4rhw - Revision history

OCA at 15:15, 8 November 2023

OCA — Wed, 08 Nov 2023 15:15:39 GMT

←Older revision		Revision as of 15:15, 8 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4rhw]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RHW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RHW FirstGlance]. <br>		<table><tr><td colspan='2'>[[4rhw]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RHW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RHW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rhw OCA], [https://pdbe.org/4rhw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rhw RCSB], [https://www.ebi.ac.uk/pdbsum/4rhw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rhw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rhw OCA], [https://pdbe.org/4rhw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rhw RCSB], [https://www.ebi.ac.uk/pdbsum/4rhw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rhw ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:57, 2 March 2023

OCA — Thu, 02 Mar 2023 06:57:25 GMT

←Older revision		Revision as of 06:57, 2 March 2023
Line 1:		Line 1:

	==Crystal structure of Apaf-1 CARD and caspase-9 CARD complex==		==Crystal structure of Apaf-1 CARD and caspase-9 CARD complex==
-	<StructureSection load='4rhw' size='340' side='right' caption='[[4rhw]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='4rhw' size='340' side='right'caption='[[4rhw]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4rhw]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RHW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4RHW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4rhw]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RHW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RHW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">APAF1, KIAA0413 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CASP9, MCH6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rhw OCA], [https://pdbe.org/4rhw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rhw RCSB], [https://www.ebi.ac.uk/pdbsum/4rhw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rhw ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Caspase-9 Caspase-9], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.62 3.4.22.62] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4rhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rhw OCA], [~~http~~://pdbe.org/4rhw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4rhw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4rhw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4rhw ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/APAF_HUMAN APAF_HUMAN]] Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.<ref>PMID:10393175</ref> <ref>PMID:12804598</ref> [[http://www.uniprot.org/uniprot/CASP9_HUMAN CASP9_HUMAN]] Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).<ref>PMID:15657060</ref> Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.<ref>PMID:15657060</ref>	+	[https://www.uniprot.org/uniprot/APAF_HUMAN APAF_HUMAN] Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.<ref>PMID:10393175</ref> <ref>PMID:12804598</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 20:

	==See Also==		==See Also==
-	*[[Apoptotic protease-activating factor\|Apoptotic protease-activating factor]]	+	*[[Apoptotic protease-activating factor-1 3D structures\|Apoptotic protease-activating factor-1 3D structures]]
-	*[[Caspase\|Caspase]]	+	*[[Caspase 3D structures\|Caspase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Caspase-9~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Human~~]]	+	[[Category: Large Structures]]
-	[[Category: Hu, Q]]	+	[[Category: Hu Q]]
-	[[Category: Shi, Y]]	+	[[Category: Shi Y]]
-	[[Category: Wu, D]]	+	[[Category: Wu D]]
-	[[Category: Yan, C]]	+	[[Category: Yan C]]
-	~~[[Category: Apoptosis]]~~	+
-	~~[[Category: Death domain superfamily~~]]	+

OCA at 08:18, 17 October 2018

OCA — Wed, 17 Oct 2018 08:18:49 GMT

←Older revision		Revision as of 08:18, 17 October 2018
Line 3:		Line 3:
	<StructureSection load='4rhw' size='340' side='right' caption='[[4rhw]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='4rhw' size='340' side='right' caption='[[4rhw]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4rhw]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RHW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RHW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4rhw]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RHW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RHW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">APAF1, KIAA0413 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CASP9, MCH6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Caspase-9 Caspase-9], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.62 3.4.22.62] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Caspase-9 Caspase-9], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.62 3.4.22.62] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rhw OCA], [http://pdbe.org/4rhw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rhw RCSB], [http://www.ebi.ac.uk/pdbsum/4rhw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rhw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rhw OCA], [http://pdbe.org/4rhw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rhw RCSB], [http://www.ebi.ac.uk/pdbsum/4rhw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rhw ProSAT]</span></td></tr>
Line 10:		Line 11:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/APAF_HUMAN APAF_HUMAN]] Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.<ref>PMID:10393175</ref> <ref>PMID:12804598</ref> [[http://www.uniprot.org/uniprot/CASP9_HUMAN CASP9_HUMAN]] Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).<ref>PMID:15657060</ref> Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.<ref>PMID:15657060</ref>		[[http://www.uniprot.org/uniprot/APAF_HUMAN APAF_HUMAN]] Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.<ref>PMID:10393175</ref> <ref>PMID:12804598</ref> [[http://www.uniprot.org/uniprot/CASP9_HUMAN CASP9_HUMAN]] Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).<ref>PMID:15657060</ref> Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.<ref>PMID:15657060</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Autocatalytic activation of an initiator caspase triggers the onset of apoptosis. In dying cells, caspase-9 activation is mediated by a multimeric adaptor complex known as the Apaf-1 apoptosome. The molecular mechanism by which caspase-9 is activated by the Apaf-1 apoptosome remains largely unknown. Here we demonstrate that the previously reported 1:1 interaction between Apaf-1 caspase recruitment domain (CARD) and caspase-9 CARD is insufficient for the activation of caspase-9. Rather, formation of a multimeric CARD:CARD assembly between Apaf-1 and caspase-9, which requires three types of distinct interfaces, underlies caspase-9 activation. Importantly, an additional surface area on the multimeric CARD assembly is essential for caspase-9 activation. Together, these findings reveal mechanistic insights into the activation of caspase-9 by the Apaf-1 apoptosome and support the induced conformation model for initiator caspase activation by adaptor complexes.
		+
		+	Molecular determinants of caspase-9 activation by the Apaf-1 apoptosome.,Hu Q, Wu D, Chen W, Yan Z, Yan C, He T, Liang Q, Shi Y Proc Natl Acad Sci U S A. 2014 Nov 18;111(46):16254-61. doi:, 10.1073/pnas.1418000111. Epub 2014 Oct 13. PMID:25313070<ref>PMID:25313070</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4rhw" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
		+	*[[Apoptotic protease-activating factor\|Apoptotic protease-activating factor]]
	*[[Caspase\|Caspase]]		*[[Caspase\|Caspase]]
	== References ==		== References ==
Line 18:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Caspase-9]]		[[Category: Caspase-9]]
		+	[[Category: Human]]
	[[Category: Hu, Q]]		[[Category: Hu, Q]]
	[[Category: Shi, Y]]		[[Category: Shi, Y]]

