4ryx - Revision history

OCA at 17:59, 20 September 2023

OCA — Wed, 20 Sep 2023 17:59:17 GMT

←Older revision		Revision as of 17:59, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ryx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RYX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RYX FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ryx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RYX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RYX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3V:(1R)-3-AMINO-1-[3-(CYCLOHEXYLMETHOXY)PHENYL]PROPAN-1-OL'>A3V</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3V:(1R)-3-AMINO-1-[3-(CYCLOHEXYLMETHOXY)PHENYL]PROPAN-1-OL'>A3V</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ryx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ryx OCA], [https://pdbe.org/4ryx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ryx RCSB], [https://www.ebi.ac.uk/pdbsum/4ryx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ryx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ryx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ryx OCA], [https://pdbe.org/4ryx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ryx RCSB], [https://www.ebi.ac.uk/pdbsum/4ryx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ryx ProSAT]</span></td></tr>
	</table>		</table>

OCA at 20:58, 12 April 2023

OCA — Wed, 12 Apr 2023 20:58:13 GMT

←Older revision		Revision as of 20:58, 12 April 2023
Line 5:		Line 5:
	<table><tr><td colspan='2'>[[4ryx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RYX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RYX FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ryx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RYX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RYX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3V:(1R)-3-AMINO-1-[3-(CYCLOHEXYLMETHOXY)PHENYL]PROPAN-1-OL'>A3V</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3V:(1R)-3-AMINO-1-[3-(CYCLOHEXYLMETHOXY)PHENYL]PROPAN-1-OL'>A3V</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3fsn\|3fsn]], [[4rsc\|4rsc]], [[4ryy\|4ryy]], [[4ryz\|4ryz]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Retinoid_isomerohydrolase Retinoid isomerohydrolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.64 3.1.1.64] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ryx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ryx OCA], [https://pdbe.org/4ryx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ryx RCSB], [https://www.ebi.ac.uk/pdbsum/4ryx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ryx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ryx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ryx OCA], [https://pdbe.org/4ryx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ryx RCSB], [https://www.ebi.ac.uk/pdbsum/4ryx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ryx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/RPE65_BOVIN RPE65_BOVIN]] Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.<ref>PMID:16096063</ref> <ref>PMID:19805034</ref> <ref>PMID:20100834</ref>	+	[https://www.uniprot.org/uniprot/RPE65_BOVIN RPE65_BOVIN] Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.<ref>PMID:16096063</ref> <ref>PMID:19805034</ref> <ref>PMID:20100834</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 29:		Line 27:
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Retinoid isomerohydrolase]]~~	+	[[Category: Kiser PD]]
-	[[Category: Kiser~~, P D~~]]	+	[[Category: Palczewski K]]
-	[[Category: Palczewski, K]]	+
-	~~[[Category: 7-bladed beta propeller]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Isomerase]]~~	+
-	~~[[Category: Monotopic membrane protein]]~~	+
-	~~[[Category: Non-heme iron enzyme]]~~	+
-	~~[[Category: Retinoid isomerase~~]]	+

