4rzb - Revision history

OCA at 12:57, 1 March 2024

OCA — Fri, 01 Mar 2024 12:57:14 GMT

←Older revision		Revision as of 12:57, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4rzb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RZB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RZB FirstGlance]. <br>		<table><tr><td colspan='2'>[[4rzb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RZB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RZB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=NFQ:N-[(E)-IMINOMETHYL]-L-ASPARTIC+ACID'>NFQ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.863Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=NFQ:N-[(E)-IMINOMETHYL]-L-ASPARTIC+ACID'>NFQ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rzb OCA], [https://pdbe.org/4rzb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rzb RCSB], [https://www.ebi.ac.uk/pdbsum/4rzb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rzb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rzb OCA], [https://pdbe.org/4rzb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rzb RCSB], [https://www.ebi.ac.uk/pdbsum/4rzb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rzb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/HUTF_PSEAE HUTF_PSEAE] Catalyzes the hydrolysis of N-formimino-L-glutamate to N-formyl-L-glutamate and ammonia.<ref>PMID:16475788</ref> <ref>PMID:17128965</ref>		[https://www.uniprot.org/uniprot/HUTF_PSEAE HUTF_PSEAE] Catalyzes the hydrolysis of N-formimino-L-glutamate to N-formyl-L-glutamate and ammonia.<ref>PMID:16475788</ref> <ref>PMID:17128965</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	N-Formimino-l-glutamate iminohydrolase (HutF), from Pseudomonas aeruginosa with a locus tag of Pa5106 ( gi\|15600299 ), is a member of the amidohydrolase superfamily. This enzyme catalyzes the deamination of N-formimino-l-glutamate to N-formyl-l-glutamate and ammonia in the histidine degradation pathway. The crystal structure of Pa5106 was determined in the presence of the inhibitors N-formimino-l-aspartate and N-guanidino-l-glutaric acid at resolutions of 1.9 and 1.4 A, respectively. The structure of an individual subunit is composed of two domains with the larger domain folding as a distorted (beta/alpha)8-barrel. The (beta/alpha)8-barrel domain is composed of eight beta-strands flanked by 11 alpha-helices, whereas the smaller domain is made up of eight beta-strands. The active site of Pa5106 contains a single zinc atom that is coordinated by His-56, His-58, His-232, and Asp-320. The nucleophilic solvent water molecule coordinates with the zinc atom at a distance of 2.0 A and is hydrogen bonded to Asp-320 and His-269. The alpha-carboxylate groups of both inhibitors are hydrogen bonded to the imidazole moiety of His-206, the hydroxyl group of Tyr-121, and the side chain amide group of Gln-61. The side chain carboxylate groups of the two inhibitors are ion-paired with the guanidino groups of Arg-209 and Arg-82. Computational docking of high-energy tetrahedral intermediate forms of the substrate, N-formimino-l-glutamate, to the three-dimensional structure of Pa5106 suggests that this compound likely undergoes a re-faced nucleophilic attack at the formimino group by the metal-bound hydroxide. A catalytic mechanism of the reaction catalyzed by Pa5106 is proposed.
-
-	Structure of N-Formimino-l-glutamate Iminohydrolase from Pseudomonas aeruginosa.,Fedorov AA, Marti-Arbona R, Nemmara VV, Hitchcock D, Fedorov EV, Almo SC, Raushel FM Biochemistry. 2015 Jan 16. PMID:25559274<ref>PMID:25559274</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4rzb" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:35, 15 March 2023

OCA — Wed, 15 Mar 2023 10:35:10 GMT

←Older revision		Revision as of 10:35, 15 March 2023
Line 3:		Line 3:
	<StructureSection load='4rzb' size='340' side='right'caption='[[4rzb]], [[Resolution\|resolution]] 1.86Å' scene=''>		<StructureSection load='4rzb' size='340' side='right'caption='[[4rzb]], [[Resolution\|resolution]] 1.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4rzb]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pseae Pseae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RZB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4RZB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4rzb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RZB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RZB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=NFQ:N-[(E)-IMINOMETHYL]-L-ASPARTIC+ACID'>NFQ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=NFQ:N-[(E)-IMINOMETHYL]-L-ASPARTIC+ACID'>NFQ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mdw\|3mdw]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rzb OCA], [https://pdbe.org/4rzb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rzb RCSB], [https://www.ebi.ac.uk/pdbsum/4rzb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rzb ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA5106 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4rzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rzb OCA], [~~http~~://pdbe.org/4rzb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4rzb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4rzb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4rzb ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/HUTF_PSEAE HUTF_PSEAE] Catalyzes the hydrolysis of N-formimino-L-glutamate to N-formyl-L-glutamate and ammonia.<ref>PMID:16475788</ref> <ref>PMID:17128965</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pseae~~]]	+	[[Category: Pseudomonas aeruginosa PAO1]]
-	[[Category: Almo~~, S C~~]]	+	[[Category: Almo SC]]
-	[[Category: Fedorov~~, A A~~]]	+	[[Category: Fedorov AA]]
-	[[Category: Fedorov~~, E V~~]]	+	[[Category: Fedorov EV]]
-	[[Category: Marti-Arbona, R]]	+	[[Category: Marti-Arbona R]]
-	[[Category: Raushel~~, F M]]~~	+	[[Category: Raushel FM]]
-	~~[[Category: Amidohydrolase fold]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: N-formimino-l-aspartate]]~~	+
-	~~[[Category: N-formimino-l-glutamate iminohydrolase~~]]	+