OCA at 04:15, 5 August 2016

OCA — Fri, 05 Aug 2016 04:15:58 GMT

←Older revision		Revision as of 04:15, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Apaf-1 CARD and caspase-9 CARD complex==		==Crystal structure of Apaf-1 CARD and caspase-9 CARD complex==
	<StructureSection load='4rhw' size='340' side='right' caption='[[4rhw]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='4rhw' size='340' side='right' caption='[[4rhw]], [[Resolution\|resolution]] 2.10Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Caspase-9 Caspase-9], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.62 3.4.22.62] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Caspase-9 Caspase-9], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.62 3.4.22.62] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rhw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rhw RCSB], [http://www.ebi.ac.uk/pdbsum/4rhw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rhw OCA], [http://pdbe.org/4rhw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rhw RCSB], [http://www.ebi.ac.uk/pdbsum/4rhw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rhw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/APAF_HUMAN APAF_HUMAN]] Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.<ref>PMID:10393175</ref> <ref>PMID:12804598</ref> [[http://www.uniprot.org/uniprot/CASP9_HUMAN CASP9_HUMAN]] Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).<ref>PMID:15657060</ref> Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.<ref>PMID:15657060</ref>		[[http://www.uniprot.org/uniprot/APAF_HUMAN APAF_HUMAN]] Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.<ref>PMID:10393175</ref> <ref>PMID:12804598</ref> [[http://www.uniprot.org/uniprot/CASP9_HUMAN CASP9_HUMAN]] Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).<ref>PMID:15657060</ref> Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.<ref>PMID:15657060</ref>
		+
		+	==See Also==
		+	*[[Caspase\|Caspase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 15:45, 25 December 2014

OCA — Thu, 25 Dec 2014 15:45:29 GMT

←Older revision		Revision as of 15:45, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rhw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rhw RCSB], [http://www.ebi.ac.uk/pdbsum/4rhw PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rhw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rhw RCSB], [http://www.ebi.ac.uk/pdbsum/4rhw PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/APAF_HUMAN APAF_HUMAN]] Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.<ref>PMID:10393175</ref> <ref>PMID:12804598</ref> [[http://www.uniprot.org/uniprot/CASP9_HUMAN CASP9_HUMAN]] Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).<ref>PMID:15657060</ref> Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.<ref>PMID:15657060</ref>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Caspase-9]]		[[Category: Caspase-9]]
-	[[Category: Hu, Q.]]	+	[[Category: Hu, Q]]
-	[[Category: Shi, Y.]]	+	[[Category: Shi, Y]]
-	[[Category: Wu, D.]]	+	[[Category: Wu, D]]
-	[[Category: Yan, C.]]	+	[[Category: Yan, C]]
	[[Category: Apoptosis]]		[[Category: Apoptosis]]
	[[Category: Death domain superfamily]]		[[Category: Death domain superfamily]]

OCA at 11:04, 20 October 2014

OCA — Mon, 20 Oct 2014 11:04:08 GMT

←Older revision		Revision as of 11:04, 20 October 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal structure of Apaf-1 CARD and caspase-9 CARD complex==
-		+	<StructureSection load='4rhw' size='340' side='right' caption='[[4rhw]], [[Resolution\|resolution]] 2.10Å' scene=''>
-	~~The entry~~ 4rhw is ~~ON HOLD~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4rhw]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RHW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RHW FirstGlance]. <br>
-	~~Authors~~: Hu, Q., Wu, D., Yan, C.~~, Shi, Y.~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Caspase-9 Caspase-9], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.62 3.4.22.62] </span></td></tr>
-	~~Description~~: ~~Crystal structure of Apaf-1 CARD and caspase-9 CARD complex~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rhw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rhw RCSB], [http://www.ebi.ac.uk/pdbsum/4rhw PDBsum]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Caspase-9]]
		+	[[Category: Hu, Q.]]
		+	[[Category: Shi, Y.]]
		+	[[Category: Wu, D.]]
		+	[[Category: Yan, C.]]
		+	[[Category: Apoptosis]]
		+	[[Category: Death domain superfamily]]

OCA: Protected "4rhw" [edit=sysop:move=sysop]

OCA — Wed, 08 Oct 2014 06:36:15 GMT

Protected "4rhw" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:36, 8 October 2014

OCA: New page: '''Unreleased structure''' The entry 4rhw is ON HOLD Authors: Hu, Q., Wu, D., Yan, C., Shi, Y. Description: Crystal structure of Apaf-1 CARD and caspase-9 CARD complex

OCA — Wed, 08 Oct 2014 06:36:13 GMT

New page: '''Unreleased structure''' The entry 4rhw is ON HOLD Authors: Hu, Q., Wu, D., Yan, C., Shi, Y. Description: Crystal structure of Apaf-1 CARD and caspase-9 CARD complex

New page

'''Unreleased structure'''

The entry 4rhw is ON HOLD

Authors: Hu, Q., Wu, D., Yan, C., Shi, Y.

Description: Crystal structure of Apaf-1 CARD and caspase-9 CARD complex