OCA at 10:00, 7 July 2021

OCA — Wed, 07 Jul 2021 10:00:38 GMT

←Older revision		Revision as of 10:00, 7 July 2021
Line 3:		Line 3:
	<StructureSection load='4ryx' size='340' side='right'caption='[[4ryx]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4ryx' size='340' side='right'caption='[[4ryx]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ryx]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RYX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4RYX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ryx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RYX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RYX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3V:(1R)-3-AMINO-1-[3-(CYCLOHEXYLMETHOXY)PHENYL]PROPAN-1-OL'>A3V</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3V:(1R)-3-AMINO-1-[3-(CYCLOHEXYLMETHOXY)PHENYL]PROPAN-1-OL'>A3V</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fsn\|3fsn]], [[4rsc\|4rsc]], [[4ryy\|4ryy]], [[4ryz\|4ryz]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3fsn\|3fsn]], [[4rsc\|4rsc]], [[4ryy\|4ryy]], [[4ryz\|4ryz]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Retinoid_isomerohydrolase Retinoid isomerohydrolase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.64 3.1.1.64] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Retinoid_isomerohydrolase Retinoid isomerohydrolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.64 3.1.1.64] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ryx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ryx OCA], [~~http~~://pdbe.org/4ryx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ryx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ryx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ryx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ryx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ryx OCA], [https://pdbe.org/4ryx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ryx RCSB], [https://www.ebi.ac.uk/pdbsum/4ryx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ryx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/RPE65_BOVIN RPE65_BOVIN]] Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.<ref>PMID:16096063</ref> <ref>PMID:19805034</ref> <ref>PMID:20100834</ref>	+	[[https://www.uniprot.org/uniprot/RPE65_BOVIN RPE65_BOVIN]] Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.<ref>PMID:16096063</ref> <ref>PMID:19805034</ref> <ref>PMID:20100834</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Emixustat is a visual cycle modulator that has entered clinical trials as a treatment for age-related macular degeneration (AMD). This molecule has been proposed to inhibit the visual cycle isomerase RPE65, thereby slowing regeneration of 11-cis-retinal and reducing production of retinaldehyde condensation byproducts that may be involved in AMD pathology. Previously, we reported that all-trans-retinal (atRAL) is directly cytotoxic and that certain primary amine compounds that transiently sequester atRAL via Schiff base formation ameliorate retinal degeneration. Here, we have shown that emixustat stereoselectively inhibits RPE65 by direct active site binding. However, we detected the presence of emixustat-atRAL Schiff base conjugates, indicating that emixustat also acts as a retinal scavenger, which may contribute to its therapeutic effects. Using agents that lack either RPE65 inhibitory activity or the capacity to sequester atRAL, we assessed the relative importance of these 2 modes of action in protection against retinal phototoxicity in mice. The atRAL sequestrant QEA-B-001-NH2 conferred protection against phototoxicity without inhibiting RPE65, whereas an emixustat derivative incapable of atRAL sequestration was minimally protective, despite direct inhibition of RPE65. These data indicate that atRAL sequestration is an essential mechanism underlying the protective effects of emixustat and related compounds against retinal phototoxicity. Moreover, atRAL sequestration should be considered in the design of next-generation visual cycle modulators.
		+
		+	Molecular pharmacodynamics of emixustat in protection against retinal degeneration.,Zhang J, Kiser PD, Badiee M, Palczewska G, Dong Z, Golczak M, Tochtrop GP, Palczewski K J Clin Invest. 2015 Jul 1;125(7):2781-94. doi: 10.1172/JCI80950. Epub 2015 Jun, 15. PMID:26075817<ref>PMID:26075817</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4ryx" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 10:32, 1 May 2019

OCA — Wed, 01 May 2019 10:32:35 GMT

←Older revision		Revision as of 10:32, 1 May 2019
Line 1:		Line 1:

	==Crystal structure of RPE65 in complex with emixustat and palmitate, P6522 crystal form==		==Crystal structure of RPE65 in complex with emixustat and palmitate, P6522 crystal form==
-	<StructureSection load='4ryx' size='340' side='right' caption='[[4ryx]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='4ryx' size='340' side='right'caption='[[4ryx]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ryx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RYX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RYX FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ryx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RYX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RYX FirstGlance]. <br>
Line 19:		Line 19:
	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
		+	[[Category: Large Structures]]
	[[Category: Retinoid isomerohydrolase]]		[[Category: Retinoid isomerohydrolase]]
	[[Category: Kiser, P D]]		[[Category: Kiser, P D]]

OCA at 01:57, 10 March 2017

OCA — Fri, 10 Mar 2017 01:57:44 GMT

←Older revision		Revision as of 01:57, 10 March 2017
Line 1:		Line 1:
		+
	==Crystal structure of RPE65 in complex with emixustat and palmitate, P6522 crystal form==		==Crystal structure of RPE65 in complex with emixustat and palmitate, P6522 crystal form==
	<StructureSection load='4ryx' size='340' side='right' caption='[[4ryx]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4ryx' size='340' side='right' caption='[[4ryx]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fsn\|3fsn]], [[4rsc\|4rsc]], [[4ryy\|4ryy]], [[4ryz\|4ryz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fsn\|3fsn]], [[4rsc\|4rsc]], [[4ryy\|4ryy]], [[4ryz\|4ryz]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Retinoid_isomerohydrolase Retinoid isomerohydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.64 3.1.1.64] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Retinoid_isomerohydrolase Retinoid isomerohydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.64 3.1.1.64] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ryx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ryx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ryx RCSB], [http://www.ebi.ac.uk/pdbsum/4ryx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ryx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ryx OCA], [http://pdbe.org/4ryx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ryx RCSB], [http://www.ebi.ac.uk/pdbsum/4ryx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ryx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/RPE65_BOVIN RPE65_BOVIN]] Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.<ref>PMID:16096063</ref> <ref>PMID:19805034</ref> <ref>PMID:20100834</ref>		[[http://www.uniprot.org/uniprot/RPE65_BOVIN RPE65_BOVIN]] Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.<ref>PMID:16096063</ref> <ref>PMID:19805034</ref> <ref>PMID:20100834</ref>
		+
		+	==See Also==
		+	*[[Retinoid isomerohydrolase\|Retinoid isomerohydrolase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 15:57, 27 May 2015

