4sli - Revision history

OCA at 12:58, 1 March 2024

OCA — Fri, 01 Mar 2024 12:58:07 GMT

←Older revision		Revision as of 12:58, 1 March 2024
Line 1:		Line 1:
		+
	==LEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH 2-PROPENYL-NEU5AC, AN INACTIVE SUBSTRATE ANALOGUE==		==LEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH 2-PROPENYL-NEU5AC, AN INACTIVE SUBSTRATE ANALOGUE==
-	<StructureSection load='4sli' size='340' side='right' caption='[[4sli]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='4sli' size='340' side='right'caption='[[4sli]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4sli]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Macde Macde~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4SLI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4SLI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4sli]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Macrobdella_decora Macrobdella decora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4SLI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4SLI FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=CNP:2-PROPENYL-N-ACETYL-NEURAMIC+ACID'>CNP</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">T7 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=6405 MACDE])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CNP:2-PROPENYL-N-ACETYL-NEURAMIC+ACID'>CNP</scene></td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks'>[http~~:~~//en.wikipedia.org/wiki/Exo~~-~~alpha~~-~~sialidase Exo~~-~~alpha~~-~~sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4sli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4sli OCA], [https://pdbe.org/4sli PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4sli RCSB], [https://www.ebi.ac.uk/pdbsum/4sli PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4sli ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4sli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4sli OCA], [~~http~~://pdbe.org/4sli PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4sli RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4sli PDBsum]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/NANL_MACDE NANL_MACDE]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sl/4sli_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sl/4sli_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 18:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4sli ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4sli ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Intramolecular trans-sialidase from leech (Macrobdella decora) is the first member of the sialidase superfamily found to exhibit strict specificity towards the cleavage of terminal Neu5Acalpha2-->3Gal linkage in sialoglycoconjugates. Its release of 2,7-anhydro-Neu5Ac instead of Neu5Ac indicates that it catalyzes an intramolecular trans-sialosyl reaction. Crystal structures of its complexes with an inactive substrate analogue 2-propenyl-Neu5Ac, and with the product 2,7-anhydro-Neu5Ac, have been determined to 1.8 A resolution. The boat conformation of the pyranose observed in the complexes supports the proposed enzymatic mechanism that O7 of an axial 6-glycerol group attacks the positively charged C2 of the intermediate. A generalized mechanism is proposed for the sialidase superfamily.
-
-	The 1.8 A structures of leech intramolecular trans-sialidase complexes: evidence of its enzymatic mechanism.,Luo Y, Li SC, Li YT, Luo M J Mol Biol. 1999 Jan 8;285(1):323-32. PMID:9878409<ref>PMID:9878409</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4sli" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Exo-alpha-sialidase~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Macde~~]]	+	[[Category: Macrobdella decora]]
-	[[Category: Li~~, S C~~]]	+	[[Category: Li SC]]
-	[[Category: Li~~, Y T~~]]	+	[[Category: Li YT]]
-	[[Category: Luo, M]]	+	[[Category: Luo M]]
-	[[Category: Luo, Y]]	+	[[Category: Luo Y]]
-	~~[[Category: Enzyme]]~~	+
-	~~[[Category: Intramolecular trans-sialidase~~]]	+

OCA at 07:26, 8 February 2016

OCA — Mon, 08 Feb 2016 07:26:23 GMT

←Older revision		Revision as of 07:26, 8 February 2016
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6405 MACDE])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6405 MACDE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4sli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4sli OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4sli RCSB], [http://www.ebi.ac.uk/pdbsum/4sli PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4sli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4sli OCA], [http://pdbe.org/4sli PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4sli RCSB], [http://www.ebi.ac.uk/pdbsum/4sli PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4sli ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4sli" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:20, 5 January 2015

OCA — Mon, 05 Jan 2015 09:20:45 GMT

←Older revision		Revision as of 09:20, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4sli]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Macde Macde]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4SLI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4SLI FirstGlance]. <br>		<table><tr><td colspan='2'>[[4sli]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Macde Macde]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4SLI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4SLI FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CNP:2-PROPENYL-N-ACETYL-NEURAMIC+ACID'>CNP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CNP:2-PROPENYL-N-ACETYL-NEURAMIC+ACID'>CNP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6405 MACDE])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6405 MACDE])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4sli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4sli OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4sli RCSB], [http://www.ebi.ac.uk/pdbsum/4sli PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4sli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4sli OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4sli RCSB], [http://www.ebi.ac.uk/pdbsum/4sli PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	[[Category: Exo-alpha-sialidase]]		[[Category: Exo-alpha-sialidase]]
	[[Category: Macde]]		[[Category: Macde]]
-	[[Category: Li, S C.]]	+	[[Category: Li, S C]]
-	[[Category: Li, Y T.]]	+	[[Category: Li, Y T]]
-	[[Category: Luo, M.]]	+	[[Category: Luo, M]]
-	[[Category: Luo, Y.]]	+	[[Category: Luo, Y]]
	[[Category: Enzyme]]		[[Category: Enzyme]]
	[[Category: Intramolecular trans-sialidase]]		[[Category: Intramolecular trans-sialidase]]

