4rm4 - Revision history

OCA at 17:52, 20 September 2023

OCA — Wed, 20 Sep 2023 17:52:03 GMT

←Older revision		Revision as of 17:52, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4rm4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis Bacillus subtilis subsp. subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RM4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RM4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4rm4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis Bacillus subtilis subsp. subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RM4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RM4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.771Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rm4 OCA], [https://pdbe.org/4rm4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rm4 RCSB], [https://www.ebi.ac.uk/pdbsum/4rm4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rm4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rm4 OCA], [https://pdbe.org/4rm4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rm4 RCSB], [https://www.ebi.ac.uk/pdbsum/4rm4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rm4 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:03, 2 March 2023

OCA — Thu, 02 Mar 2023 07:03:18 GMT

←Older revision		Revision as of 07:03, 2 March 2023
Line 3:		Line 3:
	<StructureSection load='4rm4' size='340' side='right'caption='[[4rm4]], [[Resolution\|resolution]] 1.77Å' scene=''>		<StructureSection load='4rm4' size='340' side='right'caption='[[4rm4]], [[Resolution\|resolution]] 1.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4rm4]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis Bacillus subtilis subsp. subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RM4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4RM4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4rm4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis Bacillus subtilis subsp. subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RM4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RM4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU12210, genomic DNA, yjiB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=135461 Bacillus subtilis subsp. subtilis])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rm4 OCA], [https://pdbe.org/4rm4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rm4 RCSB], [https://www.ebi.ac.uk/pdbsum/4rm4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rm4 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4rm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rm4 OCA], [~~http~~://pdbe.org/4rm4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4rm4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4rm4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4rm4 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/YJIB_BACSU YJIB_BACSU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 20:

	==See Also==		==See Also==
-	*[[Cytochrome P450\|Cytochrome P450]]	+	*[[Cytochrome P450 3D structures\|Cytochrome P450 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 27:
	[[Category: Bacillus subtilis subsp. subtilis]]		[[Category: Bacillus subtilis subsp. subtilis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bell~~, S G~~]]	+	[[Category: Bell SG]]
-	[[Category: Cryle~~, M J~~]]	+	[[Category: Cryle MJ]]
-	[[Category: Hall~~, E A~~]]	+	[[Category: Hall EA]]
-	[[Category: Hutchinson, S]]	+	[[Category: Hutchinson S]]
-	[[Category: Wong, L L]]	+	[[Category: Wong L-L]]
-	[[Category: Zhang~~, A L~~]]	+	[[Category: Zhang AL]]
-	[[Category: Zhang, T]]	+	[[Category: Zhang T]]
-	[[Category: Zhouw~~, W H]]~~	+	[[Category: Zhouw WH]]
-	~~[[Category: Catabolism]]~~	+
-	~~[[Category: Cytochrome p450]]~~	+
-	~~[[Category: Electron transport]]~~	+
-	~~[[Category: Secondary metabolites biosynthesis]]~~	+
-	~~[[Category: Transport~~]]	+

OCA at 09:35, 1 May 2019

OCA — Wed, 01 May 2019 09:35:45 GMT

←Older revision		Revision as of 09:35, 1 May 2019
Line 1:		Line 1:

	==The crystal structure of the versatile cytochrome P450 enzyme CYP109B1 from Bacillus subtilis==		==The crystal structure of the versatile cytochrome P450 enzyme CYP109B1 from Bacillus subtilis==
-	<StructureSection load='4rm4' size='340' side='right' caption='[[4rm4]], [[Resolution\|resolution]] 1.77Å' scene=''>	+	<StructureSection load='4rm4' size='340' side='right'caption='[[4rm4]], [[Resolution\|resolution]] 1.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4rm4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RM4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RM4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4rm4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis Bacillus subtilis subsp. subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RM4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RM4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU12210, genomic DNA, yjiB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=135461 Bacillus subtilis subsp. subtilis])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rm4 OCA], [http://pdbe.org/4rm4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rm4 RCSB], [http://www.ebi.ac.uk/pdbsum/4rm4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rm4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rm4 OCA], [http://pdbe.org/4rm4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rm4 RCSB], [http://www.ebi.ac.uk/pdbsum/4rm4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rm4 ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus subtilis subsp. subtilis]]
		+	[[Category: Large Structures]]
	[[Category: Bell, S G]]		[[Category: Bell, S G]]
	[[Category: Cryle, M J]]		[[Category: Cryle, M J]]