OCA at 10:32, 1 May 2019

OCA — Wed, 01 May 2019 10:32:21 GMT

←Older revision		Revision as of 10:32, 1 May 2019
Line 1:		Line 1:

	==The structure of N-formimino-L-Glutamate Iminohydrolase from Pseudomonas aeruginosa complexed with N-formimino-L-Aspartate, SOAKED WITH MERCURY==		==The structure of N-formimino-L-Glutamate Iminohydrolase from Pseudomonas aeruginosa complexed with N-formimino-L-Aspartate, SOAKED WITH MERCURY==
-	<StructureSection load='4rzb' size='340' side='right' caption='[[4rzb]], [[Resolution\|resolution]] 1.86Å' scene=''>	+	<StructureSection load='4rzb' size='340' side='right'caption='[[4rzb]], [[Resolution\|resolution]] 1.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4rzb]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RZB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RZB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4rzb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RZB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RZB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=NFQ:N-[(E)-IMINOMETHYL]-L-ASPARTIC+ACID'>NFQ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=NFQ:N-[(E)-IMINOMETHYL]-L-ASPARTIC+ACID'>NFQ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mdw\|3mdw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mdw\|3mdw]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA5106 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rzb OCA], [http://pdbe.org/4rzb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rzb RCSB], [http://www.ebi.ac.uk/pdbsum/4rzb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rzb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rzb OCA], [http://pdbe.org/4rzb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rzb RCSB], [http://www.ebi.ac.uk/pdbsum/4rzb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rzb ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Pseae]]
	[[Category: Almo, S C]]		[[Category: Almo, S C]]
	[[Category: Fedorov, A A]]		[[Category: Fedorov, A A]]

OCA at 01:20, 19 January 2017

OCA — Thu, 19 Jan 2017 01:20:50 GMT

←Older revision		Revision as of 01:20, 19 January 2017
Line 1:		Line 1:
		+
	==The structure of N-formimino-L-Glutamate Iminohydrolase from Pseudomonas aeruginosa complexed with N-formimino-L-Aspartate, SOAKED WITH MERCURY==		==The structure of N-formimino-L-Glutamate Iminohydrolase from Pseudomonas aeruginosa complexed with N-formimino-L-Aspartate, SOAKED WITH MERCURY==
	<StructureSection load='4rzb' size='340' side='right' caption='[[4rzb]], [[Resolution\|resolution]] 1.86Å' scene=''>		<StructureSection load='4rzb' size='340' side='right' caption='[[4rzb]], [[Resolution\|resolution]] 1.86Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=NFQ:N-[(E)-IMINOMETHYL]-L-ASPARTIC+ACID'>NFQ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=NFQ:N-[(E)-IMINOMETHYL]-L-ASPARTIC+ACID'>NFQ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mdw\|3mdw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mdw\|3mdw]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rzb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rzb RCSB], [http://www.ebi.ac.uk/pdbsum/4rzb PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rzb OCA], [http://pdbe.org/4rzb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rzb RCSB], [http://www.ebi.ac.uk/pdbsum/4rzb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rzb ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4rzb" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:28, 12 February 2015