OCA: Protected "4sli" [edit=sysop:move=sysop]

OCA — Thu, 05 Jun 2014 07:32:55 GMT

Protected "4sli" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:32, 5 June 2014

OCA at 07:32, 5 June 2014

OCA — Thu, 05 Jun 2014 07:32:53 GMT

←Older revision		Revision as of 07:32, 5 June 2014
Line 1:		Line 1:
-	~~{{Seed}}~~	+	==LEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH 2-PROPENYL-NEU5AC, AN INACTIVE SUBSTRATE ANALOGUE==
-	[[~~Image:~~4sli.~~png~~\|~~left~~\|200px]]	+	<StructureSection load='4sli' size='340' side='right' caption='[[4sli]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4sli]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Macde Macde]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4SLI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4SLI FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CNP:2-PROPENYL-N-ACETYL-NEURAMIC+ACID'>CNP</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6405 MACDE])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4sli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4sli OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4sli RCSB], [http://www.ebi.ac.uk/pdbsum/4sli PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sl/4sli_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Intramolecular trans-sialidase from leech (Macrobdella decora) is the first member of the sialidase superfamily found to exhibit strict specificity towards the cleavage of terminal Neu5Acalpha2-->3Gal linkage in sialoglycoconjugates. Its release of 2,7-anhydro-Neu5Ac instead of Neu5Ac indicates that it catalyzes an intramolecular trans-sialosyl reaction. Crystal structures of its complexes with an inactive substrate analogue 2-propenyl-Neu5Ac, and with the product 2,7-anhydro-Neu5Ac, have been determined to 1.8 A resolution. The boat conformation of the pyranose observed in the complexes supports the proposed enzymatic mechanism that O7 of an axial 6-glycerol group attacks the positively charged C2 of the intermediate. A generalized mechanism is proposed for the sialidase superfamily.

-	~~<!--~~	+	The 1.8 A structures of leech intramolecular trans-sialidase complexes: evidence of its enzymatic mechanism.,Luo Y, Li SC, Li YT, Luo M J Mol Biol. 1999 Jan 8;285(1):323-32. PMID:9878409<ref>PMID:9878409</ref>
-	The ~~line below this paragraph~~, ~~containing "STRUCTURE_4sli"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_4sli\| PDB=4sli \| SCENE= }}~~	+

-	~~===LEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH 2-PROPENYL-NEU5AC~~, ~~AN INACTIVE SUBSTRATE ANALOGUE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_9878409}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 9878409 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_9878409}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~4SLI is a 1 chain structure of sequence from [http:~~/~~/en.wikipedia.org/wiki/Macrobdella_decora Macrobdella decora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4SLI OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:9878409<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Exo-alpha-sialidase]]		[[Category: Exo-alpha-sialidase]]
-	[[Category: ~~Macrobdella decora~~]]	+	[[Category: Macde]]
	[[Category: Li, S C.]]		[[Category: Li, S C.]]
	[[Category: Li, Y T.]]		[[Category: Li, Y T.]]
Line 32:		Line 38:
	[[Category: Enzyme]]		[[Category: Enzyme]]
	[[Category: Intramolecular trans-sialidase]]		[[Category: Intramolecular trans-sialidase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 20:11:08 2009''

OCA at 18:11, 16 February 2009

OCA — Mon, 16 Feb 2009 18:11:10 GMT

←Older revision		Revision as of 18:11, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	4SLI is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Macrobdella_decora Macrobdella decora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4SLI OCA].	+	4SLI is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Macrobdella_decora Macrobdella decora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4SLI OCA].

