4rp8 - Revision history

OCA at 15:15, 8 November 2023

OCA — Wed, 08 Nov 2023 15:15:45 GMT

←Older revision		Revision as of 15:15, 8 November 2023
Line 1:		Line 1:

	==Bacterial vitamin C transporter UlaA/SgaT in P21 form==		==Bacterial vitamin C transporter UlaA/SgaT in P21 form==
-	<StructureSection load='4rp8' size='340' side='right'caption='[[4rp8]]' scene=''>	+	<StructureSection load='4rp8' size='340' side='right'caption='[[4rp8]], [[Resolution\|resolution]] 2.36Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RP8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4rp8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RP8 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rp8 OCA], [https://pdbe.org/4rp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rp8 RCSB], [https://www.ebi.ac.uk/pdbsum/4rp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rp8 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.359Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASC:ASCORBIC+ACID'>ASC</scene>, <scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rp8 OCA], [https://pdbe.org/4rp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rp8 RCSB], [https://www.ebi.ac.uk/pdbsum/4rp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rp8 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ULAA_ECOLI ULAA_ECOLI] The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active-transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in ascorbate transport.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacteria use vitamin C (L-ascorbic acid) as a carbon source under anaerobic conditions. The phosphoenolpyruvate-dependent phosphotransferase system (PTS), comprising a transporter (UlaA), a IIB-like enzyme (UlaB) and a IIA-like enzyme (UlaC), is required for the anaerobic uptake of vitamin C and its phosphorylation to L-ascorbate 6-phosphate. Here, we present the crystal structures of vitamin C-bound UlaA from Escherichia coli in two conformations at 1.65-A and 2.35-A resolution. UlaA forms a homodimer and exhibits a new fold. Each UlaA protomer consists of 11 transmembrane segments arranged into a 'V-motif' domain and a 'core' domain. The V motifs form the interface between the two protomers, and the core-domain residues coordinate vitamin C. The alternating access of the substrate from the opposite side of the cell membrane may be achieved through rigid-body rotation of the core relative to the V motif.
		+
		+	Crystal structure of a phosphorylation-coupled vitamin C transporter.,Luo P, Yu X, Wang W, Fan S, Li X, Wang J Nat Struct Mol Biol. 2015 Feb 16. doi: 10.1038/nsmb.2975. PMID:25686089<ref>PMID:25686089</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4rp8" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Wang JW]]		[[Category: Wang JW]]

OCA at 10:59, 31 August 2022

OCA — Wed, 31 Aug 2022 10:59:26 GMT

←Older revision		Revision as of 10:59, 31 August 2022
Line 1:		Line 1:

	==Bacterial vitamin C transporter UlaA/SgaT in P21 form==		==Bacterial vitamin C transporter UlaA/SgaT in P21 form==
-	<StructureSection load='4rp8' size='340' side='right'caption='[[4rp8]]~~, [[Resolution\|resolution]] 2.36Å~~' scene=''>	+	<StructureSection load='4rp8' size='340' side='right'caption='[[4rp8]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[4rp8]] is a 2 chain structure.~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RP8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RP8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASC:ASCORBIC+ACID'>ASC</scene>, <scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rp8 OCA], [https://pdbe.org/4rp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rp8 RCSB], [https://www.ebi.ac.uk/pdbsum/4rp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rp8 ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4rp9\|4rp9]]</div></td></tr>~~	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rp8 OCA], [https://pdbe.org/4rp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rp8 RCSB], [https://www.ebi.ac.uk/pdbsum/4rp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rp8 ProSAT]</span></td></tr>	+
	</table>		</table>
-	== Function ==
-	[[https://www.uniprot.org/uniprot/ULAA_ECOLI ULAA_ECOLI]] The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active-transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in ascorbate transport.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Bacteria use vitamin C (L-ascorbic acid) as a carbon source under anaerobic conditions. The phosphoenolpyruvate-dependent phosphotransferase system (PTS), comprising a transporter (UlaA), a IIB-like enzyme (UlaB) and a IIA-like enzyme (UlaC), is required for the anaerobic uptake of vitamin C and its phosphorylation to L-ascorbate 6-phosphate. Here, we present the crystal structures of vitamin C-bound UlaA from Escherichia coli in two conformations at 1.65-A and 2.35-A resolution. UlaA forms a homodimer and exhibits a new fold. Each UlaA protomer consists of 11 transmembrane segments arranged into a 'V-motif' domain and a 'core' domain. The V motifs form the interface between the two protomers, and the core-domain residues coordinate vitamin C. The alternating access of the substrate from the opposite side of the cell membrane may be achieved through rigid-body rotation of the core relative to the V motif.
-
-	Crystal structure of a phosphorylation-coupled vitamin C transporter.,Luo P, Yu X, Wang W, Fan S, Li X, Wang J Nat Struct Mol Biol. 2015 Feb 16. doi: 10.1038/nsmb.2975. PMID:25686089<ref>PMID:25686089</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4rp8" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Wang~~, J W]]~~	+	[[Category: Wang JW]]
-	~~[[Category: L-ascorbate]]~~	+
-	~~[[Category: L-ascorbate-6-p]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Pt]]~~	+
-	~~[[Category: Vitamin c transporter~~]]	+

