4rjt - Revision history

OCA at 17:50, 20 September 2023

OCA — Wed, 20 Sep 2023 17:50:58 GMT

←Older revision		Revision as of 17:50, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4rjt]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RJT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RJT FirstGlance]. <br>		<table><tr><td colspan='2'>[[4rjt]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RJT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RJT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rjt OCA], [https://pdbe.org/4rjt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rjt RCSB], [https://www.ebi.ac.uk/pdbsum/4rjt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rjt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rjt OCA], [https://pdbe.org/4rjt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rjt RCSB], [https://www.ebi.ac.uk/pdbsum/4rjt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rjt ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:59, 2 March 2023

OCA — Thu, 02 Mar 2023 06:59:36 GMT

←Older revision		Revision as of 06:59, 2 March 2023
Line 1:		Line 1:

	==Crystal Structure of Unliganded, Full Length hUGDH at pH 7.0==		==Crystal Structure of Unliganded, Full Length hUGDH at pH 7.0==
-	<StructureSection load='4rjt' size='340' side='right' caption='[[4rjt]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='4rjt' size='340' side='right'caption='[[4rjt]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4rjt]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RJT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4RJT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4rjt]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RJT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RJT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qej\|4qej]], [[3prj\|3prj]], [[3ptz\|3ptz]], [[3tf5\|3tf5]], [[2q3e\|2q3e]], [[2qg4\|2qg4]]</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rjt OCA], [https://pdbe.org/4rjt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rjt RCSB], [https://www.ebi.ac.uk/pdbsum/4rjt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rjt ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">UGDH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-glucose_6-dehydrogenase UDP-glucose 6-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.22 1.1.1.22] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4rjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rjt OCA], [~~http~~://pdbe.org/4rjt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4rjt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4rjt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4rjt ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/UGDH_HUMAN UGDH_HUMAN]] Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.	+	[https://www.uniprot.org/uniprot/UGDH_HUMAN UGDH_HUMAN] Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~UDP-glucose 6-dehydrogenase~~]]	+	[[Category: Large Structures]]
-	[[Category: Sidlo~~, A M~~]]	+	[[Category: Sidlo AM]]
-	[[Category: Wood~~, Z A]]~~	+	[[Category: Wood ZA]]
-	~~[[Category: Cytosol]]~~	+
-	~~[[Category: Nad binding]]~~	+
-	~~[[Category: Nucleotide sugar dehydrogenase]]~~	+
-	~~[[Category: Oxidation]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Rossmann fold]]~~	+
-	~~[[Category: Udp-glucose binding~~]]	+

OCA at 05:45, 25 April 2018

OCA — Wed, 25 Apr 2018 05:45:02 GMT

←Older revision		Revision as of 05:45, 25 April 2018
Line 3:		Line 3:
	<StructureSection load='4rjt' size='340' side='right' caption='[[4rjt]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='4rjt' size='340' side='right' caption='[[4rjt]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4rjt]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RJT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4rjt]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RJT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qej\|4qej]], [[3prj\|3prj]], [[3ptz\|3ptz]], [[3tf5\|3tf5]], [[2q3e\|2q3e]], [[2qg4\|2qg4]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qej\|4qej]], [[3prj\|3prj]], [[3ptz\|3ptz]], [[3tf5\|3tf5]], [[2q3e\|2q3e]], [[2qg4\|2qg4]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">UGDH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-glucose_6-dehydrogenase UDP-glucose 6-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.22 1.1.1.22] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-glucose_6-dehydrogenase UDP-glucose 6-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.22 1.1.1.22] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rjt OCA], [http://pdbe.org/4rjt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rjt RCSB], [http://www.ebi.ac.uk/pdbsum/4rjt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rjt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rjt OCA], [http://pdbe.org/4rjt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rjt RCSB], [http://www.ebi.ac.uk/pdbsum/4rjt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rjt ProSAT]</span></td></tr>
Line 24:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: UDP-glucose 6-dehydrogenase]]		[[Category: UDP-glucose 6-dehydrogenase]]
	[[Category: Sidlo, A M]]		[[Category: Sidlo, A M]]

OCA at 07:59, 11 August 2016

OCA — Thu, 11 Aug 2016 07:59:38 GMT

←Older revision		Revision as of 07:59, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of Unliganded, Full Length hUGDH at pH 7.0==		==Crystal Structure of Unliganded, Full Length hUGDH at pH 7.0==
	<StructureSection load='4rjt' size='340' side='right' caption='[[4rjt]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='4rjt' size='340' side='right' caption='[[4rjt]], [[Resolution\|resolution]] 2.70Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qej\|4qej]], [[3prj\|3prj]], [[3ptz\|3ptz]], [[3tf5\|3tf5]], [[2q3e\|2q3e]], [[2qg4\|2qg4]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qej\|4qej]], [[3prj\|3prj]], [[3ptz\|3ptz]], [[3tf5\|3tf5]], [[2q3e\|2q3e]], [[2qg4\|2qg4]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-glucose_6-dehydrogenase UDP-glucose 6-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.22 1.1.1.22] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-glucose_6-dehydrogenase UDP-glucose 6-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.22 1.1.1.22] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rjt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rjt RCSB], [http://www.ebi.ac.uk/pdbsum/4rjt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rjt OCA], [http://pdbe.org/4rjt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rjt RCSB], [http://www.ebi.ac.uk/pdbsum/4rjt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rjt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4rjt" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:48, 31 December 2014