	==Reference==		==Reference==
-	~~The 1.8 A structures of leech intramolecular trans-sialidase complexes: evidence of its enzymatic mechanism., Luo Y, Li SC, Li YT, Luo M, J Mol Biol. 1999 Jan 8;285(1):323-32.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/9878409 9878409]~~	+	<ref group="xtra">PMID:9878409</ref><references group="xtra"/>
	[[Category: Exo-alpha-sialidase]]		[[Category: Exo-alpha-sialidase]]
	[[Category: Macrobdella decora]]		[[Category: Macrobdella decora]]
-	[[Category: Single protein]]
	[[Category: Li, S C.]]		[[Category: Li, S C.]]
	[[Category: Li, Y T.]]		[[Category: Li, Y T.]]
Line 34:		Line 33:
	[[Category: Intramolecular trans-sialidase]]		[[Category: Intramolecular trans-sialidase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu Jul 3 13~~:50:~~49 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 20:11:08 2009''

OCA at 10:50, 3 July 2008

OCA — Thu, 03 Jul 2008 10:50:50 GMT

←Older revision		Revision as of 10:50, 3 July 2008
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-	[[Image:4sli.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:4sli.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_4sli\| PDB=4sli \| SCENE= }}		{{STRUCTURE_4sli\| PDB=4sli \| SCENE= }}

-	~~'''~~LEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH 2-PROPENYL-NEU5AC, AN INACTIVE SUBSTRATE ANALOGUE~~'''~~	+	===LEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH 2-PROPENYL-NEU5AC, AN INACTIVE SUBSTRATE ANALOGUE===


-	~~==Overview==~~	+	<!--
-	~~Intramolecular trans~~-~~sialidase from leech (Macrobdella decora) is the first member of the sialidase superfamily found to exhibit strict specificity towards the cleavage of terminal Neu5Acalpha2~~-~~->3Gal linkage in sialoglycoconjugates. Its release of 2~~,~~7-anhydro-Neu5Ac instead of Neu5Ac indicates that it catalyzes an intramolecular trans-sialosyl reaction. Crystal structures of its complexes with an inactive substrate analogue 2-propenyl-Neu5Ac~~, ~~and with~~ the ~~product 2,7-anhydro-Neu5Ac, have been determined~~ to 1.~~8 A resolution~~. The boat conformation of the pyranose observed in the complexes supports the proposed enzymatic mechanism that O7 of an axial 6-glycerol group attacks the positively charged C2 of the intermediate. A generalized mechanism is ~~proposed for~~ the ~~sialidase superfamily~~.	+	The line below this paragraph, {{ABSTRACT_PUBMED_9878409}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 9878409 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_9878409}}

	==About this Structure==		==About this Structure==
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	[[Category: Enzyme]]		[[Category: Enzyme]]
	[[Category: Intramolecular trans-sialidase]]		[[Category: Intramolecular trans-sialidase]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May~~ ~~4 22~~:29:33 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:50:49 2008''

OCA at 19:29, 4 May 2008

OCA — Sun, 04 May 2008 19:29:34 GMT

←Older revision		Revision as of 19:29, 4 May 2008
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	[[Image:4sli.jpg\|left\|200px]]		[[Image:4sli.jpg\|left\|200px]]

-	~~{{Structure~~	+	<!--
-	~~\|PDB= 4sli \|SIZE=350\|CAPTION=~~ <~~scene name='initialview01'>4sli</scene>, resolution 1.8Å~~	+	The line below this paragraph, containing "STRUCTURE_4sli", creates the "Structure Box" on the page.
-	~~\|SITE=~~	+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	~~\|LIGAND= <scene name='pdbligand=CNP:2~~-~~PROPENYL~~-~~N-ACETYL-NEURAMIC+ACID'>CNP</scene>~~	+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	~~\|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase]~~, ~~with EC number [http://www~~.~~brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span>~~	+	or leave the SCENE parameter empty for the default display.
-	~~\|GENE= T7~~ (~~[http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6405 Macrobdella decora]~~)	+	-->
-	~~\|DOMAIN=~~	+	{{STRUCTURE_4sli\| PDB=4sli \| SCENE= }}
-	~~\|RELATEDENTRY=~~	+
-	~~\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca~~-~~docs/fgij/fg.htm?mol=4sli FirstGlance], [http://oca.weizmann.ac.il/oca~~-~~bin/ocaids?id~~=4sli ~~OCA], [http://www.ebi.ac.uk/pdbsum/4sli PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId~~=~~4sli RCSB]</span>~~	+
-	}}	+

	'''LEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH 2-PROPENYL-NEU5AC, AN INACTIVE SUBSTRATE ANALOGUE'''		'''LEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH 2-PROPENYL-NEU5AC, AN INACTIVE SUBSTRATE ANALOGUE'''
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	[[Category: Luo, M.]]		[[Category: Luo, M.]]
	[[Category: Luo, Y.]]		[[Category: Luo, Y.]]
-	[[Category: ~~enzyme~~]]	+	[[Category: Enzyme]]
-	[[Category: ~~intramolecular~~ trans-sialidase]]	+	[[Category: Intramolecular trans-sialidase]]
-		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:29:33 2008''
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Mar 31 05~~:40:10 2008''	+