OCA — Wed, 27 May 2015 15:57:28 GMT

←Older revision		Revision as of 15:57, 27 May 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal structure of RPE65 in complex with emixustat and palmitate, P6522 crystal form==
-		+	<StructureSection load='4ryx' size='340' side='right' caption='[[4ryx]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~The entry~~ 4ryx is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4ryx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RYX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RYX FirstGlance]. <br>
-	~~Authors~~: ~~Kiser~~, P.D., ~~Palczewski~~, K.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3V:(1R)-3-AMINO-1-[3-(CYCLOHEXYLMETHOXY)PHENYL]PROPAN-1-OL'>A3V</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3fsn\|3fsn]], [[4rsc\|4rsc]], [[4ryy\|4ryy]], [[4ryz\|4ryz]]</td></tr>
-	~~Description~~: ~~Crystal structure of RPE65~~ in ~~complex with emixustat~~ and ~~palmitate~~, ~~P6522 crystal~~ form	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Retinoid_isomerohydrolase Retinoid isomerohydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.64 3.1.1.64] </span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ryx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ryx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ryx RCSB], [http://www.ebi.ac.uk/pdbsum/4ryx PDBsum]</span></td></tr>
-	[[Category: Kiser, P.D]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RPE65_BOVIN RPE65_BOVIN]] Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.<ref>PMID:16096063</ref> <ref>PMID:19805034</ref> <ref>PMID:20100834</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bos taurus]]
		+	[[Category: Retinoid isomerohydrolase]]
		+	[[Category: Kiser, P D]]
	[[Category: Palczewski, K]]		[[Category: Palczewski, K]]
		+	[[Category: 7-bladed beta propeller]]
		+	[[Category: Hydrolase]]
		+	[[Category: Isomerase]]
		+	[[Category: Monotopic membrane protein]]
		+	[[Category: Non-heme iron enzyme]]
		+	[[Category: Retinoid isomerase]]

OCA at 14:06, 21 January 2015

OCA — Wed, 21 Jan 2015 14:06:15 GMT

←Older revision		Revision as of 14:06, 21 January 2015
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4ryx is ON HOLD	+	The entry 4ryx is ON HOLD until Paper Publication

	Authors: Kiser, P.D., Palczewski, K.		Authors: Kiser, P.D., Palczewski, K.

OCA at 17:38, 24 December 2014

OCA — Wed, 24 Dec 2014 17:38:48 GMT

←Older revision		Revision as of 17:38, 24 December 2014
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	Description: Crystal structure of RPE65 in complex with emixustat and palmitate, P6522 crystal form		Description: Crystal structure of RPE65 in complex with emixustat and palmitate, P6522 crystal form
		+	[[Category: Unreleased Structures]]
		+	[[Category: Kiser, P.D]]
		+	[[Category: Palczewski, K]]

OCA: Protected "4ryx" [edit=sysop:move=sysop]

OCA — Wed, 24 Dec 2014 09:33:22 GMT

Protected "4ryx" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:33, 24 December 2014

OCA: New page: '''Unreleased structure''' The entry 4ryx is ON HOLD Authors: Kiser, P.D., Palczewski, K. Description: Crystal structure of RPE65 in complex with emixustat and palmitate, P6522 crystal...

OCA — Wed, 24 Dec 2014 09:33:21 GMT

New page: '''Unreleased structure''' The entry 4ryx is ON HOLD Authors: Kiser, P.D., Palczewski, K. Description: Crystal structure of RPE65 in complex with emixustat and palmitate, P6522 crystal...

New page

'''Unreleased structure'''

The entry 4ryx is ON HOLD

Authors: Kiser, P.D., Palczewski, K.

Description: Crystal structure of RPE65 in complex with emixustat and palmitate, P6522 crystal form