4sgb - Revision history

OCA at 10:21, 1 May 2019

OCA — Wed, 01 May 2019 10:21:52 GMT

←Older revision		Revision as of 10:21, 1 May 2019
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	==STRUCTURE OF THE COMPLEX OF STREPTOMYCES GRISEUS PROTEINASE B AND POLYPEPTIDE CHYMOTRYPSIN INHIBITOR-1 FROM RUSSET BURBANK POTATO TUBERS AT 2.1 ANGSTROMS RESOLUTION==		==STRUCTURE OF THE COMPLEX OF STREPTOMYCES GRISEUS PROTEINASE B AND POLYPEPTIDE CHYMOTRYPSIN INHIBITOR-1 FROM RUSSET BURBANK POTATO TUBERS AT 2.1 ANGSTROMS RESOLUTION==
-	<StructureSection load='4sgb' size='340' side='right' caption='[[4sgb]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='4sgb' size='340' side='right'caption='[[4sgb]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4sgb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_griseus"_krainsky_1914 "actinomyces griseus" krainsky 1914]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4SGB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4SGB FirstGlance]. <br>		<table><tr><td colspan='2'>[[4sgb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_griseus"_krainsky_1914 "actinomyces griseus" krainsky 1914]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4SGB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4SGB FirstGlance]. <br>
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sg/4sgb_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sg/4sgb_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</StructureSection>		</StructureSection>
	[[Category: Actinomyces griseus krainsky 1914]]		[[Category: Actinomyces griseus krainsky 1914]]
		+	[[Category: Large Structures]]
	[[Category: Greenblatt, H]]		[[Category: Greenblatt, H]]
	[[Category: James, M]]		[[Category: James, M]]

OCA at 02:09, 5 August 2016

OCA — Fri, 05 Aug 2016 02:09:02 GMT

←Older revision		Revision as of 02:09, 5 August 2016
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		+
	==STRUCTURE OF THE COMPLEX OF STREPTOMYCES GRISEUS PROTEINASE B AND POLYPEPTIDE CHYMOTRYPSIN INHIBITOR-1 FROM RUSSET BURBANK POTATO TUBERS AT 2.1 ANGSTROMS RESOLUTION==		==STRUCTURE OF THE COMPLEX OF STREPTOMYCES GRISEUS PROTEINASE B AND POLYPEPTIDE CHYMOTRYPSIN INHIBITOR-1 FROM RUSSET BURBANK POTATO TUBERS AT 2.1 ANGSTROMS RESOLUTION==
	<StructureSection load='4sgb' size='340' side='right' caption='[[4sgb]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='4sgb' size='340' side='right' caption='[[4sgb]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4sgb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_griseus Streptomyces~~ griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4SGB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4SGB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4sgb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_griseus"_krainsky_1914 "actinomyces griseus" krainsky 1914]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4SGB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4SGB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4sgb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4sgb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4sgb RCSB], [http://www.ebi.ac.uk/pdbsum/4sgb PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4sgb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4sgb OCA], [http://pdbe.org/4sgb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4sgb RCSB], [http://www.ebi.ac.uk/pdbsum/4sgb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4sgb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4sgb ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4sgb" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Streptomyces~~ griseus]]	+	[[Category: Actinomyces griseus krainsky 1914]]
	[[Category: Greenblatt, H]]		[[Category: Greenblatt, H]]
	[[Category: James, M]]		[[Category: James, M]]

OCA at 14:57, 25 December 2014

OCA — Thu, 25 Dec 2014 14:57:05 GMT

←Older revision		Revision as of 14:57, 25 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4sgb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4sgb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4sgb RCSB], [http://www.ebi.ac.uk/pdbsum/4sgb PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4sgb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4sgb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4sgb RCSB], [http://www.ebi.ac.uk/pdbsum/4sgb PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PRTB_STRGR PRTB_STRGR]] Has a primary specificity for large aliphatic or aromatic amino acids. [[http://www.uniprot.org/uniprot/IP2K_SOLTU IP2K_SOLTU]] Inhibitor of trypsin and chymotrypsin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:39, 18 December 2014