OCA at 05:08, 25 August 2022

OCA — Thu, 25 Aug 2022 05:08:51 GMT

←Older revision		Revision as of 05:08, 25 August 2022
Line 3:		Line 3:
	<StructureSection load='4rp8' size='340' side='right'caption='[[4rp8]], [[Resolution\|resolution]] 2.36Å' scene=''>		<StructureSection load='4rp8' size='340' side='right'caption='[[4rp8]], [[Resolution\|resolution]] 2.36Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4rp8]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RP8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4RP8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4rp8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RP8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASC:ASCORBIC+ACID'>ASC</scene>, <scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASC:ASCORBIC+ACID'>ASC</scene>, <scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">~~[[4rp9\|4rp9]]~~<~~/td></tr>~~	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4rp9\|4rp9]]</div></td></tr>
-	~~<tr id~~='~~gene~~'~~><td class="sblockLbl"><b~~>[[~~Gene~~\|~~Gene:~~]]</b>~~</td><td class="sblockDat">ulaA, sgaT, yjfS, b4193, JW5744 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rp8 OCA], [https://pdbe.org/4rp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rp8 RCSB], [https://www.ebi.ac.uk/pdbsum/4rp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rp8 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4rp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rp8 OCA], [~~http~~://pdbe.org/4rp8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4rp8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4rp8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4rp8 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ULAA_ECOLI ULAA_ECOLI]] The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active-transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in ascorbate transport.	+	[[https://www.uniprot.org/uniprot/ULAA_ECOLI ULAA_ECOLI]] The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active-transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in ascorbate transport.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Ecoli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Wang, J W]]		[[Category: Wang, J W]]

OCA at 10:33, 1 May 2019

OCA — Wed, 01 May 2019 10:33:18 GMT

←Older revision		Revision as of 10:33, 1 May 2019
Line 1:		Line 1:

	==Bacterial vitamin C transporter UlaA/SgaT in P21 form==		==Bacterial vitamin C transporter UlaA/SgaT in P21 form==
-	<StructureSection load='4rp8' size='340' side='right' caption='[[4rp8]], [[Resolution\|resolution]] 2.36Å' scene=''>	+	<StructureSection load='4rp8' size='340' side='right'caption='[[4rp8]], [[Resolution\|resolution]] 2.36Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4rp8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RP8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RP8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4rp8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RP8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RP8 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASC:ASCORBIC+ACID'>ASC</scene>, <scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASC:ASCORBIC+ACID'>ASC</scene>, <scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4rp9\|4rp9]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4rp9\|4rp9]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ulaA, sgaT, yjfS, b4193, JW5744 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rp8 OCA], [http://pdbe.org/4rp8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rp8 RCSB], [http://www.ebi.ac.uk/pdbsum/4rp8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rp8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rp8 OCA], [http://pdbe.org/4rp8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rp8 RCSB], [http://www.ebi.ac.uk/pdbsum/4rp8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rp8 ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Wang, J W]]		[[Category: Wang, J W]]
	[[Category: L-ascorbate]]		[[Category: L-ascorbate]]

OCA at 17:30, 18 January 2017

OCA — Wed, 18 Jan 2017 17:30:58 GMT

←Older revision		Revision as of 17:30, 18 January 2017
Line 1:		Line 1:
		+
	==Bacterial vitamin C transporter UlaA/SgaT in P21 form==		==Bacterial vitamin C transporter UlaA/SgaT in P21 form==
	<StructureSection load='4rp8' size='340' side='right' caption='[[4rp8]], [[Resolution\|resolution]] 2.36Å' scene=''>		<StructureSection load='4rp8' size='340' side='right' caption='[[4rp8]], [[Resolution\|resolution]] 2.36Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASC:ASCORBIC+ACID'>ASC</scene>, <scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASC:ASCORBIC+ACID'>ASC</scene>, <scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4rp9\|4rp9]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4rp9\|4rp9]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rp8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rp8 RCSB], [http://www.ebi.ac.uk/pdbsum/4rp8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rp8 OCA], [http://pdbe.org/4rp8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rp8 RCSB], [http://www.ebi.ac.uk/pdbsum/4rp8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rp8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4rp8" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:17, 11 March 2015

