4rqy - Revision history

OCA at 12:54, 1 March 2024

OCA — Fri, 01 Mar 2024 12:54:52 GMT

←Older revision		Revision as of 12:54, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4rqy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RQY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RQY FirstGlance]. <br>		<table><tr><td colspan='2'>[[4rqy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RQY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RQY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.204Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rqy OCA], [https://pdbe.org/4rqy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rqy RCSB], [https://www.ebi.ac.uk/pdbsum/4rqy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rqy ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rqy OCA], [https://pdbe.org/4rqy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rqy RCSB], [https://www.ebi.ac.uk/pdbsum/4rqy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rqy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/DEGS_ECOLI DEGS_ECOLI] When heat shock or other environmental stresses disrupt protein folding in the periplasm, DegS senses the accumulation of unassembled outer membrane porins (OMPs) and then initiates RseA (anti sigma-E factor) degradation by cleaving it in its periplasmic domain, making it an attractive substrate for subsequent cleavage by RseP. This cascade that ultimately leads to the sigma-E-driven expression of a variety of factors dealing with folding stress in the periplasm and OMP assembly.<ref>PMID:12183369</ref> <ref>PMID:19695325</ref>		[https://www.uniprot.org/uniprot/DEGS_ECOLI DEGS_ECOLI] When heat shock or other environmental stresses disrupt protein folding in the periplasm, DegS senses the accumulation of unassembled outer membrane porins (OMPs) and then initiates RseA (anti sigma-E factor) degradation by cleaving it in its periplasmic domain, making it an attractive substrate for subsequent cleavage by RseP. This cascade that ultimately leads to the sigma-E-driven expression of a variety of factors dealing with folding stress in the periplasm and OMP assembly.<ref>PMID:12183369</ref> <ref>PMID:19695325</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	In E. coli, outer-membrane stress causes a transcriptional response through a signaling cascade initiated by DegS cleavage of a transmembrane antisigma factor. Each subunit of DegS, an HtrA-family protease, contains a protease domain and a PDZ domain. The trimeric protease domain is autoinhibited by the unliganded PDZ domains. Allosteric activation requires binding of unassembled outer-membrane proteins (OMPs) to the PDZ domains and protein substrate binding. Here, we identify a set of DegS residues that cluster together at subunit-subunit interfaces in the trimer, link the active sites and substrate binding sites, and are crucial for stabilizing the active enzyme conformation in response to OMP signaling. These residues are conserved across the HtrA-protease family, including orthologs linked to human disease, supporting a common mechanism of allosteric activation. Indeed, mutation of residues at homologous positions in the DegP quality-control protease also eliminates allosteric activation.
-
-	A Conserved Activation Cluster Is Required for Allosteric Communication in HtrA-Family Proteases.,de Regt AK, Kim S, Sohn J, Grant RA, Baker TA, Sauer RT Structure. 2015 Mar 3;23(3):517-26. doi: 10.1016/j.str.2015.01.012. Epub 2015 Feb, 19. PMID:25703375<ref>PMID:25703375</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4rqy" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:24, 8 March 2023

OCA — Wed, 08 Mar 2023 08:24:57 GMT

←Older revision		Revision as of 08:24, 8 March 2023
Line 3:		Line 3:
	<StructureSection load='4rqy' size='340' side='right'caption='[[4rqy]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4rqy' size='340' side='right'caption='[[4rqy]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4rqy]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RQY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4RQY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4rqy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RQY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RQY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1te0\|1te0]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rqy OCA], [https://pdbe.org/4rqy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rqy RCSB], [https://www.ebi.ac.uk/pdbsum/4rqy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rqy ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">degS, P12B_c3347 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4rqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rqy OCA], [~~http~~://pdbe.org/4rqy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4rqy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4rqy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4rqy ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/DEGS_ECOLI DEGS_ECOLI] When heat shock or other environmental stresses disrupt protein folding in the periplasm, DegS senses the accumulation of unassembled outer membrane porins (OMPs) and then initiates RseA (anti sigma-E factor) degradation by cleaving it in its periplasmic domain, making it an attractive substrate for subsequent cleavage by RseP. This cascade that ultimately leads to the sigma-E-driven expression of a variety of factors dealing with folding stress in the periplasm and OMP assembly.<ref>PMID:12183369</ref> <ref>PMID:19695325</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
-	~~Regulated proteolysis is a key event in transmembrane signalling between intracellular compartments~~. ~~In Escherichia~~ coli ~~the membrane-bound protease DegS has been identified as the periplasmic stress sensor for unfolded~~ outer membrane ~~proteins (OMPs). DegS inititates~~ a ~~proteolytic~~ cascade ~~resulting in the release~~ of ~~sigmaE the transcription~~ factor ~~of periplasmic genes~~. ~~The crystal structure~~ of DegS protease ~~reported at 2.2 A resolution reveals~~ a trimeric ~~complex with the monomeric~~ protease domain ~~in an inhibited state followed~~ by the ~~inhibitory~~ PDZ ~~domain~~. ~~Noteably, domain architecture and communication~~ of ~~DegS are remarkably to homologous~~ proteins ~~known~~ to ~~date. Here~~ the ~~domain interface is mechanically locked by three intradomain salt bridges~~. ~~Co-crystallisation trials in the presence of~~ a 10-~~residue activating peptide did not result in significant structural intradomain shifts nor distortions~~ in the ~~crystal packing~~. These ~~observations imply~~ a ~~mode~~ of activation ~~indicative~~ of ~~peptide-induced structural shifts imposed to~~ the ~~protease domain rather than disturbing the PDZ~~-protease ~~interface~~.	+	In E. coli, outer-membrane stress causes a transcriptional response through a signaling cascade initiated by DegS cleavage of a transmembrane antisigma factor. Each subunit of DegS, an HtrA-family protease, contains a protease domain and a PDZ domain. The trimeric protease domain is autoinhibited by the unliganded PDZ domains. Allosteric activation requires binding of unassembled outer-membrane proteins (OMPs) to the PDZ domains and protein substrate binding. Here, we identify a set of DegS residues that cluster together at subunit-subunit interfaces in the trimer, link the active sites and substrate binding sites, and are crucial for stabilizing the active enzyme conformation in response to OMP signaling. These residues are conserved across the HtrA-protease family, including orthologs linked to human disease, supporting a common mechanism of allosteric activation. Indeed, mutation of residues at homologous positions in the DegP quality-control protease also eliminates allosteric activation.