OCA at 17:16, 18 January 2017

OCA — Wed, 18 Jan 2017 17:16:27 GMT

←Older revision		Revision as of 17:16, 18 January 2017
Line 1:		Line 1:
		+
	==The crystal structure of the versatile cytochrome P450 enzyme CYP109B1 from Bacillus subtilis==		==The crystal structure of the versatile cytochrome P450 enzyme CYP109B1 from Bacillus subtilis==
	<StructureSection load='4rm4' size='340' side='right' caption='[[4rm4]], [[Resolution\|resolution]] 1.77Å' scene=''>		<StructureSection load='4rm4' size='340' side='right' caption='[[4rm4]], [[Resolution\|resolution]] 1.77Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4rm4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RM4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RM4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4rm4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RM4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RM4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rm4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rm4 RCSB], [http://www.ebi.ac.uk/pdbsum/4rm4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rm4 OCA], [http://pdbe.org/4rm4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rm4 RCSB], [http://www.ebi.ac.uk/pdbsum/4rm4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rm4 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4rm4" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cytochrome P450\|Cytochrome P450]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 15:42, 7 February 2015

OCA — Sat, 07 Feb 2015 15:42:16 GMT

←Older revision		Revision as of 15:42, 7 February 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==The crystal structure of the versatile cytochrome P450 enzyme CYP109B1 from Bacillus subtilis==
		+	<StructureSection load='4rm4' size='340' side='right' caption='[[4rm4]], [[Resolution\|resolution]] 1.77Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4rm4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RM4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RM4 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rm4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rm4 RCSB], [http://www.ebi.ac.uk/pdbsum/4rm4 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structure of the versatile CYP109B1 enzyme from Bacillus subtilis has been solved at 1.8 A resolution. This is the first structure of an enzyme from this CYP family, whose members are prevalent across diverse species of bacteria. In the crystal structure the enzyme has an open conformation with an access channel leading from the heme to the surface. The substrate-free structure reveals the location of the key residues in the active site that are responsible for binding the substrate in the correct orientation for regioselective oxidation. Importantly, there are significant differences among these residues in members of the CYP109 and closely related CYP106 families and these likely account for the variations in substrate binding and oxidation profiles observed with these enzymes. A whole-cell oxidation biosystem was developed, which contains CYP109B1 and a phthalate family oxygenase reductase (PFOR), from Pseudomonas putida KT24440, as the electron transfer partner. This electron transfer system is able to support CYP109B1 activity resulting in the regioselective hydroxylation of both alpha- and beta-ionone in vivo and in vitro. The PFOR is therefore a versatile electron transfer partner that is able to support the activity of CYP enzymes from other bacterium. The crystal structure of CYP109B1 has a positively charged proximal face and this explains why it can interact with PFOR and adrenodoxin which are predominantly negatively charged around their [2Fe-2S] clusters.