OCA — Wed, 11 Mar 2015 06:17:59 GMT

←Older revision		Revision as of 06:17, 11 March 2015
Line 1:		Line 1:
-	==~~Structure of a Membrane Protein~~==	+	==Bacterial vitamin C transporter UlaA/SgaT in P21 form==
	<StructureSection load='4rp8' size='340' side='right' caption='[[4rp8]], [[Resolution\|resolution]] 2.36Å' scene=''>		<StructureSection load='4rp8' size='340' side='right' caption='[[4rp8]], [[Resolution\|resolution]] 2.36Å' scene=''>
	== Structural highlights ==		== Structural highlights ==

OCA at 11:25, 4 March 2015

OCA — Wed, 04 Mar 2015 11:25:18 GMT

←Older revision		Revision as of 11:25, 4 March 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Structure of a Membrane Protein==
		+	<StructureSection load='4rp8' size='340' side='right' caption='[[4rp8]], [[Resolution\|resolution]] 2.36Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4rp8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RP8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RP8 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASC:ASCORBIC+ACID'>ASC</scene>, <scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4rp9\|4rp9]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rp8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rp8 RCSB], [http://www.ebi.ac.uk/pdbsum/4rp8 PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ULAA_ECOLI ULAA_ECOLI]] The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active-transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in ascorbate transport.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacteria use vitamin C (L-ascorbic acid) as a carbon source under anaerobic conditions. The phosphoenolpyruvate-dependent phosphotransferase system (PTS), comprising a transporter (UlaA), a IIB-like enzyme (UlaB) and a IIA-like enzyme (UlaC), is required for the anaerobic uptake of vitamin C and its phosphorylation to L-ascorbate 6-phosphate. Here, we present the crystal structures of vitamin C-bound UlaA from Escherichia coli in two conformations at 1.65-A and 2.35-A resolution. UlaA forms a homodimer and exhibits a new fold. Each UlaA protomer consists of 11 transmembrane segments arranged into a 'V-motif' domain and a 'core' domain. The V motifs form the interface between the two protomers, and the core-domain residues coordinate vitamin C. The alternating access of the substrate from the opposite side of the cell membrane may be achieved through rigid-body rotation of the core relative to the V motif.

-	~~The entry 4rp8 is ON HOLD until Paper Publication~~	+	Crystal structure of a phosphorylation-coupled vitamin C transporter.,Luo P, Yu X, Wang W, Fan S, Li X, Wang J Nat Struct Mol Biol. 2015 Feb 16. doi: 10.1038/nsmb.2975. PMID:25686089<ref>PMID:25686089</ref>

-	~~Authors~~: Wang, J.W.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Structure of a~~ Membrane ~~Protein~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
-	[[Category: ~~Wang, J.W~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Wang, J W]]
		+	[[Category: L-ascorbate]]
		+	[[Category: L-ascorbate-6-p]]
		+	[[Category: Membrane protein]]
		+	[[Category: Pt]]
		+	[[Category: Vitamin c transporter]]

OCA at 17:09, 24 December 2014

OCA — Wed, 24 Dec 2014 17:09:39 GMT

←Older revision		Revision as of 17:09, 24 December 2014
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	Description: Structure of a Membrane Protein		Description: Structure of a Membrane Protein
		+	[[Category: Unreleased Structures]]
		+	[[Category: Wang, J.W]]

OCA at 11:28, 19 November 2014

OCA — Wed, 19 Nov 2014 11:28:41 GMT

←Older revision		Revision as of 11:28, 19 November 2014
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4rp8 is ON HOLD	+	The entry 4rp8 is ON HOLD until Paper Publication

	Authors: Wang, J.W.		Authors: Wang, J.W.

	Description: Structure of a Membrane Protein		Description: Structure of a Membrane Protein

OCA: Protected "4rp8" [edit=sysop:move=sysop]

OCA — Wed, 05 Nov 2014 07:43:01 GMT

Protected "4rp8" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:43, 5 November 2014