-	~~Structural analysis of DegS, a stress sensor of the bacterial periplasm~~.,~~Zeth K FEBS Lett~~. ~~2004 Jul 2~~;~~569~~(1-3):~~351~~-8. PMID:~~15225661~~<ref>PMID:~~15225661~~</ref>	+	A Conserved Activation Cluster Is Required for Allosteric Communication in HtrA-Family Proteases.,de Regt AK, Kim S, Sohn J, Grant RA, Baker TA, Sauer RT Structure. 2015 Mar 3;23(3):517-26. doi: 10.1016/j.str.2015.01.012. Epub 2015 Feb, 19. PMID:25703375<ref>PMID:25703375</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Grant~~, R A~~]]	+	[[Category: Grant RA]]
-	[[Category: Sauer~~, R T]]~~	+	[[Category: Sauer RT]]
-	~~[[Category: Alpha-beta protein]]~~	+
-	~~[[Category: Htra]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Pdz]]~~	+
-	~~[[Category: Serine protease]]~~	+
-	~~[[Category: Stress response]]~~	+
-	~~[[Category: Two domain~~]]	+

OCA at 10:27, 1 May 2019

OCA — Wed, 01 May 2019 10:27:49 GMT

←Older revision		Revision as of 10:27, 1 May 2019
Line 1:		Line 1:

	==RE-REFINED STRUCTURE OF 1TE0 - STRUCTURAL ANALYSIS of DEGS, A STRESS SENSOR OF THE BACTERIAL PERIPLASM==		==RE-REFINED STRUCTURE OF 1TE0 - STRUCTURAL ANALYSIS of DEGS, A STRESS SENSOR OF THE BACTERIAL PERIPLASM==
-	<StructureSection load='4rqy' size='340' side='right' caption='[[4rqy]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='4rqy' size='340' side='right'caption='[[4rqy]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4rqy]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RQY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RQY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4rqy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RQY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RQY FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1te0\|1te0]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1te0\|1te0]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">degS, P12B_c3347 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rqy OCA], [http://pdbe.org/4rqy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rqy RCSB], [http://www.ebi.ac.uk/pdbsum/4rqy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rqy ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rqy OCA], [http://pdbe.org/4rqy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rqy RCSB], [http://www.ebi.ac.uk/pdbsum/4rqy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rqy ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Grant, R A]]		[[Category: Grant, R A]]
	[[Category: Sauer, R T]]		[[Category: Sauer, R T]]

OCA at 17:27, 18 January 2017

OCA — Wed, 18 Jan 2017 17:27:49 GMT

←Older revision		Revision as of 17:27, 18 January 2017
Line 1:		Line 1:
		+
	==RE-REFINED STRUCTURE OF 1TE0 - STRUCTURAL ANALYSIS of DEGS, A STRESS SENSOR OF THE BACTERIAL PERIPLASM==		==RE-REFINED STRUCTURE OF 1TE0 - STRUCTURAL ANALYSIS of DEGS, A STRESS SENSOR OF THE BACTERIAL PERIPLASM==
	<StructureSection load='4rqy' size='340' side='right' caption='[[4rqy]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4rqy' size='340' side='right' caption='[[4rqy]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1te0\|1te0]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1te0\|1te0]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rqy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rqy RCSB], [http://www.ebi.ac.uk/pdbsum/4rqy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rqy OCA], [http://pdbe.org/4rqy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rqy RCSB], [http://www.ebi.ac.uk/pdbsum/4rqy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rqy ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4rqy" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:45, 11 March 2015

