4s3g - Revision history

OCA at 10:42, 15 March 2023

OCA — Wed, 15 Mar 2023 10:42:50 GMT

←Older revision		Revision as of 10:42, 15 March 2023
Line 3:		Line 3:
	<StructureSection load='4s3g' size='340' side='right'caption='[[4s3g]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4s3g' size='340' side='right'caption='[[4s3g]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4s3g]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Staae Staae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S3G OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4S3G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4s3g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_str._Newman Staphylococcus aureus subsp. aureus str. Newman]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S3G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4S3G FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=INS:1,2,3,4,5,6-HEXAHYDROXY-CYCLOHEXANE'>INS</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=INS:1,2,3,4,5,6-HEXAHYDROXY-CYCLOHEXANE'>INS</scene>, <scene name='pdbligand=PF5:2,3,4,5,6-PENTAFLUORO-L-PHENYLALANINE'>PF5</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=PF5:2,3,4,5,6-PENTAFLUORO-L-PHENYLALANINE'>PF5</scene~~></td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4s3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s3g OCA], [https://pdbe.org/4s3g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4s3g RCSB], [https://www.ebi.ac.uk/pdbsum/4s3g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4s3g ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4rv3\|4rv3]]</td></tr>~~	+
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">plc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=426430 STAAE])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol_diacylglycerol-lyase Phosphatidylinositol diacylglycerol-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.13 4.6.1.13] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4s3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s3g OCA], [~~http~~://pdbe.org/4s3g PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4s3g RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4s3g PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4s3g ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/PLC_STAAE PLC_STAAE]] Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates. Potential virulence factor.	+	[https://www.uniprot.org/uniprot/PLC_STAAE PLC_STAAE] Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates. Potential virulence factor.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 30:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Phosphatidylinositol diacylglycerol-lyase]]~~	+	[[Category: Staphylococcus aureus subsp. aureus str. Newman]]
-	~~[[Category: Staae~~]]	+	[[Category: Eyles SJ]]
-	[[Category: Eyles~~, S J~~]]	+	[[Category: Gao J]]
-	[[Category: Gao, J]]	+	[[Category: Gershenson A]]
-	[[Category: Gershenson, A]]	+	[[Category: He T]]
-	[[Category: He, T]]	+	[[Category: Roberts MF]]
-	[[Category: Roberts~~, M F]]~~	+
-	~~[[Category: Cation-pi]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Phospholipase]]~~	+
-	~~[[Category: Tim barrel~~]]	+

OCA at 09:40, 1 May 2019

OCA — Wed, 01 May 2019 09:40:03 GMT

←Older revision		Revision as of 09:40, 1 May 2019
Line 1:		Line 1:

	==Structure of the F249X mutant of Phosphatidylinositol-specific phospholipase C from Staphylococcus aureus==		==Structure of the F249X mutant of Phosphatidylinositol-specific phospholipase C from Staphylococcus aureus==
-	<StructureSection load='4s3g' size='340' side='right' caption='[[4s3g]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='4s3g' size='340' side='right'caption='[[4s3g]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4s3g]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S3G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4S3G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4s3g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staae Staae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S3G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4S3G FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=INS:1,2,3,4,5,6-HEXAHYDROXY-CYCLOHEXANE'>INS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=INS:1,2,3,4,5,6-HEXAHYDROXY-CYCLOHEXANE'>INS</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PF5:2,3,4,5,6-PENTAFLUORO-L-PHENYLALANINE'>PF5</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PF5:2,3,4,5,6-PENTAFLUORO-L-PHENYLALANINE'>PF5</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4rv3\|4rv3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4rv3\|4rv3]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">plc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=426430 STAAE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol_diacylglycerol-lyase Phosphatidylinositol diacylglycerol-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.13 4.6.1.13] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol_diacylglycerol-lyase Phosphatidylinositol diacylglycerol-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.13 4.6.1.13] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4s3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s3g OCA], [http://pdbe.org/4s3g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4s3g RCSB], [http://www.ebi.ac.uk/pdbsum/4s3g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4s3g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4s3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s3g OCA], [http://pdbe.org/4s3g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4s3g RCSB], [http://www.ebi.ac.uk/pdbsum/4s3g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4s3g ProSAT]</span></td></tr>
Line 28:		Line 29:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Phosphatidylinositol diacylglycerol-lyase]]		[[Category: Phosphatidylinositol diacylglycerol-lyase]]
		+	[[Category: Staae]]
	[[Category: Eyles, S J]]		[[Category: Eyles, S J]]
	[[Category: Gao, J]]		[[Category: Gao, J]]

OCA at 14:13, 24 May 2017

OCA — Wed, 24 May 2017 14:13:00 GMT

←Older revision		Revision as of 14:13, 24 May 2017
Line 1:		Line 1:
		+
	==Structure of the F249X mutant of Phosphatidylinositol-specific phospholipase C from Staphylococcus aureus==		==Structure of the F249X mutant of Phosphatidylinositol-specific phospholipase C from Staphylococcus aureus==
	<StructureSection load='4s3g' size='340' side='right' caption='[[4s3g]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4s3g' size='340' side='right' caption='[[4s3g]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 7:		Line 8:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4rv3\|4rv3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4rv3\|4rv3]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol_diacylglycerol-lyase Phosphatidylinositol diacylglycerol-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.13 4.6.1.13] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol_diacylglycerol-lyase Phosphatidylinositol diacylglycerol-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.13 4.6.1.13] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4s3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s3g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4s3g RCSB], [http://www.ebi.ac.uk/pdbsum/4s3g PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4s3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s3g OCA], [http://pdbe.org/4s3g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4s3g RCSB], [http://www.ebi.ac.uk/pdbsum/4s3g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4s3g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4s3g" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Phospholipase C\|Phospholipase C]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:37, 15 July 2015