OCA at 02:40, 31 March 2008

OCA — Mon, 31 Mar 2008 02:40:11 GMT

←Older revision		Revision as of 02:40, 31 March 2008
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	\|PDB= 4sli \|SIZE=350\|CAPTION= <scene name='initialview01'>4sli</scene>, resolution 1.8Å		\|PDB= 4sli \|SIZE=350\|CAPTION= <scene name='initialview01'>4sli</scene>, resolution 1.8Å
	\|SITE=		\|SITE=
-	\|LIGAND= <scene name='pdbligand=CNP:2-PROPENYL-N-ACETYL-NEURAMIC ACID'>CNP</scene>	+	\|LIGAND= <scene name='pdbligand=CNP:2-PROPENYL-N-ACETYL-NEURAMIC+ACID'>CNP</scene>
-	\|ACTIVITY= [http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18]	+	\|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] </span>
	\|GENE= T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6405 Macrobdella decora])		\|GENE= T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6405 Macrobdella decora])
		+	\|DOMAIN=
		+	\|RELATEDENTRY=
		+	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4sli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4sli OCA], [http://www.ebi.ac.uk/pdbsum/4sli PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=4sli RCSB]</span>
	}}		}}

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	[[Category: Luo, M.]]		[[Category: Luo, M.]]
	[[Category: Luo, Y.]]		[[Category: Luo, Y.]]
-	[[Category: CNP]]
	[[Category: enzyme]]		[[Category: enzyme]]
	[[Category: intramolecular trans-sialidase]]		[[Category: intramolecular trans-sialidase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu~~ Mar ~~20 19~~:10:57 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:40:10 2008''

OCA at 17:10, 20 March 2008

OCA — Thu, 20 Mar 2008 17:10:58 GMT

←Older revision		Revision as of 17:10, 20 March 2008
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-	[[Image:4sli.jpg\|left\|200px]]~~<br /><applet load~~="4sli~~" size~~="350~~" color~~=~~"white" frame~~=~~"true" align="right" spinBox="true"~~	+	[[Image:4sli.jpg\|left\|200px]]
-	~~caption="~~4sli, resolution 1.8Å" />	+
-	'''LEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH 2-PROPENYL-NEU5AC, AN INACTIVE SUBSTRATE ANALOGUE'''~~<br />~~	+	{{Structure
		+	\|PDB= 4sli \|SIZE=350\|CAPTION= <scene name='initialview01'>4sli</scene>, resolution 1.8Å
		+	\|SITE=
		+	\|LIGAND= <scene name='pdbligand=CNP:2-PROPENYL-N-ACETYL-NEURAMIC ACID'>CNP</scene>
		+	\|ACTIVITY= [http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18]
		+	\|GENE= T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6405 Macrobdella decora])
		+	}}
		+
		+	'''LEECH INTRAMOLECULAR TRANS-SIALIDASE COMPLEXED WITH 2-PROPENYL-NEU5AC, AN INACTIVE SUBSTRATE ANALOGUE'''
		+

	==Overview==		==Overview==
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	==About this Structure==		==About this Structure==
-	4SLI is a [~~http://en.wikipedia.org/wiki/Single_protein~~ Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Macrobdella_decora Macrobdella decora] ~~with <scene name='pdbligand=CNP:'>CNP</scene> as [http://en~~.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4SLI OCA].	+	4SLI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Macrobdella_decora Macrobdella decora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4SLI OCA].

	==Reference==		==Reference==
-	The 1.8 A structures of leech intramolecular trans-sialidase complexes: evidence of its enzymatic mechanism., Luo Y, Li SC, Li YT, Luo M, J Mol Biol. 1999 Jan 8;285(1):323-32. PMID:[http://~~ispc~~.~~weizmann~~.ac.~~il/~~/~~pmbin~~/~~getpm?pmid=~~9878409 9878409]	+	The 1.8 A structures of leech intramolecular trans-sialidase complexes: evidence of its enzymatic mechanism., Luo Y, Li SC, Li YT, Luo M, J Mol Biol. 1999 Jan 8;285(1):323-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9878409 9878409]
	[[Category: Exo-alpha-sialidase]]		[[Category: Exo-alpha-sialidase]]
	[[Category: Macrobdella decora]]		[[Category: Macrobdella decora]]
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	[[Category: intramolecular trans-sialidase]]		[[Category: intramolecular trans-sialidase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu ~~Feb 21~~ 19:14:24 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:10:57 2008''