OCA — Wed, 11 Mar 2015 11:45:23 GMT

←Older revision		Revision as of 11:45, 11 March 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==RE-REFINED STRUCTURE OF 1TE0 - STRUCTURAL ANALYSIS of DEGS, A STRESS SENSOR OF THE BACTERIAL PERIPLASM==
		+	<StructureSection load='4rqy' size='340' side='right' caption='[[4rqy]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4rqy]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RQY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RQY FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1te0\|1te0]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rqy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rqy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rqy RCSB], [http://www.ebi.ac.uk/pdbsum/4rqy PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Regulated proteolysis is a key event in transmembrane signalling between intracellular compartments. In Escherichia coli the membrane-bound protease DegS has been identified as the periplasmic stress sensor for unfolded outer membrane proteins (OMPs). DegS inititates a proteolytic cascade resulting in the release of sigmaE the transcription factor of periplasmic genes. The crystal structure of DegS protease reported at 2.2 A resolution reveals a trimeric complex with the monomeric protease domain in an inhibited state followed by the inhibitory PDZ domain. Noteably, domain architecture and communication of DegS are remarkably to homologous proteins known to date. Here the domain interface is mechanically locked by three intradomain salt bridges. Co-crystallisation trials in the presence of a 10-residue activating peptide did not result in significant structural intradomain shifts nor distortions in the crystal packing. These observations imply a mode of activation indicative of peptide-induced structural shifts imposed to the protease domain rather than disturbing the PDZ-protease interface.

-	~~The entry 4rqy is ON HOLD~~	+	Structural analysis of DegS, a stress sensor of the bacterial periplasm.,Zeth K FEBS Lett. 2004 Jul 2;569(1-3):351-8. PMID:15225661<ref>PMID:15225661</ref>

-	~~Authors: Sauer~~, R.T., Grant, R.A.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: RE-~~REFINED STRUCTURE OF 1TE0 -STRUCTURAL ANALYSIS of DEGS, A STRESS SENSOR OF THE BACTERIAL PERIPLASM~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
-	[[Category: ~~Sauer, R.T~~]]	+	__TOC__
-	[[Category: ~~Grant, R.A~~]]	+	</StructureSection>
		+	[[Category: Grant, R A]]
		+	[[Category: Sauer, R T]]
		+	[[Category: Alpha-beta protein]]
		+	[[Category: Htra]]
		+	[[Category: Hydrolase]]
		+	[[Category: Pdz]]
		+	[[Category: Serine protease]]
		+	[[Category: Stress response]]
		+	[[Category: Two domain]]

OCA at 17:14, 24 December 2014

OCA — Wed, 24 Dec 2014 17:14:22 GMT

←Older revision		Revision as of 17:14, 24 December 2014
Line 6:		Line 6:

	Description: RE-REFINED STRUCTURE OF 1TE0 -STRUCTURAL ANALYSIS of DEGS, A STRESS SENSOR OF THE BACTERIAL PERIPLASM		Description: RE-REFINED STRUCTURE OF 1TE0 -STRUCTURAL ANALYSIS of DEGS, A STRESS SENSOR OF THE BACTERIAL PERIPLASM
		+	[[Category: Unreleased Structures]]
		+	[[Category: Sauer, R.T]]
		+	[[Category: Grant, R.A]]

OCA at 11:05, 17 December 2014

OCA — Wed, 17 Dec 2014 11:05:02 GMT

←Older revision		Revision as of 11:05, 17 December 2014
Line 5:		Line 5:
	Authors: Sauer, R.T., Grant, R.A.		Authors: Sauer, R.T., Grant, R.A.

-	Description: re-~~refined structure of 1te0~~ -STRUCTURAL ANALYSIS OF DEGS, A STRESS SENSOR OF THE BACTERIAL PERIPLASM	+	Description: RE-REFINED STRUCTURE OF 1TE0 -STRUCTURAL ANALYSIS of DEGS, A STRESS SENSOR OF THE BACTERIAL PERIPLASM

OCA: Protected "4rqy" [edit=sysop:move=sysop]

OCA — Wed, 19 Nov 2014 11:30:54 GMT

Protected "4rqy" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:30, 19 November 2014

OCA: New page: '''Unreleased structure''' The entry 4rqy is ON HOLD Authors: Sauer, R.T., Grant, R.A. Description: re-refined structure of 1te0 -STRUCTURAL ANALYSIS OF DEGS, A STRESS SENSOR OF THE BA...

OCA — Wed, 19 Nov 2014 11:30:53 GMT

New page: '''Unreleased structure''' The entry 4rqy is ON HOLD Authors: Sauer, R.T., Grant, R.A. Description: re-refined structure of 1te0 -STRUCTURAL ANALYSIS OF DEGS, A STRESS SENSOR OF THE BA...

New page

'''Unreleased structure'''

The entry 4rqy is ON HOLD

Authors: Sauer, R.T., Grant, R.A.

Description: re-refined structure of 1te0 -STRUCTURAL ANALYSIS OF DEGS, A STRESS SENSOR OF THE BACTERIAL PERIPLASM