4s11 - Revision history

OCA at 10:38, 15 March 2023

OCA — Wed, 15 Mar 2023 10:38:27 GMT

←Older revision		Revision as of 10:38, 15 March 2023
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	<StructureSection load='4s11' size='340' side='right'caption='[[4s11]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4s11' size='340' side='right'caption='[[4s11]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4s11]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Camelus_glama Camelus~~ glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S11 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4S11 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4s11]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S11 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4S11 FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4s10\|4s10]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4s11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s11 OCA], [https://pdbe.org/4s11 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4s11 RCSB], [https://www.ebi.ac.uk/pdbsum/4s11 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4s11 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4s11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s11 OCA], [~~http~~://pdbe.org/4s11 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4s11 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4s11 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4s11 ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Camelus~~ glama]]	+	[[Category: Lama glama]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gettemans, J]]	+	[[Category: Gettemans J]]
-	[[Category: Loonchanta, A]]	+	[[Category: Loonchanta A]]
-	[[Category: Robinson~~, R C~~]]	+	[[Category: Robinson RC]]
-	[[Category: Wongsantichon, J]]	+	[[Category: Wongsantichon J]]
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Nanobody~~]]	+

OCA at 10:26, 1 May 2019

OCA — Wed, 01 May 2019 10:26:33 GMT

←Older revision		Revision as of 10:26, 1 May 2019
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	==Gelsolin nanobody shielding mutant plasma gelsolin from furin proteolysis==		==Gelsolin nanobody shielding mutant plasma gelsolin from furin proteolysis==
-	<StructureSection load='4s11' size='340' side='right' caption='[[4s11]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='4s11' size='340' side='right'caption='[[4s11]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4s11]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S11 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4S11 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4s11]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S11 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4S11 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4s10\|4s10]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4s10\|4s10]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4s11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s11 OCA], [http://pdbe.org/4s11 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4s11 RCSB], [http://www.ebi.ac.uk/pdbsum/4s11 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4s11 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4s11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s11 OCA], [http://pdbe.org/4s11 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4s11 RCSB], [http://www.ebi.ac.uk/pdbsum/4s11 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4s11 ProSAT]</span></td></tr>
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Camelus glama]]
		+	[[Category: Large Structures]]
	[[Category: Gettemans, J]]		[[Category: Gettemans, J]]
	[[Category: Loonchanta, A]]		[[Category: Loonchanta, A]]

OCA at 01:54, 10 March 2017

OCA — Fri, 10 Mar 2017 01:54:27 GMT

←Older revision		Revision as of 01:54, 10 March 2017
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		+
	==Gelsolin nanobody shielding mutant plasma gelsolin from furin proteolysis==		==Gelsolin nanobody shielding mutant plasma gelsolin from furin proteolysis==
	<StructureSection load='4s11' size='340' side='right' caption='[[4s11]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4s11' size='340' side='right' caption='[[4s11]], [[Resolution\|resolution]] 2.00Å' scene=''>
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	<table><tr><td colspan='2'>[[4s11]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S11 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4S11 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4s11]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S11 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4S11 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4s10\|4s10]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4s10\|4s10]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4s11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s11 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4s11 RCSB], [http://www.ebi.ac.uk/pdbsum/4s11 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4s11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s11 OCA], [http://pdbe.org/4s11 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4s11 RCSB], [http://www.ebi.ac.uk/pdbsum/4s11 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4s11 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4s11" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:32, 3 June 2015