OCA — Thu, 18 Dec 2014 08:39:48 GMT

←Older revision		Revision as of 08:39, 18 December 2014
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-	~~{{STRUCTURE_4sgb\| PDB=4sgb \| SCENE= }}~~	+	==STRUCTURE OF THE COMPLEX OF STREPTOMYCES GRISEUS PROTEINASE B AND POLYPEPTIDE CHYMOTRYPSIN INHIBITOR-1 FROM RUSSET BURBANK POTATO TUBERS AT 2.1 ANGSTROMS RESOLUTION==
-	===STRUCTURE OF THE COMPLEX OF STREPTOMYCES GRISEUS PROTEINASE B AND POLYPEPTIDE CHYMOTRYPSIN INHIBITOR-1 FROM RUSSET BURBANK POTATO TUBERS AT 2.1 ANGSTROMS RESOLUTION===	+	<StructureSection load='4sgb' size='340' side='right' caption='[[4sgb]], [[Resolution\|resolution]] 2.10Å' scene=''>
-	~~{{ABSTRACT_PUBMED_2494344}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4sgb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4SGB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4SGB FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4sgb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4sgb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4sgb RCSB], [http://www.ebi.ac.uk/pdbsum/4sgb PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sg/4sgb_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A low molecular weight protein inhibitor of serine proteinases from Russet Burbank potato tubers, polypeptide chymotrypsin inhibitor-1 (PCI-1), has been crystallized in complex with Streptomyces griseus proteinase B (SGPB). The three-dimensional structure of the complex has been solved at 2.1 A resolution by the molecular replacement method and has been refined to a final R-factor (= sigma[[Fo[-[Fc[[/sigma[Fo[) of 0.142 (8.0 to 2.1 A resolution data). The reactive site bond of PCI-1 (Leu38I to Asn39I) is intact in the complex, and there is no significant distortion of the peptide from planarity. The distance between the active site serine O gamma of SGPB and the carbonyl carbon of the scissile bond of PCI-1 is 2.8 A (1 A = 0.1 nm). The inhibitor has little secondary structure, having a three-stranded antiparallel beta-sheet on the side opposite the reactive site and four beta-turns. PCI-1 has four disulphide bridges; these presumably take the place of extensive secondary structure in keeping the reactive site conformationally constrained. The pairing of the cystine residues, which had not been characterized chemically, is as follows: Cys3I to Cys40I, Cys6I to Cys24I, Cys7I to Cys36I, and Cys13I to Cys49I. The molecular structure of SGPB in the PCI-1 complex agrees closely with the structure of SGPB complexed with the third domain of the turkey ovomucoid inhibitor (OMTKY3). A least-squares overlap of all atoms in SGPB gives a root-mean-square difference of 0.37 A. One of the loops of SGPB (Ser35 to Gly40) differs in conformation in the two complexes by more than 2.0 A root-mean-square for the main-chain atoms. Thr39 displays the largest differences with the carbonyl carbon atom deviating by 3.6 A. This conformational alternative is a result of the differences in the molecular structures of the P'4 residues following the reactive site bonds of the two inhibitors. This displacement avoids a close contact (1.3 A) between the carbonyl oxygen of Ser38 of SGPB and Pro42I C beta of PCI-1. The solvent structure of the PCI-1-SGPB complex includes 179 waters, two sulphate or phosphate ions, and one calcium or potassium ion, which appears to play a role in crystal formation. The molecular structure of PCI-1 determined here has allowed the proposal of a model for the structure of a two-domain inhibitor from potatoes and tomatoes, inhibitor II.(ABSTRACT TRUNCATED AT 400 WORDS)

-	~~==About this~~ Structure==	+	Structure of the complex of Streptomyces griseus proteinase B and polypeptide chymotrypsin inhibitor-1 from Russet Burbank potato tubers at 2.1 A resolution.,Greenblatt HM, Ryan CA, James MN J Mol Biol. 1989 Jan 5;205(1):201-28. PMID:2494344<ref>PMID:2494344</ref>
-	~~[[4sgb]] is a~~ 2 ~~chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptomyces_griseus Streptomyces griseus]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4SGB OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
		+	*[[Proteinase\|Proteinase]]
	*[[Streptomyces griseus proteinase B\|Streptomyces griseus proteinase B]]		*[[Streptomyces griseus proteinase B\|Streptomyces griseus proteinase B]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:002494344<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Streptomyces griseus]]		[[Category: Streptomyces griseus]]
-	[[Category: Greenblatt, H.]]	+	[[Category: Greenblatt, H]]
-	[[Category: James, M.]]	+	[[Category: James, M]]

OCA at 11:07, 11 March 2013

OCA — Mon, 11 Mar 2013 11:07:16 GMT

←Older revision		Revision as of 11:07, 11 March 2013
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-	[[Image:4sgb.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_4sgb", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_4sgb\| PDB=4sgb \| SCENE= }}		{{STRUCTURE_4sgb\| PDB=4sgb \| SCENE= }}
-
	===STRUCTURE OF THE COMPLEX OF STREPTOMYCES GRISEUS PROTEINASE B AND POLYPEPTIDE CHYMOTRYPSIN INHIBITOR-1 FROM RUSSET BURBANK POTATO TUBERS AT 2.1 ANGSTROMS RESOLUTION===		===STRUCTURE OF THE COMPLEX OF STREPTOMYCES GRISEUS PROTEINASE B AND POLYPEPTIDE CHYMOTRYPSIN INHIBITOR-1 FROM RUSSET BURBANK POTATO TUBERS AT 2.1 ANGSTROMS RESOLUTION===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_2494344}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 2494344 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_2494344}}		{{ABSTRACT_PUBMED_2494344}}

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	==See Also==		==See Also==
	*[[Streptomyces griseus proteinase B\|Streptomyces griseus proteinase B]]		*[[Streptomyces griseus proteinase B\|Streptomyces griseus proteinase B]]
-	*[[User:Harry Greenblatt\|User:Harry Greenblatt]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:002494344~~</ref><ref group="xtra">PMID:012215419~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:002494344</ref><references group="xtra"/>
	[[Category: Streptomyces griseus]]		[[Category: Streptomyces griseus]]
	[[Category: Greenblatt, H.]]		[[Category: Greenblatt, H.]]
	[[Category: James, M.]]		[[Category: James, M.]]

OCA: Protected "4sgb" [edit=sysop:move=sysop]

OCA — Thu, 14 Jun 2012 10:06:03 GMT

Protected "4sgb" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:06, 14 June 2012