OCA — Wed, 15 Jul 2015 07:37:21 GMT

←Older revision		Revision as of 07:37, 15 July 2015
Line 11:		Line 11:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/PLC_STAAE PLC_STAAE]] Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates. Potential virulence factor.		[[http://www.uniprot.org/uniprot/PLC_STAAE PLC_STAAE]] Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates. Potential virulence factor.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cation-pi interactions, where protein aromatic residues supply pi systems while positive charged portion of phospholipid head groups are the cations, have been suggested as important binding modes for peripheral membrane proteins. However, aromatic amino acids can also insert into membranes and hydrophobically interact with lipid tails. Heretofore there has been no facile way to differentiate these two types of interactions. We show that specific incorporation of fluorinated amino acids into proteins can experimentally distinguish cation-pi interactions from membrane insertion of the aromatic side-chains. Fluorinated aromatic amino acids destabilize the cation-pi interactions by altering electrostatics of the aromatic ring while their increased hydrophobicity enhances membrane insertion. Incorporation of pentafluorophenylalanine or difluorotyrosine into a Staphylococcus aureus phosphatidylinositol-specific phospholipase C (PI-PLC) variant engineered to contain a specific PC-binding site demonstrates the effectiveness of this methodology. Applying this methodology to the plethora of tyrosine residues in Bacillus thuringiensis PI-PLC definitively identifies those involved in cation-pi interactions with PC. This powerful method can easily be used to determine the roles of aromatic residues in other peripheral membrane proteins and in integral membrane proteins.
		+
		+	Fluorinated aromatic amino acids distinguish cation-pi interactions from membrane insertion.,He T, Gershenson A, Eyles SJ, Lee YJ, Liu WR, Wang J, Gao J, Roberts MF J Biol Chem. 2015 Jun 19. pii: jbc.M115.668343. PMID:26092728<ref>PMID:26092728</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 12:20, 1 July 2015

OCA — Wed, 01 Jul 2015 12:20:09 GMT

←Older revision		Revision as of 12:20, 1 July 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Structure of the F249X mutant of Phosphatidylinositol-specific phospholipase C from Staphylococcus aureus==
-		+	<StructureSection load='4s3g' size='340' side='right' caption='[[4s3g]], [[Resolution\|resolution]] 2.50Å' scene=''>
-	~~The entry~~ 4s3g is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4s3g]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S3G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4S3G FirstGlance]. <br>
-	~~Authors~~: He, T., ~~Gershenson~~, A., ~~Eyles~~, S.J., ~~Gao, J~~., ~~Roberts, M~~.F.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=INS:1,2,3,4,5,6-HEXAHYDROXY-CYCLOHEXANE'>INS</scene></td></tr>
-		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PF5:2,3,4,5,6-PENTAFLUORO-L-PHENYLALANINE'>PF5</scene></td></tr>
-	~~Description~~: ~~Structure of the F249X mutant of~~ Phosphatidylinositol-~~specific phospholipase C from Staphylococcus aureus~~	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4rv3\|4rv3]]</td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol_diacylglycerol-lyase Phosphatidylinositol diacylglycerol-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.13 4.6.1.13] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4s3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s3g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4s3g RCSB], [http://www.ebi.ac.uk/pdbsum/4s3g PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PLC_STAAE PLC_STAAE]] Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates. Potential virulence factor.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Phosphatidylinositol diacylglycerol-lyase]]
		+	[[Category: Eyles, S J]]
		+	[[Category: Gao, J]]
	[[Category: Gershenson, A]]		[[Category: Gershenson, A]]
-	[[Category: Roberts, M.F]]
	[[Category: He, T]]		[[Category: He, T]]
-	[[Category: ~~Eyles~~, ~~S.J~~]]	+	[[Category: Roberts, M F]]
-	[[Category: ~~Gao, J~~]]	+	[[Category: Cation-pi]]
		+	[[Category: Lyase]]
		+	[[Category: Phospholipase]]
		+	[[Category: Tim barrel]]

OCA at 08:46, 12 February 2015

OCA — Thu, 12 Feb 2015 08:46:52 GMT

←Older revision		Revision as of 08:46, 12 February 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4s3g is ON HOLD	+	The entry 4s3g is ON HOLD until Paper Publication

	Authors: He, T., Gershenson, A., Eyles, S.J., Gao, J., Roberts, M.F.		Authors: He, T., Gershenson, A., Eyles, S.J., Gao, J., Roberts, M.F.

OCA: Protected "4s3g" [edit=sysop:move=sysop]

OCA — Sat, 07 Feb 2015 12:55:19 GMT

Protected "4s3g" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:55, 7 February 2015

OCA: New page: '''Unreleased structure''' The entry 4s3g is ON HOLD Authors: He, T., Gershenson, A., Eyles, S.J., Gao, J., Roberts, M.F. Description: Structure of the F249X mutant of Phosphatidylinos...

OCA — Sat, 07 Feb 2015 12:55:18 GMT

New page: '''Unreleased structure''' The entry 4s3g is ON HOLD Authors: He, T., Gershenson, A., Eyles, S.J., Gao, J., Roberts, M.F. Description: Structure of the F249X mutant of Phosphatidylinos...

New page

'''Unreleased structure'''

The entry 4s3g is ON HOLD

Authors: He, T., Gershenson, A., Eyles, S.J., Gao, J., Roberts, M.F.

Description: Structure of the F249X mutant of Phosphatidylinositol-specific phospholipase C from Staphylococcus aureus
[[Category: Unreleased Structures]]
[[Category: Gershenson, A]]
[[Category: Roberts, M.F]]
[[Category: He, T]]
[[Category: Eyles, S.J]]
[[Category: Gao, J]]