OCA — Wed, 03 Jun 2015 12:32:53 GMT

←Older revision		Revision as of 12:32, 3 June 2015
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-	'''~~Unreleased~~ structure'''	+	==Gelsolin nanobody shielding mutant plasma gelsolin from furin proteolysis==
		+	<StructureSection load='4s11' size='340' side='right' caption='[[4s11]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4s11]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S11 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4S11 FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4s10\|4s10]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4s11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s11 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4s11 RCSB], [http://www.ebi.ac.uk/pdbsum/4s11 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Hereditary gelsolin amyloidosis is an autosomal dominantly inherited amyloid disorder. A point mutation in the GSN gene (G654A being the most common one) results in disturbed calcium binding by the second gelsolin domain (G2). As a result, the folding of G2 is hampered, rendering the mutant plasma gelsolin susceptible to a proteolytic cascade. Consecutive cleavage by furin and MT1-MMP-like proteases generates 8 and 5 kDa amyloidogenic peptides that cause neurological, ophthalmological and dermatological findings. To this day, no specific treatment is available to counter the pathogenesis. Using GSN nanobody 11 as a molecular chaperone, we aimed to protect mutant plasma gelsolin from furin proteolysis in the trans-Golgi network. We report a transgenic, GSN nanobody 11 secreting mouse that was used for crossbreeding with gelsolin amyloidosis mice. Insertion of the therapeutic nanobody gene into the gelsolin amyloidosis mouse genome resulted in improved muscle contractility. X-ray crystal structure determination of the gelsolin G2:Nb11 complex revealed that Nb11 does not directly block the furin cleavage site. We conclude that nanobodies can be used to shield substrates from aberrant proteolysis and this approach might establish a novel therapeutic strategy in amyloid diseases.

-	~~The entry 4s11 is ON HOLD until Paper Publication~~	+	An ER-directed gelsolin nanobody targets the first step in amyloid formation in a gelsolin amyloidosis mouse model.,Van Overbeke W, Wongsantichon J, Everaert I, Verhelle A, Zwaenepoel O, Loonchanta A, Burtnick LD, De Ganck A, Hochepied T, Haigh J, Cuvelier C, Derave W, Robinson RC, Gettemans J Hum Mol Genet. 2015 May 1;24(9):2492-507. doi: 10.1093/hmg/ddv010. Epub 2015 Jan , 18. PMID:25601851<ref>PMID:25601851</ref>

-	~~Authors: Wongsantichon~~, J.~~, Loonchanta, A~~.~~, Robinson, R.C., Gettemans, J~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Gelsolin nanobody shielding mutant plasma gelsolin from furin proteolysis~~	+	== References ==
-	~~[[Category: Unreleased Structures]]~~	+	<references/>
-	~~[[Category: Wongsantichon, J]]~~	+	__TOC__
		+	</StructureSection>
	[[Category: Gettemans, J]]		[[Category: Gettemans, J]]
-	[[Category: Robinson, R.C]]
	[[Category: Loonchanta, A]]		[[Category: Loonchanta, A]]
		+	[[Category: Robinson, R C]]
		+	[[Category: Wongsantichon, J]]
		+	[[Category: Immune system]]
		+	[[Category: Nanobody]]

OCA at 12:50, 7 February 2015

OCA — Sat, 07 Feb 2015 12:50:55 GMT

←Older revision		Revision as of 12:50, 7 February 2015
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4s11 is ON HOLD	+	The entry 4s11 is ON HOLD until Paper Publication

	Authors: Wongsantichon, J., Loonchanta, A., Robinson, R.C., Gettemans, J.		Authors: Wongsantichon, J., Loonchanta, A., Robinson, R.C., Gettemans, J.

OCA: Protected "4s11" [edit=sysop:move=sysop]

OCA — Wed, 14 Jan 2015 10:59:14 GMT

Protected "4s11" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:59, 14 January 2015

OCA: New page: '''Unreleased structure''' The entry 4s11 is ON HOLD Authors: Wongsantichon, J., Loonchanta, A., Robinson, R.C., Gettemans, J. Description: Gelsolin nanobody shielding mutant plasma ge...

OCA — Wed, 14 Jan 2015 10:59:13 GMT

New page: '''Unreleased structure''' The entry 4s11 is ON HOLD Authors: Wongsantichon, J., Loonchanta, A., Robinson, R.C., Gettemans, J. Description: Gelsolin nanobody shielding mutant plasma ge...

New page

'''Unreleased structure'''

The entry 4s11 is ON HOLD

Authors: Wongsantichon, J., Loonchanta, A., Robinson, R.C., Gettemans, J.

Description: Gelsolin nanobody shielding mutant plasma gelsolin from furin proteolysis
[[Category: Unreleased Structures]]
[[Category: Wongsantichon, J]]
[[Category: Gettemans, J]]
[[Category: Robinson, R.C]]
[[Category: Loonchanta, A]]