OCA — Wed, 31 Dec 2014 14:48:38 GMT

←Older revision		Revision as of 14:48, 31 December 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal Structure of Unliganded, Full Length hUGDH at pH 7.0==
		+	<StructureSection load='4rjt' size='340' side='right' caption='[[4rjt]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4rjt]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RJT FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qej\|4qej]], [[3prj\|3prj]], [[3ptz\|3ptz]], [[3tf5\|3tf5]], [[2q3e\|2q3e]], [[2qg4\|2qg4]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/UDP-glucose_6-dehydrogenase UDP-glucose 6-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.22 1.1.1.22] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rjt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rjt RCSB], [http://www.ebi.ac.uk/pdbsum/4rjt PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/UGDH_HUMAN UGDH_HUMAN]] Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Human UDP-alpha-d-glucose-6-dehydrogenase (hUGDH) displays hysteresis because of a slow isomerization from an inactive state (E) to an active state (E). Here we show that the structure of E constrains hUGDH in a conformation that favors feedback inhibition at physiological pH. The feedback inhibitor UDP-alpha-d-xylose (UDP-Xyl) competes with the substrate UDP-alpha-d-glucose for the active site. Upon binding, UDP-Xyl triggers an allosteric switch that changes the structure and affinity of the intersubunit interface to form a stable but inactive horseshoe-shaped hexamer. Using sedimentation velocity studies and a new crystal structure, we show that E* represents a stable conformational intermediate between the active and feedback-inhibited conformations. Because the allosteric switch occludes the cofactor and substrate binding sites in the inactive hexamer, the intermediate conformation observed in the crystal structure is consistent with the E* transient observed in relaxation studies. Steady-state analysis shows that the E* conformation enhances the affinity of hUGDH for the allosteric inhibitor UDP-Xyl by 8.6-fold (Ki = 810 nM). We present a model in which the constrained quaternary structure permits a small effector molecule to leverage a disproportionately large allosteric response.

-	~~The entry 4rjt is ON HOLD~~	+	Hysteresis in Human UDP-Glucose Dehydrogenase Is Due to a Restrained Hexameric Structure That Favors Feedback Inhibition.,Kadirvelraj R, Custer GS, Keul ND, Sennett NC, Sidlo AM, Walsh RM Jr, Wood ZA Biochemistry. 2014 Dec 30;53(51):8043-51. doi: 10.1021/bi500594x. Epub 2014 Dec, 18. PMID:25478983<ref>PMID:25478983</ref>

-	~~Authors: Sidlo~~, A.M.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Crystal Structure~~ of ~~Unliganded, Full Length hUGDH at pH 7~~.0	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
-	[[Category: Sidlo, A.M]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: UDP-glucose 6-dehydrogenase]]
		+	[[Category: Sidlo, A M]]
		+	[[Category: Wood, Z A]]
		+	[[Category: Cytosol]]
		+	[[Category: Nad binding]]
		+	[[Category: Nucleotide sugar dehydrogenase]]
		+	[[Category: Oxidation]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Rossmann fold]]
		+	[[Category: Udp-glucose binding]]

OCA at 16:57, 24 December 2014

OCA — Wed, 24 Dec 2014 16:57:54 GMT

←Older revision		Revision as of 16:57, 24 December 2014
Line 6:		Line 6:

	Description: Crystal Structure of Unliganded, Full Length hUGDH at pH 7.0		Description: Crystal Structure of Unliganded, Full Length hUGDH at pH 7.0
		+	[[Category: Unreleased Structures]]
		+	[[Category: Sidlo, A.M]]

OCA: Protected "4rjt" [edit=sysop:move=sysop]

OCA — Mon, 20 Oct 2014 09:32:25 GMT

Protected "4rjt" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:32, 20 October 2014

OCA: New page: '''Unreleased structure''' The entry 4rjt is ON HOLD Authors: Sidlo, A.M. Description: Crystal Structure of Unliganded, Full Length hUGDH at pH 7.0

OCA — Mon, 20 Oct 2014 09:32:23 GMT

New page: '''Unreleased structure''' The entry 4rjt is ON HOLD Authors: Sidlo, A.M. Description: Crystal Structure of Unliganded, Full Length hUGDH at pH 7.0

New page

'''Unreleased structure'''

The entry 4rjt is ON HOLD

Authors: Sidlo, A.M.

Description: Crystal Structure of Unliganded, Full Length hUGDH at pH 7.0