OCA — Thu, 12 Feb 2015 06:28:11 GMT

←Older revision		Revision as of 06:28, 12 February 2015
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rzb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rzb RCSB], [http://www.ebi.ac.uk/pdbsum/4rzb PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rzb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rzb RCSB], [http://www.ebi.ac.uk/pdbsum/4rzb PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	N-Formimino-l-glutamate iminohydrolase (HutF), from Pseudomonas aeruginosa with a locus tag of Pa5106 ( gi\|15600299 ), is a member of the amidohydrolase superfamily. This enzyme catalyzes the deamination of N-formimino-l-glutamate to N-formyl-l-glutamate and ammonia in the histidine degradation pathway. The crystal structure of Pa5106 was determined in the presence of the inhibitors N-formimino-l-aspartate and N-guanidino-l-glutaric acid at resolutions of 1.9 and 1.4 A, respectively. The structure of an individual subunit is composed of two domains with the larger domain folding as a distorted (beta/alpha)8-barrel. The (beta/alpha)8-barrel domain is composed of eight beta-strands flanked by 11 alpha-helices, whereas the smaller domain is made up of eight beta-strands. The active site of Pa5106 contains a single zinc atom that is coordinated by His-56, His-58, His-232, and Asp-320. The nucleophilic solvent water molecule coordinates with the zinc atom at a distance of 2.0 A and is hydrogen bonded to Asp-320 and His-269. The alpha-carboxylate groups of both inhibitors are hydrogen bonded to the imidazole moiety of His-206, the hydroxyl group of Tyr-121, and the side chain amide group of Gln-61. The side chain carboxylate groups of the two inhibitors are ion-paired with the guanidino groups of Arg-209 and Arg-82. Computational docking of high-energy tetrahedral intermediate forms of the substrate, N-formimino-l-glutamate, to the three-dimensional structure of Pa5106 suggests that this compound likely undergoes a re-faced nucleophilic attack at the formimino group by the metal-bound hydroxide. A catalytic mechanism of the reaction catalyzed by Pa5106 is proposed.
		+
		+	Structure of N-Formimino-l-glutamate Iminohydrolase from Pseudomonas aeruginosa.,Fedorov AA, Marti-Arbona R, Nemmara VV, Hitchcock D, Fedorov EV, Almo SC, Raushel FM Biochemistry. 2015 Jan 16. PMID:25559274<ref>PMID:25559274</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 13:44, 14 January 2015

OCA — Wed, 14 Jan 2015 13:44:47 GMT

←Older revision		Revision as of 13:44, 14 January 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==The structure of N-formimino-L-Glutamate Iminohydrolase from Pseudomonas aeruginosa complexed with N-formimino-L-Aspartate, SOAKED WITH MERCURY==
-		+	<StructureSection load='4rzb' size='340' side='right' caption='[[4rzb]], [[Resolution\|resolution]] 1.86Å' scene=''>
-	~~The entry~~ 4rzb is ~~ON HOLD~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4rzb]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RZB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RZB FirstGlance]. <br>
-	~~Authors~~: ~~Fedorov, A~~.A.~~, Fedorov, E~~.~~V., Marti~~-~~Arbona, R~~.~~, Raushel, F~~.M.~~, Almo, S~~.C.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=NFQ:N-[(E)-IMINOMETHYL]-L-ASPARTIC+ACID'>NFQ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mdw\|3mdw]]</td></tr>
-	~~Description~~: ~~The structure of~~ N-~~formimino~~-~~L-Glutamate Iminohydrolase from Pseudomonas aeruginosa complexed with N-formimino~~-L-~~Aspartate~~, ~~SOAKED WITH MERCURY~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rzb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rzb RCSB], [http://www.ebi.ac.uk/pdbsum/4rzb PDBsum]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: Almo, S.C]]	+	__TOC__
-	[[Category: Fedorov, A.A]]	+	</StructureSection>
		+	[[Category: Almo, S C]]
		+	[[Category: Fedorov, A A]]
		+	[[Category: Fedorov, E V]]
	[[Category: Marti-Arbona, R]]		[[Category: Marti-Arbona, R]]
-	[[Category: ~~Fedorov~~, ~~E.V~~]]	+	[[Category: Raushel, F M]]
-	[[Category: ~~Raushel, F.M~~]]	+	[[Category: Amidohydrolase fold]]
		+	[[Category: Hydrolase]]
		+	[[Category: N-formimino-l-aspartate]]
		+	[[Category: N-formimino-l-glutamate iminohydrolase]]

OCA: Protected "4rzb" [edit=sysop:move=sysop]

OCA — Wed, 31 Dec 2014 12:16:21 GMT

Protected "4rzb" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:16, 31 December 2014

OCA: New page: '''Unreleased structure''' The entry 4rzb is ON HOLD Authors: Fedorov, A.A., Fedorov, E.V., Marti-Arbona, R., Raushel, F.M., Almo, S.C. Description: The structure of N-formimino-L-Glut...

OCA — Wed, 31 Dec 2014 12:16:20 GMT

New page: '''Unreleased structure''' The entry 4rzb is ON HOLD Authors: Fedorov, A.A., Fedorov, E.V., Marti-Arbona, R., Raushel, F.M., Almo, S.C. Description: The structure of N-formimino-L-Glut...

New page

'''Unreleased structure'''

The entry 4rzb is ON HOLD

Authors: Fedorov, A.A., Fedorov, E.V., Marti-Arbona, R., Raushel, F.M., Almo, S.C.

Description: The structure of N-formimino-L-Glutamate Iminohydrolase from Pseudomonas aeruginosa complexed with N-formimino-L-Aspartate, SOAKED WITH MERCURY
[[Category: Unreleased Structures]]
[[Category: Almo, S.C]]
[[Category: Fedorov, A.A]]
[[Category: Marti-Arbona, R]]
[[Category: Fedorov, E.V]]
[[Category: Raushel, F.M]]