-	The ~~entry 4rm4 is ON HOLD until Paper Publication~~	+	The crystal structure of the versatile cytochrome P450 enzyme CYP109B1 from Bacillus subtilis.,Zhang A, Zhang T, Hall EA, Hutchinson S, Cryle MJ, Wong LL, Zhou W, Bell SG Mol Biosyst. 2015 Jan 14. PMID:25587700<ref>PMID:25587700</ref>

-	~~Authors: Zhouw~~, W.~~H., Zhang, A.L., Zhang, T., Hall, E.A., Hutchinson,~~ S.~~, Cryle, M~~.~~J., Wong, L.-L.,~~ Bell, S.G.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~The crystal structure of the versatile cytochrome P450 enzyme CYP109B1 from Bacillus subtilis~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bell, S G]]
		+	[[Category: Cryle, M J]]
		+	[[Category: Hall, E A]]
	[[Category: Hutchinson, S]]		[[Category: Hutchinson, S]]
-	[[Category: ~~Zhang~~, A.L]]	+	[[Category: Wong, L L]]
-	[[Category: ~~Hall~~, E.A]]	+	[[Category: Zhang, A L]]
	[[Category: Zhang, T]]		[[Category: Zhang, T]]
-	[[Category: Zhouw, W.H]]	+	[[Category: Zhouw, W H]]
-	[[Category: ~~Wong, L.-L~~]]	+	[[Category: Catabolism]]
-	[[Category: ~~Bell, S.G~~]]	+	[[Category: Cytochrome p450]]
-	[[Category: ~~Cryle, M.J~~]]	+	[[Category: Electron transport]]
		+	[[Category: Secondary metabolites biosynthesis]]
		+	[[Category: Transport]]

OCA at 11:58, 31 December 2014

OCA — Wed, 31 Dec 2014 11:58:33 GMT

←Older revision		Revision as of 11:58, 31 December 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4rm4 is ON HOLD	+	The entry 4rm4 is ON HOLD until Paper Publication

	Authors: Zhouw, W.H., Zhang, A.L., Zhang, T., Hall, E.A., Hutchinson, S., Cryle, M.J., Wong, L.-L., Bell, S.G.		Authors: Zhouw, W.H., Zhang, A.L., Zhang, T., Hall, E.A., Hutchinson, S., Cryle, M.J., Wong, L.-L., Bell, S.G.

OCA at 17:01, 24 December 2014

OCA — Wed, 24 Dec 2014 17:01:36 GMT

←Older revision		Revision as of 17:01, 24 December 2014
Line 6:		Line 6:

	Description: The crystal structure of the versatile cytochrome P450 enzyme CYP109B1 from Bacillus subtilis		Description: The crystal structure of the versatile cytochrome P450 enzyme CYP109B1 from Bacillus subtilis
		+	[[Category: Unreleased Structures]]
		+	[[Category: Hutchinson, S]]
		+	[[Category: Zhang, A.L]]
		+	[[Category: Hall, E.A]]
		+	[[Category: Zhang, T]]
		+	[[Category: Zhouw, W.H]]
		+	[[Category: Wong, L.-L]]
		+	[[Category: Bell, S.G]]
		+	[[Category: Cryle, M.J]]

OCA: Protected "4rm4" [edit=sysop:move=sysop]

OCA — Wed, 29 Oct 2014 10:09:12 GMT

Protected "4rm4" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:09, 29 October 2014

OCA: New page: '''Unreleased structure''' The entry 4rm4 is ON HOLD Authors: Zhouw, W.H., Zhang, A.L., Zhang, T., Hall, E.A., Hutchinson, S., Cryle, M.J., Wong, L.-L., Bell, S.G. Description: The cry...

OCA — Wed, 29 Oct 2014 10:09:11 GMT

New page: '''Unreleased structure''' The entry 4rm4 is ON HOLD Authors: Zhouw, W.H., Zhang, A.L., Zhang, T., Hall, E.A., Hutchinson, S., Cryle, M.J., Wong, L.-L., Bell, S.G. Description: The cry...

New page

'''Unreleased structure'''

The entry 4rm4 is ON HOLD

Authors: Zhouw, W.H., Zhang, A.L., Zhang, T., Hall, E.A., Hutchinson, S., Cryle, M.J., Wong, L.-L., Bell, S.G.

Description: The crystal structure of the versatile cytochrome P450 enzyme CYP109B1 from Bacillus subtilis