 09:28, 20 October 2014
Line 5:		Line 5:
	Authors: Gessmann, R., Petratos, K.		Authors: Gessmann, R., Petratos, K.

-	Description: ~~ZINC~~-~~SUBSTITUTED PSEUDOAZURIN~~ solved by SAD method	+	Description: Zinc-substituted Pseudoazurin solved by SAD method

OCA: Protected "4rh4" [edit=sysop:move=sysop]

OCA — Wed, 08 Oct 2014 06:35:09 GMT

Protected "4rh4" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:35, 8 October 2014